Different sanitization methods were evaluated as alternatives to formaldehyde fumigation for the reduction of eggshell and yolk sac microbiological counts, improvement of eggshell quality, incubation ...parameters, and day-old chick quality. A total of 10,080 hatching eggs were collected from a 70-wk-old commercial broiler breeder flock and distributed in a completely randomized block design with seven treatments: fumigation with paraformaldehyde (5.03 g/m3/30 min), fumigation with ozone (5-15 ppm/30 min), ultraviolet light-C irradiation (8.09 mW/cm2; 120 s; UV-C), hydrogen peroxide spraying (3%; 0.69 mL/egg), peracetic acid spraying (0.3%; 0.69 mL/egg; PAA), water spraying (0.69 mL/egg; water control), and without disinfection (dry control-DC). Spraying eggs with PAA and UV-C significantly reduced aerobic bacteria plate counts compared to the DC group. In addition, eggs disinfected with PAA had lower Enterobacteriaceae counts than the DC and water control groups. Eggshell quality, incubation parameters, and microbiological counts for yolk sac did not differ (P > 0.05) among treatments. This study demonstrated the potential for the application of PAA and UV-C for eggshell disinfection instead of formaldehyde; however, an electronic microscopic evaluation of the eggshell is necessary to determine if these methods cause any damage to the cuticle.
Biodiversity is declining on a global scale. Especially tropical ecosystems, containing most of the planetary biodiversity, are at risk. Agricultural monocrop systems contribute to this decline as ...they replace original habitats and depend on extensive use of synthetic pesticides that impact ecosystems. In this review we use large-scale banana production for export purposes in Costa Rica as an example for pesticide impacts, as it is in production for over a century and uses pesticides extensively for more than fifty years. We summarise the research on pesticide exposure, effects and risks for aquatic and terrestrial environment, as well as for human health. We show that exposure to pesticides is high and relatively well-studied for aquatic systems and humans, but hardly any data are available for the terrestrial compartment including adjacent non target ecosystems such as rainforest fragments. Ecological effects are demonstrated on an organismic level for various aquatic species and processes but are not available at the population and community level. For human health studies exposure evaluation is crucial and recognised effects include various types of cancer and neurobiological dysfunctions particularly in children. With the many synthetic pesticides involved in banana production, the focus on insecticides, revealing highest aquatic risks, and partly herbicides should be extended to fungicides, which are applied aerially over larger areas. The risk assessment and regulation of pesticides so far relies on temperate models and test species and is therefore likely underestimating the risk of pesticide use in tropical ecosystems, with crops such as banana. We highlight further research approaches to improve risk assessment and, in parallel, urge to follow other strategies to reduce pesticides use and especially hazardous substances.
Young refugees are a high-risk group for mental disorders, particularly for posttraumatic stress disorder (PTSD). Individuals with PTSD suffer from an altered time perspective with a focus on ...negative experiences in the past and a disregard for positive life events and the future. This study investigates time perspectives and psychological distress in 30 adolescent refugees from Syria and Afghanistan in Germany. Time perspective was assessed using the Zimbardo Time Perspective Inventory. The refugees exhibited a distorted time perspective that is common for individuals with PTSD: a high orientation toward the negative past and a low orientation toward the positive past and future. A high orientation toward the negative past was associated with high levels of general psychological distress, PTSD symptoms, and depression. A low orientation toward the future was related to high levels of anxiety. Taking time perspective and corresponding beliefs into consideration may be advantageous when treating refugees with trauma-related disorders.
A subgroup has emerged within the obese that do not display the typical metabolic disorders associated with obesity and are hypothesized to have lower risk of complications. The purpose of this ...review was to analyze the literature which has examined the burden of cardiovascular disease (CVD) and all-cause mortality in the metabolically healthy obese (MHO) population.
Pubmed, Cochrane Library, and Web of Science were searched from their inception until December 2012. Studies were included which clearly defined the MHO group (using either insulin sensitivity and/or components of metabolic syndrome AND obesity) and its association with either all cause mortality, CVD mortality, incident CVD, and/or subclinical CVD.
