Der Gebäudebestand benötigt rund 30 % des gesamten Endenergiebedarfes in Deutschland. Der Erfolg der Wärmewende hängt daher maßgeblich vom Einsparpotenzial im Gebäudebestand ab. Dazu gehört neben der ...Sanierung der Gebäudehülle auch der Einsatz einer klimaneutralen Wärmebereitstellung. Für dieses Ziel ist es vorteilhaft und notwendig, die Anlagentechnik von fossilen auf erneuerbare Energieträger umzurüsten. Derzeit gilt zu Recht die Wärmepumpe als Schlüsseltechnologie für das Gelingen der Wärmewende. Dieser Beitrag untersucht den Autarkiegrad der Kombination Wärmepumpe mit Photovoltaikanlage genauer. Um den Autarkiegrad bestimmen zu können, wird für ein konkretes Praxisbeispiel eine Simulationsrechnung mit dem Programm PV*SOL erstellt. Als maßgebend für die Auswertung wird der lastgerechte Autarkiegrad in Abhängigkeit der damit verbundenen Investitionskosten bestimmt und dargestellt.Herrn Prof. Dr.-Ing. Nabil A. Fouad zur Vollendung des 60. Lebensjahres gewidmet
Der Gebäudebestand benötigt rund 30 % des gesamten Endenergiebedarfes in Deutschland. Der Erfolg der Wärmewende hängt daher maßgeblich vom Einsparpotenzial im Gebäudebestand ab. Dazu gehört neben der ...Sanierung der Gebäudehülle auch der Einsatz einer klimaneutralen Wärmebereitstellung. Für dieses Ziel ist es vorteilhaft und notwendig, die Anlagentechnik von fossilen auf erneuerbare Energieträger umzurüsten. Derzeit gilt zu Recht die Wärmepumpe als Schlüsseltechnologie für das Gelingen der Wärmewende. Dieser Beitrag untersucht den Autarkiegrad der Kombination Wärmepumpe mit Photovoltaikanlage genauer. Um den Autarkiegrad bestimmen zu können, wird für ein konkretes Praxisbeispiel eine Simulationsrechnung mit dem Programm PV*SOL erstellt. Als maßgebend für die Auswertung wird der lastgerechte Autarkiegrad in Abhängigkeit der damit verbundenen Investitionskosten bestimmt und dargestellt.
Translation
Effects of replacing a gas heating system with an outdoor air heat pump in an EFH in terms of CO2 savings, self‐sufficiency and cost‐effectiveness
In Germany, existing buildings account for around 30 % of the total final energy demand. The success of the heating transition therefore depends on the saving potential in existing buildings. In addition to the refurbishment of the building envelope, this also includes the use of climate‐neutral heat supply. To achieve this goal, it is advantageous and necessary to convert the system technology from fossil fuels to renewable energy sources. At present, the heat pump is rightly regarded as the key technology for the success of the heating transition. This article analyses the degree of self‐sufficiency of the combination of heat pump and photovoltaic system in detail. In order to determine the degree of self‐sufficiency, an existing single‐family house as practical example has been analyzed using a building simulation by PV*SOL programme. The load‐based degree of self‐sufficiency is determined and presented as a function of the associated investment costs.
This year, organic farmers, advisors and researchers in the West of Germany celebrate the 25th anniversary of the foundation of an organic research and demonstration network. Established to support ...and improve organic farming systems, the network is funded by the federal state of North Rhine-Westphalia and is organized around 30 participating pilot farms called Leitbetriebe (‘leading farms’), with support from the university of Bonn and the Chamber of Agriculture For each of the practice-oriented research topics covered in this network, the typical five-year cycle of research involves a first year of experimental exploration and literature studies. The farmers participating in the pilot farm project discuss and select the topics of priority and review the research plans proposed by the scientists. This is followed by three years of systematic replicated field trials on several farms, and a final year of demonstration and evaluation, where the presentation of results is jointly done by farmers and researchers.
We review the rich literature on behavioural responses of aphids (Hemiptera: Aphididae) to stimuli of different colours. Only in one species there are adequate physiological data on spectral ...sensitivity to explain behaviour crisply in mechanistic terms. Because of the great interest in aphid responses to coloured targets from an evolutionary, ecological and applied perspective, there is a substantial need to expand these studies to more species of aphids, and to quantify spectral properties of stimuli rigorously. We show that aphid responses to colours, at least for some species, are likely based on a specific colour opponency mechanism, with positive input from the green domain of the spectrum and negative input from the blue and/or UV region. We further demonstrate that the usual yellow preference of aphids encountered in field experiments is not a true colour preference but involves additional brightness effects. We discuss the implications for agriculture and sensory ecology, with special respect to the recent debate on autumn leaf colouration. We illustrate that recent evolutionary theories concerning aphid–tree interactions imply far-reaching assumptions on aphid responses to colours that are not likely to hold. Finally we also discuss the implications for developing and optimising strategies of aphid control and monitoring.
