ABSTRACT We present post-cryogenic Spitzer imaging at 3.6 and 4.5 m with the Infrared Array Camera (IRAC) of the Spitzer/HETDEX Exploratory Large-Area (SHELA) survey. SHELA covers 24 deg2 of the ...Sloan Digital Sky Survey "Stripe 82" region, and falls within the footprints of the Hobby-Eberly Telescope Dark Energy Experiment (HETDEX) and the Dark Energy Survey. The HETDEX blind R ∼ 800 spectroscopy will produce ∼200,000 redshifts from the Ly emission for galaxies in the range 1.9 < z < 3.5, and an additional ∼200,000 redshifts from the O ii emission for galaxies at z < 0.5. When combined with deep ugriz images from the Dark Energy Camera, K-band images from NEWFIRM, and other ancillary data, the IRAC photometry from Spitzer will enable a broad range of scientific studies of the relationship between structure formation, galaxy stellar mass, halo mass, the presence of active galactic nuclei, and environment over a co-moving volume of ∼0.5 Gpc3 at 1.9 < z < 3.5. Here, we discuss the properties of the SHELA IRAC data set, including the data acquisition, reduction, validation, and source catalogs. Our tests show that the images and catalogs are 80% (50%) complete to limiting magnitudes of 22.0 (22.6) AB mag in the detection image, which is constructed from the weighted sum of the IRAC 3.6 and 4.5 m images. The catalogs reach limiting sensitivities of 1.1 Jy at both 3.6 and 4.5 m (1 , for R = 2″ circular apertures). As a demonstration of the science, we present IRAC number counts, examples of highly temporally variable sources, and galaxy surface density profiles of rich galaxy clusters. In the spirit of the Spitzer Exploratory programs, we provide all of the images and catalogs as part of the publication.
The distribution of metals within a galaxy traces the baryon cycle and the buildup of galactic disks, but the detailed gas phase metallicity distribution remains poorly sampled. We have determined ...the gas phase oxygen abundances for 7138 H ii regions across the disks of eight nearby galaxies using Very Large Telescope/Multi Unit Spectroscopic Explorer (MUSE) optical integral field spectroscopy as part of the PHANGS-MUSE survey. After removing the first-order radial gradients present in each galaxy, we look at the statistics of the metallicity offset (ΔO/H) and explore azimuthal variations. Across each galaxy, we find low ( = 0.03-0.05 dex) scatter at any given radius, indicative of efficient mixing. We compare physical parameters for those H ii regions that are 1 outliers toward both enhanced and reduced abundances. Regions with enhanced abundances have high ionization parameter, higher H luminosity, lower H velocity dispersion, younger star clusters, and associated molecular gas clouds showing higher molecular gas densities. This indicates recent star formation has locally enriched the material. Regions with reduced abundances show increased H velocity dispersions, suggestive of mixing introducing more pristine material. We observe subtle azimuthal variations in half of the sample, but cannot always cleanly associate this with the spiral pattern. Regions with enhanced and reduced abundances are found distributed throughout the disk, and in half of our galaxies we can identify subsections of spiral arms with clearly associated metallicity gradients. This suggests spiral arms play a role in organizing and mixing the interstellar medium.
MaNGA (Mapping Nearby Galaxies at Apache Point Observatory) is a 6-yr Sloan Digital Sky Survey (SDSS-IV) survey that will obtain spatially resolved spectroscopy from 3600 to 10 300 Å for a ...representative sample of over 10 000 nearby galaxies. In this paper, we present the analysis of nebular emission-line properties using observations of 14 galaxies obtained with P-MaNGA, a prototype of the MaNGA instrument. By using spatially resolved diagnostic diagrams, we find extended star formation in galaxies that are centrally dominated by Seyfert/LINER-like emission, which illustrates that galaxy characterizations based on single fibre spectra are necessarily incomplete. We observe extended low ionization nuclear emission-line regions (LINER)-like emission (up to 1R
e) in the central regions of three galaxies. We make use of the Hα equivalent width EW(Hα) to argue that the observed emission is consistent with ionization from hot evolved stars. We derive stellar population indices and demonstrate a clear correlation between D
n(4000) and EW(HδA) and the position in the ionization diagnostic diagram: resolved galactic regions which are ionized by a Seyfert/LINER-like radiation field are also devoid of recent star formation and host older and/or more metal-rich stellar populations. We also detect extraplanar LINER-like emission in two highly inclined galaxies, and identify it with diffuse ionized gas. We investigate spatially resolved metallicities and find a positive correlation between metallicity and star formation rate surface density. We further study the relation between N/O versus O/H on resolved scales. We find that, at given N/O, regions within individual galaxies are spread towards lower metallicities, deviating from the sequence defined by galactic central regions as traced by Sloan 3-arcsec fibre spectra. We suggest that the observed dispersion can be a tracer for gas flows in galaxies: infalls of pristine gas and/or the effect of a galactic fountain.
