In this paper, we first systematically sorted out the characteristics of pop singing and musical theater singing and explored the generation of emotion and melody application of pop singing in ...musical theater singing. Then, the gray
(1,1) model is analyzed, and a gray data mining model based on data analysis is constructed to predict the application of pop singing in combination with the data mining model under data analysis. Finally, the prediction analysis was conducted to use characterization, timbre and style in pop singing, respectively. The results showed that the score of character building was 90, and the errors between the predicted and actual values of timbre and style were between ±0.1 and ±1, respectively, which were within an acceptable range.
The root of Rheum officinale BAILL as a traditional Chinese medicine, which main function is removing heat from the blood, promoting blood circulation and clearing toxins away. Rhein ...(4,5-dihydroxyanthraquinone-2-carboxylic acid) is one of the most important active components in the root of Rheum officinale BAILL, which could inhibit the proliferation of tumor cells. However, the study on the mechanism of anti-cell migration capacity of Rhein on ovarian cancer is not yet clear. Here, we demonstrated that Rhein had dose-dependent effects of ovarian tumors on drugs and could inhibit the proliferations and migration of two typical ovarian cancer cell lines, A2780 and OV2008. Furthermore, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays showed that the survival rate of ovarian cancer cells was significantly decreased when treated with Rhein. Rhein inhibited the proliferation of ovarian cancer cells in dose-dependent manner. Moreover, the wound healing assay and transwell assay indicated that the cell migratory potential and expression of matrix metalloproteinases were markedly inhibited by Rhein. Our findings suggested that Rhein could be a potential candidate to be developed as a drug for the prevention of ovarian cancer cell migration.
Cloud computing is becoming an increasingly popular platform for the execution of scientific applications such as scientific workflows. In contrast to grids and other traditional high-performance ...computing systems, clouds provide a customizable infrastructure where scientific workflows can provision desired resources ahead of the execution and set up a required software environment on virtual machines (VMs). Nevertheless, various challenges, especially its quality-of-service prediction and optimal scheduling, are yet to be addressed. Existing studies mainly consider workflow tasks to be executed with VMs having time-invariant, stochastic, or bounded performance and focus on minimizing workflow execution time or execution cost while meeting the quality-of-service requirements. This work considers time-varying performance and aims at minimizing the execution cost of workflow deployed on Infrastructure-as-a-Service clouds while satisfying Service-Level-Agreements with users. We employ time-series-based approaches to capture dynamic performance fluctuations, feed a genetic algorithm with predicted performance of VMs, and generate schedules at run-time. A case study based on real-world third-party IaaS clouds and some well-known scientific workflows show that our proposed approach outperforms traditional approaches, especially those considering time-invariant or bounded performance only.
Cryptotanshinone, a natural compound isolated from the roots of Salvia miltiorrhiza Bge. (Danshen), is a commonly used traditional Chinese medicine to treat high blood pressure in some countries. It ...has been shown that Cryptotanshinone induces cancer cells apoptosis and impairs cell migration and invasion. However, the antiproliferation and chemosensitization effects of Cryptotanshinone on ovarian cancer and the underlying mechanism are not fully elucidated.
In this study, we evaluated the inhibitory effect of Cryptotanshinone on ovarian cancer cells and explored the underlying molecular mechanism. Additionally, the chemosensitization potential of Cryptotanshinone was evaluated in combination with cisplatin.
MTT assay was used for cell viability assessment of ovarian cancer A2780 cells treated with Cryptotanshinone and/ or cisplatin. Flow cytometry was used for apoptosis analysis. Wound healing and transwell assays were used for migratory and invasive potential assessment of Cryptotanshinone-treated ovarian cancer cells. Western blot was used to investigate proteins involved in the mechanisms for metastasis and apoptosis. γH2AX immunocytochemistry was used to detect DNA damage in A2780 cells exposed to Cryptotanshinone and/or cisplatin.
Cryptotanshinone significantly induced ovarian cancer A2780 cells apoptosis by activating caspase cascade. Additionally, wound healing and transwell assays revealed that Cryptotanshinone could suppress migration and invasion of ovarian cancer cells and dramatically inhibited MMP-2 and MMP-9 expression. Furthermore, Cryptotanshinone could sensitize A2780 cells to cisplatin treatment in a dose-dependent manner.
Our data confirmed the anti-tumor effect of Cryptotanshinone on ovarian cancer cells and provided new findings that Cryptotanshinone could sensitize ovarian cancer cells to chemotherapy.
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Academic journals and discipline construction in colleges are two major drivers of scientific progress. There is a complex interactive mechanism between the two drivers. The synergy between the two ...is worthy of in-depth research. The research aims include better developing academic journals with discipline advantages and back-feeding discipline construction with academic journals. The symbiotic theory provides a new research perspective for the cooperation mechanism between college academic journals and discipline construction. Drawing on the symbiotic theory, this paper explores the root causes and interactive logic of the collaboration between college academic journals and discipline construction, from the angles of the symbiotic unit, symbiotic environment, and symbiotic model. The results show that the symbiosis between academic journals and discipline construction in Chinese colleges mainly relies on two essential parameters, namely, academic talents and research results; although colleges provide a positive environment, the symbiosis between the two factors mostly takes place on unofficial information platforms and cooperation mechanisms, for the relevant policies are relatively new and mostly discipline-oriented; the symbiosis between college academic journals and discipline construction is mutualistic but remains in the intermittent stage. Therefore, it is important to find a more effective symbiotic path for college academic journals and discipline construction, following their symbiotic law.
