The Radiation Belt Storm Probes (RBSP) Education and Public Outreach (E/PO) program serves as a pipeline of activities to inspire and educate a broad audience about Heliophysics and the Sun-Earth ...system, specifically the Van Allen Radiation Belts. The program is comprised of a variety of formal, informal and public outreach activities that all align with the NASA Education Portfolio Strategic Framework outcomes. These include lesson plans and curriculum for use in the classroom, teacher workshops, internship opportunities, activities that target underserved populations, collaboration with science centers and NASA visitors’ centers and partnerships with experts in the Heliophysics and education disciplines. This paper will detail the activities that make up the RBSP E/PO program, their intended audiences, and an explanation as to how they align with the NASA education outcomes. Additionally, discussions on why these activities are necessary as part of a NASA mission are included. Finally, examples of how the RBSP E/PO team has carried out some of these activities will be discussed throughout.
The Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) is a hyperspectral imager on the Mars Reconnaissance Orbiter (MRO) spacecraft. CRISM consists of three subassemblies, a gimbaled ...Optical Sensor Unit (OSU), a Data Processing Unit (DPU), and the Gimbal Motor Electronics (GME). CRISM's objectives are (1) to map the entire surface using a subset of bands to characterize crustal mineralogy, (2) to map the mineralogy of key areas at high spectral and spatial resolution, and (3) to measure spatial and seasonal variations in the atmosphere. These objectives are addressed using three major types of observations. In multispectral mapping mode, with the OSU pointed at planet nadir, data are collected at a subset of 72 wavelengths covering key mineralogic absorptions and binned to pixel footprints of 100 or 200 m/pixel. Nearly the entire planet can be mapped in this fashion. In targeted mode the OSU is scanned to remove most along‐track motion, and a region of interest is mapped at full spatial and spectral resolution (15–19 m/pixel, 362–3920 nm at 6.55 nm/channel). Ten additional abbreviated, spatially binned images are taken before and after the main image, providing an emission phase function (EPF) of the site for atmospheric study and correction of surface spectra for atmospheric effects. In atmospheric mode, only the EPF is acquired. Global grids of the resulting lower data volume observations are taken repeatedly throughout the Martian year to measure seasonal variations in atmospheric properties. Raw, calibrated, and map‐projected data are delivered to the community with a spectral library to aid in interpretation.
The STEREO mission’s Education and Outreach (E/PO) program began early enough its team benefited from many lessons learned as NASA’s E/PO profession matured. Originally made up of discrete programs, ...by launch the STEREO E/PO program had developed into a quality suite containing all the program elements now considered standard: education workshops, teacher/student guides, national and international collaboration, etc. The benefit of bringing so many unique programs together is the resulting diverse portfolio, with scientists, E/PO professionals, and their education partners all of whom can focus on excellent smaller programs. The drawback is a less cohesive program nearly impossible to evaluate in its entirety with the given funding. When individual components were evaluated, we found our programs mostly made positive impact. In this paper, we elaborate on the programs, hoping that others will effectively use or improve upon them. When possible, we indicate the programs’ effects on their target audiences.
Water-soluble poly(oligo(ethylene glycol) methacrylate) (poly(OEGMA)) with various main chain and side chain lengths were grafted to polyurethane (PU) surface by surface-initiated atom transfer ...radical graft polymerization (s-ATRgP). The polymer main chain length was varied by varying the molar ratio of monomer to free initiator in solution (typically 5:1, 50:1, 100:1). Three different side chain lengths were obtained using different OEGMA monomers (MW 300, 475, 1100
g/mol). Water contact angle and X-ray photoelectron spectroscopy (XPS) were used to characterize the modified PU surfaces. The respective effects of poly(OEGMA) main chain and side chain lengths on fibrinogen (Fg) and lysozyme (Lys) adsorption were investigated in single protein systems at room temperature in TBS, pH 7.4. The poly(OEGMA)-grafted PU surfaces were found to be highly protein-resistant, with reductions of Fg and Lys adsorption in the range of 84–98% and 67–91%, respectively, compared to the unmodified PU surface. The adsorption of both proteins decreased with increasing poly(OEGMA) main chain length for a given side chain length (number of EO units). For a given main chain length, the Fg adsorption level did not change significantly with increasing side chain length. However, Lys adsorption increased with increasing side chain length, possibly due to decreasing graft density as monomer size and footprint on the surface increase. Adsorption resistance was generally greater for the bigger protein.
Canine hematopoietic progenitor cells were characterized by separation with monoclonal antibodies. Depleted and enriched fractions were studied for growth of CFU-GM in semisolid agar and for ...repopulating capacity of lethally irradiated dogs. CFU growth was not reduced by depletion of marrow using monoclonal antibodies F 3-20-7 (anti-dog Thy-1), MT606 (anti-human CD 6), and IOT2a (anti-human DR). CFU growth was variable following treatment with the anti-canine T-cell antibody MdT-P 1 and immunomagnetic bead separation. It was regularly enriched when MdT-P 1 treatment was followed by immunorosetting with staphylococcal protein A-loaded sheep red blood cells and density gradient separation. Lethally irradiated dogs were reconstituted by autologous marrow depleted of MdT-P 1-positive cells using immunorosetting and density gradient centrifugation, whereas immunomagnetic bead-depleted marrow was ineffective. Fluorescence-activated cell sorting showed enrichment of hematopoietic progenitor cells in the weakly MdT-P 1-positive fraction.
Functional characterization of subsets of T lymphocytes is essential for transplantation studies in dogs, as it is in other species. We studied the function of T cells separated by two mouse ...monoclonal antibodies recognizing complementary subsets--an antibody directed to canine T cells (MdT-P1) with an up-regulating function, and an antibody directed to human CD 8 (MT811) that cross-reacts with down-regulating canine T cells. Immunorosetting with sheep red blood cells and Percoll gradient allowed us to study depleted and enriched fractions. Their function was tested in mixed lymphocyte culture (MLC), cell-mediated cytotoxicity (CML), and coculture with B cells in a hemolytic plaque assay (PFC). In MLC, MdT-P1-positive cells showed a high proliferative response, and MT811-positive cells responded poorly to allogeneic cells. Vice versa, MT811- negative cells responded strongly, and MdT-P1-negative cells were poor responders but strong stimulators. Effector cells of CML were separated following 8 days of culture and prior to mixing with target cells. Enriched and depleted fractions with either antibody showed low cytotoxic activity as compared with unseparated cells. When added to unseparated effector cells MT 811-positive cells suppressed cytotoxicity. B cells were obtained by rosetting with staphylococcal protein A (SPA). Their immunoglobulin production was studied following 6 days of culture stimulated by pokeweed mitogen in a reverse hemolytic plaque assay. Again, MT 811-positive cells added to the culture suppressed, and MT 811-negative cells enhanced immunoglobulin production. In conclusion, immunorosetting with two monoclonal antibodies allowed us to distinguish subpopulations of canine T cells with up-regulating (helper/inducer) from those with down-regulating (suppressor) activity.
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