The design of the MEG II experiment Baldini, A. M; Baracchini, E; Bemporad, C ...
The European physical journal. C, Particles and fields,
05/2018, Volume:
78, Issue:
5
Journal Article
Peer reviewed
Open access
The MEG experiment, designed to search for the Formula omitted decay, completed data-taking in 2013 reaching a sensitivity level of Formula omitted for the branching ratio. In order to increase the ...sensitivity reach of the experiment by an order of magnitude to the level of Formula omitted, a total upgrade, involving substantial changes to the experiment, has been undertaken, known as MEG II. We present both the motivation for the upgrade and a detailed overview of the design of the experiment and of the expected detector performance.
Dermanyssus gallinae (Mesostigmata: Dermanyssidae, De Geer, 1778) is an ectoparasite of poultry, suspected to play a role as a vector of Salmonella enterica subsp. enterica ser. Gallinarum. Despite ...an association between them being reported, the actual dynamics in field remain unclear. Therefore, the present study aimed to confirm the interactions among mites, pathogen and chickens. The study was carried out in an industrial poultry farm infested by D. gallinae, during an outbreak of fowl typhoid. The presence of S. Gallinarum in mites was assessed and quantified by a semi‐nested polymerase chain reaction (PCR) and real‐time PCR, respectively, in mites collected during two subsequent productive cycles and the sanitary break. The anti‐group D Salmonella antibodies were quantified by an enzyme‐linked immunosorbent assay. During the outbreak and the sanitary break, S. Gallinarum was constantly present in mites. In the second cycle, scattered positivity was observed, although hens did not exhibit signs of fowl typhoid, as a result of the vaccination with BIO‐VAC SGP695 (Fatro, Ozzano Emilia Bo, Italy). The data strongly suggest that D. gallinae acts as reservoir of S. Gallinarum, thus allowing the pathogen to persist in farms. Furthermore, the present study has highlighted the interactions among D. gallinae, S. Gallinarum and hens with respect to enhancing the mite‐mediated circulation of S. Gallinarum in an infested poultry farm.
The association between Dermanyssus gallinae and Samonella enterica subsp. enterica ser. Gallinarum has been confirmed in the field during a fowl typhoid outbreak in an infested industrial poultry farm.
The infection rate of Dermanyssus gallinae and the microbial load in mites were numerically related to the circulation of Salmonella Gallinarum in the flock.
Preventive measures, such as a proper vaccination programme, may prevent the infection and the common reinfection of the new flocks after the sanitary break.
•IBV-QX vaccine and virulent progenitor have comparable RNA titers in the trachea.•IBV-QX vaccine shows delayed induction of lesions in the respiratory tract.•IBV-QX vaccine has reduced ability to ...disseminate from the site of inoculation to other target tissues.•IBV-QX vaccine displays no nephropathogenicity in SPF broilers.•Ascending virus transport seems to contribute to kidney infection.
Infectious bronchitis (IB) is a highly contagious respiratory disease of poultry, caused by the avian coronavirus infectious bronchitis virus (IBV). Currently, one of the most relevant genotypes circulating worldwide is IBV-QX (GI-19), for which vaccines have been developed by passaging virulent QX strains in embryonated chicken eggs. Here we explored the attenuated phenotype of a commercially available QX live vaccine, IB Primo QX, in specific pathogens free broilers. At hatch, birds were inoculated with QX vaccine or its virulent progenitor IBV-D388, and postmortem swabs and tissues were collected each day up to eight days post infection to assess viral replication and morphological changes. In the trachea, viral RNA replication and protein expression were comparable in both groups. Both viruses induced morphologically comparable lesions in the trachea, albeit with a short delay in the vaccinated birds. In contrast, in the kidney, QX vaccine viral RNA was nearly absent, which coincided with the lack of any morphological changes in this organ. This was in contrast to high viral RNA titers and abundant lesions in the kidney after IBV D388 infection. Furthermore, QX vaccine showed reduced ability to reach and replicate in conjunctivae and intestines including cloaca, resulting in significantly lower titers and delayed protein expression, respectively. Nephropathogenic IBVs might reach the kidney also via an ascending route from the cloaca, based on our observation that viral RNA was detected in the cloaca one day before detection in the kidney. In the kidney distal tubular segments, collecting ducts and ureter were positive for viral antigen. Taken together, the attenuated phenotype of QX vaccine seems to rely on slower dissemination and lower replication in target tissues other than the site of inoculation.
A syndrome characterized by apathy, diarrhoea and high mortality of nestlings was observed in a flock of pet birds of the family Estrildidae. Enlargement of the liver, pulmonary congestion and urate ...accretions in the kidney were observed. Microscopically, there was glomerular atrophy, oedema and congestion of the lungs and necrosis and fibrosis of the liver. Cowdry type B intranuclear inclusion bodies were detected in the tissues. Polyomavirus was detected by polymerase chain reaction. The entire genome of the virus was amplified and sequenced, revealing 99 % identity to the sequence of finch polyomavirus isolated from the Eurasian bullfinch (family Fringillidae).
