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•A fast and simple synthesis of Carbon Dots from lemon bagasse (CD-L)•Chemical, morphological and optical properties for CD-L was investigated.•CD-L revealed significant anti-oxidant ...potential.•A methodology for obtaining carbon dots film (Film-L) was developed.•Film-L has been evaluated as an efficient luminescent thermometer.
The design of luminescent nanomaterials for the development of nanothermometers with high sensitivity and free of potentially toxic metals has developed in several fields, such as optoelectronics, sensors, and bioimaging. In addition, luminescent nanothermometers have advantages related to non-invasive measurement, with their wide detection range and high spatial resolution at the nano/microscale. Our study is the first, to our knowledge, to demonstrate a detailed study of a fluorescent film (Film-L) thermal sensor based on carbon dots derived from lemon bagasse extract (CD-L). The CD-L properties were explored as an antioxidant agent; their cytotoxicity was evaluated by using a human non-tumoral skin fibroblast (HFF-1) cell line from an MTT assay. The CD-L were characterized by HRTEM, DLS, FTIR, UV–VIS, and fluorescence spectroscopy. These confirmed their particle size distribution below 10 nm, graphitic structure in the core and surface organic groups, and strong blue emission. The CD-L showed cytocompatibility behavior and scavenging potential reactive species of biological importance: O2•− and HOCl, with IC50 of 276.8 ± 4.0 and 21.6 ± 0.7, respectively. The Film-L emission intensities (I425 nm) are temperature-dependent in the 298 to 333 K range. The Film-L luminescent thermometer shows a maximum relative thermal sensitivity of 2.69 % K−1 at 333 K.
Deforestation threatens the integrity of the Amazon biome and the ecosystem services it provides, including greenhouse gas mitigation. Forest‐to‐pasture conversion has been shown to alter the flux of ...methane gas (CH4) in Amazonian soils, driving a switch from acting as a sink to a source of atmospheric CH4. This study aimed to better understand this phenomenon by investigating soil microbial metagenomes, focusing on the taxonomic and functional structure of methane‐cycling communities. Metagenomic data from forest and pasture soils were combined with measurements of in situ CH4 fluxes and soil edaphic factors and analysed using multivariate statistical approaches. We found a significantly higher abundance and diversity of methanogens in pasture soils. As inferred by co‐occurrence networks, these microorganisms seem to be less interconnected within the soil microbiota in pasture soils. Metabolic traits were also different between land uses, with increased hydrogenotrophic and methylotrophic pathways of methanogenesis in pasture soils. Land‐use change also induced shifts in taxonomic and functional traits of methanotrophs, with bacteria harbouring genes encoding the soluble form of methane monooxygenase enzyme (sMMO) depleted in pasture soils. Redundancy analysis and multimodel inference revealed that the shift in methane‐cycling communities was associated with high pH, organic matter, soil porosity and micronutrients in pasture soils. These results comprehensively characterize the effect of forest‐to‐pasture conversion on the microbial communities driving the methane‐cycling microorganisms in the Amazon rainforest, which will contribute to the efforts to preserve this important biome.
Ethics in care during the dying process: case report de Souza, Leilane Barbosa; de Souza, Luíza Eridan Elmiro Martins; Alves e Souza, Angela Maria
Revista brasileira de enfermagem,
2005 Nov-Dec, Volume:
58, Issue:
6
Journal Article
Peer reviewed
Open access
This study considers ethical issues related to the terminally ill. Its aims are to report the experience of home care and to promote a reflection about the ethical dilemma of caring the terminally ...ill. We concluded that the bioethical acting in this kind of situation involves a kind of conscience that can only be developed when the patient's reality is understood. We also realized the relevance of an interdisciplinary approach as a strategy for the holistic care to the terminally ill and the need to give attention to care when the cure is not possible, but the quality of life in the last moments of life.
This paper deals with the problem of admissibility analysis (i.e. regularity, causality and exponential stability) of discrete-time linear descriptor systems with uncertain time-varying parameters. ...The parameters enter affinely into the state matrix of the system state-space model, and their admissible values and variations are assumed to belong to given intervals. First, necessary and sufficient admissibility conditions for uncertainty-free discrete linear time-varying descriptor systems are presented. Next, strict LMI conditions based on parameter-dependent Lyapunov functions are proposed to ensure robust admissibility of uncertain descriptor systems. Both the cases of Lyapunov functions with affine and quadratic dependence on the system uncertain parameters are considered. The robust admissibility analysis methods incorporate information on available bounds on both the admissible values and variation of the uncertain parameters. Numerical examples are presented to demonstrate the potentials of the proposed methods.
