We report on 318 patients with acute leukemia, receiving donor lymphocyte infusion (DLI) in complete hematologic remission (CHR) after allogeneic stem cell transplantation (alloSCT). DLI were applied ...preemptively (preDLI) for minimal residual disease (MRD, n = 23) or mixed chimerism (MC, n = 169), or as prophylaxis in high-risk patients with complete chimerism and molecular remission (proDLI, n = 126). Median interval from alloSCT to DLI1 was 176 days, median follow-up was 7.0 years. Five-year cumulative relapse incidence (CRI), non-relapse mortality (NRM), leukemia-free and overall survival (LFS/OS) of the entire cohort were 29.1%, 12.7%, 58.2%, and 64.3%. Cumulative incidences of acute graft-versus-host disease (aGvHD) grade II-IV°/chronic GvHD were 11.9%/31%. Nineteen patients (6%) died from DLI-induced GvHD. Age ≥60 years (p = 0.046), advanced stage at transplantation (p = 0.003), shorter interval from transplantation (p = 0.018), and prior aGvHD ≥II° (p = 0.036) were risk factors for DLI-induced GvHD. GvHD did not influence CRI, but was associated with NRM and lower LFS/OS. Efficacy of preDLI was demonstrated by decreasing MRD/increasing blood counts in 71%, and increasing chimerism in 70%. Five-year OS after preDLI for MRD/MC was 51%/68% among responders, and 37% among non-responders. The study describes response and outcome of DLI in CHR and helps to identify candidates without increased risk of severe GvHD.
Recently, oversulfated chondroitin sulfate (OSCS) was identified in contaminated heparin preparations, which were linked to several adverse clinical events and deaths. Orthogonal analytical ...techniques, namely nuclear magnetic resonance (NMR) and capillary electrophoresis (CE), have since been applied by several authors for the evaluation of heparin purity and safety. NMR identification and quantification of residual solvents and non-volatile low molecular contaminants with USP acceptance levels of toxicity was achieved 40-fold faster than the traditional GC-headspace technique, which takes ~120 min against ~3 min to obtain a (1)H NMR spectrum with a signal/noise ratio of at least 1000/1. The procedure allowed detection of Class 1 residual solvents at 2 ppm and quantification was possible above 10 ppm. 2D NMR techniques (edited-HSQC (1)H/(13)C) permitted visualization of otherwise masked EDTA signals at 3.68/59.7 ppm and 3.34/53.5 ppm, which may be overlapping mononuclear heparin signals, or those of ethanol and methanol. Detailed NMR and ESI-MS/MS studies revealed a hitherto unknown contaminant, tris(2-n-butoxyethyl) phosphate (TBEP), which has potential health risks.
► P. pulmonarius was grown in submerged cultured using different carbon sources. ► The mycelia yield varied depending on the culture medium carbon source. ► Samples grown with xylose and arabinose ...had high protein content of above than 19%. ► All cultured biomass showed to be composed mainly of unsaturated fatty acids. ► Exopolysaccharides fractions showed as main structures glucans and mannogalactans.
For many years mushrooms have been consumed and appreciated by their nutritional value, and medicinal properties. The traditional mushroom cultivation takes too long and the macrofungi biotechnology has not been explored in its full potential yet. The goal of this work was to observe if different carbon sources could improve the yield and diversify fungi nutrient composition in submerged culture.
Pleurotus pulmonarius mycelia and exopolysacharide productions were evaluated using glucose, galactose, xylose and arabinose. The mycelia yield varied depending on the culture medium, and galactose showed to be the best carbon source to produce EPS. Samples that showed the highest protein contents were grown with xylose (19.44%) and arabinose (26.05%). Furthermore, the biomass cultivated with these carbohydrates and with galactose showed five essential amino acids. All cultured biomass showed low lipid contents (∼1%), being composed mainly of unsaturated fatty acids. All EPS fractions showed as main structures glucans and mannogalactans.
Prostate-specific membrane antigen (PSMA) has been explored as a target for molecular imaging of prostate cancer and other malignancies that express PSMA in their tumor-associated neovasculature. ...Although several PSMA-targeted radiotracers labeled with a variety of radionuclides have been reported, positron-emitting radiotracers labeled with
F are of particular interest. One such compound, the small molecule PSMA inhibitor
FDCFPyL, has demonstrated initial success. This article reviews the literature on this radiotracer, including radiosynthetic approaches to the molecule, data that are available from preclinical experiments, and evidence to date of the clinical utility of this agent in prostate cancer and clear cell renal cell carcinoma.
