The authors herein present their personal series on the clinical evaluation of the analgesic efficacy of 2 opiodes, peridural morphine hydrochloride (0.5 mg/kg) administered to group A patients, and ...sublingual buprenorphine (0.4 mg) administered to group B patients in the management of post-operative pain in major abdominal surgery. The study was conducted on 44 patients, both male and female, classified as ASA II and III. Statistical analysis of cardiovascular parameters (BP and cardiac frequency), respiratory frequency and pain score showed an overlapping progression among the two groups. Spontaneous pain decreased significantly to zero at T6 (p < 0.001). However, the necessity to administer buprenorphine more frequently after the sixth hour and the consequent increase in side effects, lead us to believe that the method used for group A patients is more advantageous.
Investigation into the occurrence of glutamic acid in meat products Barbieri, G; Rivaldi, P. (Stazione Sperimentale per l'Industria delle Conserve Alimentari (SSICA), Parma (Italy)); Bernini, R ...
Industria Conserve (Italy),
(Oct-Dec 2002), Volume:
77, Issue:
4
Journal Article
Glutamic acid content was determined in some meat products in order to assess the amount of this substance purposely added; to this end, two different analytical methods were used, viz. an enzymatic ...method and the ion chromatographic technique (IC). The difference between products with or without added glutamic acid is statistically significant (up to 300 mg/kg product). In mortadella sausages and in cooked ham, in products with no added glutamate, a percentage of this substance lower than 0.06% on dry matter is found, whereas for frankfurters this percentage increases up to 0.075%. Roosted meat is the product with the lowest natural glutamate content (0.04%)
E' stato determinato il contenuto di acido glutammico in alcuni prodotti a base di carne, al fine di poter valutare la quantita' aggiunta intenzionalmente; sono stati impiegati a tale scopo due diversi metodi di analisi, un metodo enzimatico e la tecnica della cromatografia ionica (IC). La differenza tra prodotti additivati e no e' statisticamente significativa (fino a 300 mg per kg di prodotto). Nella mortadella e nel prosciutto cotto non additivati si riscontra una percentuale di glutammato sul secco inferiore allo 0,06%, mentre per i wurstel questa percentuale sale allo 0,075%. L'arrosto e' il prodotto con il contenuto piu' basso di glutammato naturale (0,04%)
We herein evaluated the analgesic effectiveness of low doses of buprenorphine with or without a primer and the practicality and usefulness of a constant flow disposable infusion set (Infusor Baxter 2 ...ml/h). Our series consists of 300 patients, seen between January 1992 and December 1993, randomly distributed in three groups of 100 patients each. All patients were informed before operation about the use of pain Visual Analogical Scale (VAS) and how the infusor works. At the end of the operation all patients had an infusor positioned with a 0.01 mg/kg supplied of buprenorphine diluted to 60 ml with Ringer's Lactate solution. In addition to this, group B patients received an i.v. primer of 0.003 mg/kg of buprenorphine diluted to 10 ml with saline solution and group C patients received an i.v. primer of 0.5 mg/kg of ketorolac diluted to 10 ml with saline solution. VAS, systolic arterial pressure, diastolic arterial pressure, heart rate, respiratory rate, request of analgesics and side effects were evaluated at one hour (T0), six hours (T1), 12 hours (T2), 24 hours (T3) and 36 hours (T4) after operation. Group B and C patients showed significant reduction in VAS compared to group A patients at T0 and T1 but the difference disappeared starting from T2. Group B and C patients showed blood pressure, heart rate and respiratory rate values significantly reduced compared to group A patients at T0 and T1. Such values however then stabilized. Side effects encountered were nausea and vomiting in different percentages in the three groups.
Buprenorphine was used as a preanesthetic drug for peridural anaesthesia. The aim of the study was therefore to evaluate the sedative/anxiolitic activity of this drug as compared to prometazine and ...diazepam. Two hundred male patients, divided into two groups (A and B), who were to undergo urological surgery under peripheral anesthesia, were included in the study: L2/L3 or L3/L4 epidural block. The group A patients were premedicated with buprenorphine; the group B patients received traditional anesthetic. The following factors were evaluated: the course of the AP and CF, the state of intraoperative sedation, the presence of side effects and data obtained, elaborated with a statistical test constructed to compare the percentages in two independent samples. There were no statistically significant variations of the AP and CF in the two groups. Additional sedatives were administered to 56.2% of the patients in group B and to only 27.1% in group A, premedicated with buprenorphine. Thirty seven percent of the group A patients and 10% of the group B patients presented preoperative nausea. The authors believe that the buprenorphine/atrophine association should be encouraged for pre-anesthesia when operations in peridural anesthesia are to be conducted, as it provides a state of constant sedation, avoiding rather ineffective pharmacological mixtures.
The euploid-diploid cell line CE, issuing spontaneously from a normal human bone marrow culture, displays B-cell differentiation and activation markers and is positive (> or = 90% of cells) for ...Epstein-Barr nuclear antigens. CE suspensions were inoculated with serum from a patient chronically infected with hepatitis C virus (HCV). After inoculation, the cells were regularly subcultured with a split ratio of 1:2 every 4-6 days. RNA extracted as late as 65 days after infection from the inoculated cells were positive by polymerase chain reaction for the 5' untranslated region of the HCV genome, and viral antigens were detected by immunofluorescence. Virus was also released from the infected cells into the medium. Intracellular HCV could be successfully passaged twice in CE cultures. On the basis of these findings, the CE cell line appears promising as a model for studying HCV replication and persistent infection in vitro.