We use integral field spectroscopy from the PHANGS–MUSE survey, which resolves the ionised interstellar medium structure at ∼50 pc resolution in 19 nearby spiral galaxies, to study the origin of the ...diffuse ionised gas (DIG). We examine the physical conditions of the diffuse gas by first removing morphologically defined H
II
regions and then binning the low-surface-brightness areas to achieve significant detections of the key nebular lines in the DIG. A simple model for the leakage and propagation of ionising radiation from H
II
regions is able to reproduce the observed distribution of H
α
in the DIG. This model infers a typical mean free path for the ionising radiation of 1.9 kpc for photons propagating within the disc plane. Leaking radiation from H
II
regions also explains the observed decrease in line ratios of low-ionisation species (S
II
/H
α
, N
II
/H
α
, and O
I
/H
α
) with increasing H
α
surface brightness (Σ
H
α
). Emission from hot low-mass evolved stars, however, is required to explain: (1) the enhanced low-ionisation line ratios observed in the central regions of some of the galaxies in our sample; (2) the observed trends of a flat or decreasing O
III
/H
β
with Σ
H
α
; and (3) the offset of some DIG regions from the typical locus of H
II
regions in the Baldwin–Phillips–Terlevich (BPT) diagram, extending into the area of low-ionisation (nuclear) emission-line regions (LINERs). Hot low-mass evolved stars make a small contribution to the energy budget of the DIG (2% of the galaxy-integrated H
α
emission), but their harder spectra make them fundamental contributors to O
III
emission. The DIG might result from a superposition of two components, an energetically dominant contribution from young stars and a more diffuse background of harder ionising photons from old stars. This unified framework bridges observations of the Milky Way DIG with LI(N)ER-like emission observed in nearby galaxy bulges.
Phylogenetic analyses are central to many research areas in biology and typically involve the identification of homologous sequences, their multiple alignment, the phylogenetic reconstruction and the ...graphical representation of the inferred tree. The Phylogeny.fr platform transparently chains programs to automatically perform these tasks. It is primarily designed for biologists with no experience in phylogeny, but can also meet the needs of specialists; the first ones will find up-to-date tools chained in a phylogeny pipeline to analyze their data in a simple and robust way, while the specialists will be able to easily build and run sophisticated analyses. Phylogeny.fr offers three main modes. The 'One Click' mode targets non-specialists and provides a ready-to-use pipeline chaining programs with recognized accuracy and speed: MUSCLE for multiple alignment, PhyML for tree building, and TreeDyn for tree rendering. All parameters are set up to suit most studies, and users only have to provide their input sequences to obtain a ready-to-print tree. The 'Advanced' mode uses the same pipeline but allows the parameters of each program to be customized by users. The 'A la Carte' mode offers more flexibility and sophistication, as users can build their own pipeline by selecting and setting up the required steps from a large choice of tools to suit their specific needs. Prior to phylogenetic analysis, users can also collect neighbors of a query sequence by running BLAST on general or specialized databases. A guide tree then helps to select neighbor sequences to be used as input for the phylogeny pipeline. Phylogeny.fr is available at: http://www.phylogeny.fr/
Studying how photosynthetic cells modify membrane lipids in response to heat stress is important to understand how plants and microalgae adapt to daily fluctuations in temperature and to investigate ...new lipid pathways. Here, we investigate changes occurring in lipid molecular species and lipid metabolism genes during early response to heat stress in the model photosynthetic microorganism Chlamydomonas reinhardtii. Lipid molecular species analyses revealed that, after 60 min at 42 °C, a strong decrease in specific polyunsaturated membrane lipids was observed together with an increase in polyunsaturated triacylglycerols (TAGs) and diacylglycerols (DAGs). The fact that decrease in the major chloroplastic monogalactosyldiacylglycerol sn1‐18:3/sn2‐16:4 was mirrored by an accumulation of DAG sn1‐18:3/sn2‐16:4 and TAG sn1‐18:3/sn2‐16:4/sn3‐18:3 indicated that newly accumulated TAGs were formed via direct conversion of monogalactosyldiacylglycerols to DAGs then TAGs. Lipidomic analyses showed that the third fatty acid of a TAG likely originated from a phosphatidylethanolamine or a diacylglyceryl‐O‐4′‐(N,N,N,‐trimethyl)‐homoserine betaine lipid species. Candidate genes for this TAG synthesis pathway were provided through comparative transcriptomic analysis and included a phospholipase A2 homolog and the DAG acyltransferase DGTT1. This study gives insights into the molecular events underlying changes in membrane lipids during heat stress and reveals an alternative route for TAG synthesis.
