Grafting typically offers a shortcut to breed tree orchards throughout a multidimensional space of traits. Despite an overwhelming spectrum of rootstock-mediated effects on scion traits observed ...across several species, the exact nature and mechanisms underlying the rootstock-mediated effects on scion traits in cacao (
L.) plants often remain overlooked. Therefore, we aimed to explicitly quantify rootstock-mediated genetic contributions in recombinant juvenile cacao plants across target traits, specifically cadmium (Cd) uptake, and its correlation with growth and physiological traits. Content of chloroplast pigments, fluorescence of chlorophyll
, leaf gas exchange, nutrient uptake, and plant biomass were examined across ungrafted saplings and target rootstock × scion combinations in soils with contrasting levels of Cd. This panel considered a total of 320 progenies from open-pollinated half-sib families and reciprocal full-sib progenies (derived from controlled crosses between the reference genotypes IMC67 and PA121). Both family types were used as rootstocks in grafts with two commercial clones (ICS95 and CCN51) commonly grown in Colombia. A pedigree-based best linear unbiased prediction (
-BLUP) mixed model was implemented to quantify rootstock-mediated narrow-sense heritability (
) for target traits. A Cd effect measured on rootstocks before grafting was observed in plant biomass, nutrient uptake, and content of chloroplast pigments. After grafting, damage to the Photosystem II (PSII) was also evident in some rootstock × scion combinations. Differences in the specific combining ability for Cd uptake were mostly detected in ungrafted rootstocks, or 2 months after grafting with the clonal CCN51 scion. Moderate rootstock effects (
> 0.1) were detected before grafting for five growth traits, four nutrient uptake properties, and chlorophylls and carotenoids content (
= 0.19, 95% CI 0.05-0.61,
= 0.7). Such rootstock effects faded (
< 0.1) when rootstock genotypes were examined in soils without Cd, or 4 months after grafting. These results suggest a pervasive genetic conflict between the rootstock and the scion genotypes, involving the triple rootstock × scion × soil interaction when it refers to Cd and nutrient uptake, early growth, and photosynthetic process in juvenile cacao plants. Overall, deepening on these findings will harness early breeding schemes of cacao rootstock genotypes compatible with commercial clonal scions and adapted to soils enriched with toxic levels of Cd.
•A small-scale 1:34 road tunnel setup was modeled by Fire Dynamics Simulator (FDS).•Turbulent transport through DS-TJ air curtains was numerically studied.•Kelvin-Helmholtz and Görtler-type ...structures promote heat leak on the impinging zone.•Jet velocities and temperatures control heat confinement by DS-TJ air curtain tilt.•DS-TJ air curtains reduce heat diffusion, justifying them as a confinement solution.
Simulations with the Fire Dynamics Simulator (FDS) were performed to study the ability of Double Stream–Twin Jets (DS-TJ) air curtains to confine heat. Numerical simulations aimed to model a 1:34 scale tunnel, equipped with a heat source and two DS-TJ air curtains emerging from the tunnel ceiling. The novelty of this work was the imposition of low jet velocities to reduce fans power as much as possible, while ensuring confinement. Streamwise velocity and temperature were contrasted with experimental data.
We observed a systematic deviation towards the faster jet, thus providing a better confinement than perfectly vertical curtains. Kelvin-Helmholtz instabilities related to turbulent transport were identified in the mixing layer, which induced leakage at the impingement on the tunnel ground. These instabilities were observed to amplify Reynolds stresses, playing a role on the flow regimes, the mass and heat balance and the confinement achieved.
Temperature reached plateau values at both sides of the DS-TJ air curtain, thus heat diffusion was attenuated. Temperature drop in the protected zone occurred and the confinement effect was increased. Turbulent heat flux direction depended on the velocity difference between both jets, which defined the confinement characteristics.
•Random non-autonomous logistic-type differential equations are studied.•Random Variable Transformation method and Karhunen–Love expansion are combined.•First probability density function of the ...solution stochastic process is determined.•Numerical simulations for the mean, variance and PDF of the solution are performed.•A wide range of PDFs for input data are considered in numerical experiments.
