The discovery of RNA-guided nucleases have enabled to leap forward in genome editing of cells and organisms. These nucleases can be delivered into cells as plasmid DNA, mRNA or ribonucleoprotein ...complexes (RNPs). The delivery in the form of RNP has some advantages because the target gene editing begins immediately without the process of intracellular synthesis of components and CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 (CRISPR associated protein 9) system assembly. This strategy makes it possible to directly control RNP concentration and to decrease the number of off-targets due to rapid degradation of the complex in the cell. However, the task to develop RNP delivery systems remains unsolved. This review is devoted to RNP delivery into cells and tissues using physical approaches and different carriers. Special attention is paid to novel approaches that improve the RNP delivery efficiency.
•The new carbacylamidophosphate was tested as ligand towards lanthanides.•The new CAPh based lanthanide complexes NEt4LnL4 were synthesized.•The CAPh and its europium complex crystal structures were ...solved.•Eu3+ and Tb3+ f-f luminescence with lifetime 2.10 and 2.38 ms was observed.•NO2-group of aromatic substitute of the CAPh leads to excitation energy dissipation.
O,O′-dimethyl-N-(3-nitrobenzoyl)amidophosphate (HL) was synthesized, structurally characterized, tested as ligand to obtain lanthanide complexes NEt4LnL4 (Ln = La3+, Ce3+, Pr3+, Nd3+, Sm3+, Eu3+, Gd3+, Tb3+, Dy3+, Er3+, Y3+) and as a sensitizer of Eu3+ and Tb3+ luminescence. It was shown that besides of typical for carbacylamidophosphates intermolecular contacts, the electrostatic interactions N∙∙∙O of the length 2.936 Å exist in the crystal of NEt4EuL4 and influence on both the crystal packing and the lanthanide polyhedron shape. In the structure of the complex anion, two pairs of symmetry-related ligands differ in configuration (syn- or anti-) of nitro groups with respect to the carbonyl groups. The obtained complexes were found to be thermally stable to near 200 °C. Luminescent studies have shown that excitation energy is dissipated, which prevents ligands from efficient luminescence sensitization of europium and terbium.
The preliminary results of measurement of the
e
+
e
−
→ π
+
π
−
π
0
cross section in the 1.05–2 GeV center-of-mass energy range with 30 pb
–1
of experimental data collected using the CMD-3 detector ...are presented. The parameters of resonant states ω(1420) and ω(1650) are obtained.
The process
e
+
e
−
→
K
+
K
−
π
0
has been studied in the center-of-mass energy range from 1.2 to 2 GeV using a 80.6 pb
−1
data sample collected with the CMD-3 detector at the electron-positron ...collider VEPP-2000. Preliminary results of the cross-section measurement are presented.
Recent results from CMD-3 Ignatov, F. V.; Akhmetshin, R. R.; Amirkhanov, A. N. ...
EPJ Web of Conferences,
2019, Volume:
212
Journal Article, Conference Proceeding
Peer reviewed
Open access
Regular data taking with the CMD-3 at the electron-positron collider VEPP-2000 is under way since 2010. The collected data sample corresponds to about 200 inverse picobarns of integrated luminosity ...per detector in the energy range from 0.32 up to 2 GeV, with a goal to collect about 1
fb
−1
during next five years. Some of the recent results from the CMD-3 detector are discussed.
New lanthanide dimethyl-N-benzoylamidophosphate (HL) based tetrakis-complexes NEt
LnL
(Ln
=La, Nd, Sm, Eu, Gd, Tb, Dy) are reported. The complexes are characterized by means of NMR, IR, absorption, ...and luminescent spectroscopy as well as by elemental, X-Ray, and thermal gravimetric analyses. The phenyl groups of the four ligands of the complex anion are directed towards one side, while the methoxy groups are directed in the opposite side, which makes the complexes under consideration structurally similar to calixarenes. The effect of changing the alkali metal counterion to the organic cation NEt
on the structure and properties of the tetrakis-complex LnL4
is analyzed. The complexes exhibit bright characteristic for respective lanthanides luminescence. Rather high intensity of the band of
D
→
F
transition, observed in the luminescence spectrum of NEt
EuL
, is discussed based on theoretical calculations.
We present current status of the SND and CMD-3 experiments at the VEPP-2000
e
+
e
−
collider and recent result of data analysis on the processes $e^+e^-\rightarrow\pi^+\pi^-,n\overline ...n,\pi^+\pi^-\eta,\pi^+\pi^-\pi^0\eta,K_SK_L\pi^0$, etc.
Binuclear rare earth complexes Ln
2
L
3
phen
2
(Ln
III
= Nd
III
, Sm
III
, Eu
III
, Tb
III
, Dy
III
, Yb
III
and Y
III
) with bis-CAPh type ligand - tetramethyl ...N,N′-(2,2,3,3,4,4-hexafluoro-1,5-dioxopentane-1,5-diyl)bis(phosphoramidate) (H
2
L) and 1,10-phenanthroline (phen) were synthesized and characterized by elemental analysis, IR, NMR, absorption and luminescence spectroscopy. Luminescence measurements were performed for all the complexes in solid state and for the Eu
III
, Tb
III
and Y
III
complexes - in solution in DMSO as well. The effective energy transfer from organic ligands to Ln
III
ions strongly sensitizes the Ln
III
ions emission and under excitation by UV light, the complexes exhibited bright characteristic emission of lanthanide metal centers. It was found that the energy level of the ligands lowest triplet state in the complexes matches better to resonance level of Eu
III
rather than Tb
III
ion. Depending on temperature the emission decay times of solid europium and terbium complexes were in the range of 1.5–2.0 ms. In solid state at room temperature the Eu
III
complex possess intense luminescence with very high intrinsic quantum yield 91% and decay time equal 1.88 ms.
We report preliminary results on the cross section of the process e
+
e
−
→ φ(1020)η measured at 30 center-of-mass energy points in the range from 1.59 up to 2.0 GeV. Data analysis is based on the ...integrated luminosity of 22 pb
−1
collected with the CMD-3 detector in 2011–2012. The obtained cross section agrees with the BaBar measurement and has better statistical accuracy.
The preliminary results of measurement of the e{sup +} e{sup −} → π{sup +}π{sup −}π{sup 0} cross section in the 1.05–2 GeV center-of-mass energy range with 30 pb{sup –1} of experimental data ...collected using the CMD-3 detector are presented. The parameters of resonant states ω(1420) and ω(1650) are obtained.
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