Haemophilus parasuis and Glaesser's disease in pigs: a review Nedbalcova, K.(Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)) E-mail:nedbalcova@vri.cz; Satran, P.(Statni Veterinarni Sprava, Prague (Czech Republic). Odbor Ochrany Zdravi a Pohody Zvirat); Jaglic, Z.(Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)) ...
Veterinární medicína,
20/May , Volume:
51, Issue:
5
Journal Article
Peer reviewed
Haemophilus parasuis is a common epiphyte of the upper respiratory tract of pigs. The factors of H. parasis pathogenicity that enable some strains to be virulent and consequently cause a clinical ...disease have not been established yet. Fifteen serovars of H. parasuis have been described at present. Virulent strains can cause septicaemia without polyserositis or Glaesser's disease characterized by polyserositis, pericarditis, arthritis and meningitis. Clinical symptoms of this disease are highly variable. It can be treated with antibiotics. Commercial or autogenous vaccines can be used in the immunoprophylaxis of pre-parturient sows and their progeny after weaning.
Differences in the egg morphology of the invasive stored-product pests
Prostephanus truncatus and
Rhyzopertha dominica (Coleoptera: Bostrichidae) based on optical and scanning electron microscope ...(SEM) micrographs are presented. Diagnostic characteristics and changes in external egg appearance during development of the embryo are described. Significant differences between the eggs of these two species were found in their shape, size, surface structures, colour changes during development, and the thickness and number of eggshell layers.
Synthetic heptapeptide containing D-amino acid residues (Val-D-Leu-Pro-Phe-Phe-Val-D-Leu) was coupled to glyoxal-activated magnetic agarose particles via the free peptide amino group. The ...peptide-modified magnetic particles were used for the separation of pepsins. Porcine pepsin A and human pepsin A were adsorbed to the magnetic peptide-modified affinity carrier, while the rat pepsin C and human pepsin C did not interact with the immobilized ligand. Conditions of pepsin adsorption to peptide-modified magnetic particles, as well as elution buffers were optimized. Porcine pepsin A did not interact with the immobilized peptide in the presence of pepsin inhibitor pepstatin A, indicating that the enzyme binding site is involved in the studied interaction. The elaborated method represents a rapid and simple technique not only for the separation of pepsins but also, in combination with MS, for the enzyme detection and determination.
Microsporidia are a group of obligate intracellular protistan parasites that have recently gained recognition as opportunistic infections in persons with AIDS or in those who are otherwise ...immunocompromised. To date, eight genera have been identified as human pathogens, Encephalitozoon, Enterocytozoon, Nosema, Vittaforma, Pleistophora, Trachipleistophora, Brachiola, and the collective genus Microsporidium. A frequent causative agent of microsporidial diarrhea and systemic infection among AIDS patients is Encephalitozoon intestinalis. This parasite can also cause chronic diarrhea in immunocompetent persons. However, many epidemiologic aspects of this infection remain poorly understood as the diagnostic tools for this parasite are not amenable to widespread use. In an attempt to improve this situation, we have developed an ELISA test to detect human serum antibodies reactive with Encephalitozoon intestinalis.
The parthenogenetic psocid Lepinotus reticulatus Enderiein (Psocoptera: Trogiidae) is regarded as the common stored product pest in the world. This species has been identified as a new record in ...People's Republic (P.R) of China in this study. It was found in a sample of rice sweepings at grain storage in Lijiang, Yunnan, 2010. Decisive morphological characteristics of this new record species were described in detail and newly documented by scanning electron microscopy (SEM) micrographs. In the meantime, DNA barcode sequences of mtDNA COI of this geographical strain were tested and analyzed by neighbor-joining phylogenetic tree which showed that it was in the same sub-group with the U.S.A. stock. The origin of L. reticulatus and the necessity of continued comprehensive survey of stored product psocids were discussed.
The NA62 experiment at CERN collected a large sample of charged kaon decays in ight with a minimum bias trigger in 2007. Upper limits on the rate of the charged kaon decay into a muon and a heavy ...neutral lepton (HNL) obtained from this data are reported for a range of HNL masses. The NA62 experiment has collected further data in 2015 with a completely new and improved detector. New limits on heavy neutrinos from kaon decays into electron and HNL will be presented.
Affinity chromatography was used to study an interaction of aspartic proteinases with immobilized aromatic amino acids and their derivatives. The following ligands were used:
l-tyrosine, 3-iodo-
...l-tyrosine, 3,5-diiodo-
l-tyrosine,
l-phenylalanine,
p-iodo-
l-phenylalanine and
N-acetyl-
l-phenylalanine. With the exception of the last one, ligands were coupled directly to divinyl sulfone activated Sepharose 4B. For the preparation of immobilized
N-acetyl-
l-phenylalanine, divinyl sulfone activated Sepharose 4-B with linked ethylene diamine was used. Porcine pepsin was used for the evaluation of the capacity of the prepared affinity carriers. The capacity of the immobilized amino acid derivatives significantly increased in comparison with the non-derivatized amino acids. The prepared immobilized ligands were further used for the separation of human pepsinogens.
A
bstract
The NA62 experiment reports an investigation of the
K
+
→
π
+
ν
ν
¯
mode from a sample of
K
+
decays collected in 2017 at the CERN SPS. The experiment has achieved a single event ...sensitivity of (0
.
389
±
0
.
024)
×
10
−
10
, corresponding to 2.2 events assuming the Standard Model branching ratio of (8
.
4
±
1
.
0)
×
10
−
11
. Two signal candidates are observed with an expected background of 1.5 events. Combined with the result of a similar analysis conducted by NA62 on a smaller data set recorded in 2016, the collaboration now reports an upper limit of 1
.
78
×
10
−
10
for the
K
+
→
π
+
ν
ν
¯
branching ratio at 90% CL. This, together with the corresponding 68% CL measurement of (
0.48
−
0.48
+
0.72
)
×
10
−
10
, are currently the most precise results worldwide, and are able to constrain some New Physics models that predict large enhancements still allowed by previous measurements.
While mites are able to utilize numerous food sources, the suitability of the food strongly influences population growth. The different suitabilities of various stored agricultural products will thus ...affect the level of infestation. In this study, we compared field mite infestation rates in two stored cereals: wheat and barley. We analyzed mite abundance, frequency and species composition in samples of grain obtained from 79 selected Czech grain stores. Stored barley seemed to be more vulnerable to mite attack than wheat, as we consistently found more infested samples, more species and higher mean and median mite abundance per sample in barley as compared to wheat. The mean mite abundance per sample were 55 and 506 individuals for wheat and barley, respectively. In barley, 10% of samples exceeded allergen risk threshold (i.e., 1 000 individuals per kg of grain). Altogether, 25 species were identified from approximately 35 000 individuals. The most frequently identified species were the same in wheat and barley, that is, Tydeus interruptus Sig Thor, Acarus siro L., Tarsonemus granarius Lindquist, Lepidoglyphus destructor (Schrank) and Tyrophagus putrescentiae (Schrank). Based on principal components analysis, we found a closer association of T. interruptus, T. putrescentiae, L. destructor and Cheyletus eruditus (Schrank) with barley samples, corresponding to the high frequency and abundance values of these mites. The probable reasons for the higher infestation, especially mite abundance in barley, are discussed in relation to the higher proportion of crushed parts, which may release favorable nutrient sources and amplify the abundance values.
PDF