Molecular subtypes predict prognosis in muscle-invasive bladder cancer (MIBC) and are explored as predictive markers. To provide a common base for molecular subtyping and facilitate clinical ...applications, a consensus classification has been developed. However, methods to determine consensus molecular subtypes require validation, particularly when FFPE specimens are used. Here, we aimed to evaluate two gene expression analysis methods on FFPE samples and to compare reduced gene sets to classify tumors into molecular subtypes.
RNA was isolated from FFPE blocks of 15 MIBC patients. Massive analysis of 3' cDNA ends (MACE) and the HTG transcriptome panel (HTP) were used to retrieve gene expression. We used normalized, log2-transformed data to call consensus and TCGA subtypes with the consensusMIBC package for R using all available genes, a 68-gene panel (ESSEN1), and a 48-gene panel (ESSEN2).
Fifteen MACE-samples and 14 HTP-samples were available for molecular subtyping. The 14 samples were classified as Ba/Sq in 7 (50%), LumP in 2 (14.3%), LumU in 1 (7.1%), LumNS in 1 (7.1%), stroma-rich in 2 (14.3%) and NE-like in 1 (7.1%) case based on MACE- or HTP-derived transcriptome data. Consensus subtypes were concordant in 71% (10/14) of cases when comparing MACE with HTP data. Four cases with aberrant subtypes had a stroma-rich molecular subtype with either method. The overlap of the molecular consensus subtypes with the reduced ESSEN1 and ESSEN2 panels were 86% and 100%, respectively, with HTP data and 86% with MACE data.
Determination of consensus molecular subtypes of MIBC from FFPE samples is feasible using various RNA sequencing methods. Inconsistent classification mainly involves the stroma-rich molecular subtype, which may be the consequence of sample heterogeneity with (stroma)-cell sampling bias and highlights the limitations of bulk RNA-based subclassification. Classification is still reliable when analysis is reduced to selected genes.
Abstract
Novel therapeutic strategies aiming at improving the healing process after an acute myocardial infarction are currently under intense investigation. The mouse model plays a central role for ...deciphering the underlying mechanisms on a molecular and cellular level. Therefore, we intended to assess in-vivo post-infarct remodeling as comprehensively as possible using an expedient native magnetic resonance imaging (MRI) in the two most prominent infarct models, permanent ligation (PL) of the left anterior descending artery (LAD) versus ischemia reperfusion (I/R). Mice were subjected to either permanent or transient (45 min) occlusion of the LAD. After 3 weeks, examinations were performed with a 7-Tesla small animal MRI system. Data analysis was performed with the freely available software Segment. PL resulted in a massive dilation of the left ventricle, accompanied by hypertrophy of the non-infarcted myocardium and a decline of contractile function. These effects were less pronounced following I/R compared to healthy animals. Single plane assessments were not sufficient to capture the specific differences of left ventricular (LV) properties between the two infarct models. Bulls-eye plots were found to be an ideal tool for qualitative LV wall assessment, whereas a multi-slice sector-based analysis of wall regions is ideal to determine differences in hypertrophy, lateral wall thinning and wall thickening on a quantitative level. We combine the use of polar map-based analysis of LV wall properties with volumetric measurements using simple CINE CMR imaging. Our strategy represents a versatile and easily available tool for serial assessment of the LV during the remodeling process. Our study contributes to a better understanding of the effects of novel therapies targeting the healing of damaged myocardium.
Cystic fibrosis is the most common autosomal recessive disorder in white people, with a frequency of about 1 in 2500 livebirths. Discovery of the mutated gene encoding a defective chloride channel in ...epithelial cells—named cystic fibrosis transmembrane conductance regulator (CFTR)—has improved our understanding of the disorder's pathophysiology and has aided diagnosis, but has shown the disease's complexity. Gene replacement therapy is still far from being used in patients with cystic fibrosis, mostly because of difficulties of targeting the appropriate cells. Life expectancy of patients with the disorder has been greatly increased over past decades because of better notions of symptomatic treatment strategies. Here, we summarise advances in understanding and treatment of cystic fibrosis, focusing on pulmonary disease, which accounts for most morbidity and deaths.