This paper deals with the study, from a probabilistic point of view, of logistic-type differential equations with uncertainties. We assume that the initial condition is a random variable and the diffusion coefficient is a stochastic process. The main objective is to obtain the first probability density function, f1(p, t), of the solution stochastic process, P(t, ω). To achieve this goal, first the diffusion coefficient is represented via a truncation of order N of the Karhunen–Loève expansion, and second, the Random Variable Transformation technique is applied. In this manner, approximations, say f1N(p,t), of f1(p, t) are constructed. Afterwards, we rigorously prove that f1N(p,t)⟶f1(p,t) as N → ∞ under mild conditions assumed on input data (initial condition and diffusion coefficient). Finally, three illustrative examples are shown.
How does the news media respond to health emergencies abroad? Between 2015 and 2018, Zika virus spread rapidly throughout Latin America before arriving in the continental United States. Despite the ...risks to adults and newborns, it is unclear how media coverage developed and framed the threat for its audience. In this paper, we argue that while the frequency of coverage was responsive to infections, its content failed to promote proactive health behaviour. To assess these claims, we analyse each of 442 articles dealing with Zika virus published by The New York Times from 2015–18. We find that the amount of coverage reflected infections but did not change once the disease emerged in the US. Furthermore, content analysis using Linguistic Inquiry and Word Count software reveals that coverage emphasised differences between communities (those affected and those at home) and that present and past time orientations dominated coverage as opposed to future time orientations.
نبذة مختصرة
كيف تستجيب وسائل الإعلام لحالات الطوارئ الصحية في الخارج ؟ من عام 2015 إلى عام 2018، انتشر مرض فيروس زيكا بسرعة في جميع أنحاء أمريكا اللاتينية قبل وصوله إلى الولايات المتحدة القارية. على الرغم من المخاطر التي يتعرض لها البالغون والأطفال حديثي الولادة، فمن غير الواضح كيف استجابت التغطية الإعلامية وصورت المخاطر لمتتبعيها. في هذا المقال، نناقش بأنه على الرغم من أن تكرار التغطية كان مستجيباً لانتشار العدوى إلا أن محتواها فشل في تعزيز السلوك الصحي الاستباقي. لتقييم هذه الادعاءات، سنقوم بتحليل كل مقال يتناول فيروس زيكا تم نشره بين 2015–2018 بواسطة صحيفة نيويورك تايمز. نجد أن مقدار التغطية ينعكس على مدى انتشار العدوى لكنها لم تتغير بمجرد وصول الفيروس إلى الولايات المتحدة. علاوة على ذلك، يكشف تحليل المحتوى باستخدام برنامج تحليل النصوص LIWC أن التغطية أكدت الاختلافات بين المجتمعات (المتأثرة وتلك الموجودة في المنزل) وأن التوجهات الزمنية الحالية والماضية سيطرت على التغطية بدلاً من التوجهات الزمنية المستقبلية.
الكلمات المفتاحية: الوباء، التأطير، التغطية الإعلامية، الصحة العامة، فيروس زيكا
摘要
新闻媒体如何应对国外的卫生突发事件？从2015年到2018年，寨卡病毒疾病在抵达美国大陆之前迅速蔓延整个拉丁美洲。尽管成人和新生儿面临风险，但目前还不清楚媒体报道是如何为其受众回应和定调风险的。在这篇文章中，我们认为，虽然报道的频率对感染有响应，但其内容未能促进主动的健康行为。为了评估这些主张，我们分析了《纽约时报》在2015–2018年间发表的每一篇涉及寨卡病毒的文章（442篇）。我们发现，报道数量反映了感染情况，但在病毒到达美国后，报道数量并没有改变。此外，使用LIWC文本分析程序进行的内容分析显示，报道强调了群体之间的差异（受影响的人和居家的人），而且现在和过去的时间取向在报道中占主导地位，未来的时间取向并没有占主导。
关键词: 流行病，定调，媒体报道，公共卫生，寨卡病毒
Introduction
Patients with systemic lupus erythematosus (SLE) have cognitive dysfunctions as a neuropsychiatric manifestation, associated with disabling symptoms. However, the presence of other ...medical or psychiatric comorbidities can delay or lead to a misdiagnose.
Objectives
To present a case of a patient with diagnostic difficulty in the face of multiple medical and neurocognitive comorbidities.
