To understand how the brain processes sensory information to guide behavior, we must know how stimulus representations are transformed throughout the visual cortex. Here we report an open, ...large-scale physiological survey of activity in the awake mouse visual cortex: the Allen Brain Observatory Visual Coding dataset. This publicly available dataset includes the cortical activity of nearly 60,000 neurons from six visual areas, four layers, and 12 transgenic mouse lines in a total of 243 adult mice, in response to a systematic set of visual stimuli. We classify neurons on the basis of joint reliabilities to multiple stimuli and validate this functional classification with models of visual responses. While most classes are characterized by responses to specific subsets of the stimuli, the largest class is not reliably responsive to any of the stimuli and becomes progressively larger in higher visual areas. These classes reveal a functional organization wherein putative dorsal areas show specialization for visual motion signals.
The anatomy of the mammalian visual system, from the retina to the neocortex, is organized hierarchically
. However, direct observation of cellular-level functional interactions across this hierarchy ...is lacking due to the challenge of simultaneously recording activity across numerous regions. Here we describe a large, open dataset-part of the Allen Brain Observatory
-that surveys spiking from tens of thousands of units in six cortical and two thalamic regions in the brains of mice responding to a battery of visual stimuli. Using cross-correlation analysis, we reveal that the organization of inter-area functional connectivity during visual stimulation mirrors the anatomical hierarchy from the Allen Mouse Brain Connectivity Atlas
. We find that four classical hierarchical measures-response latency, receptive-field size, phase-locking to drifting gratings and response decay timescale-are all correlated with the hierarchy. Moreover, recordings obtained during a visual task reveal that the correlation between neural activity and behavioural choice also increases along the hierarchy. Our study provides a foundation for understanding coding and signal propagation across hierarchically organized cortical and thalamic visual areas.
Peatlands contain approximately one third of all soil organic carbon (SOC). Warming can alter above‐ and belowground linkages that regulate soil organic carbon dynamics and C‐balance in peatlands. ...Here we examine the multiyear impact of in situ experimental warming on the microbial food web, vegetation, and their feedbacks with soil chemistry. We provide evidence of both positive and negative impacts of warming on specific microbial functional groups, leading to destabilization of the microbial food web. We observed a strong reduction (70%) in the biomass of top‐predators (testate amoebae) in warmed plots. Such a loss caused a shortening of microbial food chains, which in turn stimulated microbial activity, leading to slight increases in levels of nutrients and labile C in water. We further show that warming altered the regulatory role of Sphagnum‐polyphenols on microbial community structure with a potential inhibition of top predators. In addition, warming caused a decrease in Sphagnum cover and an increase in vascular plant cover. Using structural equation modelling, we show that changes in the microbial food web affected the relationships between plants, soil water chemistry, and microbial communities. These results suggest that warming will destabilize C and nutrient recycling of peatlands via changes in above‐ and belowground linkages, and therefore, the microbial food web associated with mosses will feedback positively to global warming by destabilizing the carbon cycle. This study confirms that microbial food webs thus constitute a key element in the functioning of peatland ecosystems. Their study can help understand how mosses, as ecosystem engineers, tightly regulate biogeochemical cycling and climate feedback in peatlands
The visual world projects a complex and rapidly changing image onto the retina of many animal species. This presents computational challenges for those animals reliant on visual processing to provide ...an accurate representation of the world. One such challenge is parsing a visual scene for the most salient targets, such as the selection of prey amid a swarm. The ability to selectively prioritize processing of some stimuli over others is known as 'selective attention'. We recently identified a dragonfly visual neuron called 'Centrifugal Small Target Motion Detector 1' (CSTMD1) that exhibits selective attention when presented with multiple, equally salient targets. Here we conducted
, electrophysiological recordings from CSTMD1 in wild-caught male dragonflies (
), while presenting visual stimuli on an LCD monitor. To identify the target selected in any given trial, we uniquely modulated the intensity of the moving targets (frequency tagging). We found that the frequency information of the selected target is preserved in the neuronal response, while the distracter is completely ignored. We also show that the competitive system that underlies selection in this neuron can be biased by the presentation of a preceding target on the same trajectory, even when it is of lower contrast than an abrupt, novel distracter. With this improved method for identifying and biasing target selection in CSTMD1, the dragonfly provides an ideal animal model system to probe the neuronal mechanisms underlying selective attention.
