In April 2019, baobab (
L.) seedlings from Thailand, exhibiting galls on the roots, were intercepted during an import plant quarantine inspection at Chubu Centrair International Airport, Japan. ...Root-knot nematodes (RKNs) were extracted from the galled roots of baobab seedlings and identified by morphological, morphometrical and molecular methods as the guava root-knot nematode,
Yang & Eisenback. The morphology and morphometrics of the intercepted population were similar to those of the original and subsequent descriptions of
. The sequences of D2–D3 of 28S rRNA, mtDNA intergenic COII-16S rRNA and COI genes obtained in this study matched well (99–100% similarity) with each of the gene sequences of
deposited in GenBank. Phylogenetic analysis of these genes revealed that the intercepted population clustered with
and clearly differed from other RKN species. To the best of our knowledge, this is the first report of
from baobab.
The recurrence of chromosomal abnormalities in a specific subtype of cancer strongly suggests that dysregulated gene expression in the corresponding region has a critical role in disease ...pathogenesis. -7/7q-, defined as the entire loss of chromosome 7 and partial deletion of its long arm, is among the most frequently observed chromosomal aberrations in myeloid-lineage hematopoietic malignancies such as myelodysplastic syndrome and acute myeloid leukemia, particularly in patients treated with cytotoxic agents and/or irradiation. Tremendous efforts have been made to clarify the molecular mechanisms underlying the disease development, and several possible candidate genes have been cloned. However, the study is still underway, and the entire nature of this syndrome is not completely understood. In this review, we focus on the attempts to identify commonly deleted regions in patients with -7/7q-; isolate the candidate genes responsible for disease development, cooperative genes and the factors affecting disease prognosis; and determine effective and potent therapeutic approaches. We also refer to the possibility that the accumulation of multiple gene haploinsufficiency, rather than the loss of a single tumor suppressor gene, may contribute to the development of diseases with large chromosomal deletions such as -7/7q-.
Lysine-specific demethylase 1 (LSD1) regulates gene expression by affecting histone modifications and is a promising target for acute myeloid leukemia (AML) with specific genetic abnormalities. Novel ...LSD1 inhibitors, NCD25 and NCD38, inhibited growth of MLL-AF9 leukemia as well as erythroleukemia, megakaryoblastic leukemia and myelodysplastic syndromes (MDSs) overt leukemia cells in the concentration range that normal hematopoiesis was spared. NCD25 and NCD38 invoked the myeloid development programs, hindered the MDS and AML oncogenic programs, and commonly upregulated 62 genes in several leukemia cells. NCD38 elevated H3K27ac level on enhancers of these LSD1 signature genes and newly activated ~500 super-enhancers. Upregulated genes with super-enhancer activation in erythroleukemia cells were enriched in leukocyte differentiation. Eleven genes including GFI1 and ERG, but not CEBPA, were identified as the LSD1 signature with super-enhancer activation. Super-enhancers of these genes were activated prior to induction of the transcripts and myeloid differentiation. Depletion of GFI1 attenuated myeloid differentiation by NCD38. Finally, a single administration of NCD38 causes the in vivo eradication of primary MDS-related leukemia cells with a complex karyotype. Together, NCD38 derepresses super-enhancers of hematopoietic regulators that are silenced abnormally by LSD1, attenuates leukemogenic programs and consequently exerts anti-leukemic effect against MDS-related leukemia with adverse outcome.
Summary
Background
Vonoprazan, a potassium‐competitive acid blocker, is expected to improve the healing of endoscopic submucosal dissection (ESD)‐induced gastric ulcers compared with proton pump ...inhibitors (PPIs).
Aim
To compare the healing status of ESD‐induced gastric ulcers and the incidence of post‐ESD bleeding between subjects treated with vonoprazan for 5 weeks and those treated with PPIs for 8 weeks.
Methods
Patients in the vonoprazan group (n = 75) were prospectively enrolled, whereas patients in the PPI group (n = 150) were selected for a 2:1 matched historical control cohort according to baseline characteristics including gastric ulcer size immediately following ESD, age, sex and status of Helicobacter pylori infection. Two controls per case of vonoprazan‐treated group were matched with a margin of 20% in terms of ulcer size and a margin of 5 years in terms of their age.
Results
Although a higher number of completely healed ulcers was observed in the PPI group (95/150, 63.3%) than that in the vonoprazan group (14/75, 18.7%; P < 0.001), the ulcer size reduction rates, which were 96.0 ± 6.7% in the vonoprazan group and 94.7 ± 11.6% in the PPI group, were not significantly different (P = 0.373). The post‐ESD bleeding incidence in the vonoprazan group (1/75, 1.3%) was less than that in the PPI group (15/150, 10.0%; P = 0.01). The factors affecting post‐ESD bleeding incidence were the type of acid secretion inhibitor (P = 0.016) and use of an anti‐thrombotic agent (P = 0.014).