Methods
Description of a case report.
Results
19-year-old female, Mexican, unemployed, with incomplete high school, with medical history of preterm birth by cesarean at 30 weeks due to placenta previa, history of early puberty, 4 years evolution of focal epilepsy, 1 year evolution of hypothyroidism and mild depression.
She began her symptoms 4 years ago, characterized by an abrupt onset of memory disturbances, decreased concentration, poor academic performance, infantile behavior, need for affection, alternated with irritability periods, verbal and physical aggression, repetitive and erratic behavior. She went to multiple specialists with different therapeutic approaches without clinical improvement. In 2020, she was referred to our service for evaluation, evidenciating a mild depressive episode and psychotherapeutic treatment was started.
Mental and neurological examination without alterations, normal vital signs, at physical examination: malar rash, oral ulcers, alopecia. Labs: increased erythrocyte sedimentation rate, normocytic anemia, leukopenia, rest normal. An electroencephalogram was requested, without alterations. Simple brain MRI was performed (Figure 1).
Psychological (figure 2) and Neuropsychological tests (table 1) were performed, showing alterations in memory recall and inhibitory control.
Due to the symptoms presented by the patient, SLE was suspected, and rheumatology evaluation was requested, integrating a diagnosis of incomplete SLE, and started treatment. The patient presented symptomatic improvement in cognitive symptoms and systemic signs. Likewise, a genetic evaluation was requested, without meeting the criteria for a genetic syndrome. The patient continues with symptomatic improvement and multidisciplinary treatment.
Total scores
Natural
Normalized
Diagnosis
Orbitomedial
180
83
Mild alteration
Pref-Anterior
22
106
Normal
Dorsolateral
207
88
Normal
BANFE total
409
104
Normal
Image:
Image 2:
Conclusions
Neurocognitive alterations are one of the most frequent manifestations of neurolupus, although its diagnosis and treatment may be delayed in the absence of clinical suspicion, mainly in multi-comorbid patients.
In the case, the patient presented multiple diseases that can explain a picture of neurocognitive impairment, such as epilepsy, depression, hypothyroidism. However, in these cases, a multidisciplinary approach is imperative, requiring to rule out the different causes of the patient’s symptoms.
Disclosure of Interest
None Declared
Mung bean (
Vigna radiata
L.) quality is dependent on seed chemical composition, which in turn determines the benefits of its consumption for human health and nutrition. While mung bean is rich in a ...range of nutritional components, such as protein, carbohydrates and vitamins, it remains less well studied than other legume crops in terms of micronutrients. In addition, mung bean genomics and genetic resources are relatively sparse. The objectives of this research were three-fold, namely: to develop a genome-wide marker system for mung bean based on genotyping by sequencing (GBS), to evaluate diversity of mung beans available to breeders in the United States and finally, to perform a genome-wide association study (GWAS) for nutrient concentrations based on a seven mineral analysis using inductively coupled plasma (ICP) spectroscopy. All parts of our research were performed with 95 cultivated mung bean genotypes chosen from the USDA core collection representing accessions from 13 countries. Overall, we identified a total of 6,486 high quality single nucleotide polymorphisms (SNPs) from the GBS dataset and found 43 marker × trait associations (MTAs) with calcium, iron, potassium, manganese, phosphorous, sulfur or zinc concentrations in mung bean grain produced in either of two consecutive years’ field experiments. The MTAs were scattered across 35 genomic regions explaining on average 22% of the variation for each seed nutrient in each year. Most of the gene regions provided valuable candidate loci to use in future breeding of new varieties of mung bean and further the understanding of genetic control of nutritional properties in the crop. Other SNPs identified in this study will serve as important resources to enable marker-assisted selection (MAS) for nutritional improvement in mung bean and to analyze cultivars of mung bean.
