Mass spectrometry has enabled the study of cellular signaling on a systems-wide scale, through the quantification of post-translational modifications, such as protein phosphorylation. Here we ...describe EasyPhos, a scalable phosphoproteomics platform that now allows rapid quantification of hundreds of phosphoproteomes in diverse cells and tissues at a depth of >10,000 sites. We apply this technology to generate time-resolved maps of insulin signaling in the mouse liver. Our results reveal that insulin affects ~10% of the liver phosphoproteome and that many known functional phosphorylation sites, and an even larger number of unknown sites, are modified at very early time points (<15 s after insulin delivery). Our kinetic data suggest that the flow of signaling information from the cell surface to the nucleus can occur on very rapid timescales of less than 1 min in vivo. EasyPhos facilitates high-throughput phosphoproteomics studies, which should improve our understanding of dynamic cell signaling networks and how they are regulated and dysregulated in disease.
We calculate the yield and elliptic flow of midrapidity dileptons emitted from the quark-gluon plasma generated in Pb-Pb collisions at LHC. We use relativistic anisotropic hydrodynamics for the ...3+1-dimensional evolution of the quark-gluon plasma and convolve this with the momentum-anisotropic local rest frame production rate for dileptons. The effects of momentum anisotropy of the quark distribution functions, viscosity to entropy density ratio, centrality of the collisions, and initial momentum anisotropy on the results are investigated and discussed.
The emission of real photons from a momentum-anisotropic quark-gluon plasma (QGP) is affected by both the collective flow of the radiating medium and the modification of the local rest frame emission ...rate due to the anisotropic momentum distribution of partonic degrees of freedom. In this paper, we first calculate the photon production rate from an ellipsoidally momentum-anisotropic QGP including hard contributions from Compton scattering and quark pair annihilation and soft contribution calculated using the hard thermal loop approximation. We introduce a parametrization of the nonequilibrium rate in order to facilitate its further application in yield and flow calculations. We convolve the anisotropic photon rate with the space-time evolution of QGP provided by 3 + 1d anisotropic hydrodynamics to obtain the yield and the elliptic flow coefficient v2 of photons from QGP generated at Pb-Pb collisions at the LHC at 2.76 TeV and Au-Au collisions at the RHIC at 200 GeV. We investigate the effects of various parameters on the results. In particular, we analyze the sensitivity of the results to initial momentum anisotropy.
A
bstract
We extract the imaginary part of the heavy-quark potential using classical-statistical simulations of real-time Yang-Mills dynamics in classical thermal equilibrium. The
r
-dependence of ...the imaginary part of the potential is extracted by measuring the temporal decay of Wilson loops of spatial length
r
. We compare our results to continuum expressions obtained using hard thermal loop theory and to semi-analytic lattice perturbation theory calculations using the hard classical loop formalism. We find that, when plotted as a function of
m
D
r
, where
m
D
is the hard classical loop Debye mass, the imaginary part of the heavy-quark potential shows little sensitivity to the lattice spacing at small
m
D
r
≲ 1 and agrees well with the semi-analytic hard classical loop result. For large quark-antiquark separations, we quantify the magnitude of the non-perturbative long-range corrections to the imaginary part of the heavy-quark potential. We present our results for a wide range of temperatures, lattice spacings, and lattice volumes. This work sets the stage for extracting the imaginary part of the heavy-quark potential in an expanding non-equilibrium Yang Mills plasma.
This letter presents a fully-integrated 2-bit CMOS voltage-controlled oscillator (VCO) along with a 2-bit buffer, which delivers the highest RF output power among recently reported state-of-the-art ...to the best of the authors' knowledge. The proposed 2-bit VCO is designed and fabricated in a <inline-formula> <tex-math notation="LaTeX">0.18-\mu \text{m} </tex-math></inline-formula> CMOS process with a die size of 1.2 mm <inline-formula> <tex-math notation="LaTeX">\times </tex-math></inline-formula> 1.2 mm, which covers four frequency bands of interest. Fine-tuning at each of the four frequency bands is also obtained by utilizing two varactors. The proposed 2-bit VCO delivers a single-ended output power of 6.5/6.8/10.6/12.5 dBm at 0.930/1.157/1.436/1.917 GHz, respectively. This 2-bit VCO can cover frequency ranges of 930-1005, 1046-1157, 1248-1436, and 1540-1917 MHz, thus providing a total frequency bandwidth of 751 MHz. The proposed 2-bit VCO at each frequency band demonstrates −99, −93, −90, and −86 dBc/Hz for phase noise, and −150, −150, −160, and −168 dBc/Hz for figure-of-merit (FOM), respectively.
We introduce an efficient general method for calculating the self-energies, collective modes, and dispersion relations of quarks and gluons in a momentum-anisotropic high-temperature quark-gluon ...plasma. The method introduced is applicable to the most general classes of deformed anisotropic momentum distributions and the resulting self-energies are expressed in terms of a series of hypergeometric basis functions which are valid in the entire complex phase-velocity plane. Comparing to direct numerical integration of the self-energies, the proposed method is orders of magnitude faster and provides results with similar or better accuracy. To extend previous studies and demonstrate the application of the proposed method, we present numerical results for the parton self-energies and dispersion relations of partonic collective excitations for the case of an ellipsoidal momentum-space anisotropy. Finally, we also present, for the first time, the gluon unstable mode growth rate for the case of an ellipsoidal momentum-space anisotropy.
