Background and Objective
Although surveys in the USA have shown that male subjects are more prone to develop periodontitis, sex as a risk factor in periodontitis, and its mechanism, remain ...controversial. Animal models are ideal for investigating immunological mechanisms of sex dimorphism in periodontitis because in these models it is possible to exclude the interference of gender‐related risk factors, such as smoking and oral hygiene habits. Based on surveys in humans and reports on sex dimorphism in other diseases, our hypothesis is that sex is a risk factor in periodontitis.
Material and Methods
Different murine models (oral gavage model and ligature model) for periodontitis have been utilized to determine susceptibility to periodontitis in female and male mice. Periodontal bone levels were measured as the distance from the cemento–enamel junction to the alveolar bone crest (CEJ‐ABC) in young female or male mice (8–10 wk of age). Differential expression of inflammatory mediators in the gingivae of female and male mice was determined by quantitative real‐time PCR.
Results
In comparison with male mice, female mice displayed significantly (p < 0.05) increased periodontal bone loss, accompanied by elevated expression of proinflammatory cytokines (interleukin‐1β, interleukin‐6 and interleukin‐17A) and higher numbers of oral bacteria.
Conclusion
In contrast to the results in humans, in which periodontitis susceptibility is also influenced by confounding gender‐related behaviors, in the murine oral gavage model and ligature model, female mice appear to be more susceptible to periodontal bone loss than male mice. In the ligature model, we observed significantly (p < 0.05) higher CEJ‐ABC distance, gingival proinflammatory cytokine production and number of oral bacteria in female mice. Furthermore, our results imply that female mice develop periodontitis with a higher progression rate. Our study has therefore established that animal models can be used to dissect the mechanisms underlying genuine gender‐based differences in periodontal disease susceptibility and/or progression.
Since the appearance of stony coral tissue loss disease (SCTLD) on reefs off Miami in 2014, this unprecedented outbreak has spread across the entirety of Florida’s coral reef tract, as well as to ...many territories throughout the Caribbean. The endemic zone reached the upper Florida Keys by 2016, resulting in partial or complete mortality of coral colonies across numerous species. Disease was first observed at Cheeca Rocks (Islamorada, Florida) in the beginning of 2018, with reports of coral mortality peaking mid-year. The disease was still present at Cheeca Rocks as of March 2020, however, to a lesser degree compared to the initial outbreak. Annual monitoring efforts have been ongoing at Cheeca Rocks since 2012, including repeated benthic photomosaics of a 330 m
2
survey zone, spanning six replicate sites. As such, a repository of coral community composition data exists for before and after the disease outbreak that was analyzed to assess the impacts of SCTLD on reef communities at an upper Florida Keys inshore reef. Cheeca Rocks is hypothesized to be a resilient reef due to its persistent high coral cover despite its inshore location, which subjects corals to fluctuating water quality and marginal environmental conditions. Coral populations here have been shown to recover from bleaching events and heat stress with minimal coral mortality. Though colonies of coral species characterized as highly and moderately susceptible to SCTLD (e.g.,
Colpophyllia natans
,
Diploria labyrinthiformis
,
Pseudodiploria strigosa
,
Orbicella annularis
, and
O. faveolata
) suffered mortality as a result of the outbreak with an average loss of 16.42% relative cover by species, the overall impacts on coral cover and community structure were relatively low, contributing to a loss of total coral cover of only 1.65%. Comparison of photomosaic data to other studies indicate Cheeca Rocks may not have been affected as severely as other sites on Florida’s coral reef tract, underlying this site’s potential role in coral resilience to stressors including bleaching events, land-based pollution, and disease epizootics.
The Kronos Early Estrogen Prevention Study (KEEPS) was a randomized, double-blind, placebo-controlled trial designed to determine the effects of hormone treatments (menopausal hormone treatments ...MHTs) on the progression of carotid intima-medial thickness (CIMT) in recently menopausal women. Participants less than 3 years from menopause and without a history of overt cardiovascular disease (CVD), defined as no clinical CVD events and coronary artery calcium < 50 Agatston units, received either oral conjugated equine estrogens (0.45 mg/day) or transdermal 17β-estradiol (50 µg/day), both with progesterone (200 mg/day for 12 days/month), or placebo pills and patches for 4 years. Although MHT did not decrease the age-related increase in CIMT, KEEPS provided other important insights about MHT effects. Both MHTs versus placebo reduced the severity of menopausal symptoms and maintained bone density, but differed in efficacy regarding mood/anxiety, sleep, sexual function, and deposition of β-amyloid in the brain. Additionally, genetic variants in enzymes for metabolism and uptake of estrogen affected the efficacy of MHT for some aspects of symptom relief. KEEPS provides important information for use of MHT in clinical practice, including type, dose, and mode of delivery of MHT recently after menopause, and how genetic variants in hormone metabolism may affect MHT efficacy on specific outcomes.
