Concentrations of heavy metals (Cu, Zn, Cd, Pb and As) were measured in water, sediment and two fish species,
Sparus aurata and
Solea senegalensis, from the estuary of Tinto and Odiel rivers in ...Huelva (Spain), one of the most metallic polluted estuaries in Europe.
As a forward step to understand metal bioavailability and assess the potential impact on aquatic biota, a study of heavy metal speciation of sediments and water were achieved. High levels of total and dissolved Zn and Cu were found in water and high pollution of Zn, Pb, As and Cu were found in sediments. Availability of metals was established as following ranking: Cd
>
Zn
>
Cu
>
Pb in both water and sediment. In addition, the effect of this pollution was studied by evaluation of metal bioaccumulations and correlations obtained between metal levels in fractions of water and sediment and metal levels in fish tissues. High Cu and Zn levels were observed in liver tissue of both species, in according with higher total content and more available metals in water and sediment. Correlations among metal content in tissues and different fractions of metal in water for
S. aurata and sediment for
S. senegalensis were found.
A study to determine total and mobile heavy metals concentrations in sediments from Algeciras Bay was performed and pollution hotspots were identified. The effects on aquatic organisms were ...established using sediment quality guidelines (SQGs). Ni and Cr exceeded the effect range medium and low levels, respectively, around industrial area. Potential toxicity of metals was determined by diethylenetriaminepenta-acetic acid (DTPA) extractions and low alert levels of Co, Cu, Zn, Cd, Ni and Pb were exceeded at most sampling sites. Three pollution indicators were used showing significant values for As, Ni, Cr, Pb and Cd. Sediment speciation using the sequential extraction BCR procedure was carried out, being Cd, Zn, Pb and As the most available metals. Principal component, cluster and ANOVA analyses were performed in order to assess the sources of metals and the influence of seasonality and anthropogenic activities on the sediment quality. Two principal component analysis (PCA) factors were obtained identifying the sampling sites affected by anthropogenic activities; Ni–Cr and Zn–Cu–V clusters were also obtained associated with stainless steel and petrochemical industrial activities. ANOVA showed the outstanding sites because of total metal concentration and significant differences among sampling sites by the acid extractable and reducible fractions for all metals except for Ba and V.
Pruning residues can provide a significant amount of biomass despite being rarely used as a renewable sources to replace fossil fuels. Exploiting such residues entails creating a sustainable and ...cost-effective supply chain in which the harvesting and initial processing of the residues play a crucial role. The study is a detailed and accurate survey of the harvesting technologies available in Europe for harvest pruning. After defining the main harvest technologies and the distribution of manufacturers in Europe, the survey details the main groups of implements: shredders, chippers and balers. For each group, the most important configurations are discussed, together with the main characteristics of the machine. Some of the main innovations are detailed (modular machines, non-stop balers, densification of the biomass) which can improve the quality of the product and the economic sustainability of the chain.
•The main balers, shredders and chippers devoted to pruning collection were reviewed.•Eighteen type of harvesting systems were classified and described.•Among the seventy-five models of implements identified, chippers resulted predominant.•The review discussed the main technical efforts required to further optimize the collection.
•Intracranial artery dissection is a non-atherosclerotic rare cause of stroke.•Frequent in the middle cerebral artery and supraclinoid portion of internal carotid.•Radiology findings: Intimal flap, ...double lumen, intramural hematoma, pseudo-aneurysma.•Hypertension, connective tissue disease and migraine are important risk factors.•Therapy with antiplatelets or anticoagulants is still the mainstay of treatment.
Intracranial artery dissection (IAD) is an underdiagnosed, non-atherosclerotic cause of stroke with various clinical manifestations. To identify all the potential studies investigating the epidemiology, risk factors, symptoms, radiology findings, and treatment methods of IAD, we conducted a literature search screening PubMed, SCOPUS, EMBASE, and BIREME. According to the results of several studies, IAD is the major cause of ischemic stroke in at least one-third of the cervical-cranial artery dissection (CCAD) cases presenting with ischemic stroke. Mechanical causes are associated with cervical artery dissections (CAD) in up to 40 % of the cases. However, the risk factors for IAD are still not completely understood. Antithrombotic therapy with either antiplatelet or classic anticoagulants is the mainstay of treatment for preventing further thromboembolic complication after a stroke. Endovascular or surgical treatment options can be considered when medical therapies are not effective or when there is a high rate of recurrence or increased risk of bleeding. The observational studies have shown that these methods are very effective in preventing recurrence and significantly improving morbidity and mortality in patients with ruptured dissections. Clinical trials are required to establish the best option for each mechanism of ischemic lesion.
