Objective
An association between bipolar disorder and cognitive impairment has repeatedly been described, even for euthymic patients. Findings are inconsistent both across primary studies and ...previous meta‐analyses. This study reanalysed 31 primary data sets as a single large sample (N = 2876) to provide a more definitive view.
Method
Individual patient and control data were obtained from original authors for 11 measures from four common neuropsychological tests: California or Rey Verbal Learning Task (VLT), Trail Making Test (TMT), Digit Span and/or Wisconsin Card Sorting Task.
Results
Impairments were found for all 11 test‐measures in the bipolar group after controlling for age, IQ and gender (Ps ≤ 0.001, E.S. = 0.26–0.63). Residual mood symptoms confound this result but cannot account for the effect sizes found. Impairments also seem unrelated to drug treatment. Some test‐measures were weakly correlated with illness severity measures suggesting that some impairments may track illness progression.
Conclusion
This reanalysis supports VLT, Digit Span and TMT as robust measures of cognitive impairments in bipolar disorder patients. The heterogeneity of some test results explains previous differences in meta‐analyses. Better controlling for confounds suggests deficits may be smaller than previously reported but should be tracked longitudinally across illness progression and treatment.
We present a catalog of mean proper motions and membership probabilities of individual stars for optically visible open clusters, which have been determined using data from the UCAC4 catalog in a ...homogeneous way. The mean proper motion of the cluster and the membership probabilities of the stars in the region of each cluster were determined by applying the statistical method in a modified fashion. In this study, we applied a global optimization procedure to fit the observed distribution of proper motions with two overlapping normal bivariate frequency functions, which also take the individual proper motion errors into account. For 724 clusters, this is the first determination of proper motion, and for the whole sample, we present results with a much larger number of identified astrometric member stars. Furthermore, it was possible to estimate the mean radial velocity of 364 clusters (102 unpublished so far) with the stellar membership using published radial velocity catalogs. These results provide an increase of 30% and 19% in the sample of open clusters with a determined mean absolute proper motion and mean radial velocity, respectively.
Bacteriophages are the most abundant and diverse entities in the biosphere, and this diversity is driven by constant predator-prey evolutionary dynamics and horizontal gene transfer. Phage genome ...sequences are under-sampled and therefore present an untapped and uncharacterized source of genetic diversity, typically characterized by highly mosaic genomes and no universal genes. To better understand the diversity and relationships among phages infecting human pathogens, we have analysed the complete genome sequences of 205 phages of Staphylococcus sp.
These are predicted to encode 20,579 proteins, which can be sorted into 2139 phamilies (phams) of related sequences; 745 of these are orphams and possess only a single gene. Based on shared gene content, these phages were grouped into four clusters (A, B, C and D), 27 subclusters (A1-A2, B1-B17, C1-C6 and D1-D2) and one singleton. However, the genomes have mosaic architectures and individual genes with common ancestors are positioned in distinct genomic contexts in different clusters. The staphylococcal Cluster B siphoviridae are predicted to be temperate, and the integration cassettes are often closely-linked to genes implicated in bacterial virulence determinants. There are four unusual endolysin organization strategies found in Staphylococcus phage genomes, with endolysins predicted to be encoded as single genes, two genes spliced, two genes adjacent and as a single gene with inter-lytic-domain secondary translational start site. Comparison of the endolysins reveals multi-domain modularity, with conservation of the SH3 cell wall binding domain.
This study provides a high-resolution view of staphylococcal viral genetic diversity, and insights into their gene flux patterns within and across different phage groups (cluster and subclusters) providing insights into their evolution.
ABSTRACT
In this study, we follow up our recent paper (Monteiro et al. 2020) and present a homogeneous sample of fundamental parameters of open clusters in our Galaxy, entirely based on Gaia DR2 ...data. We used published membership probability of the stars derived from Gaia DR2 data and applied our isochrone fitting code, updated as in Monteiro et al. (2020), to GBP and GRPGaia DR2 data for member stars. In doing this, we take into account the nominal errors in the data and derive distance, age, and extinction of each cluster. This work therefore provides parameters for 1743 open clusters and, as a by-product, a list of likely not physical or dubious open clusters is provided as well for future investigations. Furthermore, it was possible to estimate the mean radial velocity of 831 clusters (198 of which are new and unpublished so far), using stellar radial velocities from Gaia DR2 catalogue. By comparing the open cluster distances obtained from isochrone fitting with those obtained from a maximum likelihood estimate of individual member parallaxes, we found a systematic offset of (−0.05 ± 0.04) mas.
