Objective
To compare the thermographic pattern of regions of interest (ROI) of respiratory muscles in young asthmatics with and without bronchospasm induced by eucapnic voluntary hyperpnea (EVH).
...Materials and Methods
Cross‐sectional study carried out with 55 young (55% male and 45% females) aged 12.5 ± 3.3 years, divided in nine nonasthmatics, 22 asthmatics without exercise‐induced bronchospasm compatible response (EIB‐cr) and 24 asthmatics with EIB‐cr. The diagnosis of EIB was given to subjects with a fall in forced expiratory volume in the first second (FEV1) ≥ 10% compared to baseline. Thermographic recordings of respiratory muscles were delimited in ROI of the sternocleidomastoid (SCM), pectoral, and rectus abdominis intention area. Thermal captures and FEV1 were taken before and 5, 10, 15 and 30 min after EVH.
Results
Twenty‐four (52.1%) of asthmatics had EIB‐cr. There was a decrease in temperature at 10 min after EVH test in the SCM, pectoral and rectus abdominis ROIs in all groups (both with p < 0.05). There was a decrease in temperature (% basal) in asthmatic with EIB‐cr compared to nonasthmatics in the rectus abdominis area (p < 0.05).
Conclusion
There was a decrease in temperature in the ROIs of different muscle groups, especially in asthmatics. The greater drop in FEV1 observed in individuals with EIB‐cr was initially associated with a decrease in skin temperature, with a difference between the nonasthmatics in the abdominal muscle area. It is likely that this decrease in temperature occurred due to a temporary displacement of blood flow to the most used muscle groups, with a decrease in the region of the skin evaluated in the thermography.
Abstract The aim of this study was to evaluate the effects of chrysin on the ventral prostate of spontaneously hypertensive rats (SHR). Ten‐week‐old male Wistar and SHR rats received 100 mg/kg/day of ...chrysin (TW and TSHR) or 200 µL/day of the dilution vehicle (CW and CSHR) for 70 days. After the treatment, the animals were euthanized and the prostates were dissected out, fixed, and processed for further morphological, immunohistochemical, and biochemical analyses. Blood was collected for serological analysis. Chrysin did not interfere with the blood pressure. Morphologically, the epithelial height increased in TW and decreased in TSHR. Stereology showed an increase in the epithelial and stromal relative frequency, and a decrease in the lumen of TW, whereas the epithelium in TSHR was reduced. Normal alveoli decreased, and hyperplastic alveoli had an increment in TW, whereas in TSHR normal alveoli increased and intense hyperplasia decreased. The secretion area was reduced in TW. Immunohistochemical analysis showed a smaller number of PCNA‐positive cells in TW. Finally, the biochemical analysis showed a reduction in malondialdehyde, carbonylated proteins, superoxide dismutase, and catalase in TW and TSHR. We concluded that the chrysin effect is dependent on the context in which this flavonoid is employed. In normal conditions, the anabolic potential of the chrysin was favored, disrupting the morphology of the prostate. However, when used in animals predisposed to develop hyperplasia, this flavonoid attenuates the hyperplastic status, improving the morphology of the gland.
Synthetically
derived samples of (+)-(6a
S
,11a
S
)-2,3,9-trimethoxypterocarpan (+)-
1
and
its enantiomer (−)-
1
, both of which are examples
of naturally occurring isoflavonoids, were evaluated, ...together with
the corresponding racemate, as cytotoxic agents against the HL-60,
HCT-116, OVCAR-8, and SF-295 tumor cell lines. As a result it was
established that compound (+)-
1
was particularly active
with OVCAR-8 cells being the most sensitive and responding in a dose-dependent
manner. A study of cell viability and drug-induced morphological changes
revealed the compound causes cell death through a mechanism characteristic
of apoptosis. Finally, a computational study of the interactions of
compound (+)-
1
and (
S
)-monastrol, an
established, synthetically derived, potent, and cell-permeant inhibitor
of mitosis, with the kinesin-type protein Eg5 revealed that both bind
to this receptor in a similar manner. Significantly, compound (+)-
1
binds with greater affinity, an effect attributed to the
presence of the associated methoxy groups.
Slower intravenous fluid infusion rates could reduce the formation of tissue edema and organ dysfunction in critically ill patients; however, there are no data to support different infusion rates ...during fluid challenges for important outcomes such as mortality.
To determine the effect of a slower infusion rate vs control infusion rate on 90-day survival in patients in the intensive care unit (ICU).
