Tourism is a beautiful thing, tourism can bring tourists a variety of enjoyment and experience, let tourists harvest instant joy and happiness, leave them with good impressions and memories, so that ...tourists are relaxed and soothed, so tourism is actually a good way of life. As a tourism industry that has developed rapidly after the epidemic in China, “micro-vacation” has increasingly become the first choice for people’s daily travel. This paper combines the customer positioning case of Aranya Tourism Resort in China, analyzes the characteristics of micro-vacation customers through the professional knowledge of marketing management such as market positioning and market segmentation, and explains how to do the micro-vacation customer group positioning analysis of tourism projects.
The role of p53 in renal fibrosis has recently been suggested, however, its function remains controversial and the underlying mechanism is unclear. Here, we show that pharmacological and genetic ...blockade of p53 attenuated renal interstitial fibrosis, apoptosis, and inflammation in mice with unilateral urethral obstruction (UUO). Interestingly, p53 blockade was associated with the suppression of miR-215-5p, miR-199a-5p&3p, and STAT3. In cultured human kidney tubular epithelial cells (HK-2), TGF-β1 treatment induced fibrotic changes, including collagen I and vimentin expression, being associated with p53 accumulation, p53 Ser15 phosphorylation, and miR-199a-3p expression. Inhibition of p53 by pifithrin-α blocked STAT3 activation and the expression of miR-199a-3p, collagen I, and vimentin during TGF-β1 treatment. Over-expression of miR-199a-3p increased TGFβ1-induced collagen I and vimentin expression and restored SOCS7 expression. Furthermore, SOCS7 was identified as a target gene of miR-199a-3p, and silencing of SOCS7 promoted STAT3 activation. ChIp analyses indicated the binding of p53 to the promoter region of miR-199a-3p. Consistently, kidney biopsies from patients with IgA nephropathy and diabetic nephropathy exhibited substantial activation of p53 and STAT3, decreased expression of SOCS7, and increase in profibrotic proteins and miR-199a-3p. Together, these results demonstrate the novel p53/miR-199a-3p/SOCS7/STAT3 pathway in renal interstitial fibrosis.
Mycophenolic acid (MPA)-induced colitis was still a severe side effect and challenge faced by solid transplant recipients. We aimed to explore the function and mechanism of probiotics in the ...treatment of MPA-induced colitis.
In this study, 15 mice (C57BL/6) were randomly assigned into three groups: control (CNTL) group (
= 5), MPA group (
= 5) and the MPA + Probiotic group (
= 5). Bifid Triple Viable capsules, which were drugs for clinical use and consisted of
,
, and
, were used in Probiotic group. Body weight change, stool scores, colon histopathology and morphology were used to evaluate the disease severity. The intestinal mucosal barrier function was assessed by measuring the expression level of secretory immunoglobulin A (sIgA), Zonula occludens-1 (ZO-1) and Occludin. Finally, 16S rDNA sequencing and bioinformatics analysis were performed on mice feces to compare the different intestinal microbial composition and diversity among three groups.
Compared with the CNTL group, the mice in MPA group showed a significantly decreased body weight, increased stool scores, shortened colon length and severe colon inflammation. However, probiotics treated MPA mice reversed the disease severity, indicating that probiotics ameliorated MPA-induced colitis in mice. Mechanistically, probiotics improved the intestinal barrier function by up-regulating the expression of sIgA, ZO-1 and Occludin. Moreover, MPA-induced colitis led to intestinal microbiota dysbiosis, including the change of
/
ratio, α- and β-diversity. But probiotic treated group showed mild change in these microbial features. Additionally, we found that
was the most significantly different microbiota flora in MPA group.
Probiotics treatment ameliorated MPA-induced colitis by enhancing intestinal barrier function and improving intestinal microbiota dysbiosis.
might be the dominant functional intestinal microflora and serve as the potential therapy target in MPA-induced colitis.
