A voltage- and time-dependent conductance for sodium ions is responsible for the generation of impulses in most nerve and muscle cells. Changes in the sodium conductance are produced by the opening ...and closing of many discrete transmembrane channels. We present here the first report of electrical recordings from voltage-dependent sodium channels incorporated into planar lipid bilayers. In bilayers with many channels, batrachotoxin (BTX) induced a steady-state sodium current that was blocked by saxitoxin (STX) at nanomolar concentrations. All channels appeared in the bilayer with their STX blocking sites facing the side of vesicle addition, allowing us to define that as the extracellular side. Current fluctuations due to the opening and closing of single BTX-activated sodium channels were voltage-dependent (unit conductance, 30 pS in 0.5 M NaCl): the channels closed at large hyperpolarizing potentials. Slower fluctuations of the same amplitude, due to the blocking and unblocking of individual channels, were seen after addition of STX. Block of the sodium channels by STX was voltage-dependent, with hyperpolarizing potentials favouring block. The voltage-dependent gating, ionic selectivity and neurotoxin sensitivity suggest that these are the channels that normally underlie the sodium conductance change during the nerve impulse.
Measurements and simulations indicate that the particle-pair radial distribution function in isotropic turbulence is a power law in a range of length scales below the Kolmogorov scale for Stokes ...number St<<1. In this range, the exponent is proportional to St1St2 for unlike particles (1 and 2) in a bidispersion, hence St2 for a monodispersion. Here, this result is derived from a model of particle response to random advection. The analysis generalizes a geometrical interpretation of clustering to polydispersions and suggests an economical Monte Carlo simulation method.
We evaluated the effects of 2.5x10(-10) M or 5x10(-10) M concentrations of human pituitary prolactin (pPRL), human recombinant non-glycosylated (NG-PRL) and glycosylated (GL-PRL) prolactin on the ...proliferation of normal human lymphocytes with or without coactivation by interleukin-2 (IL-2). None of the PRL forms alone affected the lymphocyte proliferation in a serum-free medium, however, the stimulatory activity of IL-2 was significantly potentiated with all 3 PRL variants. Since the 5x10(-10) M concentrations of individual PRLs exerted the same effects, this result suggests that GL-PRL in primary lymphocyte culture is not a less mitogenic form, if sufficient amounts of IL-2 are available.
A solar cell using micro-antennas to convert radiation to alternating current and ultrahigh-speed diodes to rectify the AC can in principle provide extremely high conversion efficiencies. Currently ...investigated rectennas using metal/insulator/metal (MIM) diodes are limited in their RC response time and have poor impedance matching to the antenna. We have investigated a new rectifier, referred to as a geometric diode, which can overcome these limitations. The geometric diode consists of a conducting thin-film, such as graphene, patterned in a geometry that leads to diode behavior. We have experimentally demonstrated geometric diodes made from graphene and simulated their characteristics using the Drude model for charge transport. Here we compare the characteristics of rectennas using MIM diodes with those based on geometric diodes and show the improved performance of the latter.
This study evaluated the effects of a combined gestational and 10 day postnatal alcohol exposure (human three trimester equivalency) on the development of myelin and axons in rat optic nerve. Rats ...were exposed during gestation via liquid diet, then their artificially reared pups were further exposed for 10 postnatal days via an ethanol-containing diet fed by gastrostomy. Control animals from pair-fed dams were artificially reared for 10 days on pair-fed isocaloric diets. Anesthetized animals were perfused with fixative on gestational days (G) 15 and 20 and postnatal days (P) 5, 10, 15, 20, and 90, then optic nerve tissues prepared for electron microscopy. Optic nerve cross-sectional areas were generally less from G20 through P90 in ethanol exposed animals. Counts of the number of myelinated nerve fibers per unit area and of the numbers of fibers in different stages of myelin development revealed that alcohol exposure caused a delay in myelin acquisition at 10 and 15 days that was compensated for at 20 and 90 days. Myelin thickness as a function of axon diameter was decreased in the alcohol exposed animals from 10 through 90 days, indicating a permanent reduction in the relative thickness of myelin. These results show that alcohol exposure for all of gestation and 10 postnatal days in the rat (human three trimester equivalency) causes a permanent reduction in myelin thickness along with a delay in myelin acquisition in the optic nerve. Such alterations in developing and adult myelin could help to explain some of the neurological and visual dysfunctions associated with developmental alcohol exposures.
Different models to study the pathophysiology of cerebral ischemia and to evaluate therapeutic strategies exist. Described is the detailed procedure of a thromboembolic stroke model in the rat most ...closely resembling human embolic stroke and compare the model to other equivalent rodent models.
An evaluation of a new thromboembolic stroke model was performed on 35 male Wistar rats. After preparation of the carotid artery, a catheter was introduced into the external carotid artery. During injection of autologous, fibrin-rich emboli into the internal carotid artery the common carotid artery was temporarily occluded. Regional cerebral blood flow (rCBF) and lesion size were determined.
Twelve fibrin-rich blood clots of 1.5 mm in length were necessary in order reliably to occlude the origin of the middle cerebral artery. A stable decrease of rCBF and lesion size was confirmed by autoradiography, diffusion, and perfusion MRI, TTC-staining, biochemical imaging, and histology.
In this animal model, the situation of human cerebral ischemia is simulated closely. The model is suitable for investigations of the pathophysiology of stroke and facilitates studies on the effects of thrombolytic therapy.
The gene encoding the rat mitochondrial benzodiazepine receptor (MBR) was cloned and characterized. Hybridization of a previously cloned cDNA for MBR to genomic Southern blots indicated that the gene ...was probably present at one copy per haploid genome. Rapid amplification of cDNA ends with rat adrenal RNA was used to obtain 47 nt of additional sequence upstream from our previously cloned MBR cDNA proving to be a crucial step in cloning the first exon of this gene. The MBR gene is comprised of four exons spanning approx. 10 kb. The first intron, contained within a 8-kb stretch of this gene, is located within the 5'-untranslated sequence, whereas the remaining two introns are much shorter (641 and 854 bp) and interrupt the coding sequence. The third intron contains sequences homologous to rodent B1 repetitive elements and a novel sequence closely resembling part of a repetitive element belonging to the Alu family in humans. The transcription start point was mapped by S1 nuclease protection assays suggesting that the first exon is just 56 bp in length. The sequence upstream from this region contains three GC boxes but lacks other known consensus recognition sites for sequence-specific transcription factors.
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