We present the first application of frequency tagging to intracellular neuronal recordings, demonstrating that the frequency component of a stimulus is encoded in the spiking response of an
neuron. Using this technique as an identifier, we demonstrate that CSTMD1 'locks on' to a selected target and encodes the absolute strength of this target, even in the presence of abruptly appearing, high-contrast distracters. The underlying mechanism also permits the selection mechanism to switch between targets mid-trial, even among equivalent targets. Together, these results demonstrate greater complexity in this selective attention system than would be expected in a winner-takes-all network. These results are in contrast to typical findings in the primate and avian brain, but display intriguing resemblance to observations in human psychophysics.
We present the first survey of resolved stellar populations in the remote outer halo of our nearest giant elliptical (gE), Centaurus A (D = 3.8 Mpc). Using the VIsible Multi Object Spectrograph ...(VIMOS)/Very Large Telescope (VLT) optical camera, we obtained deep photometry for four fields along the major and minor axes at projected elliptical radii of ∼30-85 kpc (corresponding to ∼5-14R
eff). We use resolved star counts to map the spatial and colour distribution of red giant branch (RGB) stars down to ∼2 mag below the RGB tip. We detect an extended halo out to the furthermost elliptical radius probed (∼85 kpc or ∼14R
eff), demonstrating the vast extent of this system. We detect a localized substructure in these parts, visible in both (old) RGB and (intermediate-age) luminous asymptotic giant branch stars, and there is some evidence that the outer halo becomes more elliptical and has a shallower surface brightness profile. We derive photometric metallicity distribution functions for halo RGB stars and find relatively high median metallicity values (〈Fe/H〉med ∼ −0.9 to −1.0 dex) that change very little with radius over the extent of our survey. Radial metallicity gradients are measured to be −0.002-0.004 dex kpc−1, and the fraction of metal-poor stars (defined as Fe/H < −1.0) is 40-50 per cent at all radii. We discuss these findings in the context of galaxy formation models for the buildup of gE haloes.
The effect of age on the anatomy and function of the human colon is incompletely understood. The prevalence of disorders in adults such as constipation increase with age but it is unclear if this is ...due to confounding factors or age‐related structural defects. The aim of this study was to determine number and subtypes of enteric neurons and neuronal volumes in the human colon of different ages. Normal colon (descending and sigmoid) from 16 patients (nine male) was studied; ages 33–99. Antibodies to HuC/D, choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), and protein gene product 9.5 were used. Effect of age was determined by testing for linear trends using regression analysis. In the myenteric plexus, number of Hu‐positive neurons declined with age (slope = −1.3 neurons/mm/10 years, P = 0.03). The number of ChAT‐positive neurons also declined with age (slope = −1.1 neurons/mm/10 years of age, P = 0.02). The number of nNOS‐positive neurons did not decline with age. As a result, the ratio of nNOS to Hu increased (slope = 0.03 per 10 years of age, P = 0.01). In the submucosal plexus, the number of neurons did not decline with age (slope = −0.3 neurons/mm/10 years, P = 0.09). Volume of nerve fibres in the circular muscle and volume of neuronal structures in the myenteric plexus did not change with age. In conclusion, the number of neurons in the human colon declines with age with sparing of nNOS‐positive neurons. This change was not accompanied by changes in total volume of neuronal structures suggesting compensatory changes in the remaining neurons.
Visual cues provide an important means for aerial creatures to ascertain their self-motion through the environment. In many insects, including flies, moths, and bees, wide-field motion-sensitive ...neurons in the third optic ganglion are thought to underlie such motion encoding; however, these neurons can only respond robustly over limited speed ranges. The task is more complicated for some species of dragonflies that switch between extended periods of hovering flight and fast-moving pursuit of prey and conspecifics, requiring motion detection over a broad range of velocities. Since little is known about motion processing in these insects, we performed intracellular recordings from hawking, emerald dragonflies (
) and identified a diverse group of motion-sensitive neurons that we named lobula tangential cells (LTCs). Following prolonged visual stimulation with drifting gratings, we observed significant differences in both temporal and spatial tuning of LTCs. Cluster analysis of these changes confirmed several groups of LTCs with distinctive spatiotemporal tuning. These differences were associated with variation in velocity tuning in response to translated, natural scenes. LTCs with differences in velocity tuning ranges and optima may underlie how a broad range of motion velocities are encoded. In the hawking dragonfly, changes in LTC tuning over time are therefore likely to support their extensive range of behaviors, from hovering to fast-speed pursuits.
