Vodonosnik ob Mislinji zaradi majhne izdatnosti in dejstva, da se ne uporablja za vodooskrbo, ni vključen v redni državni monitoring kakovosti podzemnih voda. V članku na primeru devetih vzorčnih ...mest (zasebnih vodnjakov in vrtin) analiziramo vrednosti osnovnih fizikalno-kemijskih parametrov vode, na podlagi katerih preliminarno ocenjujemo njeno kakovost. Ugotavljamo, da se v podtalnici odražajo antropogeni vplivi, ki so izrazitejši v bližini območij strnjene kmetijske obdelave ter ob večjih naseljih. Pri večini vzorcev so prisotne povišane vrednosti nitratov in kloridov, pri nekaterih pa se v višjih koncentracijah pojavljajo tudi druga merjena onesnažila. Glede na aktualni Pravilnik o pitni vodi je kar pet od devetih vzorcev neustreznih, oziroma zgolj pogojno ustreznih, kar pomeni, da je podtalnica v vodonosniku ob Mislinji morda že preveč onesnažena, da bi nanjo lahko računali kot na možni rezervni vodni vir za vodooskrbo.
Studying karst water dynamics is challenging because of the often unknown underground flows. Therefore, studies of visible karst waters receive considerable research emphasis. Researchers are turning ...to various data sources, including remote sensing imagery, to study them. This research paper presents an assessment of a water bodies dataset, automatically detected from Sentinel-1 imagery, for karst flood research. Statistical and visual analyses were conducted to assess the reliability and effectiveness of the dataset. Spearman’s correlation coefficients were employed for statistical analysis to determine the degree of correlation between the areas of water bodies dataset and official water level data. Visual analyses involved the creation of heat maps based on the identified water areas, which were then compared to official flood maps, and the preparation of an analysis of historical flood events or results of hydrological and hydraulic modelling. Additionally, vegetation maps were produced to identify areas that lacked detection and complemented the heat maps. Statistical assessment showed a strong correlation (≥0.6) between the dataset and official water level data in smaller flood-prone areas with less complex inflow. Visual analyses using heat maps and vegetation maps effectively identified frequently flooded areas but had limitations in areas with dense vegetation. Comparisons with flood maps showed an important value of the dataset as an additional source of information for karst flood studies. This assessment highlights the dataset’s potential in combination with other data sources and modelling approaches.
Demografski podatki in podatki o razpoložljivosti kapacitet domov starejših občanov (DSO) v Sloveniji nakazujejo potrebo po spremembi organiziranosti tega segmenta varstva. V prispevku smo se ...osredotočili na analizo in vrednotenje stanja urejenosti in dostopnosti zelenih površin ter storitev splošnega pomena v majhnih mestih z vidika potreb starejših prebivalcev, ki svojo starost preživljajo v DSO. Hkrati smo ocenili možnosti za dejansko uporabo zelenih površin te skupine prebivalstva. Ugotovili smo, da v Sloveniji potrebujemo temeljite sistemske spremembe na področju institucionalnega varstva starejših občanov, tudi na področju zagotavljanja, urejenosti in uporabe zelenih površin ob DSO.
Work at home and work from home are becoming the subject of interdisciplinary research in the current social conditions. Slovenia, as a post-transition country, has specific experience in terms of ...its regulation, as the former socialist and later transition period were relatively tolerant of various forms of work at home. The article presents the results of research aimed at studying current normative provisions for the organization of work at home, its actual spatial and program scope, and its correlation with building typology and morphology. Using a descriptive research method and by analyzing existing databases in the GIS environment, we found that work at home is a very extensive phenomenon in Slovenia. Despite the effective instruments in the fields of spatial planning, public administration, tax system, and employment legal relationships, its scope is mainly a consequence of historical tolerance, as this form of work has been legally organized and desirable for decades. We found that various urban characteristics did not significantly affect its occurrence in the past. The differences are reflected only in the extent of business activities that can be carried out in residential areas and differ according to the distance from urban centers. In order for the regulation of work at home to become even more efficient in the future, it is necessary to define more detailed criteria, especially in terms of its program regulation and monitoring of the spatial situation.
The problem of the currently used routine genotoxicity tests is relatively low predictivity of in vitro tests for in vivo genotoxicity and carcinogenicity. An important reason is considered to be ...inadequate expression of xenobiotic-metabolizing enzymes in indicator cell lines. The aim of our study was to generate metabolically active differentiated hepatic progenies (hDHP) from human adipose tissue-derived mesenchymal stem cells (hASC) for genotoxicity testing. hDHP, generated using a three-step hepatic differentiation procedure, expressed hepatic properties such as glycogen storage and albumin secretion. The results of the comet assay demonstrated comparable sensitivity of hASC and hDHP to detect DNA damage induced by a direct acting genotoxic agent tert-butylhydroperoxide. Exposure to model indirect acting genotoxins benzo(a)pyrene, aflatoxin B
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, and 2-amino-1-methyl-6-phenylimidazo4,5-bpyridine did not induce DNA damage in hASC, while hDHP cells detected DNA damage induced by benzo(a)pyrene and aflatoxin B
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, indicating their metabolic activity. The gene and protein expression analysis confirmed the presence of key enzymes involved in metabolism of the three genotoxins in hDHP cells. Moreover, the exposure of hDHP to the model pro-carcinogens altered the expression of selected metabolic genes. hDHP were further immortalized with hTERT transfection, resulting in a stable cell line that can be matured to metabolically active hDHP ready for genotoxicity testing in only 2 weeks. The advantage of these immortalized cells is their prolonged replicative life span and consequently limitless supply of hDHP cells. We conclude that hDHP cells have a great potential for the application in the routine genotoxicity testing and are therefore worth further investigations.
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