Unblinded randomized factorial clinical trial in 75 ICUs in Brazil, involving 11 052 patients requiring at least 1 fluid challenge and with 1 risk factor for worse outcomes were randomized from May 29, 2017, to March 2, 2020. Follow-up was concluded on October 29, 2020. Patients were randomized to 2 different infusion rates (reported in this article) and 2 different fluid types (balanced fluids or saline, reported separately).
Patients were randomized to receive fluid challenges at 2 different infusion rates; 5538 to the slower rate (333 mL/h) and 5514 to the control group (999 mL/h). Patients were also randomized to receive balanced solution or 0.9% saline using a factorial design.
The primary end point was 90-day survival.
Of all randomized patients, 10 520 (95.2%) were analyzed (mean age, 61.1 years SD, 17.0 years; 44.2% were women) after excluding duplicates and consent withdrawals. Patients assigned to the slower rate received a mean of 1162 mL on the first day vs 1252 mL for the control group. By day 90, 1406 of 5276 patients (26.6%) in the slower rate group had died vs 1414 of 5244 (27.0%) in the control group (adjusted hazard ratio, 1.03; 95% CI, 0.96-1.11; P = .46). There was no significant interaction between fluid type and infusion rate (P = .98).
Among patients in the intensive care unit requiring fluid challenges, infusing at a slower rate compared with a faster rate did not reduce 90-day mortality. These findings do not support the use of a slower infusion rate.
ClinicalTrials.gov Identifier: NCT02875873.
Treatment survival with biological therapy may be influenced by many factors, and it seems to be different among various rheumatic diseases and biological agents. The goal of the study was to compare ...the drug survival and the causes of discontinuation of anti-tumoral necrosis factor (anti-TNF) therapy in ankylosing spondylitis (AS) with rheumatoid arthritis (RA). Study participants were a cohort from the Brazilian Registry of Biological Therapies in Rheumatic Diseases (BIOBADABRASIL) between 2008 and 2012. The observation time was up to 4 years following the introduction of the first treatment. Gender, age, disease duration, disease activity, comorbidities, and concomitant therapies were assessed. A total of 1303 patients were included: 372 had AS and 931 had RA in which 38.7 % (
n
= 504) used infliximab (IFX), 34.9 % (
n
= 455) used adalimumab (ADA), and 26.4 % (
n
= 344) used etanercept (ETA). The anti-TNF drug survival of patients with AS was 63.08 months (confidence interval (CI) 60.24, 65.92) and patients with RA was 47.5 months (CI 45.65, 49.36). It was significant higher in AS (log-rank;
p
≤ 0.001). Patients with RA discontinued anti-TNF more than patients with AS when adjusted to gender and corticosteroid. The adjHR (95 % CI) was 1.6 (1.14, 2.31). Female patients who were also corticosteroid users, but not of advanced age, have shown lower survival for both diseases (log-rank,
p
≤ 0.001). The discontinuation rate of IFX, but not of ADA or ETA, was significantly higher in RA than in SA; HR (95 % CI) was 2.49 (1.46, 4.24). The main causes of discontinuation were ineffectiveness and adverse event in both diseases. AS patients have better drug survival adjusted to gender, age, and corticosteroid. This results appear to be related to the disease mechanism.
To investigate how quickly active video games, structured and unstructured, provide changes in hemodynamic variables in young adults during a 6-week intervention.
Twenty participants after baseline ...assessments, participants were randomized: structured active videogame (n=6), unstructured active videogame (n=7) and a control group (n=7). Participants played their respective active videogame 3 times a week for 6-weeks (30min-session).
Structured active videogame in exactly 6 weeks shown improvements reducing the heart rate (heart rate; 14% of variation; p<0.05). Otherwise, not confirmed to both active videogame interventions in systolic blood pressure but maintain the diastolic blood pressure during these 6 weeks (systolic blood pressure-unstructured: −2% and Structured: 11%; diastolic blood pressure-unstructured: 0% and structured: 0%; p>0.05).
The 6-week training program with active videogame reduced the heart rate (structured – 6th week). However, active videogames generally do not promoted benefits for normotensive young adults.
Investigar o quão rápido os videogames ativos, estruturados e não estruturados, proporcionam mudanças em variáveis hemodinâmicas em adultos jovens durante uma intervenção de seis semanas.
Foram randomizados 20 participantes após avaliações iniciais: videogame ativo estruturado (n = 6), videogame ativo não estruturado (n = 7) e um grupo controle (n = 7). Os participantes fizeram seu respectivo videogame ativo três vezes por semana durante seis semanas (30 minutos por sessão).
videogame ativo estruturado em exatamente seis semanas mostrou redução da frequência cardíaca (frequência cardíaca; 14% de variação; p < 0,05). Por outro lado, não confirmou em ambas as intervenções de videogame ativo pressão arterial sistólica, porém manteve a pressão arterial diastólica durante essas seis semanas (pressão arterial sistólica não estruturada: -2% e estruturadas: 11%; pressão arterial diastólica não estruturada: 0% e estruturada: 0%; p < 0,05).
