Meat quality is an important trait for pig-breeding programs aiming to meet consumers' demands. Geneticists must improve meat quality based on their understanding of the underlying genetic ...mechanisms. Previous studies showed that most meat-quality indicators were low-to-moderate heritability traits; therefore, improving meat quality using conventional techniques remains a challenge. Here, we performed a genome-wide association study of meat-quality traits using the GeneSeek Porcine SNP50K BeadChip in 582 crossbred Duroc × (Landrace × Yorkshire) commercial pigs (249 males and 333 females). Meat conductivity, marbling score, moisture, meat color, pH, and intramuscular fat (IMF) content were investigated. The genome-wide association study was performed using both fixed and random model Circulating Probability Unification (FarmCPU) and a mixed linear model (MLM) with the rMVP software. The genomic heritability of the studied traits ranged from 0.13 ± 0.07 to 0.55 ± 0.08 for conductivity and meat color, respectively. Thirty-two single-nucleotide polymorphisms (SNPs) were identified for meat quality in the crossbred pigs using both FarmCPU and MLM. Among the detected SNPs, five, nine, seven, four, six, and five were significantly associated with conductivity, IMF, marbling score, meat color, moisture, and pH, respectively. Several candidate genes for meat quality were identified in the detected genomic regions. These findings will contribute to the ongoing improvement of meat quality, meeting consumer demands and improving the economic outlook for the swine industry.
Enzyme immobilization is essential to the commercial viability of various critical large‐scale biocatalytic processes. However, challenges remain for the immobilization systems, such as difficulties ...in loading large enzymes, enzyme leaching, and limitations for large‐scale fabrication. Herein, we describe a green and scalable strategy to prepare high‐performance biocatalysts through in situ assembly of enzymes with covalent organic frameworks (COFs) under ambient conditions (aqueous solution and room temperature). The obtained biocatalysts have exceptional reusability and stability and serve as efficient biocatalysts for important industrial reactions that cannot be efficiently catalyzed by free enzymes or traditional enzyme immobilization systems. Notably, this versatile enzyme immobilization platform is applicable to various COFs and enzymes. The reactions in an aqueous solution occurred within a short timeframe (ca. 10–30 min) and could be scaled up readily (ca. 2.3 g per reaction).
In situ assembly of enzymes and covalent organic frameworks (COFs) enables the environmentally benign large‐scale fabrication of a new generation of high‐performance biocatalysts. This approach shows how limitations in enzyme immobilization can be overcome and it opens up a new avenue for the scalable fabrication of high‐performance biocatalysts to accelerate enzyme industrialization.
Rapid, efficient, and selective removal of toxicants such as aristolochic acid I (AAI) from complex natural product systems is of great significance for the safe use of herbal medicines or ...medicine-food plants. Addressing this challenge, we develop a high-performance separation approach based on ionic covalent organic frameworks (iCOFs) to separate and remove AAI. Two vinylene-linked iCOFs (NKCOF-46-Br- and NKCOF-55-Br-) with high crystallinity are fabricated in a green and scalable fashion via a melt polymerization synthesis method. The resulting materials exhibit a uniform morphology, high stability, fast equilibrium time, and superior affinity and selectivity for AAI. Compared to conventional separation media, NKCOF-46-Br- and NKCOF-55-Br- achieve the record high adsorption capacities of 246.0 mg g−1 and 178.4 mg g−1, respectively. Various investigations reveal that the positively charged framework and favorable pore microenvironment of iCOFs contribute to their high selectivity and adsorption efficiency. Moreover, the iCOFs exhibit excellent biocompatibility by in vivo toxicity assays. This study paves a new avenue for the rapid, selective and efficient removal of toxicants from complex natural systems.
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•We report the first use of COFs for selective removal of AAI from natural plants.•The iCOFs afford high selectivity and record high adsorption capacity for AAI.•The iCOFs exhibit excellent in vivo biocompatibility.
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•Acetobacter sp. and Gluconobacter sp. were the core microbes.•Fermentation were divided into three stages and completed at the end of second stage.•Butanoic acid and hexanoic acid ...were malodour substances of noni fruit juice.•Enzymes and metabolic pathways of microbiome were analyzed by metatranscriptomic.
Noni (Morinda citrifolia L.) has been recognized as an important herb for treating various physiological disorders worldwide. Fermented noni fruit juice, established as a novel food in European Union, is the most important noni product. However, the structure, functions and enzyme profiles of microbiome during fermentation remain unclear. The metatranscriptomic was used to comprehensively explore the active microbial community and key metabolic function. Acetobacter sp., Acetobacter aceti and Gluconobacter sp. were the major microorganisms and appeared in succession during fermentation. According to principal components analysis (PCA) of metabolism-related unigenes by KEGG database, the fermentation process was divided into three stages and almost completed at the end of the second stage. Furthermore, carbohydrate-active enzymes (CAZymes) and the expression of key enzymes in major metabolic pathways were analyzed systematically. Analysis by HS-SPME-GC–MS and odor active value (OAV) revealed that butanoic acid and hexanoic acid were the main volatile compounds for the unpleasant odor of fermented noni fruit juice. The microbiome in the fermentation process lacked key enzymes that degrade butanoic acid and hexanoic acid, which imparted rancid and sweat odor. This study provides theoretical basis for product improvement and new product development, thus promoting the development of noni food industry.