Opiates act on the dopaminergic system of the brain and perturb 32 kDa dopamine and adenosine 3′, 5′-monophosphate-regulated phosphoprotein (DARPP-32) function. The DARPP-32 mediated inhibition of ...protein phosphatase-1 (PP-1) and modulation of transcriptional factor CREB is critical to the changes in neuronal plasticity that result in behavioral responses during drug abuse. To investigate the role of DARPP-32 mediated signaling on withdrawal behavior in a rat model of opiate addiction, we used intracerebral administration of gold nanorods (GNR) complexed to DARPP-32 siRNA to silence DARPP-32 gene expression and measure its effects on the opiate withdrawal syndrome. We hypothesized that DARPP-32 siRNA will suppress the neurochemical changes underlying the withdrawal syndrome and therefore prevent conditioned place aversion by suppressing or removing the constellation of negative effects associated with withdrawal, during the conditioning procedure. Our results showed that opiate addicted animals treated with GNR-DARPP-32 siRNA nanoplex showed lack of condition place aversive behavior consequent to the downregulation of secondary effectors such as PP-1 and CREB which modify transcriptional gene regulation and consequently neuronal plasticity. Thus, nanotechnology based delivery systems could allow sustained knockdown of DARPP-32 gene expression which could be developed into a therapeutic intervention for treating drug addiction by altering reward and motivational systems and interfere with conditioned responses.
A body of literature has emerged suggesting that investors herd, or tend to make investment decisions on the basis of information provided by the trades of other market participants. In this paper, ...we use intraday data to examine whether traders herd during periods of extreme market movements using sector Exchange Traded Funds (ETFs). Two procedures, one based on identifying extreme up market and down market periods and the other based on incorporating a nonlinear term in a regression specification, are used to identify the possibility of the existence of herding behavior in nine sector ETFs traded on the American Stock Exchange. The results support the conclusion that investors do not herd during periods of extreme market movements using ETFs. Furthermore, we show that the market reaction to news is not symmetric for up markets and down markets.
Methods 131 children (67 Caucasians, 37 Hispanics, 27 AA's) with moderate asthma had exhaled nitric oxide (FeNO) and spirometry measured before, during and after an acute asthma exacerbation ...(following a course of prednisone).
Asthma is a significant health problem among children: 9.3% of children in the United States suffer from asthma. Children with persistent asthma in inner cities have increased health care ...utilization, worse health care outcomes, increased school absences, and worse academic performance.
We sought to create and evaluate a school-centered asthma program to reduce asthma morbidity and create asthma-friendly schools.
We developed, implemented, and evaluated the Step-Up Asthma Program, a multidisciplinary school-centered asthma program. The program was designed as an outreach program with asthma counselors as a bridge between subspecialty asthma care, primary care providers, school nurses, and children with asthma. The core components of the program involve identifying children with asthma, providing evidence-based asthma education, and case management. Students' asthma knowledge, inhaler technique, and number of asthma exacerbations were evaluated over a 2-year period (2010-2012) as a pre-post study.
A total of 252 students enrolled in the Step-Up Asthma Program over a 2-year period. Significant improvements were noted in number of asthma action plans, rescue medications at school, and asthma controllers. Program participants had significant improvements in asthma knowledge scores (P < .001) and inhaler technique (P < .0001). There were significant reductions in asthma exacerbations defined as oral steroid courses, urgent care visits, and missed school days (P < .05) that persisted over time.
A guideline-based school-centered asthma program can significantly reduce asthma morbidity. The asthma counselor is the cornerstone of the program, providing asthma education and care coordination. The Step-Up Asthma Program is in its 10th year, and we believe the key elements of this program can be implemented in other school systems.
With compelling evidence that half the world’s coral reefs have been lost over the last four decades, there is urgent motivation to understand where reefs are located and their health. Without such ...basic baseline information, it is challenging to mount a response to the reef crisis on the global scale at which it is occurring. To combat this lack of baseline data, the Khaled bin Sultan Living Oceans Foundation embarked on a 10-yr survey of a broad selection of Earth’s remotest reef sites—the Global Reef Expedition. This paper focuses on one output of this expedition, which is meter-resolution seafloor habitat and bathymetry maps developed from DigitalGlobe satellite imagery and calibrated by field observations. Distributed on an equatorial transect across 11 countries, these maps cover 65,000 sq. km of shallow-water reef-dominated habitat. The study represents an order of magnitude greater area than has been mapped previously at high resolution. We present a workflow demonstrating that DigitalGlobe imagery can be processed to useful products for reef conservation at regional to global scale. We further emphasize that the performance of our mapping workflow does not deteriorate with increasing size of the site mapped. Whereas our workflow can produce regional-scale benthic habitat maps for the morphologically diverse reefs of the Pacific and Indian oceans, as well as the more depauperate reefs of the Atlantic, accuracies are substantially higher for the former than the latter. It is our hope that the map products delivered to the community by the Living Oceans Foundation will be utilized for conservation and act to catalyze new initiatives to chart the status of coral reefs globally.
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