•New adsorbents were prepared by combining chitosan and poly(1-vinylimidazole) using a facile technique.•High adsorption capacity was observed with the new adsorbent.•Synergistic effect was realized ...from chitosan and poly(1-vinylimidazole).•Interaction mechanism was also evaluated.
Chitosan and poly(1-vinylimidazole) are both potential adsorbents to remove Cr(VI). Here, we designed the preparation of new adsorbents by combining chitosan and poly(1-vinylimidazole) to get the synergistic effect for the removal of hexavalent chromium. Trimethoxysilyl group terminated poly(1-vinylimidazole)-modified-chitosan composite was successfully synthesized by one-step free radical polymerization based on the grafting backbone of chitosan and vinylimidazole. The resulting adsorbents were used for the removal of Cr(VI) ions from the aqueous solution. The modified chitosan composite was characterized by ATR, FTIR, BET isotherm studies, elemental analysis, TGA, DSC, FE-SEM, and EDX. ATR. FTIR results confirmed the presence of the imidazole group in modified chitosan. The adsorption results were described by the Langmuir isotherm model with a maximum adsorption capacity of 196.1 mg/g for modified chitosan, however, the chitosan yielded 151.5 mg/g. It has been observed that the adsorption of chromium fitted better with the pseudo-second-order kinetics. The modified chitosan composite exhibited greater adsorption capacity than chitosan for hexavalent chromium and has potential application for Cr(VI) removal from aqueous solution containing other common ions with regeneration ability. This novel approach of modifying chitosan with telomerized poly(1-vinyl imidazole) offers potential application in wastewater treatment of different industries releasing Cr (VI).
We demonstrate a versatile and facile method for fabrication of a new class of amphiphilic spherical nanoparticles having a nitrogen-enriched carbonised surface and precisely-controlled morphology. ...They are prepared by one-pot polymerization with β-lactoglobulin aggregates as a template with tunable size (70-750 nm) and mild heat-treatment to extend the π-conjugated structures.
The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process. The physico-chemical ...properties of the scaffolds were evaluated in order to assess its suitability for tissue engineering application. ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation. nHAp was synthesized from waste egg-shells by wet chemical method. The synthesized HAp was found crystalline and nanosize (~45 nm) by XRD and TEM analysis respectively. Ca to P ratio of nHAp is 1.51 as observed by EDX confirmed the suitability of the scaffold for biomedical application. The crosslinked ADA increases thermal stability of scaffolds. Water uptake and degradation ability significantly reduced with the increase of nHAp in the scaffold due to the higher stiffness contributed by nHAp. SEM analysis revealed that the pore size and porosity of the scaffolds declines with the proliferation of nHAp in the scaffolds. XRD analysis of the scaffolds shows the increase of crystallinity in the composites due to incorporation of nHAp and ADA. Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible. These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.
Herein, we present a facile approach to fabricate alginate-di-aldehyde (ADA) cross-linked gelatin/nano-hydroxyapatite (nHAp) bioscaffolds by lyophilization process for soft tissue engineering. Nano HAp was synthesized from waste eggshell and alginate di aldehyde was prepared by oxidation of Na-alginate. Oxidized alginate crosslinked with gelatin has enhanced cell growth, proliferation, and migration. The incorporation of biopolymers and bioceramic nHAp support the custom design of pours structure and physiological properties of 3D hydrogel scaffold. Display omitted
A multifunctional drug carrier with dual targeting (magnetic and folate-receptor) and pH sensitive core-shell hybrid nanomaterial has been developed to carry an anticancer drug ...doxorubicin.Superparamagnetic iron oxide nanoparticles (IONPs) were used as core of the carrier and cross-linked folate conjugated chitosan (FA-CS) was acted as shell in which doxorubicin was physically entrapped. Transmission electron microscopy (TEM) analysis confirmed the average particle size of IONPs and FA-CS coated IONPs 8.2 and 15.4 nm respectively. Magnetic measurement indicated that both the IONPs and FA-CS coated IONPs were superparamagnetic at room temperature with a magnetization value 57.72 and 37.44 emu/g respectively. At pH 5.8 (malignant tissue) showed a burst release of 30.05% of the doxorubicin in the first 4 h followed by a sustained release of 88.26% of drug over 72 h. From these results it is expected that doxorubicin loaded nanoparticles can be a promising drug carrier for the treatment of solid tumors with the ability to reduce toxic side effects of drugs by selective targeting and sustained release.
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Electrospinning of a heterogeneous solution is difficult to continue because the required process parameters are different for multiple phases. In this study, nanofibrous mats were successfully ...prepared from a heterogeneous blend of solid cellulose nanocrystals (CNC) and hydroxyapatite nanoparticles (HAp) in a solution mixture of chitosan and gelatin using an electrospinning technique. HAp and CNC were used as filler materials in the nanofibrous mats. Gelatin and chitosan polymer chains in the mats were crosslinked using glutaraldehyde. The fiber diameter was noticed to decrease from around 86 to 43 nm with the increase of electrical conductivity of the spinning solution from 890 to 1166 μS cm−1 and after crosslinking a significant variation in fibers’ diameter was noticed. The elemental analysis data showed that around 85% of the HAp used in the spinning solution was passed through the nozzle and the rest of the portion remained settled in the spinning syringe. In the XRD study, the crystallinity of chitosan, HAp and CNC was not observed in the non-crosslinked and crosslinked mats. The TGA analysis showed that the crosslinked mat has no weight retention at 500 °C which is due to its complete amorphous nature. The mats showed single-phase transition temperatures in DSC analysis which proves that no segregation of materials was present in the electrospun fibers. FTIR analysis of the mats showed a new peak at 1205 cm−1 which suggests the Michael addition type reactions to be happened between chitosan and gelatin. Cytotoxicity analysis of the mats on the vero-cell line showed around 95% of cell viability. The prepared mats were applied as wound dressings on a mice model experiment and 50% faster healing of wounds on the mice was noticed for the non-crosslinked mats than the control one.