Aims
To investigate the probiotic potential of yeasts isolated from naturally fermented Brazilian table olives.
Methods and Results
Eighteen yeast strains were tested in terms of: safety; survival of ...gastrointestinal and digestion conditions; antimicrobial activity; cellular hydrophobicity; autoaggregation ability and adhesion to epithelial cells; coaggregation and inhibition of pathogenic bacteria adhesion. Six yeasts showed favourable results for all probiotic attributes: Saccharomyces cerevisiae CCMA 1746, Pichia guilliermondii CCMA 1753, Candida orthopsilosis CCMA 1748, Candida tropicalis CCMA 1751, Meyerozyma caribbica CCMA 1758 and Debaryomyces hansenii CCMA 1761. These yeasts demonstrated resistance to 37°C, pH 2·0 and bile salts, and survived in vitro digestion (≥106 CFU per ml). Furthermore, the yeasts exhibited a hydrophobic cell surface (42·5–92·2%), autoaggregation capacity (41·0–91·0%) and adhesion to Caco‐2 (62·0–82·8%) and HT‐29 (57·6–87·3%) epithelial cell lines. Also, the strains showed antimicrobial activity against Salmonella Enteritidis as well as the ability to coaggregate and reduce the adhesion of this pathogen to intestinal cells.
Conclusions
Autochthonous yeasts from naturally fermented Brazilian table olives have probiotic properties, with potential for development of new probiotic food products.
Significance and Impact of Study
These data are important and contribute to the knowledge of new potential probiotic yeasts capable of surviving gastrointestinal tract conditions and inhibiting pathogenic bacteria.
Background
Surgeons in the operating theatre deal constantly with high‐demand tasks that require simultaneous processing of a large amount of information. In certain situations, high cognitive load ...occurs, which may impact negatively on a surgeon's performance. This systematic review aims to provide a comprehensive understanding of the different methods used to assess surgeons' cognitive load, and a critique of the reliability and validity of current assessment metrics.
Methods
A search strategy encompassing MEDLINE, Embase, Web of Science, PsycINFO, ACM Digital Library, IEEE Xplore, PROSPERO and the Cochrane database was developed to identify peer‐reviewed articles published from inception to November 2016. Quality was assessed by using the Medical Education Research Study Quality Instrument (MERSQI). A summary table was created to describe study design, setting, specialty, participants, cognitive load measures and MERSQI score.
Results
Of 391 articles retrieved, 84 met the inclusion criteria, totalling 2053 unique participants. Most studies were carried out in a simulated setting (59 studies, 70 per cent). Sixty studies (71 per cent) used self‐reporting methods, of which the NASA Task Load Index (NASA‐TLX) was the most commonly applied tool (44 studies, 52 per cent). Heart rate variability analysis was the most used real‐time method (11 studies, 13 per cent).
Conclusion
Self‐report instruments are valuable when the aim is to assess the overall cognitive load in different surgical procedures and assess learning curves within competence‐based surgical education. When the aim is to assess cognitive load related to specific operative stages, real‐time tools should be used, as they allow capture of cognitive load fluctuation. A combination of both subjective and objective methods might provide optimal measurement of surgeons' cognition.
Complex field
Abstract Ethanol (EtOH) is a drug widely consumed throughout the world that promotes several neurochemical disorders. Its deleterious effects are generally associated with modifications in oxidative ...stress parameters, signaling transduction pathways, and neurotransmitter systems, leading to distinct behavioral changes. Taurine (2-aminoethanesulfonic acid) is a β-amino acid not incorporated into proteins found in mM range in the central nervous system (CNS). The actions of taurine as an inhibitory neurotransmitter, neuromodulator, and antioxidant make it attractive for studying a potential protective role against EtOH-mediated neurotoxicity. In this study, we investigated whether acute taurine cotreatment or pretreatment (1 h) prevent EtOH-induced changes in acetylcholinesterase (AChE) activity and in oxidative stress parameters in zebrafish brain. The results showed that EtOH exposure (1% in volume) during 1 h increased AChE activity, whereas the cotreatment with 400 mg·L−1 taurine prevented this enhancement. A similar protective effect of 150 and 400 mg·L−1 taurine was also observed when the animals were pretreated with this amino acid. Taurine treatments also prevented the alterations promoted in superoxide dismutase and catalase activities by EtOH, suggesting a modulatory role in enzymatic antioxidant defenses. The pretreatment with 150 and 400 mg·L−1 taurine significantly increased the sulfydryl levels as compared to control and EtOH groups. Moreover, 150 and 400 mg·L−1 taurine significantly decreased thiobarbituric acid reactive species (TBARS) levels, but the cotreatment with EtOH plus 400 mg·L−1 taurine did not prevent the EtOH-induced lipoperoxidation. In contrast, the pretreatment with 150 and 400 mg·L−1 taurine prevented the TBARS increase besides decreased the basal levels of lipid peroxides. Altogether, our data showed for the first time that EtOH induced oxidative stress in adult zebrafish brain and reinforce the idea that this vertebrate is an attractive alternative model to evaluate the beneficial effect of taurine against acute EtOH exposure.
