Pasteurella multocida and Haemophilus parasuis pig isolates obtained in the Czech Republic were tested for their susceptibility against selected antimicrobial agents by broth microdilution method ...between 2008 and 2011. A low degree of resistance was observed for ampicillin, amoxicillin/clavulanic acid, ceftiofur, tulathromycin, tilmicosin, florfenicol and enrofloxacin in 20 (6.0%), 15 (4.5 %), 2 (0.6%), 8 (2.4%), 13 (3.9%), 5 (1.5%) and 5 (1.5%) P. multocida isolates as well as for tiamulin, gentamicin, tulathromycin, tilmicosin and ampicillin in 2 (2.4%), 2 (2.4%), 3 (3.6%), 3 (3.6%) and 6 (7.2%) H. parasuis isolates. In addition, moderate level of resistance to tiamulin was found in 60 (18.1%) P. multocida isolates and high level of resistance for tetracycline was detected in 107 (32.2 %) P. multocida isolates and in 23 (27.7 %) H. parasuis isolates. Differences between resistance rates of P. multocida and H. parasuis were significant (P less or equal 0.5) only for tiamulin. These data confirmed that antimicrobial resistance is not very widespread among current porcine P. multocida and H. parasuis isolates in the Czech Republic.
This study was focused on comparison of protectivity of whole-cell inactivated vaccine containing Actinobacillus pleuropneumoniae serotype 9 and a toxoid vaccine containing Apx I, Apx II, Apx III ...toxins and outer membrane protein (OMP). Twenty-one piglets from a commercial pleuropneumonia-free herd were used in the experiment. Piglets were randomly divided to three groups, seven animals each. Animals of the first group were vaccinated with the toxoid vaccine and those of the second group were treated with the whole-cell inactivated vaccine. The third group was an untreated control. Piglets aged 6 weeks were vaccinated for the first time and revaccinated at the age of 10 weeks. Two weeks following revaccination i.e. at the age of 12 weeks, piglets were inoculated endotracheally with a field strain A. pleuropneumoniae serotype 9, to yield an inoculum of 3.2 x 10**7 CFU/pig. Experimental infection resulted in the development of clinical pleuropneumonia in all animals. The survival rates were 100%, 71.4% and 14.3% in the first, second and third group, respectively. The clinical course of infection differed among the groups. In animals vaccinated with the toxoid vaccine clinical state turned to normal from day 3 following infection, in those vaccinated with whole-cell vaccine it was from day 5, and in untreated animals the symptoms persisted over the whole experimental period. The experiment was terminated 7 days following infection by sacrificing the piglets that survived the infection, and the degree of pulmonary lesions was determined at necropsy. Pulmonary scores were 6.3, 17.8 and 29.0 in animals vaccinated with the toxoid vaccine, whole-cell vaccine and non-vaccinated ones, respectively. Microbiological examinations confirmed the presence of A. pleuropneumoniae following the infection in tonsils.
Limited data regarding the susceptibility of to antimicrobials has been published during recent years. Accordingly, the aim of the present study was to investigate the distribution of MICs for the ...isolates of from diseased pigs in the Czech Republic between 2007 and 2009.
Haemophilus parasuis and Glaesser's disease in pigs: a review Nedbalcova, K.(Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)) E-mail:nedbalcova@vri.cz; Satran, P.(Statni Veterinarni Sprava, Prague (Czech Republic). Odbor Ochrany Zdravi a Pohody Zvirat); Jaglic, Z.(Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)) ...
Veterinární medicína,
20/May , Volume:
51, Issue:
5
Journal Article
Peer reviewed
Haemophilus parasuis is a common epiphyte of the upper respiratory tract of pigs. The factors of H. parasis pathogenicity that enable some strains to be virulent and consequently cause a clinical ...disease have not been established yet. Fifteen serovars of H. parasuis have been described at present. Virulent strains can cause septicaemia without polyserositis or Glaesser's disease characterized by polyserositis, pericarditis, arthritis and meningitis. Clinical symptoms of this disease are highly variable. It can be treated with antibiotics. Commercial or autogenous vaccines can be used in the immunoprophylaxis of pre-parturient sows and their progeny after weaning.
Differences in the egg morphology of the invasive stored-product pests
Prostephanus truncatus and
Rhyzopertha dominica (Coleoptera: Bostrichidae) based on optical and scanning electron microscope ...(SEM) micrographs are presented. Diagnostic characteristics and changes in external egg appearance during development of the embryo are described. Significant differences between the eggs of these two species were found in their shape, size, surface structures, colour changes during development, and the thickness and number of eggshell layers.
Synthetic heptapeptide containing D-amino acid residues (Val-D-Leu-Pro-Phe-Phe-Val-D-Leu) was coupled to glyoxal-activated magnetic agarose particles via the free peptide amino group. The ...peptide-modified magnetic particles were used for the separation of pepsins. Porcine pepsin A and human pepsin A were adsorbed to the magnetic peptide-modified affinity carrier, while the rat pepsin C and human pepsin C did not interact with the immobilized ligand. Conditions of pepsin adsorption to peptide-modified magnetic particles, as well as elution buffers were optimized. Porcine pepsin A did not interact with the immobilized peptide in the presence of pepsin inhibitor pepstatin A, indicating that the enzyme binding site is involved in the studied interaction. The elaborated method represents a rapid and simple technique not only for the separation of pepsins but also, in combination with MS, for the enzyme detection and determination.
Microsporidia are a group of obligate intracellular protistan parasites that have recently gained recognition as opportunistic infections in persons with AIDS or in those who are otherwise ...immunocompromised. To date, eight genera have been identified as human pathogens, Encephalitozoon, Enterocytozoon, Nosema, Vittaforma, Pleistophora, Trachipleistophora, Brachiola, and the collective genus Microsporidium. A frequent causative agent of microsporidial diarrhea and systemic infection among AIDS patients is Encephalitozoon intestinalis. This parasite can also cause chronic diarrhea in immunocompetent persons. However, many epidemiologic aspects of this infection remain poorly understood as the diagnostic tools for this parasite are not amenable to widespread use. In an attempt to improve this situation, we have developed an ELISA test to detect human serum antibodies reactive with Encephalitozoon intestinalis.
The parthenogenetic psocid Lepinotus reticulatus Enderiein (Psocoptera: Trogiidae) is regarded as the common stored product pest in the world. This species has been identified as a new record in ...People's Republic (P.R) of China in this study. It was found in a sample of rice sweepings at grain storage in Lijiang, Yunnan, 2010. Decisive morphological characteristics of this new record species were described in detail and newly documented by scanning electron microscopy (SEM) micrographs. In the meantime, DNA barcode sequences of mtDNA COI of this geographical strain were tested and analyzed by neighbor-joining phylogenetic tree which showed that it was in the same sub-group with the U.S.A. stock. The origin of L. reticulatus and the necessity of continued comprehensive survey of stored product psocids were discussed.
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