In the model photosynthetic organism Chlamydomonas reinhardtii, we show that the early response of plastidial membranes to heat stress involves selective reduction of polyunsaturated monogalactosylglycerol species by conversion to diacylglycerols and triacylglycerols. Candidate genes responsible for this conversion are identified by comparative transcriptomics of heat‐stressed to control cells.
ABSTRACT
The feedback from young stars (i.e. pre-supernova) is thought to play a crucial role in molecular cloud destruction. In this paper, we assess the feedback mechanisms acting within a sample ...of 5810 H ii regions identified from the PHANGS-MUSE survey of 19 nearby (<20 Mpc) star-forming, main-sequence spiral galaxies log(M⋆/M⊙) = 9.4–11. These optical spectroscopic maps are essential to constrain the physical properties of the H ii regions, which we use to investigate their internal pressure terms. We estimate the photoionized gas (Ptherm), direct radiation (Prad), and mechanical wind pressure (Pwind), which we compare to the confining pressure of their host environment (Pde). The H ii regions remain unresolved within our ∼50–100 pc resolution observations, so we place upper (Pmax) and lower (Pmin) limits on each of the pressures by using a minimum (i.e. clumpy structure) and maximum (i.e. smooth structure) size, respectively. We find that the Pmax measurements are broadly similar, and for Pmin the Ptherm is mildly dominant. We find that the majority of H ii regions are overpressured, Ptot/Pde = (Ptherm + Pwind + Prad)/Pde > 1, and expanding, yet there is a small sample of compact H ii regions with Ptot,max/Pde < 1 (∼1 per cent of the sample). These mostly reside in galaxy centres (Rgal < 1 kpc), or, specifically, environments of high gas surface density; log(Σgas/M⊙ pc−2) ∼ 2.5 (measured on kpc-scales). Lastly, we compare to a sample of literature measurements for Ptherm and Prad to investigate how dominant pressure term transitions over around 5 dex in spatial dynamic range and 10 dex in pressure.
Quantitative trait loci (QTL) detection experiments have often been restricted to large biallelic populations. Use of connected multiparental crosses has been proposed to increase the genetic ...variability addressed and to test for epistatic interactions between QTL and the genetic background. We present here the results of a QTL detection performed on six connected F₂ populations of 150 F₂:₃ families each, derived from four maize inbreds and evaluated for three traits of agronomic interest. The QTL detection was carried out by composite interval mapping on each population separately, then on the global design either by taking into account the connections between populations or not. Epistatic interactions between loci and with the genetic background were tested. Taking into account the connections between populations increased the number of QTL detected and the accuracy of QTL position estimates. We detected many epistatic interactions, particularly for grain yield QTL (R ² increase of 9.6%). Use of connections for the QTL detection also allowed a global ranking of alleles at each QTL. Allelic relationships and epistasis both contribute to the lack of consistency for QTL positions observed among populations, in addition to the limited power of the tests. The potential benefit of assembling favorable alleles by marker-assisted selection are discussed.