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Creative design: An approach to preparing mixed monolayers of thiolated single‐stranded DNA (ssDNA) and oligo(ethylene glycol)s (OEG‐AT) in a broad range of compositions as well as ssDNA/OEG‐AT ...patterns of any required shape (see top figure) has been shown. A combination of this approach with surface‐initiated enzymatic polymerization allows complex 3D DNA nanostructures to be sculpted with high spatial precision (bottom).
Electrospun nanofibrous membranes are of great interest for tissue engineering, active material delivery, and wound dressing. These nanofibers possess unique three-dimensional (3D) interconnected ...porous structures that result in a higher surface-area-to-volume ratio and porosity. This study was carried out to prepare nanofibrous membranes by electrospinning a blend of PVA/chitosan polymeric solution functionalized with different ratios of copper oxide. Chitosan-stabilized CuO nanoparticles (CH-CuO NPs) were biosynthesized successfully utilizing chitosan as the capping and reducing agent. XRD analysis confirmed the monoclinic structure of CH-CuO NPs. In addition, the electrospun nanofibrous membranes were UV-crosslinked for a definite time. The membranes containing CH-CuO NPs were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectroscopy, ultraviolet–visible (UV–vis) spectrophotometry, and dynamic light scattering (DLS). SEM results showed the nanosize of the fiber diameter in the range of 147–207 nm. The FTIR spectroscopy results indicated the successful incorporation of CH-CuO NPs into the PVA/chitosan nanofibrous membranes. DSC analysis proved the enhanced thermal stability of the nanofibrous membranes due to UV-crosslinking. Swelling and degradation tests were carried out to ensure membrane stability. Greater antimicrobial activity was observed in the nanoparticle-loaded membrane. An in vitro release study of Cu2+ ions from the membrane was carried out for 24 h. The cytotoxicity of CH-CuO NP-incorporated membranes was investigated to estimate the safe dose of nanoparticles. An in vivo test using the CH-CuO NP-loaded PVA/chitosan membrane was conducted on a mice model, in which wound healing occurred in approximately 12 days. These results confirmed that the biocompatible, nontoxic nanofibrous membranes are ideal for wound-dressing applications.
We used a combination of synchrotron-based X-ray photoelectron spectroscopy (XPS) and angle-resolved near-edge X-ray absorption fine structure (NEXAFS) spectroscopy to study the chemical integrity, ...purity, and possible internal alignment of single-strand (ss) adenine deoxynucleotide (poly(A)) DNA brushes. The brushes were synthesized by surface-initiated enzymatic polymerization (SIEP) on a 25-mer of adenine self-assembled monolayer (SAM) on gold (A25-SH), wherein the terminal 3′-OH of the A25-SH serve as the initiation sites for SIEP of poly(A). XPS and NEXAFS spectra of poly(A) brushes were found to be almost identical to those of A25-SH initiator, with no unambiguous traces of contamination. Apart from the well-defined chemical integrity and contamination-free character, the brushes were found to have a high degree of orientational order, with an upright orientation of individual strands, despite their large thickness up to ∼55 nm, that corresponds to a chain length of at least several hundred nucleotides for individual ssDNA molecules. The orientational order exhibited by these poly(A) DNA brushes, mediated presumably by base stacking, was found to be independent of the brush thickness as long as the packing density was high enough. The well-defined character and orientational ordering of the ssDNA brushes make them a potentially promising system for different applications.
Wound healing requires a substantial amount of moisture for faster recovery. Completely hydrophobic or hydrophilic biomaterials are not suitable to be applied for cell growth in wounded areas. The ...study aimed to prepare a nanofibrous scaffold from the blend of a solution of hydrophobic PLA and a solution of hydrophilic gelatine. The stability of the blend was achieved using a surfactant and an electrospun nanofibrous scaffold was made out of the solution. The optimum composition of gelatine and PLA to make a scaffold of uniform fibre diameter was achieved with the help of conductivity, viscosity and FESEM analysis. The optimum scaffold was characterised by TGA, DSC and XRD analysis. The water contact angle of the optimum sample was observed at 27°. The blended scaffold was found non-toxic to cells and showed a 30% faster healing of wounds in the rat model test compared to the healing rate of the PLA scaffold or the gelatine scaffold alone. The histological assay also supported the blend scaffold as an encouraging material for tissue regeneration.Wound healing requires a substantial amount of moisture for faster recovery. Completely hydrophobic or hydrophilic biomaterials are not suitable to be applied for cell growth in wounded areas. The study aimed to prepare a nanofibrous scaffold from the blend of a solution of hydrophobic PLA and a solution of hydrophilic gelatine. The stability of the blend was achieved using a surfactant and an electrospun nanofibrous scaffold was made out of the solution. The optimum composition of gelatine and PLA to make a scaffold of uniform fibre diameter was achieved with the help of conductivity, viscosity and FESEM analysis. The optimum scaffold was characterised by TGA, DSC and XRD analysis. The water contact angle of the optimum sample was observed at 27°. The blended scaffold was found non-toxic to cells and showed a 30% faster healing of wounds in the rat model test compared to the healing rate of the PLA scaffold or the gelatine scaffold alone. The histological assay also supported the blend scaffold as an encouraging material for tissue regeneration.