Emerging evidence indicates that long noncoding RNAs (lncRNAs) regulate various biological processes, especially innate and adaptive immunity. However, the relationship between lncRNAs and the ...interferon (IFN) pathway remains largely unknown. Here, we report that lncRNA ITPRIP-1 (lncITPRIP-1) is involved in viral infection and plays a crucial role in the virus-triggered IFN signaling pathway through the targeting of melanoma differentiation-associated gene 5 (MDA5). LncITPRIP-1 can be induced by viral infection, which is not entirely dependent on the IFN signal. Besides, there is no coding potential found in the lncITPRIP-1 transcript. LncITPRIP-1 binds to the C terminus of MDA5, and it possesses the ability to boost the oligomerization of both the full length and the 2 caspase activation and recruitment domains of MDA5 in a K63-linked polyubiquitination-independent manner. Amazingly, we also found that MDA5 can suppress hepatitis C virus (HCV) replication independently of IFN signaling through its C-terminal-deficient domain bound to viral RNA, in which lncITPRIP-1 plays a role as an assistant. In addition, the expression of lncITPRIP-1 is highly consistent with MDA5 expression, indicating that lncITPRIP-1 may function as a cofactor of MDA5. All the data suggest that lncITPRIP-1 enhances the innate immune response to viral infection through the promotion of oligomerization and activation of MDA5. Our study discovers the first lncRNA ITPRIP-1 involved in MDA5 activation.
Hepatitis C virus infection is a global health issue, and there is still no available vaccine, which makes it urgent to reveal the underlying mechanisms of HCV and host factors. Although RIG-I has been recognized as the leading cytoplasmic sensor against HCV for a long time, recent findings that MDA5 regulates the IFN response to HCV have emerged. Our work validates the significant role of MDA5 in IFN signaling and HCV infection and proposes the first lncRNA inhibiting HCV replication by promoting the activation of MDA5 and mediating the association between MDA5 and HCV RNA, the study of which may shed light on the MDA5 function and treatment for hepatitis C patients. Our suggested model of how lncITPRIP-1 orchestrates signal transduction for IFN production illustrates the essential role of lncRNAs in virus elimination.
Characterization of the chemical composition of petroleum vacuum distillate fractions is essential to advance the understanding of the fundamental chemistry of refining processes, such as fluid ...catalytic cracking and hydrocracking. This is a challenging task, primarily as a result of the limitations of current analytical techniques to deal with heavy hydrocarbon samples. A different path toward this goal is through the use of hydrocarbon composition modeling techniques to derive the molecular make up of petroleum fractions with limited analytical data. The purpose of this study is to demonstrate this approach for simulating the molecular composition of vacuum distillates. The method consists of generating a computational mixture of representative hydrocarbon molecules that mimics the properties of an actual oil sample. Molecules are built according to the specific chemistry of vacuum distillates with a Monte Carlo algorithm, and the abundance of each molecule is optimized by entropy maximization. The model was applied to simulate two vacuum gas oil samples differing substantially in chemical composition and geographic origin. The samples were experimentally characterized in detail to obtain the necessary model inputs. Simulations revealed that the model adequately predicts the analytical properties and carbon number distributions of the two samples, proving its capability to capture a wide range of distinct vacuum distillate chemistries.
CFD simulations of gas–liquid–solid fluidized beds have been performed in a full three-dimensional, unsteady multiple-Euler framework by means of the commercial software FLUENT. The simulation ...results were compared with experimental data obtained from laboratory scale three-phase fluidized beds. The significance of implementing accurate numerical schemes, as well as the choice of available k–ε turbulence models (standard, RNG, realizable), solid wall boundary conditions and granular temperature models were investigated. The results indicate that in order to minimize numerical diffusion artifacts and to enable valid discussions on the choice of physical models, third-order numerical schemes need to be implemented. The realizable turbulence formulation was unable to produce the expected solids gulf-stream pattern (i.e., rising solid particles in the core and descending solid particles near the wall) in three-phase fluidized beds whereas the RNG and standard k–ε models were able to better capture depictions of flow patterns. The best prediction of flow characteristics was obtained with a laminar model formulation accounting for the solid phase viscosity and the molecular viscosities of the two fluids.