O programa de treinamento de seis semanas com videogame ativo reduziu o frequência cardíaca (estruturado seis semanas). Porém, de forma geral, os videogames ativos não promoveram benefícios em adultos jovens normotensos.
Analizar la rapidez con la cual los videojuegos activos, estructurados y no estructurados, generan cambios en las variables hemodinámicas en adultos jóvenes durante una intervención de 6 semanas.
Se distribuyó aleatoriamente a 20 participantes según las evaluaciones iniciales: videojuego activo estructurado (n = 6), videojuego activo no estructurado (n = 7) y grupo control (n = 7). Los participantes jugaron con sus respectivos videojuegos activos 3 veces por semana durante 6 semanas (sesiones de 30 minutos).
Los videojuegos activos estructurados en exactamente 6 semanas mostraron que reducían la frecuencia cardíaca (frecuencia cardiaca; 14% de la variación; p <0,05). En cambio, no se confirmó en ambas intervenciones de videojuegos activos la presión arterial sistólica, pero se mantuvo la presión arterial diastólica durante estas 6 semanas (presión arterial sistólica en no estructurados: -2% y en estructurados: 11%; presión arterial diastólica en no estructurados: 0% y en estructurados: 0%, p >0,05).
El programa de entrenamiento de 6 semanas con videojuegos activos redujo la frecuencia cardiaca (estructurado - sexta semana). Sin embargo, de forma general los videojuegos activos no promueven beneficios en adultos jóvenes normotensos.
DFT calculations using the B3LYP and PBE1PBE functionals with the standard 6-31G(d) and 6-311+G(2d,p) basis sets were carried out for the 3-(2-phenylhydrazone)-naphthalene-1,2,4-trione system in ...solution (dmso) and in the gas phase, and showed the keto-hydrazone forms (rotamers Ia and Ib) to be more stable than the enol-azo forms (rotamers IIa and IIb, by about 14 kcal mol-1) and III (by approximately 6 kcal mol-1), independently of the nature of the substituent in the phenylene ring. These results were confirmed by spectroscopic data on the derivatives HL1-HL13, obtained from 2-hydroxy-1,4-naphthoquinone and arylamines (R = 4-OMe, 4-N2-C6H5, 4-Cl, 4-I, 3-I, 2-I, 4-COOH, 3-COOH, 4-CN, 3-CN, 4-NO2, 3-NO2, 2-NO2). The in vitro antitumor (against SF-295, HCT-8, MDAMB-435 and HL-60 cancer cell lines) and antibacterial activities (Bacillus cereus, Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa) of compounds HL1-HL13 and of their respective copper(II) complexes, Cu(L1-13)2, were tested. In general, these compounds exhibited low antibacterial activity, except for HL5 (R = 3-I), more active than the control; however, the corresponding complex was inactive. In contrast, increased cytotoxicity was observed upon complexation. Complex Cu(L13)2 (R = 3-NO2) presented moderate cytotoxicity against human leukemia (HL-60).
Aedes aegypti (L.), the main vector of dengue fever in Brazil, has been controlled with the use of massive chemical products, contributing to the development of resistance and decreasing the insect ...control efficiency. The control of dipterans with bioinsecticides based on Bacillus thuringiensis has been satisfactory, due to the production of insecticidal proteins denominated Cry (crystal), Cyt (cytolytic) toxins and Chi (chitinase), and to the synergistic effects among them. The present work aimed to select B. thuringiensis isolates efficient against A. aegypti larvae. A bacterial collection containing 1,073 isolates of B. thuringiensis, obtained from different locations of Brazilian territory, had the DNA isolated and submitted to PCR amplifications using specific primers for cry4Aa, cry4Ba, cry11Aa, cry11Ba, cyt1Aa, cyt1Ab, cyt2Aa and chi genes. For the LC50 and LC90 determination, the entomopathogenic isolates were evaluated by selective and quantitative bioassays. Only 45 isolates (4.2%) presented amplicons for the cry and cyt genes. The chi gene sequence was detected in 25 (54.3%) of those isolates. From the 45 isolates submitted to the selective bioassays, 13 caused 100% mortality of A. aegypti larvae. The identification of cry, cyt and chi genes of B. thuringiensis and the toxicity analysis on A. aegypti led to the selection of a set of isolates that have the potential to be used in the formulation of new bioinsecticides.
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