The synthesis and application of three-dimensional (3D) mesoporous covalent–organic frameworks (COFs) are still to be developed. Herein, two mesoporous 3D COFs with an stp topology were synthesized ...in a highly crystalline form with aniline as the modulator. The chemical composition of these COFs was confirmed by Fourier transform infrared (FT-IR) and 13C cross-polarization magic angle spinning nuclear magnetic resonance (NMR) spectroscopies. These 3D mesoporous COFs were highly crystalline and exhibited permanent porosity and good chemical stability in both aqueous and organic media. The space group and unit cell parameters of COF HFPTP-TAE were verified by powder X-ray diffraction (PXRD), small-angle X-ray scattering, and three-dimensional electron diffraction (3D ED). The appropriate pore size of the COF HFPTP-TAE facilitated the inclusion of enzyme lipase PS with a loading amount of 0.28 g g–1. The lipase⊂HFPTP-TAE (⊂ refers to “include in”) composite exhibited high catalytic activity, good thermal stability, and a wide range of solvent tolerance. Specifically, it could catalyze the alcoholysis of aspirin methyl ester (AME) with high catalytic efficiency. Oriented one-dimensional (1D) channel mesopores in HFPTP-TAE accommodated lipase, meanwhile preventing them from aggregation, while windows on the wall of the 1D channel favored molecular diffusion; thus, this COF-enzyme design outperformed its amorphous isomer, two-dimensional (2D) mesoporous COF, 3D mesoporous COF with limited crystallinity, and mesoporous silica as an enzyme host.
Our aim was to investigate the preparation of self-assembled garlic essential oil-amylose inclusion complexes (SGAs) using garlic essential oil (GEO) and corn starch (CS), and evaluated their release ...properties. SGAs were fabricated by pre-gelatinization coupling with high-speed shear at different GEO-CS mass ratios. When the mass ratio of GEO to pre-gelatinized corn starch was set at 15 % (SGA-15 %), with a fixed shear rate of 9000 rpm and a shear time of 30 min, the allicin content was 0.573 ± 0.023 mg/g. X-ray diffraction (XRD) results revealed a starch V-type crystalline structure in SGAs with peaks at 13.0°, 18.0°, and 20.0° (2θ). Fourier Transform Infrared (FTIR) spectra of SGAs displayed a shift in the characteristic peak of diallyl trisulfide from 987.51 cm
to 991.45 cm
. Scanning electron microscope (SEM) images revealed that SGAs exhibited lamellar structures covered with small granules. SGAs exhibited higher residual mass (approximately 12 %) than other samples. The resistant starch content of SGAs increased from 10.1 % to 18.4 % as GEO contents varied from 5 % to 15 %. In vitro digestion tests showed that about 53.21 % of allicin remained in SGA-15 % after 8 h. Therefore, this dual treatment can be a new method for fabricating controlled-release inclusion complexes of guest molecules.
The correlation of the corrosion behaviour and semiconductor characteristics of a passive film on Incoloy 825 in 5%~30% NH4Cl solutions was investigated using potentiodynamic polarization scan, ...Mott-Schottky curves，scanning electron microscope(SEM) and the point defect model(PDM).The results indicate that Incoloy 825 is self-passivated and has a pitting tendency in all four concentrations of NH4Cl solution at 50°C. With the increasing NH4Cl solution concentration, the quantities of chloride ions absorbed in passive films on Incoloy 825 increase, which can increase the quantities of cation vacancies for the Mott-Schottky reaction, so the quantities of point defects in the passive film on Incoloy 825 increase and the anodic dissolution and pitting of Incoloy 825 becomes easier. Therefore, the passive current density of Incoloy 825 increases, and the pitting potential of Incoloy 825 shifts negatively with the increasing concentration of ammonium chloride.
A detailed kinetic model for a continuous catalytic reforming (CCR) process was developed. The model included 447 naphtha molecules (C1–C12) that underwent 1469 reactions. Paraffin and naphthenic ...isomers up to C9 components were fully depicted, whereas aromatic isomers were fully described up to C10. Coking kinetics and the corresponding deactivation of the catalyst were integrated into the model. The steady state kinetic parameters were tuned using pilot plant data for a widely used industrial catalyst. To enable the use of commercial plant data, the energy balance and catalyst moving mechanism of typical CCR reactors were also formulated. The model was then used to simulate an industrial unit loaded with the same catalyst after deactivation calibration by adjusting a few deactivation parameters. The results showed that calculated PONA fractions, individual aromatic species, and the temperature drops of each reactor were in good accord with industrial data.
Enzyme immobilization is essential to the commercial viability of various critical large‐scale biocatalytic processes. However, challenges remain for the immobilization systems, such as difficulties ...in loading large enzymes, enzyme leaching, and limitations for large‐scale fabrication. Herein, we describe a green and scalable strategy to prepare high‐performance biocatalysts through in situ assembly of enzymes with covalent organic frameworks (COFs) under ambient conditions (aqueous solution and room temperature). The obtained biocatalysts have exceptional reusability and stability and serve as efficient biocatalysts for important industrial reactions that cannot be efficiently catalyzed by free enzymes or traditional enzyme immobilization systems. Notably, this versatile enzyme immobilization platform is applicable to various COFs and enzymes. The reactions in an aqueous solution occurred within a short timeframe (ca. 10–30 min) and could be scaled up readily (ca. 2.3 g per reaction).
In situ assembly of enzymes and covalent organic frameworks (COFs) enables the environmentally benign large‐scale fabrication of a new generation of high‐performance biocatalysts. This approach shows how limitations in enzyme immobilization can be overcome and it opens up a new avenue for the scalable fabrication of high‐performance biocatalysts to accelerate enzyme industrialization.
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