Eicosapentaenoic acid (EPA), a n-3 long-chain polyunsaturated fatty acid, has been reported to have beneficial effects in obesity-associated metabolic disorders. The objective of the present study ...was to determine the effects of EPA on the regulation of genes involved in lipid metabolism, and the ability of EPA to induce mitochondrial biogenesis and beiging in subcutaneous adipocytes from overweight subjects. Fully differentiated human subcutaneous adipocytes from overweight females (BMI: 28.1–29.8kg/m2) were treated with EPA (100–200 μM) for 24 h. Changes in mRNA expression levels of genes involved in lipogenesis, fatty acid oxidation and mitochondrial biogenesis were determined by qRT-PCR. Mitochondrial content was evaluated using MitoTracker® Green stain. The effects on peroxisome proliferator-activated receptor gamma, co-activator 1 alpha (PGC-1α) and AMP-activated protein kinase (AMPK) were also characterized. EPA down-regulated lipogenic genes expression while up-regulated genes involved in fatty acid oxidation. Moreover, EPA-treated adipocytes showed increased mitochondrial content, accompanied by an up-regulation of nuclear respiratory factor-1, mitochondrial transcription factor A and cytochrome c oxidase IV mRNA expression. EPA also promoted the activation of master regulators of mitochondrial biogenesis such as sirtuin 1, PGC1-α and AMPK. In parallel, EPA induced the expression of genes that typify beige adipocytes such as fat determination factor PR domain containing 16, uncoupling protein 1 and cell death-inducing DFFA-like effector A, T-Box protein 1 and CD137. Our results suggest that EPA induces a remodeling of adipocyte metabolism preventing fat storage and promoting fatty acid oxidation, mitochondrial biogenesis and beige-like markers in human subcutaneous adipocytes from overweight subjects.
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Abstract
We report the results of 6 years (2013–2018) of measurements of
$$^{222}$$
222
Rn air concentration, relative humidity, atmospheric pressure and temperature in the halls A, B and C of the ...Canfranc Underground Laboratory (LSC). We have calculated all the Pearson correlation coefficients among these parameters and we have found a positive correlation between the
$$^{222}$$
222
Rn concentration and the relative humidity. Both correlated variables show a seasonal periodicity. The joint analysis of laboratory data and 4 years (2015–2018) of the meteorological variables outside the laboratory shows the correlation between the
$$^{222}$$
222
Rn concentration and the outside temperature. The collected information stresses the relevance of designing good Rn-mitigation strategies in current and future experiments at LSC; in particular, we have checked for two years (2017–2018) the good performance of the mitigation procedure of the ANAIS-112 experiment. Finally, we have monitored (2019–2021) for 2 years of live time, the radon-free air provided by the radon abatement system installed in the laboratory.
The first activities of the Canfranc Underground Laboratory (“Laboratorio Subterráneo de Canfanc”, LSC) started in the mid-eighties in a railway tunnel located under the Spanish Pyrenees; since then, ...it has become an international multidisciplinary facility equipped with different services for underground science. The research activity at LSC is about Astroparticle Physics, dark matter searches and neutrino Physics; but also activities in Nuclear Astrophysics, Geophysics, and Biology are carried out. The investigation of the neutrinoless double beta decay has been one of the main research lines of LSC since the beginning. Many unknowns remain in the characterization of the basic neutrino properties and the study of this rare decay process requiring Physics beyond the Standard Model of Particle Physics can shed light on the lepton number conservation, the nature of the neutrinos as Dirac or Majorana particles and the absolute scale and ordering of the masses of the three generations. Here, the double beta decay searches performed at LSC for different emitters and following very different experimental approaches will be reviewed: from the very first experiments in the laboratory including the successful IGEX (“International Germanium EXperiment”) for 76Ge, which released very stringent limits to the effective neutrino mass at the time, to the present NEXT experiment (“Neutrino Experiment with a Xenon Time-Projection Chamber”) for136Xe and future project CROSS (“Cryogenic Rare-event Observatory with Surface Sensitivity”) for130Te and100Mo, both implementing innovative detector technologies to discriminate backgrounds. For the neutrinoless double beta decay channel and at 90% C.L., IGEX derived a limit to the half-life of76Ge ofT1∕20ν>1.57×1025 y while the corresponding expected limits are T1∕20ν>1.0×1026 y for136Xe from NEXT-100 (for an exposure of 500 kg⋅y) and T1∕20ν>2.8×1025 y for100Mo from CROSS (for 5 y and 4.7 kg of isotope). Activities related to double beta decays searches carried out in other underground laboratories have also been developed at LSC and will be presented too, like the operation of the BiPo-3 detector for radiopurity measurements of thin sheets with very high sensitivity. For each one of these experiments, the concept, the experimental set-ups and relevant results will be discussed.