Glioblastomas are incurable tumors infiltrating the brain. A subpopulation of glioblastoma cells forms a functional and therapy-resistant tumor cell network interconnected by tumor microtubes (TMs). ...Other subpopulations appear unconnected, and their biological role remains unclear. Here, we demonstrate that whole-brain colonization is fueled by glioblastoma cells that lack connections with other tumor cells and astrocytes yet receive synaptic input from neurons. This subpopulation corresponds to neuronal and neural-progenitor-like tumor cell states, as defined by single-cell transcriptomics, both in mouse models and in the human disease. Tumor cell invasion resembled neuronal migration mechanisms and adopted a Lévy-like movement pattern of probing the environment. Neuronal activity induced complex calcium signals in glioblastoma cells followed by the de novo formation of TMs and increased invasion speed. Collectively, superimposing molecular and functional single-cell data revealed that neuronal mechanisms govern glioblastoma cell invasion on multiple levels. This explains how glioblastoma’s dissemination and cellular heterogeneity are closely interlinked.
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•Molecular glioblastoma cell states are related to cell functions•Neuronal-like, single-glioblastoma cells drive brain invasion•Glioblastoma invasion resembles multiple traits of neuronal development•Neurogliomal synapses stimulate neurite-like tumor microtubes crucial for invasion
Glioblastoma cells with neuronal features drive brain invasion.
Background Underweight and severe and morbid obesity are associated with highly elevated risks of adverse health outcomes. We estimated trends in mean body-mass index (BMI), which characterises its ...population distribution, and in the prevalences of a complete set of BMI categories for adults in all countries. Methods We analysed, with use of a consistent protocol, population-based studies that had measured height and weight in adults aged 18 years and older. We applied a Bayesian hierarchical model to these data to estimate trends from 1975 to 2014 in mean BMI and in the prevalences of BMI categories (<18.5 kg/m2 underweight, 18.5 kg/m2 to <20 kg/m2, 20 kg/m2 to <25 kg/m2, 25 kg/m2 to <30 kg/m2, 30 kg/m2 to <35 kg/m2, 35 kg/m2 to <40 kg/m2, greater than or equal to 40 kg/m2 morbidobesity), by sex in 200 countries and territories, organised in 21 regions. We calculated the posterior probability of meeting the target of halting by 2025 the rise in obesity at its 2010 levels, if post-2000 trends continue. Findings We used 1698 population-based data sources, with more than 19.2 million adult participants (9.9 million men and 9.3 million women) in 186 of 200 countries for which estimates were made. Global age-standardised mean BMI increased from 21.7 kg/m2 (95% credible interval 21.3-22.1) in 1975 to 24.2 kg/m2 (24.0-24.4) in 2014 in men, and from 22.1 kg/m2 (21.7-22.5) in 1975 to 24.4 kg/m2 (24.2-24.6) in 2014 in women. Regional mean BMIs in 2014 for men ranged from 21.4 kg/m2 in central Africa and south Asia to 29.2 kg/m2 (28.6-29.8) in Polynesia and Micronesia; for women the range was from 21.8 kg/m2 (21.4-22.3) in south Asia to 32.2 kg/m2 (31.5-32.8) in Polynesia and Micronesia. Over these four decades, age-standardised global prevalence of underweight decreased from 13.8% (10.5-17.4) to 8.8% (7.4-10.3) in men and from 14.6% (11.6-17.9) to 9.7% (8.3-11.1) in women. South Asia had the highest prevalence of underweight in 2014, 23.4% (17.8-29.2) in men and 24.0% (18.9-29.3) in women. Age-standardised prevalence of obesity increased from 3.2% (2.4-4.1) in 1975 to 10.8% (9.7-12.0) in 2014 in men, and from 6.4% (5.1-7.8) to 14.9% (13.6-16.1) in women. 2.3% (2.0-2.7) of the world's men and 5.0% (4.4-5.6) of women were severely obese (ie, have BMI greater than or equal to 35 kg/m2). Globally, prevalence of morbid obesity was 0.64% (0.46-0.86) in men and 1.6% (1.3-1.9) in women. Interpretation If post-2000 trends continue, the probability of meeting the global obesity target is virtually zero. Rather, if these trends continue, by 2025, global obesity prevalence will reach 18% in men and surpass 21% in women; severe obesity will surpass 6% in men and 9% in women. Nonetheless, underweight remains prevalent in the world's poorest regions, especially in south Asia. Funding Wellcome Trust, Grand Challenges Canada.
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