Understanding how animals navigate the world is an inherently difficult and interesting problem. Insects are useful models for understanding neuronal mechanisms underlying these activities, with neurons that encode wide-field motion previously identified in insects, such as flies, hawkmoths, and butterflies. Like some Dipteran flies, dragonflies exhibit complex aerobatic behaviors, such as hovering, patrolling, and aerial combat. However, dragonflies lack halteres that support such diverse behavior in flies. To understand how dragonflies might address this problem using only visual cues, we recorded from their wide-field motion-sensitive neurons. We found these differ strongly in the ways they respond to sustained motion, allowing them collectively to encode the very broad range of velocities experienced during diverse behavior.
ABSTRACT
We use the SMASH survey to obtain unprecedented deep photometry reaching down to the oldest main-sequence turn-offs in the colour–magnitude diagrams (CMDs) of the Small Magellanic Cloud ...(SMC) and quantitatively derive its star formation history (SFH) using CMD fitting techniques. We identify five distinctive peaks of star formation in the last 3.5 Gyr, at ∼3, ∼2, ∼1.1, ∼0.45 Gyr ago, and one presently. We compare these to the SFH of the Large Magellanic Cloud (LMC), finding unequivocal synchronicity, with both galaxies displaying similar periods of enhanced star formation over the past ∼3.5 Gyr. The parallelism between their SFHs indicates that tidal interactions between the MCs have recurrently played an important role in their evolution for at least the last ∼3.5 Gyr, tidally truncating the SMC and shaping the LMC’s spiral arm. We show, for the first time, an SMC–LMC correlated SFH at recent times in which enhancements of star formation are localized in the northern spiral arm of the LMC, and globally across the SMC. These novel findings should be used to constrain not only the orbital history of the MCs but also how star formation should be treated in simulations.
In hemodialysis patients extracellular fluid overload is a predictor of all-cause and cardiovascular mortality, and a relation with inflammation has been reported in previous studies. The magnitude ...and nature of this interaction and the effects of moderate fluid overload and extracellular fluid depletion on survival are still unclear. We present the results of an international cohort study in 8883 hemodialysis patients from the European MONDO initiative database where, during a three-month baseline period, fluid status was assessed using bioimpedance and inflammation by C-reactive protein. All-cause mortality was recorded during 12 months of follow up. In a second analysis a three-month baseline period was added to the first baseline period, and changes in fluid and inflammation status were related to all-cause mortality during six-month follow up. Both pre-dialysis estimated fluid overload and fluid depletion were associated with an increased mortality, already apparent at moderate levels of estimated pre-dialysis fluid overload (1.1-2.5L); hazard ratio 1.64 (95% confidence interval 1.35-1.98). In contrast, post-dialysis estimated fluid depletion was associated with a survival benefit (0.74 0.62-0.90). The concurrent presence of fluid overload and inflammation was associated with the highest risk of death. Thus, while pre-dialysis fluid overload was associated with inflammation, even in the absence of inflammation, fluid overload remained a significant risk factor for short-term mortality, even following improvement of fluid status.
We exploit data from the Pan-Andromeda Archaeological Survey (PAndAS) to study the extended structures of M31's dwarf elliptical companions, NGC 147 and NGC 185. Our wide-field, homogeneous ...photometry allows us to construct deep colour–magnitude diagrams which reach down to ∼3 mag below the red giant branch (RGB) tip. We trace the stellar components of the galaxies to surface brightness of μg ∼ 32 mag arcsec−2 and show that they have much larger extents (∼5 kpc radii) than previously recognized. While NGC 185 retains a regular shape in its peripheral regions, NGC 147 exhibits pronounced isophotal twisting due to the emergence of symmetric tidal tails. We fit single Sérsic models to composite surface brightness profiles constructed from diffuse light and star counts and find that NGC 147 has an effective radius almost three times that of NGC 185. In both cases, the effective radii that we calculate are larger by a factor of ∼2 compared to most literature values. We also calculate revised total magnitudes of M
g = −15.36 ± 0.04 for NGC 185 and M
g = −16.36 ± 0.04 for NGC 147. Using photometric metallicities computed for RGB stars, we find NGC 185 to exhibit a metallicity gradient of Fe/H ∼ −0.15 dex kpc−1 over the radial range 0.125–0.5 deg. On the other hand, NGC 147 exhibits almost no metallicity gradient, ∼−0.02 dex kpc−1 from 0.2 to 0.6 deg. The differences in the structure and stellar populations in the outskirts of these systems suggest that tidal influences have played an important role in governing the evolution of NGC 147.
PDF