The design of the MEG II experiment Baldini, A. M.; Baracchini, E.; Bemporad, C. ...
The European physical journal. C, Particles and fields,
05/2018, Volume:
78, Issue:
5
Journal Article
Peer reviewed
Open access
The MEG experiment, designed to search for the
μ
+
→
e
+
γ
decay, completed data-taking in 2013 reaching a sensitivity level of
5.3
×
10
-
13
for the branching ratio. In order to increase the ...sensitivity reach of the experiment by an order of magnitude to the level of
6
×
10
-
14
, a total upgrade, involving substantial changes to the experiment, has been undertaken, known as MEG II. We present both the motivation for the upgrade and a detailed overview of the design of the experiment and of the expected detector performance.
Abstract
Background
Patients with type 1 (T1DM) and type 2 (T2DM) diabetes are at increased risk of vaccine-preventable diseases and their complications. The National Vaccinal Prevention Plan ...2017-2019 includes specific recommendations for patients with diabetes, which in 2018 have been included in the standards of care of the Italian Diabetes Society (SID) and Italian Association of Clinical Diabetologists (AMD).
Methods
Adult diabetic patients attending the Diabetes clinic at the San Raffaele Hospital, a large teaching hospital in Milan, Italy, were surveyed with a questionnaire to investigate self-reported vaccinal history, knowledge, attitudes and practice on the recommended vaccinations, vaccinal hesitancy and information sources on the topic.
Results
Between May 16 and November 11, 2019 N = 222 T1DM and N = 57 T2DM patients were enrolled. 25.2% of T1DM and 26.3% of T2DM patients did get vaccinated annually against the flu in the past 3 year. 85.0% of T2DM patients were vaccinated at the General Practitioner's (GP) clinic, while 54.9% of T1DM patients were vaccinated by their GP and 24.4% from territorial vaccinal clinics. 54.5% of T1DM and 50.0% of T2DM patients chose not to be vaccinated because they didn't consider the vaccine useful, while the second most common reason was that they have not been advised by healthcare personnel.
Conclusions
We documented a low coverage and knowledge about influenza vaccination. Patients with diabetes trusted information and advice of diabetologists and GPs about vaccination and claimed that they would be more likely to vaccinate if vaccination were offered in the diabetes clinic. Implementing training programs for professionals and educational campaigns for patients with diabetes may improve vaccinal coverage.
Key messages
Vaccination coverage in T1DM and T2DM patients is low due to vaccination hesitancy. A strong action from care givers, in order to raise vaccination awareness in this at-risk population, is needed.
The ultra-low mass cylindrical drift chamber designed for the MEG II experiment is a challenging apparatus made of 1728 ϕ=20μm gold plated tungsten sense wires, 7680 ϕ=40μm and 2496 ϕ=50μm silver ...plated aluminium field wires. Because of electrostatic stability requirements all the wires have to be stretched at mechanical tensions of ∼25, ∼19 and ∼29g respectively which must be controlled at a level better than 0.5g. This chamber is presently in acquisition, but during its construction ∼100 field wires broke, because of chemical corrosion induced by the atmospheric humidity.
On the basis of the experience gained with this chamber we decided to build a new one, equipped with a different type of wires less sensitive to corrosion. The choice of the new wire required a deep inspection of its characteristics and one of the main tools for doing this is a system for measuring the wire tension by means of the resonant frequency technique, which is described in this paper. The system forces the wires to oscillate by applying a sinusoidal signal at a known frequency, and then measures the variation of the capacitance between a wire and a common ground plane as a function of the external signal frequency. We present the details of the measuring system and the results obtained by scanning the mechanical tensions of two samples of MEG II cylindrical drift chamber wires and discuss the possible improvements of the experimental apparatus and of the measuring technique.
The Helium based ultra-low mass and high granularity Drift Chambers are an optimal solution as tracking systems in modern High Energy Physics experiments for the search of extremely rare processes ...(MEG-II) and for experiments at future high luminosity e+e− colliders (FCC/CEPC). For the search of rare processes in the cLFV sector the tracking resolutions are dominated by multiple scattering contributions and this impose the use of a ultra-light tracking system. For the future collider in addition to a high tracking performances, a great particle identification (PID) capabilities can have a great impact. We present how, in Helium based gas mixtures, by counting and measuring the arrival times of each individual ionization cluster and by using statistical tools it is possible to have a bias free estimate of the impact parameter and a better PID discrimination by using the dN/dx technique instead of the dE/dx.
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