A automedicação na gestação representa um problema para a saúde pública global, visto que grande parte dos fármacos são capazes de transpor a barreira placentária e a maioria não foi testada ...clinicamente em gestantes. A exposição às drogas, seja de origem sintética ou natural, durante a gravidez, envolve riscos à mãe e ao feto, o que torna necessário a realização de estudos sobre a temática. O objetivo deste estudo é analisar na literatura científica se a automedicação é praticada por mulheres no período gestacional e quais fatores estão relacionados a esse fenômeno. Este estudo é uma revisão integrativa onde foi realizada busca nas bases de dados científicas Scientific Electronic Library Online (SCIELO) e na coleção da Biblioteca Virtual da Saúde (BVS) sob a ótica da estratégia PICO. Após aplicação dos critérios de inclusão e exclusão durante a identificação, seleção, elegibilidade e inclusão das pesquisas, compôs a amostra da presente revisão 12 estudos. A presença de automedicação entre gestante foi identificada em todos os estudos avaliados. Pouco conhecimento sobre riscos, vulnerabilidade econômica, poucas consultas de pré-natal, crenças e fácil acesso às medicações sintéticas ou plantas medicinais, foram alguns dos fatores relacionados à automedicação entre este público. A educação em saúde durante o acompanhamento de pré-natal possui poder de impacto positivo na não automedicação. Por tanto, o pré-natal é um fator de proteção da não automedicação entre as gestantes, sendo a enfermagem uma profissão essencial para a prevenção deste comportamento em vista da sua participação ativa neste processo.
O Brasil classifica o aborto como crime conforme o Código Penal de 1940, entretanto, essa legislação não reflete as mudanças sociais contemporâneas e pode prejudicar a autonomia das mulheres. Sob tal ...ótica, este artigo tem por objetivo compreender como o estudo da Criminologia Feminista Marxista pode contribuir para o debate sobre a descriminalização do aborto. O presente estudo consistiu em análise bibliográfica de livros, artigos e revistas literárias, utilizando-se do método dedutivo para avaliar a situação geral do tema em questão. O estudo consistiu em uma pesquisa qualitativa. A análise se estende à discussão entre o Código Penal e os Direitos Humanos, examinando as implicações da criminalização do aborto à luz desses direitos. Posteriormente, são problematizados os conceitos formulados pela vertente feminista, especialmente no que diz respeito ao estudo de gênero e ao aborto, enfatizando a importância da autodeterminação da mulher. Os conceitos e teorias fundamentais da criminologia feminista marxista ressalta a análise crítica das desigualdades de gênero e poder. Por fim, o artigo relaciona a discussão sobre a descriminalização do aborto à perspectiva criminológica marxista, sugerindo uma abordagem que considera as estruturas sociais e econômicas subjacentes. Após a análise tornou-se evidente que a legislação vigente não apenas se revela ineficaz na diminuição do número de abortos, mas também restringe a autonomia das mulheres para exercerem seus direitos sexuais e reprodutivos, conforme estipulado pela lei. Acredita-se que esta pesquisa pode propiciar reflexão acerca da descriminalização do aborto no Brasil em comparação com a legislação. Complementa-se, que o presente estudo possa servir de inspiração para estudos mais avançados sobre o tema.
Brazil classifies abortion as a crime under the 1940 Penal Code. However, this legislation does not reflect contemporary social changes and can harm women's autonomy. From this perspective, this article aims to understand how the study of Marxist Feminist Criminology can contribute to the debate on the decriminalization of abortion. The analysis extends to the discussion between the Penal Code and Human Rights, examining the implications of the criminalization of abortion in the light of these rights. Subsequently, the concepts formulated by the feminist strand are problematized, especially with regard to the study of gender and abortion, emphasizing the importance of women's self-determination. The fundamental concepts and theories of Marxist feminist criminology highlight the critical analysis of gender and power inequalities. Finally, the article relates the discussion on the decriminalization of abortion to the Marxist criminological perspective, suggesting an approach that considers the underlying social and economic structures. This study consisted of a bibliographical analysis of books, articles and literary magazines, using the deductive method to assess the general situation of the issue in question. The study consisted of qualitative research. After analysis, it became clear that current legislation is not only ineffective in reducing the number of abortions, but also restricts women's autonomy to exercise their sexual and reproductive rights, as stipulated by law. It is believed that this research can encourage reflection on the decriminalization of abortion in Brazil in comparison with the legislation. In addition, this study may serve as inspiration for more advanced studies on the subject.