Objective To investigate the effect of an additional review based on reporting guidelines such as STROBE and CONSORT on quality of manuscripts.Design Masked randomised trial.Population Original ...research manuscripts submitted to the Medicina Clínica journal from May 2008 to April 2009 and considered suitable for publication.Intervention Control group: conventional peer reviews alone. Intervention group: conventional review plus an additional review looking for missing items from reporting guidelines.Outcomes Manuscript quality, assessed with a 5 point Likert scale (primary: overall quality; secondary: average quality of specific items in paper). Main analysis compared groups as allocated, after adjustment for baseline factors (analysis of covariance); sensitivity analysis compared groups as reviewed. Adherence to reviewer suggestions assessed with Likert scale.Results Of 126 consecutive papers receiving conventional review, 34 were not suitable for publication. The remaining 92 papers were allocated to receive conventional reviews alone (n=41) or additional reviews (n=51). Four papers assigned to the conventional review group deviated from protocol; they received an additional review based on reporting guidelines. We saw an improvement in manuscript quality in favour of the additional review group (comparison as allocated, 0.25, 95% confidence interval –0.05 to 0.54; as reviewed, 0.33, 0.03 to 0.63). More papers with additional reviews than with conventional reviews alone improved from baseline (22 (43%) v eight (20%), difference 23.6% (3.2% to 44.0%), number needed to treat 4.2 (from 2.3 to 31.2), relative risk 2.21 (1.10 to 4.44)). Authors in the additional review group adhered more to suggestions from conventional reviews than to those from additional reviews (average increase 0.43 Likert points (0.19 to 0.67)).Conclusions Additional reviews based on reporting guidelines improve manuscript quality, although the observed effect was smaller than hypothesised and not definitively demonstrated. Authors adhere more to suggestions from conventional reviews than to those from additional reviews, showing difficulties in adhering to high methodological standards at the latest research phases. To boost paper quality and impact, authors should be aware of future requirements of reporting guidelines at the very beginning of their study. Trial registration and protocol Although registries do not include trials of peer review, the protocol design was submitted to sponsored research projects (Instituto de Salud Carlos III, PI081903).
In this paper we study random non-autonomous second order linear differential equations by taking advantage of the powerful theory of random difference equations. The coefficients are assumed to be ...stochastic processes, and the initial conditions are random variables both defined in a common underlying complete probability space. Under appropriate assumptions established on the data stochastic processes and on the random initial conditions, and using key results on difference equations, we prove the existence of an analytic stochastic process solution in the random mean square sense. Truncating the random series that defines the solution process, we are able to approximate the main statistical properties of the solution, such as the expectation and the variance. We also obtain error
a priori
bounds to construct reliable approximations of both statistical moments. We include a set of numerical examples to illustrate the main theoretical results established throughout the paper. We finish with an example where our findings are combined with Monte Carlo simulations to model uncertainty using real data.
Exploring the genomic architecture of species and populations divergence aids understanding how lineages evolve and adapt, and ultimately can show the repeatability of evolutionary processes. Yet, ...the genomic signatures associated with divergence are still relatively unexplored, leading to a knowledge gap on whether species divergence ultimately differs in its genetic architecture from divergence at other spatial scales (i.e., populations, ecotypes). Our goal in this research was to determine whether genomic islands of speciation are more prone to harbor within-species differentiation due to genomic features, suppressed recombination, smaller effective population size or increased drift, across repeated hierarchically nested levels of divergence. We used two species of
beans with strong genepool and population sub-structure produced by multiple independent domestications each especially in Andean and Mesoamerican / Middle American geographies. We genotyped 22,531 GBS-derived SNP markers in 209 individuals of wild and cultivated
and
. We identified six regions for species-associated divergence. Out of these divergence peaks, 21% were recovered in the four within-species between-genepool comparisons and in the five within-genepool wild-cultivated comparisons (some of the latter did retrieve genuine signatures of the well described multiple domestication syndromes). However, genomic regions with overall high relative differentiation (measured by F
) coincided with regions of low SNP density and regions of elevated delta divergence between-genepools (Δ
), independent of the scale of divergence. The divergence in chromosome Pv10 further coincided with a between-species pericentric inversion. These convergences suggest that shared variants are being recurrently fixed at replicated regions of the genome, and in a similar manner across different hierarchically nested levels of divergence, likely as result of genomic features that make certain regions more prone to accumulate islands of speciation and within-species divergence. In summary, neighboring signatures of speciation, adaptation and domestication in
beans are influenced by ubiquitous genomic constrains, which may continue to fortuitously shape genomic differentiation at various others scales of divergence.
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