Zn isotopes were used to trace the anthropogenic sources in the Lot watershed (Aveyron, SW France) where a small river, the Riou Mort drains an industrial exploitation of zinc ore. The zinc isotopes ...in industrial tailings are highly fractionated relatively to Zn ore (
δ
66Zn
∼
0.16‰), due to metallurgical processes, reaching
δ
66Zn values up to +
1.49‰. Zn extraction yields for these samples were calculated using presumable conservative elements (Fe, Ca, Mg, K, Na and Mn) and vary from 95.4% to 99.4%. Extraction yields are related to the intensity of Zn isotopic fractionation. Different Rayleigh isotope fractionation scenarios for Zn refining yielded
α
ore-tailings ranging from 1.00012 to 1.00062. The
δ
66Zn of the Riou Mort sediments downstream from the former Zn-ore facility is presently +
0.91
±
0.04‰, i.e. significantly different from the signature of upstream sediments and of the regional geochemical background, +
0.31
±
0.06‰, (2SD,
n
=
3). Sediment core
δ
66Zn values in the 40 km downstream Cajarc hydroelectric reservoir are also consistently ‘heavier’ (+
0.75 to +
1.35‰) than the regional geochemical background. Both top-core Zn concentrations and isotopic signatures can be explained by the contribution of 9% of present-day Riou Mort sediments.
δ
66Zn variations in the
137Cs-dated downcore sediments are suggested to reflect historical changes in metallurgical processes of the Viviez facility, such as improved Zn extraction efficiency and the related evolution of tailings. In sediments deposited during 1952–1972, the mean
δ
66Zn is +
0.95
±
0.08‰, representative of the combined electrolysis and thermal process used at that time for the Zn extraction. In sediments deposited during the late seventies,
δ
66Zn increases, reaching a maximum of 1.35‰ during the eighties. These heavier
δ
66Zn are likely related to further increased Zn extraction after replacement of thermal waste processing by electrolysis in 1976/77. The nature of the extraction processes, especially electrolysis, may therefore play a major role in the Zn isotopic fractionation produced. The soils located on the Lot riverbanks have chemical and Zn isotopic compositions (+
0.74‰ to +
0.96‰) close to the Cajarc sediments, indicating that they were also impacted by tailings erosion and leaching. The soils located in the vicinity of the plant display lower
δ
66Zn (+
0.40‰ to 0.48‰). That corresponds to mixtures of several atmospheric sources like coal fly ashes, ore and tailing dusts. The relation between Zn isotopic fractionation, extraction yields and/or extraction processes clearly demonstrates the efficiency of
δ
66Zn as tracer of environmental contamination.
Platinum Group Metals (PGM) are modern, technologically relevant elements for which (i) the anthropogenic cycle has outcompeted the natural cycles and (ii) environmental behavior, fate and impact are ...still widely unknown. Stripping voltammetry was used for accurate determinations of platinum (Pt) in historical records of river sediments and estuarine oysters from the Gironde fluvial–estuarine continuum (SW France) comprising the Lot River. Sediment cores from the Lot River, dated from 1952 to 2001, showed past Pt contamination due to former industrial (smelter) activities in the Lot River watershed. These samples revealed the phasing-out of a historical Pt contamination with Pt/Th (Thorium) values of 11×10−5±0.79×10−5 for the deepest part of the core which is clearly greater than the regional geochemical background value (Pt/Th ~2.2×10−5±0.68×10−5). Wild oyster samples from the mouth of the Gironde Estuary collected from 1981 to 2013 showed Pt concentrations ranging from 0.80±0.01pmol.g−1 to 3.10±0.14pmol.g−1. Oyster samples have recorded the phasing-out of the smelter-related historical industrial Pt contamination and empirical modeling suggests the recent rise of a new source of Pt to the system. Temporal variations of Pt in oysters attributed to this recent source reflect the exponential increase of Pt demand for car catalytic converters, pointing towards the increasing importance of this emerging source to the aquatic system. Estuarine oysters prove to be suitable bioindicators for Pt contamination providing sensitive monitoring of emission variations over time. Furthermore, oysters may bioconcentrate Pt (bioconcentration factor, BCF ~103) and transfer this metal contamination to the higher food chain. These findings highlight the need for a deeper understanding of environmental Pt contamination, processes and possible adverse effects to biota.
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•Historical Pt records reflect past and present anthropogenic sources.•Wild oysters are sensitive biomonitors of Pt contamination that bioconcentrate Pt.•Anthropogenic Pt emissions may profoundly affect Pt budget of the watershed.