A total of 20 studies were identified; 15 cohort and 5 cross-sectional. Eight studies used the NCEP Adult Treatment Panel III definition of metabolic syndrome to define "metabolically healthy", while another nine used insulin resistance. Seven studies assessed all-cause mortality, seven assessed CVD mortality, and nine assessed incident CVD. MHO was found to be significantly associated with all-cause mortality in two studies (30%), CVD mortality in one study (14%), and incident CVD in three studies (33%). Of the six studies which examined subclinical disease, four (67%) showed significantly higher mean common carotid artery intima media thickness (CCA-IMT), coronary artery calcium (CAC), or other subclinical CVD markers in the MHO as compared to their MHNW counterparts.
MHO is an important, emerging phenotype with a CVD risk between healthy, normal weight and unhealthy, obese individuals. Successful work towards a universally accepted definition of MHO would improve (and simplify) future studies and aid inter-study comparisons. Usefulness of a definition inclusive of insulin sensitivity and stricter criteria for metabolic syndrome components as well as the potential addition of markers of fatty liver and inflammation should be explored. Clinicians should be hesitant to reassure patients that the metabolically benign phenotype is safe, as increased risk cardiovascular disease and death have been shown.
Health care- and device-associated central nervous system (CNS) infections have a distinct epidemiology, pathophysiology, and microbiology that require a unique diagnostic approach. Most clinical ...signs, symptoms, and tests used to diagnose community-acquired CNS infections are insensitive and nonspecific in neurosurgical patients due to postsurgical changes, invasive devices, prior antimicrobial exposure, and underlying CNS disease. The lack of a standardized definition of infection or diagnostic pathway has added to this challenge. In this review, we summarize the epidemiology, microbiology, and clinical presentation of these infections, discuss the issues with existing microbiologic tests, and give an overview of the current diagnostic approach.
Context.
HD 209458 b is an exoplanet with an upper atmosphere undergoing blow-off escape that has mainly been studied using measurements of the Ly
α
absorption. Recently, high-resolution measurements ...of absorption in the He
I
triplet line at 10 830 Å of several exoplanets (including HD 209458 b) have been reported, creating a new opportunity to probe escaping atmospheres.
Aims.
We aim to better understand the atmospheric regions of HD 209458 b from where the escape originates.
Methods.
We developed a 1D hydrodynamic model with spherical symmetry for the HD 209458 b thermosphere coupled with a non-local thermodynamic model for the population of the He
I
triplet state. In addition, we performed high-resolution radiative transfer calculations of synthetic spectra for the helium triplet lines and compared them with the measured absorption spectrum in order to retrieve information about the atmospheric parameters.
Results.
We find that the measured spectrum constrains the H/H
+
transition altitude occurring in the range of 1.2
R
P
–1.9
R
P
. Hydrogen is almost fully ionised at altitudes above 2.9
R
P
. We also find that the X-ray and extreme ultraviolet absorption takes place at effective radii from 1.16 to 1.30
R
P
, and that the He
I
triplet peak density occurs at altitudes from 1.04 to 1.60
R
P
. Additionally, the averaged mean molecular weight is confined to the 0.61–0.73 g mole
−1
interval, and the thermospheric H/He ratio should be larger than 90/10, and most likely approximately 98/2. We also provide a one-to-one relationship between mass-loss rate and temperature. Based on the energy-limited escape approach and assuming heating efficiencies of 0.1–0.2, we find a mass-loss rate in the range of (0.42–1.00) ×10
11
g s
−1
and a corresponding temperature range of 7125–8125 K.
Conclusions.
The analysis of the measured He
I
triplet absorption spectrum significantly constrains the thermospheric structure of HD 209458 b and advances our knowledge of its escaping atmosphere.