► CFD simulation of three-phase fluidized bed hydrodynamics. ► Systematic implementation of phasic k–ε turbulence models. ► Laminar versus turbulence models. ► Measured axial solid velocity and gas holdup best predicted by laminar model.
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•HMTP was isolated from the green tea of Huangshan Maofeng.•HMTP has strong free radicals-scavenging and antioxidant capacities in vitro.•HMTP was appraised as a hepatoprotective ...agent against CCl4-induced damage in mice.•Pathological test confirmed the protection on CCl4-caused histological alteration.•HMTP has potential to be explored as a valuable hepatoprotective function food.
The study was to characterize the polyphenolic composition, antioxidant properties, and hepatoprotective effects of a polyphenols-enriched extract (HMTP) from Huangshan Maofeng green tea. HPLC analysis showed that three predominantly polyphenolic compounds present in HMTP were epigallocatechin (271.2μg/mg extract), rutin (239.3μg/mg) and epicatechin (89.3μg/mg). HMTP was shown to exhibit strong scavenging activities against DPPH, O2−, and OH, and ferric-reducing antioxidant power in vitro. Administration of HMTP at 200, 400 and 800mg/kgbw in mice prior to CCl4 injury significantly decreased the CCl4-induced elevation of serum ALT, AST and ALP activities, and prevented an increase in hepatic MDA levels (p<0.05). Mice with HMTP pretreatment displayed a better profile of hepatosomatic index and the improved GSH-Px and SOD activities in the liver, relative to CCl4-intoxicated mice. Liver pathological observation also confirmed the protection on CCl4-caused histological alteration, suggesting that HMTP has potential to be explored as valuable hepatoprotective function food.
Modeling and Control of Data Transmission Tanhao, Huang; Siqi, Jian; Jinwen, Chen ...
Methodology and computing in applied probability,
09/2023, Volume:
25, Issue:
3
Journal Article
Peer reviewed
This paper concerns a class of stochastic recursive systems which we treat as stochastic decision models. Problems for optimal control are considered via the limiting path of the system. The ...existence of such a limiting path is provided by a law of large numbers type theorem. This limiting path is shown to be the integral path of a differential equation closely related to the dynamics of the recursive system. Examples of simulation for convergence are provided. Applications to a simple model of data transmission and a simple model of high frequency trading with optimal strategies are discussed.
In recent years, pushed particularly by COVID-19 restrictions and lockdowns, virtual photovoice studies have emerged as an alternative modality of photovoice. Alongside this trend are questions about ...virtual photovoice practice and methodological questions of participation. This paper interrogates these questions using an Australian photovoice research with LGBTQ+ multicultural older people. Threading through the photovoice stages of conceptualisation, recruitment, photovoice production and discussions, it discusses participation in relation to both in-person and virtual photovoice literatures, highlights challenges and new opportunities. Reflecting on virtual photovoice as a method and a methodology, it positions virtual photovoice as a comparable choice that researchers can make.
Objectives: With the rise of age-friendly cities and communities, increasing attention is being paid to coproducing age-friendly guidelines with older people and community stakeholders. Little is ...known however about age-friendly guidelines for dining establishments.
Methods: A three-stage study to develop general and contextualised requirements for an age-friendly dining experience was conducted in the City of Onkaparinga, South Australia. The first stage involved older people in co-designing aspects of an age-friendly dining experience. Subsequently, the second and third stage coproduced, trialed and evaluated age-friendly initiatives with two dining venues.
Results: Through co-design, seven domains of an age-friendly dining experience were identified (Menu, Affordability, Dementia Awareness, Venue, Feeling Welcome, Special Offerings and Assistance), alongside an overarching desire for a 'meaningful' dining experience. Differences in health and socioeconomic status of diners underpinned the differences needed to ensure an age-friendly dining experience and highlight the importance of contextualisation for the local population. The trial demonstrated positive outcomes for both older diners and venues.
Discussion: The environment, value and logistics of dining out are important to older people when making choices about dining in the community. The elements of an age-friendly dining experience presented in this study are a useful starting point for contextualisation to other local settings.
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