Brasil tipifica el aborto como delito en el Código Penal de 1940. Sin embargo, esta legislación no refleja los cambios sociales contemporáneos y puede perjudicar la autonomía de las mujeres. Desde esta perspectiva, este artículo pretende entender cómo el estudio de la Criminología Feminista Marxista puede contribuir al debate sobre la despenalización del aborto. El análisis se extiende a la discusión entre el Código Penal y los Derechos Humanos, examinando las implicaciones de la criminalización del aborto a la luz de estos derechos. Posteriormente, se problematizan los conceptos formulados por la vertiente feminista, especialmente en lo que se refiere al estudio de género y aborto, enfatizando la importancia de la autodeterminación de las mujeres. Los conceptos y teorías fundamentales de la criminología feminista marxista destacan el análisis crítico de las desigualdades de género y de poder. Finalmente, el artículo relaciona la discusión sobre la despenalización del aborto con la perspectiva criminológica marxista, sugiriendo un abordaje que considere las estructuras sociales y económicas subyacentes. El estudio consistió en un análisis bibliográfico de libros, artículos y revistas literarias, utilizando el método deductivo para evaluar la situación general del tema en cuestión. El estudio consistió en una investigación cualitativa. Tras el análisis, quedó claro que la legislación actual no sólo es ineficaz para reducir el número de abortos, sino que también restringe la autonomía de las mujeres para ejercer sus derechos sexuales y reproductivos, tal y como estipula la ley. Se cree que esta investigación puede fomentar la reflexión sobre la despenalización del aborto en Brasil en comparación con la legislación. Además, este estudio puede servir de inspiración para estudios más avanzados sobre el tema.
Human T-lymphotropic virus 1 (HTLV-1) is associated with the development of several pathologies and chronic infection in humans. The inefficiency of the available treatments and the challenge in ...developing a protective vaccine highlight the need to produce effective immunotherapeutic tools. The HTLV-1 basic leucine zipper (bZIP) factor (HBZ) plays an important role in the HTLV-1 persistence, conferring a survival advantage to infected cells by reducing the HTLV-1 proteins expression, allowing infected cells to evade immune surveillance, and enhancing cell proliferation leading to increased proviral load.
We have generated a recombinant Modified Virus Vaccinia Ankara (MVA-HBZ) and a plasmid DNA (pcDNA3.1(+)-HBZ) expressing a multiepitope protein based on peptides of HBZ to study the immunogenic potential of this viral-derived protein in BALB/c mice model. Mice were immunized in a prime-boost heterologous protocol and their splenocytes (T CD4
and T CD8
) were immunophenotyped by flow cytometry and the humoral response was evaluated by ELISA using HBZ protein produced in prokaryotic vector as antigen.
T CD4
and T CD8
lymphocytes cells stimulated by HBZ-peptides (HBZ
and HBZ
) showed polyfunctional double positive responses for TNF-α/IFN-γ, and TNF-α/IL-2. Moreover, T CD8
cells presented a tendency in the activation of effector memory cells producing granzyme B (CD44
/CD62L
), and the activation of Cytotoxic T Lymphocytes (CTLs) and cytotoxic responses in immunized mice were inferred through the production of granzyme B by effector memory T cells and the expression of CD107a by CD8
T cells. The overall data is consistent with a directive and effector recall response, which may be able to operate actively in the elimination of HTLV-1-infected cells and, consequently, in the reduction of the proviral load. Sera from immunized mice, differently from those of control animals, showed IgG-anti-HBZ production by ELISA.
Our results highlight the potential of the HBZ multiepitope protein expressed from plasmid DNA and a poxviral vector as candidates for therapeutic vaccine.
Uniplant, a single Silastic implant containing nomegestrol acetate, provides contraceptive efficacy for one year. Uniplant use for one year was studied in 1,803 healthy women of reproductive age, ...enrolled from 10 centers in 9 countries, after informed consent. Implants were placed subdermally either in the upper arm or in the gluteal region. Two-hundred-seventy-six subjects discontinued prior to completing one year of study. Cumulative discontinuation rate at 12 months was 15.72%. Medical reasons for discontinuation were principally menstrual-related. Fifteen pregnancies occurred during the one year study period, resulting in a 12-month net cumulative pregnancy rate of 0.94%. Approximately 56% of subjects using Uniplant had bleeding patterns similar to normal menstruation. Results from this study confirm that Uniplant is an efficient, well tolerated, 12-month contraceptive implant, with the advantage of easier insertion and removal of the single implant compared to other multiple implant methods.
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