1. Cytochrome P-450-linked monooxygenase of mitochondria and microsomes of the cerebrum, cerebellum and pituitary gland of bovine brain were investigated biochemically and immunochemically. 2. The ...cytochromes P-450scc and P-450(11) beta, NADPH-ferredoxin reductase and adreno-ferredoxin were detected and their enzymatic activities of steroid hydroxylations were found in the bovine brain.
The purpose of this study was to evaluate bacterial infection in bile juice in 18 cases undergoing biliary tract operation. Bile juice was taken from a drainage tube of the biliary tract during the ...operation procedure and as time elapsed after the operation, and was cultured to define the bacterial infection. The bile juice was highly contaminated with bacteria during examination, in which enterococcus and Gram-negative rod were frequently detected. The incidence of infection with enterococcus, E. coli or Klebsiella tended to increase after the operation, taking part in plural bacterial infection. Infection of bile juice was closely related with obstruction of the bile tract before operation and operative procedures. No preservation of papilla Vater function and plural bacterial infection, including anaerobic bacterial infection, might participate in the occurrence of biliary tract infection after the operation. Therefore, it was suggested that different preventive measure was necessary based on the degree of bacterial infection in each case.
Foliar applied plant growth regulators, uniconazole (S-07) and paclobutrazol (PP 333), promoted the formation of flower bud in tea plants. Optimum concentrations for the formation of flower bud were ...from 25 to 100 ppm in S-07, and from 500 to 2, 000 ppm in PP 333. Effective times of application were in the middle of May to the first decade of July. In case of the applications before the middle of June, time of full bloom was early in October, which was same as no treatment. When both chemicals were applied on June 25 and July 10, full blooms were observed in late October and in early November, respectively. Elongation of new shoots was suppressed by the chemicals. The higher the concentration of the chemicals and the later the time of application became, the less the new shoots grew until late autumn. Application of S-07 at 150 ppm, and PP 333 at more than 4, 000 ppm markedly delayed the bud opening in the next spring.
In previous papers, the authors reported that gamma -glutamyltranspeptidase activity mainly participates in the biosynthesis of polyglutamate (PGA) in Bacillus subtilis (natto ) Asahikawa, isolated ...from the commercial product, "natto," and that the function of the 5.7-kilobase plasmid isolated from the bacteria is in PGA production. In order to understand the role of the plasmid in PGA production, the authors further characterized the plasmid. This paper presents the restriction endonuclease cleavage site map of the plasmid, pUH1.
Examination of pubescence of young leaf was made on tea clones collected from China (270 clones), Japan (1, 241 clones), Taiwan (73 clones), India (567 clones) and other tea producing countries (295 ...clones). Twentythree patterns were classified based on width, length, density and distribution of leaf hair. As a whole, China tea clones belonging to var. sinesis showed narrower variation than Assam tea clones belonging to var, assamica. China tea clones had long hairs of high density distribution on entire surface of leaf. On the other hand, Assam tea clones had short hairs and medium/low density with limited distribution on the inner part of leaf lamina near to midrib. Only tea clones from Darjeeling in India, belonging to var. sinensis according to their small leaf, did not show the typical pattern of pubescence similar to China tea clones. This would suggest some influence by var. assamica on their pubescence pattern. Taiwan tea clones (Taiwan-Yamacha) had absolutely no hair on their young leaves. At present, Taiwan tea clones are included in var. assamica. But their pubescence patterns were revealed great difference with true pattern of var. assamica.
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