Conclusion
Vonoprazan significantly reduced post‐endoscopic submucosal dissection bleeding compared with PPIs.
Eu-doped GaN is a promising material for the active layer in red light emitting diodes. Although the output power of LEDs based on GaN:Eu has been increasing by a combination of structural and growth ...optimizations, there is still a significant limitation resulting from a poor light extraction efficiency, typical for high refractive index materials. Here we studied nanostructuring of the top of the optical active layer by nano-cubes for enhancement of the light extraction efficiency, and its effect on the optical emission characteristics. By etching nano-cubes into the active layer, we observed an increase in directional light output power of Eu
ions of up to 60%, as well as a grating effect. Simultaneously, the absorption of excitation light into the optical active layer was improved, leading to a 12.8 times increase of output power per available Eu
ion.
Developmental and aging changes in testicular factors related to steroidogenesis are unknown in dogs. Using reverse transcription quantitative real-time PCR, this study examined testicular mRNA ...levels of CYP11A1 (P450 cholesterol side-chain cleavage enzyme, P450scc), CYP17A1 (P450 17α-hydroxylase/C17–20 lyase, P450c17), HSD3B2 (3β-hydroxysteroid dehydrogenase, 3β-HSD), CYP19A (P450 aromatase, P450arom), STAR (steroidogenic acute regulatory protein, StAR), cyclooxygenase (COX) -1 and COX-2 in prepubertal (4–6 months of age), postpubertal (1 year of age), and aging (2–18 years of age) dogs. Testicular mRNA levels for P450scc, 3β-HSD, StAR, COX-1, and COX-2 did not change from prepubertal to postpubertal stages, whereas that for P450arom markedly and abruptly increased and that for P450c17 gradually decreased. In postpubertal and aging dogs, a negative correlation was found between aging and testicular P450arom mRNA levels. Based on the rapid testicular growth observed during puberty, these results suggested that total testis gene expression for steroidogenesis-related factors, in particular for P450arom, increases during puberty in dogs. In addition, the decline in P450arom gene expression during aging may affect the ability to synthesize steroids in canine testes.
We carried out 12CO(J = 1-0) observations of the Galactic gamma-ray supernova remnant (SNR) Kesteven 79 using the Nobeyama Radio Observatory 45 m radio telescope, which has an angular resolution of ...∼20 arcsec. We identified molecular and atomic gas interacting with Kesteven 79, whose radial velocity is ∼80 km s−1. The interacting molecular and atomic gases show good spatial correspondence with the X-ray and radio shells, which have an expanding motion with an expanding velocity of ∼4 km s−1. The molecular gas associated with the radio and X-ray peaks also exhibits a high intensity ratio of CO 3-2/1-0 > 0.8, suggesting a kinematic temperature of ∼24 K, owing to heating by the supernova shock. We determined the kinematic distance to the SNR to be ∼5.5 kpc and the radius of the SNR to be ∼8 pc. The average interstellar proton density inside of the SNR is ∼360 cm−3, of which atomic protons comprise only ∼10%. Assuming a hadronic origin for the gamma-ray emission, the total cosmic-ray proton energy above 1 GeV is estimated to be ∼5 × 1048 erg.
Linked ContentThis article is linked to Ban et al and Ugimoto and Kagawa papers. To view these articles visit https://doi.org/10.1111/apt.14202 and https://doi.org/10.1111/apt.13747.
We analyzed the 2.6 mm CO and 21 cm H i lines toward the Magellanic superbubble 30 Doradus C, in order to reveal the associated molecular and atomic gas. We uncovered five molecular clouds in a ...velocity range from 251 to 276 km s−1 toward the western shell. The non-thermal X-rays are clearly enhanced around the molecular clouds on a parsec scale, suggesting possible evidence for magnetic field amplification via shock-cloud interaction. The thermal X-rays are brighter in the eastern shell, where there are no dense molecular or atomic clouds, opposite to the western shell. The TeV γ-ray distribution may spatially match the total interstellar proton column density as well as the non-thermal X-rays. If the hadronic γ-ray is dominant, the total energy of the cosmic-ray protons is at least erg with the estimated mean interstellar proton density ∼60 cm−3. In addition, the γ-ray flux associated with the molecular cloud (e.g., MC3) could be detected and resolved by the Cherenkov Telescope Array (CTA). This should permit CTA to probe the diffusion of cosmic-rays into the associated dense ISM.
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