ABSTRACT
In this work we revisit the issue of the rotation speed of the spiral arms and the location of the corotation radius of our Galaxy. This research was performed using homogeneous data set of ...young open clusters (age < 50 Myr) determined from Gaia Data Release 2 (DR2) data. The stellar astrometric membership was determined using proper motions and parallaxes, taking into account the full covariance matrix. The distance, age, reddening, and metallicity of the clusters were determined by our non-subjective multidimensional global optimization tool to fit theoretical isochrones to Gaia DR2 photometric data. The rotation speed of the arms is obtained from the relation between age and angular distance of the birthplace of the clusters to the present-day position of the arms. Using the clusters belonging to the Sagittarius–Carina, Local, and Perseus arms, and adopting the Galactic parameters R0 = 8.3 kpc and V0 = 240 km s−1, we determine a pattern speed of 28.2 ± 2.1 km s−1 kpc−1, with no difference between the arms. This implies that the corotation radius is Rc = 8.51 ± 0.64 kpc, close to the solar Galactic orbit (Rc/R0 = 1.02 ± 0.07).
Summary
The single‐implant mandibular overdenture (SIMO) has been proposed as an alternative for edentulous patients who are poorly adapted to their dentures due to low retention and stability of the ...conventional mandibular complete denture (CD). However, there is a lack of evidence regarding the effectiveness of SIMO, which can be measured by examining patient perception of treatment effects. The aim of this systematic review was to assess the comparative results of CD and SIMO treatments using patient‐reported outcome measures. A literature search was carried out in PubMed, Scopus and Cochrane Central databases. The search included studies published up to July 2017. The focus question was: ‘Do single‐implant mandibular overdentures improve patient‐reported outcomes compared to conventional complete dentures in edentulous patients?’ Eligible studies were randomised clinical trials (RCT) and prospective studies. After initial screening for eligibility and full‐text analysis, 11 studies were included for data extraction and quality assessment (five parallel‐group RCTs, two crossover RCTs and four prospective studies). All studies reported marked improvement in satisfaction with the dentures and quality of life measures after SIMO treatment, irrespective of variations in implant treatment protocols and retention systems. Methodological considerations revealed a lack of evidence from RCTs on the comparative effectiveness of the two treatment strategies. Hence, although available evidence suggests considerable improvement in patient‐reported outcomes following the insertion of a single implant to retain a mandibular denture, further well‐designed comparative studies between SIMO and CD are required to improve the level of evidence and to support the indication of SIMO treatment in routine practice.
AIMS: This study aimed to evaluate the effects of inoculation of strains of lactic acid bacteria (LAB) isolated from sugarcane grown in a Brazil on the quality of corn silage. METHODS AND RESULTS: ...Three strains of Lactobacillus buchneri (UFLA SLM11, UFLA SLM103 and UFLA SLM108), five strains of Lactobacillus plantarum (UFLA SLM08, UFLA SLM41, UFLA SLM45, UFLA SLM46 and UFLA SLM105), and one strain of Leuconostoc mesenteroides (UFLA SLM06) were evaluated at 0, 10, 30, 60 and 90 day after inoculating corn forage. The inoculation of the LAB strains did not influence the chemical composition of the silage, but pH, acetic acid and 1,2‐propanediol were affected by treatment. The silages inoculated with UFLA SLM11 and SLM108 contained the lowest yeast and filamentous fungi counts during fermentation. Bacteria belonging to the Enterobacteriaceae family, Clostridium genus were detected in the silages inoculated with Lact. buchneri UFLA SLM 11, 103 and 108, as shown by DGGE analysis. Silages inoculated with Lact. buchneri UFLA SLM 11 showed higher aerobic stability. CONCLUSIONS: The Lact. buchneri UFLA SLM11 strain was considered promising as a starter culture or inoculant for corn silages. SIGNIFICANCE AND IMPACT OF THE STUDY: The selection of microbial inoculants for each crop promotes improvement of silage quality. Studies on the chemical and microbiological characteristics of silage provide useful information for improving ensiling techniques.
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