We report the results of 6 years (2013–2018) of measurements of
222
Rn air concentration, relative humidity, atmospheric pressure and temperature in the halls A, B and C of the Canfranc Underground ...Laboratory (LSC). We have calculated all the Pearson correlation coefficients among these parameters and we have found a positive correlation between the
222
Rn concentration and the relative humidity. Both correlated variables show a seasonal periodicity. The joint analysis of laboratory data and 4 years (2015–2018) of the meteorological variables outside the laboratory shows the correlation between the
222
Rn concentration and the outside temperature. The collected information stresses the relevance of designing good Rn-mitigation strategies in current and future experiments at LSC; in particular, we have checked for two years (2017–2018) the good performance of the mitigation procedure of the ANAIS-112 experiment. Finally, we have monitored (2019–2021) for 2 years of live time, the radon-free air provided by the radon abatement system installed in the laboratory.
Abstract
ANAIS is a direct dark matter detection experiment aiming at the confirmation or refutation of the DAMA/LIBRA positive annual modulation signal in the low energy detection rate, using the ...same target and technique. ANAIS–112, located at the Canfranc Underground Laboratory in Spain, is operating an array of 3×3 ultrapure NaI(Tl) crystals with a total mass of 112.5 kg since August 2017. The trigger rate in the region of interest (1-6 keV) is dominated by non-bulk scintillation events. In order to discriminate these noise events from bulk scintillation events, robust filtering protocols have been developed. Although this filtering procedure works very well above 2 keV, the measured rate from 1 to 2 keV is about 50% higher than expected according to our background model, and we cannot discard non-bulk scintillation events as responsible of that excess. In order to improve the rejection of noise events, a Boosted Decision Tree has been developed and applied. With this new PMT-related noise rejection algorithm, the ANAIS–112 background between 1 and 2 keV is reduced by almost 30%, leading to an increase in sensitivity to the annual modulation signal. The reanalysis of the three years of ANAIS–112 data with this technique is also presented.
The ANAIS (Annual modulation with NaI(Tl) Scintillators) experiment aims at the confirmation or refutation of the DAMA/LIBRA positive annual modulation signal in the low energy detection rate, using ...the same target and technique, at the Canfranc Underground Laboratory (LSC) in Spain. ANAIS-112, consisting of nine 12.5 kg NaI(Tl) modules produced by Alpha Spectra Inc. in a
3
×
3
matrix configuration, is taking data smoothly in “dark matter search” mode since August, 2017, after a commissioning phase and operation of the first detectors during the last years in various set-ups. A large effort has been carried out within ANAIS to characterize the background of sodium iodide detectors, before unblinding the data and performing the first annual modulation analysis. Here, the background models developed for all the nine ANAIS-112 detectors are presented. Measured spectra from threshold to high energy in different conditions are well described by the models based on quantified activities independently estimated following several approaches. In the region from 1 to
6
keV
ee
the measured, efficiency corrected background level is
3.58
±
0.02
keV
-
1
kg
-
1
day
-
1
; NaI crystal bulk contamination is the dominant background source being
210
Pb
,
40
K
,
22
Na
and
3
H
contributions the most relevant ones. This background level, added to the achieved
1
keV
ee
analysis threshold (thanks to the outstanding light collection and robust filtering procedures developed), allow ANAIS-112 to be sensitive to the modulation amplitude measured by DAMA/LIBRA, and able to explore at three sigma level in 5 years the WIMP parameter region singled out by this experiment.
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