Shared Research Facilities "Petrophysics, Geomechanics and Paleomagnetism", created based on actively developing divisions of the Schmidt Institute of Physics of the Earth RAS, has advanced research ...equipment and unique methods, which determines the possibility of solving a wide range of applied and fundamental problems in Earth sciences. The main activities of the Shared Research Facilities "Petrophysics, Geomechanics and Paleomagnetism" are in the field of geomechanical modelling and petrophysics, paleomagnetism, rock magnetism and applied analytical studies of the composition and structure of minerals, rocks and materials. The research results carried out at the Center are used to solve actual problems of prospecting and exploration of oil and gas basins, mineral deposits, tectonics, global geodynamics and tectonophysics. The article presents the scientific equipment of the Center and its key characteristics, describes the methodological and analytical capabilities, and the most significant results of research groups.
Among Oligohymenophorea (Ciliophora, Alveolata) the subclass Peniculia stands as one of the most well-known groups. Frontonia is the largest genus of Peniculia, and its representatives are spread in ...any type of water bodies as well as in soil. At a first glance, Frontonia species exhibit an overall similar morphology, and form a well-recognizable taxon of ciliates. Despite the general morphological homogeneity, the phylogenetic analysis based on the 18S rDNA sequencing showed that Frontonia is a non-monophyletic group. The systematics of this genus should be deeply reviewed, although additional issues complicate the task solving. First, type species of the genus is not yet clearly established, and no type material is available. In this context, the situation of F. vernalis, one of the first Frontonia ever described, is somehow puzzled: the description of this ciliate made by Ehrenberg (in 1833 and 1838) contains several inaccuracies and subsequent misidentifications by other authors occurred. Moreover, the 18S rDNA sequence of a putative F. vernalis is available on GenBank, but no morphological description of the correspondent specimens is provided; thus, in our opinion, it should be only prudently associated with F. vernalis or at least indicated as "F. vernalis".
In the present work, we provide the neotypification of F. vernalis newly found in Italy, presenting its multidisciplinary description and its neotype material. Similarly, we describe a novel species bearing Chlorella-like endosymbionts, Frontonia paravernalis sp. nov., retrieved in two far distant locations (Italy, Russia). A critical discussion on the status of Frontonia taxonomy and phylogeny is also presented, based on the 18S rDNA sequencing of both these two newly collected species and other 14 frontoniids isolated in different parts of the world. Finally, in the present study F. leucas was neotypified and proposed as the type species of the genus.
Green frontoniids form a monophyletic clade of freshwater organisms characterized by having a single contractile vacuole and bearing intracytoplasmatic Chlorella-like symbionts. With the neotypification of F. vernalis and F. leucas a fundamental step in Frontonia systematics was taken, and the bases for further taxonomic studies were laid.
Breast cancer develops upon sequential acquisition of driver mutations in mammary epithelial cells; however, how these mutations collaborate to transform normal cells remains unclear in most cases. ...We aimed to reconstitute this process in a particular case. To this end, we combined the activated form of the PI 3-kinase harboring the H1047R mutation with the inactivation of the histone lysine methyl-transferase KMT2D in the non-tumorigenic human mammary epithelial cell line MCF10A. We found that PI 3-kinase activation promoted cell-cycle progression, especially when growth signals were limiting, as well as cell migration, both in a collective monolayer and as single cells. Furthermore, we showed that KMT2D inactivation had relatively little influence on these processes, except for single-cell migration, which KMT2D inactivation promoted in synergy with PI 3-kinase activation. The combination of these two genetic alterations induced expression of the
gene that encodes a subunit of the Arp2/3 complex. ARPC5L depletion fully abolished the enhanced migration persistence exhibited by double-mutant cells. Our reconstitution approach in MCF10A has thus revealed both the cell function and the single-cell migration, and the underlying Arp2/3-dependent mechanism, which are synergistically regulated when KMT2D inactivation is combined with the activation of the PI 3-kinase.
This paper proposes the method of transition from a 3D model to its ontological representation and describes its usage in the aircraft design process. The problems of design for manufacturability and ...design automation are also discussed. The introduced method is to aim to ease the process of data exchange between important aircraft design phases, namely engineering and design control. The method is also intended to increase design speed and 3D model customizability. This requires careful selection of the complex systems (CAD / CAM / CAE / PDM), providing the basis for the integration of design and technological preparation of production and more fully take into account the characteristics of products and processes for their manufacture. It is important to solve this problem, as investment in the automation define the company's competitiveness in the years ahead.