The genus Mazama stands out among the Neotropical deer due to their wide intra and interspecific karyotypic diversification, which is associated with an accentuated chromosomal fragility. There are ...reports of heterozygous Robertsonian translocation (RT) carriers in a free-range population of Mazama gouazoubira (brown brocket deer), as well as in captive animals of this and other species of the genus. To analyze possible negative impacts of heterozygous chromosome rearrangements on reproductive fitness of the carriers, we performed an analysis of sperm meiotic segregation in four brown brocket bucks, carriers of a rob(4;16), and compared the results with those of a normal buck. We established a reliable FISH and sperm-FISH protocol for the brown brocket deer using bovine (Bos taurus; diploid number, 2n = 60) whole chromosome painting (WCP) and BAC probes. Using BAC probes, we revealed the presence of a paracentric inversion (PAI) of the fused chromosome 4 in two of the four analyzed RT carriers. The mean frequency of normal/balanced sperm in the translocation carriers was significantly lower than in the normal buck (94.78% vs 98.40%). The mean value of total unbalanced spermatozoa was almost doubled in the RT/PAI carriers (6.68%) when compared to RT carriers (3.76%), but the difference was not statistically significant. This study demonstrated the efficiency of FISH with bovine WCP and BAC probes in the characterization of chromosome rearrangements and gametic segregation patterns in brown brocket deer. Our results indicate a low to moderate increase in the rates of unbalanced meiotic segregation products in brown brocket bucks heterozygous for RT and RT/PAIs.
•Presence of heterozygous rob(4;16) and rob(4;16)inv(4) in M. gouazoubira.•First report of FISH on M. gouazoubira sperm nuclei using bovine BAC probes.•Low to moderate rates of unbalanced meiotic segregation products of the carriers.
Key Takeaways
A new study of phytoplankton in the Detroit River follows significant changes to ecological and environmental conditions in the boundary waters and updates historical data research.
...Measures to remediate river health in recent years can be evaluated through analysis of the abundance and diversity of phytoplankton communities at a higher taxonomic resolution.
By working with community partners to measure phytoplankton communities in the long term, water treatment plants can use the data gained to design an early‐warning system for river changes.
There are 8 million people with Chagas disease worldwide and in El Salvador approximately 39% of the population is at risk of contracting the disease. One of the principal challenges in mitigating ...Chagas is evaluating the role of the vector ecology of triatomine species in the transmission of the Trypanosoma cruzi parasite in anthropogenically modified habitats, where new patterns of transmission frequently arise. Field studies of triatomine vector ecology in El Salvador have largely focused on describing parameters that contribute to infestation patterns, which may themselves be rooted in the morphological variability that exists in triatomine populations. The objective of this study was to evaluate the morphology of the vector species Triatoma dimidiata with respect to the characteristics of the ecological landscape the vector inhabits throughout El Salvador. We used image analyses to evaluate T. dimidiata morphological variability and then used Geographic Information Systems to intersect the morphological point-data with map layers containing different environmental characteristics. Our study found that the variation in the size, shape, and coloration of T. dimidiata varied in relation to elevation, Holdridge life zone, soil type and land use. We further characterize the local morphological adaptations of T. dimidiata with respect to the local ecological, biological, and geographical conditions in El Salvador. We suggest that future studies consider a molecular exploration of local T. dimidiata species complex in El Salvador, especially since morphological studies of triatomine species complex have found that variability correlate with the genetic variability of the population.
In this paper, breast cancer patients were monitored throughout their chemotherapy treatments (CHT), with blood serum sample Raman spectroscopy and multivariate analysis, approximately for a year. ...First of all, we discriminate between healthy and clinically diagnosed breast cancer patients. Breast cancer detection in terms of sensitivity and specificity were 87.14
%
and 90.55
%
respectively. Although no shifts of peaks in mean spectrum of samples from breast cancer patients were found with respect to the mean spectrum from control patients, some peaks did show clear differences in intensity, the greatest disparities found at 509, 545, 1063, 1103, 1338, 1556, 1083 and 1449 cm
− 1
are associated with amino acids and phospholipid, 1246 and 1654 cm
− 1
, corresponding to amide III and I, respectively. Other peaks of interest encountered at 450, 661, 890, 917 and 1405 cm
− 1
are associated to glutathione. Then, 6 breast cancer patients were monitored during their chemotherapy treatments, the results were in complete correspondence with their medical records, enabling a detailed study of the evolution of each patient’s cancer. A special interest arose in the possible correlation between the intensity of Raman peak, 450 cm
− 1
, corresponding to glutathione and evolution of cancer throughout CHT, i.e., glutathione appears to be a good candidate as breast cancer biomarker. The results confirmed that Raman spectroscopy and PCA are, not only a good support to current breast cancer detection techniques, but could also be excellent techniques to monitor more efficiently breast cancer patients undergoing CHT, using blood serum samples which are a lot less invasive than other methods.
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