Aims. The Rosetta space probe accompanied comet 67P/Churyumov-Gerasimenko for more than two years, obtaining an unprecedented amount of unique data of the comet nucleus and inner coma. This has ...enabled us to study its activity almost continuously from 4 au inbound to 3.6 au outbound, including the perihelion passage at 1.24 au. This work focuses identifying the source regions of faint jets and outbursts and on studying the spectrophotometric properties of some outbursts. We use observations acquired with the OSIRIS/NAC camera during July–October 2015, that is, close to perihelion. Methods. We analyzed more than 2000 images from NAC color sequences acquired with 7–11 filters covering the 250–1000 nm wavelength range. The OSIRIS images were processed with the OSIRIS standard pipeline up to level 3, that is, converted in radiance factor, then corrected for the illumination conditions. For each color sequence, color cubes were produced by stacking registered and illumination-corrected images. Results. More than 200 jets of different intensities were identified directly on the nucleus. Some of the more intense outbursts appear spectrally bluer than the comet dark terrain in the visible-to-near-infrared region. We attribute this spectral behavior to icy grains mixed with the ejected dust. Some of the jets have an extremely short lifetime. They appear on the cometary surface during the color sequence observations, and vanish in less than some few minutes after reaching their peak. We also report a resolved dust plume observed in May 2016 at a resolution of 55 cm pixel−1, which allowed us to estimate an optical depth of ~0.65 and an ejected mass of ~2200 kg, assuming a grain bulk density of ~800 kg m−3. We present the results on the location, duration, and colors of active sources on the nucleus of 67P from the medium-resolution (i.e., 6–10 m pixel−1) images acquired close to perihelion passage. The observed jets are mainly located close to boundaries between different morphological regions. Some of these active areas were observed and investigated at higher resolution (up to a few decimeter per pixel) during the last months of operations of the Rosetta mission. Conclusions. These observations allow us to investigate the link between morphology, composition, and activity of cometary nuclei. Jets depart not only from cliffs, but also from smooth and dust-covered areas, from fractures, pits, or cavities that cast shadows and favor the recondensation of volatiles. This study shows that faint jets or outbursts continuously contribute to the cometary activity close to perihelion passage, and that these events are triggered byillumination conditions. Faint jets or outbursts are not associated with a particular terrain type or morphology.
Current management for diabetes has stimulated the development of versatile 3D-based hydrogels as in vitro platforms for insulin release and as support for the encapsulation of pancreatic cells and ...islets of Langerhans. This work aimed to create agarose/fucoidan hydrogels to encapsulate pancreatic cells as a potential biomaterial for diabetes therapeutics. The hydrogels were produced by combining fucoidan (Fu) and agarose (Aga), marine polysaccharides derived from the cell wall of brown and red seaweeds, respectively, and a thermal gelation process. The agarose/fucoidan (AgaFu) blended hydrogels were obtained by dissolving Aga in 3 or 5 wt % Fu aqueous solutions to obtain different proportions (4:10; 5:10, and 7:10 wt). The rheological tests on hydrogels revealed a non-Newtonian and viscoelastic behavior, while the characterization confirmed the presence of the two polymers in the structure of the hydrogels. In addition, the mechanical behavior showed that increasing Aga concentrations resulted in hydrogels with higher Young's modulus. Further, the ability of the developed materials to sustain the viability of human pancreatic cells was assessed by encapsulation of the 1.1B4HP cell line for up to 7 days. The biological assessment of the hydrogels revealed that cultured pancreatic beta cells tended to self-organize and form pseudo-islets during the period studied.
The manipulation of the photoperiod by the use of lighting programmes is a useful tool, inexpensive and can help to reduce the negative effect on feed intake due to the stress caused by hot ...environments. Lighting programmes for broiler chickens are used to regulate feed intake, providing access to food and water, especially during the cooler hours of the day. The change in the length of the photoperiod can be an alternative way to improve the welfare, immune response and, consequently, the performance of birds that are subject to heat stress. The purpose of this paper was to review relevant research on the use of lighting programmes for broilers.
PDF