Various options for designing the direct oxidation of natural gas to methanol are considered: flow-through, with a distributed supply of the oxidizer along the length of the reactor, and circulation. ...For each of the considered options for designing the process, the methanol yield was calculated, as well as the parameters at which the resulting blow-off gas can be used as a fuel. The optimal recycling rate in the circulation process has been determined.
The paper discusses a typical example of recognizing a sheet metal part containing 2 structural elements. According to the developed recognition algorithm, a software module was implemented for ...automated recognition of structural elements in a 3-dimensional model of parts with the * .stp extension, which allowed working not only with models made in the Siemens NX system, but also, in general, with almost any 3D models exported in this format. The result of recognition can be used to develop systems that design technological processes for the production of mechanical engineering products, including at aircraft production enterprises.
BACKGROUND: Flagellates of the family Trypanosomatidae are obligate endoparasites, which can be found in various hosts. Several genera infect insects and occur as monoxenous parasites especially in ...representatives of Diptera and Hemiptera. These trypanosomatid flagellates probably share the worldwide distribution of their hosts, which are often infested by large numbers of endoparasites. Traditionally, their taxonomy was based on morphology, host origin, and life cycle. Here we report the characterization of a trypanosomatid infection detected in a protozoan, a ciliate collected from a polluted freshwater pond in a suburb of New Delhi (India). METHODS: Live observations and morphological studies applying light, fluorescence and transmission electron microscopy were conducted. Molecular analyses of host and parasite were performed and used for phylogenetic reconstructions and species (host) or genus level (parasite) identification. RESULTS: Although the morphological characteristics were not revealing, a high similarity of the trypanosomatids 18S rRNA gene sequence to Herpetomonas ztiplika and Herpetomonas trimorpha (Kinetoplastida, Trypanosomatidae), both parasites of biting midges (Culicoides kibunensis and Culicoides truncorum, respectively) allowed the assignment to this genus. The majority of the host population displayed a heavy infection that significantly affected the shape of the host macronucleus, which was the main site of parasite localization. In addition, the growth rate of host cultures, identified as Euplotes encysticus according to cell morphology and 18S rRNA gene sequence, was severely impacted by the infection. CONCLUSIONS: The host-parasite system described here represents a recent example of free-living protists acting as environmental reservoirs for parasitic eukaryotic microorganisms.
The research refers to the field of automation of technological preparation of production in mechanical engineering. There is a concept of technological operations selection at manufacturing of a ...standard component of an airframe of an airplane glider on the basis an image of a product and objects' classifiers of an industrial environment. Presentation of the objects of the production environment and their communication are performed in the presented theoretical and multiplicity model. The development of technological processes is considered; 3D models of the part are used in the process of TP design. The concept of the technique of automated construction of the manufacturing route on the basis of dependencies between the objects of the production environment "KE-TO-STO-Equipment" is presented.
The method proposed by the authors makes it possible to determine the nature of changes in two components (intergranular and fracture) of the total porosity. Changes in the fracture and intergranular ...porosity were investigated during fracture nucleation of sandstone samples under modeling in situ conditions. Sandstone samples were held at constant confining and pore pressure modeling in situ conditions. At the same time, only a slight decrease in the total and intergranular porosity was observed. However, only in the sample with an initially large fracture porosity was an increase in fracture porosity observed. Growth of additional axial compression reduced all types of porosity. Fracture preparation of the sample with an initially small fracture porosity was predominantly accompanied by a decrease in total and intergranular porosity and increase in fracture porosity, i.e., growth of microcracks. At the same time, during fracture preparation of a sample with an initially large value fracture porosity, an increase in total and intergranular porosity and decrease in fracture porosity were observed, which is characteristic of dilatancy processes. Differences in the fracture nucleation of sandstone samples were revealed, which indicates that their initial fracture porosity has a significant effect on them.
Since the beginning of the 1980s, the Paramecium–Holospora system has become an important model and a subject of international scientific cooperation in symbiosis studies, established by Prof. H.-D. ...Görtz. In the article, a brief sketch of the scientist's life, his areas of interest, expertise and his contribution to our knowledge of protozoan symbiosis are discussed.
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