Epithelial ovarian cancer (EOC) is one of the most prevalent malignancies affecting females worldwide; however, its etiology mechanism remains unclear. In various malignancies, miR-145-5p is a widely ...accepted and versatile miRNA. Therefore, our research focused on exploring the activity and etiology of miR-145-5p in the modulation of metastasis, migration, and proliferation of EOC cells. The direct reactions between the 3'UTRs of SMAD4 mRNA and miR-145-5p were verified using dual luciferase reporter test. SKOV-3 cells were subsequently transfected using miR-145-5p mimics. Cell migration, death, and proliferation were evaluated using MTT, flow cytometry, and Transwell test. In addition, SMAD4 transcription and translation were evaluated using qRT-PCR and Western blot.
We found that miR-145-5p expression was repressed prevalently in EOC tissues, apart from SMAD4 upregulation. Excessive miR-145-5p expression remarkably reinforced EOC cell death and repressed EOC cell proliferation. Furthermore, upregulated miR-145-5p expression noticeably repressed migration via MMP-2 and MMP-9 downregulation. Moreover, SMAD4 was downregulated via miR-145-5p transfection. The dual luciferase test revealed that miR-145-5p directly targeted SMAD4.
Our research suggests that miR-145-5p serves as a malignancy repressor and exerts an essential impact on inhibiting malignancy generation and reinforcing EOC death via targeting SMAD4. MiR-145-5p application could serve as a promising strategy to treat EOC.
The COVID-19 is an infectious disease caused by SARS-CoV-2 infection. A large number of clinical studies found high-level expression of pro-inflammatory cytokines in patients infected with ...SARS-CoV-2, which fuels the rapid development of the disease. However, the specific molecular mechanism is still unclear. In this study, we found that SARS-CoV-2 Nsp5 can induce the expression of cytokines IL-1β, IL-6, TNF-α, and IL-2 in Calu-3 and THP1 cells. Further research found that Nsp5 enhances cytokine expression through activating the NF-κB signaling pathway. Subsequently, we investigated the upstream effectors of the NF-κB signal pathway on Nsp5 overexpression and discovered that Nsp5 increases the protein level of MAVS. Moreover, Nsp5 can promote the SUMOylation of MAVS to increase its stability and lead to increasing levels of MAVS protein, finally triggering activation of NF-κB signaling. The knockdown of MAVS and the inhibitor of SUMOylation treatment can attenuate Nsp5-mediated NF-κB activation and cytokine induction. We identified a novel role of SARS-CoV-2 Nsp5 to enhance cytokine production by activating the NF-κB signaling pathway.
Human Endogenous Retrovirus type K (HERV-K) is the only HERV known to be insertionally polymorphic; not all individuals have a retrovirus at a specific genomic location. It is possible that HERV-Ks ...contribute to human disease because people differ in both number and genomic location of these retroviruses. Indeed viral transcripts, proteins, and antibody against HERV-K are detected in cancers, auto-immune, and neurodegenerative diseases. However, attempts to link a polymorphic HERV-K with any disease have been frustrated in part because population prevalence of HERV-K provirus at each polymorphic site is lacking and it is challenging to identify closely related elements such as HERV-K from short read sequence data. We present an integrated and computationally robust approach that uses whole genome short read data to determine the occupation status at all sites reported to contain a HERV-K provirus. Our method estimates the proportion of fixed length genomic sequence (k-mers) from whole genome sequence data matching a reference set of k-mers unique to each HERV-K locus and applies mixture model-based clustering of these values to account for low depth sequence data. Our analysis of 1000 Genomes Project Data (KGP) reveals numerous differences among the five KGP super-populations in the prevalence of individual and co-occurring HERV-K proviruses; we provide a visualization tool to easily depict the proportion of the KGP populations with any combination of polymorphic HERV-K provirus. Further, because HERV-K is insertionally polymorphic, the genome burden of known polymorphic HERV-K is variable in humans; this burden is lowest in East Asian (EAS) individuals. Our study identifies population-specific sequence variation for HERV-K proviruses at several loci. We expect these resources will advance research on HERV-K contributions to human diseases.
Anthropogenic CO2 emissions from fossil fuel combustion and industrial processes are considered as the major contributors to global warming and climate change. Deploying carbon capture, storage and ...utilization (CCUS) has been perceived as one important strategy to mitigate CO2 emission and to realize carbon neutrality. Dry potassium-based adsorbents with desirable attributes show promise for capturing CO2 from flue gas and other ultra-dilute gas stream. The past decade has witnessed rapid development in dry potassium-based CO2 adsorbents. This review summarizes the recent progress of potassium-based adsorbents in CO2 capture. A detailed and in-depth discussion concerning the carbonation pathways is presented. Strategies for carbonation enhancement are demonstrated from the perspective of the rational design of adsorbents and process optimizations. Kinetics modelling of the carbonation and regeneration behaviors of potassium-based adsorbents is then illustrated. Apart from flue gas cleaning, the potential applications of potassium-based adsorbents in other fields such as low-concentration CO2 removal from environmental control and life support systems (ECLSSs), direct air capture (DAC), and integrated CO2 capture and utilization (ICCU) have also been discussed. Finally, comments on the main challenges and future opportunities of the dry potassium-based adsorbents are provided.
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