Abstract A precise measurement of the vector and axial-vector form factors difference $$F_V-F_A$$ FV-FA in the $$K^+\rightarrow {\mu ^+}{\nu _{\mu }}{\gamma }$$ K+→μ+νμγ decay is presented. About 95K ...events of $$K^+\rightarrow {\mu ^+}{\nu _{\mu }}{\gamma }$$ K+→μ+νμγ are selected in the OKA experiment. The result is $$F_V-F_A=0.134\pm 0.021(stat)\pm 0.027(syst)$$ FV-FA=0.134±0.021(stat)±0.027(syst) . Both errors are smaller than in the previous $$F_V-F_A$$ FV-FA measurements.
Age-related macular degeneration (AMD) is the leading cause of blindness in ageing societies, triggered by both environmental and genetic factors. The strongest genetic signal for AMD with odds ...ratios of up to 2.8 per adverse allele was found previously over a chromosomal region in 10q26 harboring two genes, ARMS2 and HTRA1, although with little knowledge as to which gene or genetic variation is functionally relevant to AMD pathology. In this study, we analyzed rare recombinant haplotypes in 16,144 AMD cases and 17,832 controls from the International AMD Genomics Consortium and identified variants in ARMS2 but not HTRA1 to exclusively carry the AMD risk with P-values between 1.0 × 10
and 6.7 × 10
This now allows prioritization of the gene of interest for subsequent functional studies.
Summary
Introduction
Digital tools are widely used and effective in weight management interventions; however, usage declines over time. Strategies to promote continued engagement should be explored. ...We examined the effects of offering additional modes of weight reporting as well as periodic online campaigns to promote engagement, assessed by frequency of weight reporting, in a weight gain prevention study for young adults.
Methods
Using an observational design, self‐reported weights obtained through digital tools were pooled across participants assigned to two interventions (
n
= 312). Analysis examined the effects before during and after introduction of an additional reporting modality (email) and for three time‐limited refresher campaigns over 2 years.
Results
Adding a new modality to the three existing modes (SMS, web, and mobile web) increased weight reporting as well as the number of modalities participants used to report weights. The use of several modes of reporting was associated with more weights submitted (
p
< 0.01). Refresher campaigns did not increase the proportion of participants reporting; however, the number of weights submitted during the 4‐week campaigns increased compared with the 4 weeks before the campaign (
p
's ≥ 0.45, <0.001, respectively).
Conclusion
Using multiple digital modalities and periodic campaigns shows promise for sustaining engagement with weight reporting in a young adult population, and incorporating such strategies may mitigate typical declines in eHealth and mHealth interventions.
Study of the decay K+→π+π-π+γ in the OKA experiment Shapkin, M. M.; Akimenko, S. A.; Artamonov, A. V. ...
The European physical journal. C, Particles and fields,
04/2019, Volume:
79, Issue:
4
Journal Article
Peer reviewed
Open access
A high statistics data sample of the decays of
K
+
mesons to three charged particles was accumulated by the OKA experiment in 2012 and 2013. This allowed to select a clean sample of about 450 events ...with
K
+
→
π
+
π
-
π
+
γ
decays with the energy of the photon in the kaon rest frame greater than 30 MeV. The measured branching fraction of the
K
+
→
π
+
π
-
π
+
γ
, with
E
γ
∗
>
30
MeV
is equal to
(
0.71
±
0.05
)
×
10
-
5
. The measured differential branching fraction over photon energy is compared with the prediction of the chiral perturbation theory to
O
(
p
4
)
. A search for an up-down asymmetry of the photon with respect to the hadronic system decay plane is also performed.
A precise measurement of the vector and axial-vector form factors difference
F
V
-
F
A
in the
K
+
→
μ
+
ν
μ
γ
decay is presented. About 95K events of
K
+
→
μ
+
ν
μ
γ
are selected in the OKA ...experiment. The result is
F
V
-
F
A
=
0.134
±
0.021
(
s
t
a
t
)
±
0.027
(
s
y
s
t
)
. Both errors are smaller than in the previous
F
V
-
F
A
measurements.
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