In this work, we provide a unified and comparative description of the most prominent phase field based two-phase flow models and present our numerical results of the application of Galerkin-based ...Isogeometric Analysis (IGA) to incompressible Navier–Stokes–Cahn–Hilliard (NSCH) equations in velocity–pressure–phase field-chemical potential formulation. For the approximation of the velocity and pressure fields, LBB compatible non-uniform rational B-spline spaces are used which can be regarded as smooth generalizations of Taylor–Hood pairs of finite element spaces. The one-step θ-scheme is used for the discretization in time. The static and rising bubble, in addition to the nonlinear Rayleigh–Taylor instability flow problems, are considered in two dimensions as model problems in order to investigate the numerical properties of the scheme.
We present the results of the search for an isotropic stochastic gravitational wave background (GWB) at nanohertz frequencies using the second data release of the European Pulsar Timing Array (EPTA) ...for 25 millisecond pulsars and a combination with the first data release of the Indian Pulsar Timing Array (InPTA). A robust GWB detection is conditioned upon resolving the Hellings-Downs angular pattern in the pairwise cross-correlation of the pulsar timing residuals. Additionally, the GWB is expected to yield the same (common) spectrum of temporal correlations across pulsars, which is used as a null hypothesis in the GWB search. Such a common-spectrum process has already been observed in pulsar timing data. We analysed (i) the full 24.7-year EPTA data set, (ii) its 10.3-year subset based on modern observing systems, (iii) the combination of the full data set with the first data release of the InPTA for ten commonly timed millisecond pulsars, and (iv) the combination of the 10.3-year subset with the InPTA data. These combinations allowed us to probe the contributions of instrumental noise and interstellar propagation effects. With the full data set, we find marginal evidence for a GWB, with a Bayes factor of four and a false alarm probability of 4%. With the 10.3-year subset, we report evidence for a GWB, with a Bayes factor of 60 and a false alarm probability of about 0.1% (≳3
σ
significance). The addition of the InPTA data yields results that are broadly consistent with the EPTA-only data sets, with the benefit of better noise modelling. Analyses were performed with different data processing pipelines to test the consistency of the results from independent software packages. The latest EPTA data from new generation observing systems show non-negligible evidence for the GWB. At the same time, the inferred spectrum is rather uncertain and in mild tension with the common signal measured in the full data set. However, if the spectral index is fixed at 13/3, the two data sets give a similar amplitude of (2.5 ± 0.7) × 10
−15
at a reference frequency of 1 yr
−1
. Further investigation of these issues is required for reliable astrophysical interpretations of this signal. By continuing our detection efforts as part of the International Pulsar Timing Array (IPTA), we expect to be able to improve the measurement of spatial correlations and better characterise this signal in the coming years.
OBJECT Patients undergoing craniotomy are at risk for developing venous thromboembolism (VTE). The safety of anticoagulation in these patients is not clear. The authors sought to identify risk ...factors predictive of VTE in patients undergoing craniotomy. METHODS The authors reviewed a national surgical quality database, the American College of Surgeons National Surgical Quality Improvement Program. Craniotomy patients were identified by current procedural terminology code. Clinical factors were analyzed to identify associations with VTE. RESULTS Four thousand eight hundred forty-four adult patients who underwent craniotomy were identified. The rate of VTE in the cohort was 3.5%, including pulmonary embolism in 1.4% and deep venous thrombosis in 2.6%. A number of factors were found to be statistically significant in multivariate binary logistic regression analysis, including craniotomy for tumor, transfer from acute care hospital, age ≥ 60 years, dependent functional status, tumor involving the CNS, sepsis, emergency surgery, surgery time ≥ 4 hours, postoperative urinary tract infection, postoperative pneumonia, on ventilator ≥ 48 hours postoperatively, and return to the operating room. Patients were assigned a score based on how many of these factors they had (minimum score 0, maximum score 12). Increasing score was predictive of increased VTE incidence, as well as risk of mortality, and time from surgery to discharge. CONCLUSIONS Patients undergoing craniotomy are at low risk of developing VTE, but this risk is increased by preoperative medical comorbidities and postoperative complications. The presence of more of these clinical factors is associated with progressively increased VTE risk; patients possessing a VTE Risk Score of ≥ 5 had a greater than 20-fold increased risk of VTE compared with patients with a VTE score of 0.
What Do You Mean, ‘Tipping Point’? van Nes, Egbert H.; Arani, Babak M.S.; Staal, Arie ...
Trends in ecology & evolution (Amsterdam),
12/2016, Volume:
31, Issue:
12
Journal Article
Peer reviewed
Over the past 10 years the use of the term ‘tipping point’ in the scientific literature has exploded. It was originally used loosely as a metaphor for the phenomenon that, beyond a certain threshold, ...runaway change propels a system to a new state. Although several specific mathematical definitions have since been proposed, we argue that these are too narrow and that it is better to retain the original definition.
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