dl‐α‐Difluoromethylornithine (F2MeOrn), the most widely‐used inhibitor of l‐ornithine decarboxylase, has been a useful tool to demonstrate that polyamine biosynthesis is required to maintain maximum ...rates of cell proliferation. However, in most eukaryotic cell systems, F2MeOrn exerts cytostatic rather than cytotoxic effects. This may be due to the fact that this inhibitor creates only incomplete polyamine deficiency. In particular, F2MeOrn scarcely depletes intracellular spermine levels.
We now demonstrate in rat hepatoma tissue culture (HTC) cells that (2R, 5R)‐6‐heptyne‐2,5‐diamine, a more potent irreversible inhibitor of l‐ornithine decarboxylase than F2MeOrn, decreases the concentrations of all polyamines including spermine. In parallel with the depletion of these amines, there is a progressive decrease in the rate of cell proliferation and in cell viability. Restoration of the intracellular polyamine content, by addition to the medium of polyamines or a high concentration of l‐ornithine, the substrate of l‐ornithine decarboxylase, further demonstrates that the antiproliferative effects of (2R, 5R)‐6‐heptyne‐2,5‐diamine do result from polyamine deficiency.
These findings support the concept that polyamines play an essential function in the cell division processes and emphasize the vital function of spermine in mammalian cells.
Provider: - Institution: Tallinna Kirjanduskeskus - Data provided by Europeana Collections- sündmuse kommentaar: Ugala teatri suurprojekt A. H. Tammsaare muuseumi õuel Vargamäel.- sündmuse ...kommentaar: Foto Viljandi Draamateatri suurlavastusest "Tõde ja õigus" A. H. Tammsaare muuseumi õuel Vargamäel.- sündmuse kommentaar: A. H. Tammsaare romaani "Tõde ja õigus" I osa (1926) alusel Viljandi Draamateatri "Ugala" ja A. H. Tammsaare Vargamäel asuva muuseumi koostöös valminud suurprojekt, suvelavastus "Tõde ja õigus". Esietendus 21. juunil 1997. aastal. Dramatiseeringu autor Margus Kasterpalu, lavastaja Andres Noormets, kunstnik- kujundaja Anu Raud, muusikaline kujundaja Margus Põldsepp. Osades Hannes Kaljujärv (Andres Paas), Ülle Bernhardt (Krõõt, Liisi), Silvia Sarrap (Maret), Tarvo Vridolin (noor Andres), Piret Rauk (Mari), Meelis Sarv (Indrek), Jaan Rekkor (Pearu Murakas), Anne Valge (Pearu eit), Martin Algus (Joosep), Meelis Rämmeld (Juss) jt. Lavastuses tegid kaasa Viljandi Kultuurikolledži lõpetanud.- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
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