Individuals with sickle cell disease are increasingly surviving into adulthood, many of whom have interest in future biological parenthood. Reproductive health knowledge is low among adolescent and ...young adult males and their caregivers. Their understanding of these topics is needed to optimize their reproductive health outcomes. As such, through collaboration with a community advisory board (adolescents and young adults with sickle cell disease and mothers of adolescent and young adult males with sickle cell disease) and digital design team, we developed a web-based sickle cell disease-focused reproductive health program entitled FUTURES to address these knowledge gaps. For phase I of this two phase feasibility and acceptability study, adolescent and young adult males and their caregivers will complete a pre- and post-program reproductive health knowledge and attitudes questionnaire to assess change in knowledge. In phase II, after learning about fertility testing as part of the FUTURES curriculum, adolescent and young adult male participants are given the option to pursue testing. The two-phase study aims to: 1) develop and test the feasibility, acceptability, and efficacy of a reproductive health web-based educational program at increasing reproductive health knowledge in male adolescent and young adult males with sickle cell disease and their caregivers, and 2) assess feasibility of fertility testing. The long-term goal is to improve reproductive and psychosocial outcomes among adolescent and young adult males with sickle cell disease.
The microbial potential for toluene degradation within sediments from a tar oil-contaminated site in Flingern, Germany, was assessed using a metagenomic approach. High molecular weight environmental ...DNA from contaminated sediments was extracted, purified, and cloned into fosmid and BAC vectors and transformed into
Escherichia coli
. The fosmid library was screened by hybridization with a PCR amplicon of the α-subunit of the toluene 4-monooxygenase gene to identify genes and pathways encoding toluene degradation. Fourteen clones were recovered from the fosmid library, among which 13 were highly divergent from known
tmoA
genes and several had the closest relatives among
Acinetobacter
species. The BAC library was transferred to the heterologous hosts
Cupriavidus metallidurans
(phylum Proteobacteria) and
Edaphobacter aggregans
(phylum Acidobacteria). The resulting libraries were screened for expression of toluene degradation in the non-degradative hosts. From expression in
C. metallidurans
, three novel toluene monooxygenase-encoding operons were identified that were located on IncP1 plasmids. The
E. aggregans
-hosted BAC library led to the isolation of a cloned genetic locus putatively derived from an Acidobacteria taxon that contained genes involved in aerobic and anaerobic toluene degradation. These data suggest the important role of plasmids in the spread of toluene degradative capacity and indicate putative novel
tmoA
genes present in this hydrocarbon-polluted environment.
•Military air battle managers must make a sequence of tasking and routing decisions•A high-value asset must be defended from arriving salvos of cruise missiles•An air battle management problem is ...solved via approximate dynamic programming•Our modeling & solution procedures improve behavior of defensive counter-air forces•Trajectory-following state sampling and regularization are key algorithm features
Military air battle managers face several challenges when directing operations during quickly evolving combat scenarios. These scenarios require rapid assignment decisions to engage moving targets having dynamic flight paths. In defensive operations, the success of a sequence of air battle management decisions is reflected by the friendly force’s ability to maintain air superiority and defend friendly assets. We develop a Markov decision process (MDP) model of a stochastic dynamic assignment problem, named the Air Battle Management Problem (ABMP), wherein a set of unmanned combat aerial vehicles (UCAV) must defend an asset from cruise missiles arriving stochastically over time. Attaining an exact solution using traditional dynamic programming techniques is computationally intractable. Hence, we utilize an approximate dynamic programming (ADP) technique known as approximate policy iteration with least squares temporal differences (API-LSTD) learning to find high-quality solutions to the ABMP. We create a simulation environment in conjunction with a generic yet representative combat scenario to illustrate how the ADP solution compares in quality to a reasonable, closest-intercept benchmark policy. Our API-LSTD policy improves mean success rate by 2.8% compared to the benchmark policy and offers an 81.7% increase in the frequency with which the policy performs perfectly. Moreover, we find the increased success rate of the ADP policy is, on average, equivalent to the success rate attained by the benchmark policy when using a 20% faster UCAV. These results inform military force management and defense acquisition decisions and aid in the development of more effective tactics, techniques, and procedures.
Coral populations have precipitously declined on Caribbean reefs while algal abundance has increased, leading to enhanced competitive damage to corals, which likely is mediated by the potent ...allelochemicals produced by both macroalgae and benthic cyanobacteria. Allelochemicals may affect the composition and abundance of coral-associated microorganisms that control host responses and adaptations to environmental change, including susceptibility to bacterial diseases. Here, we demonstrate that extracts of six Caribbean macroalgae and two benthic cyanobacteria have both inhibitory and stimulatory effects on bacterial taxa cultured from the surfaces of Caribbean corals, macroalgae, and corals exposed to macroalgal extracts. The growth of 54 bacterial isolates was monitored in the presence of lipophilic and hydrophilic crude extracts derived from Caribbean macroalgae and cyanobacteria using 96-well plate bioassays. All 54 bacterial cultures were identified by ribotyping. Lipophilic extracts from two species of
Dictyota
brown algae inhibited >50% of the reef coral bacteria assayed, and hydrophilic compounds from
Dictyota menstrualis
particularly inhibited
Vibrio
bacteria, a genus associated with several coral diseases. In contrast, both lipo- and hydrophilic extracts from 2 species of
Lyngbya
cyanobacteria strongly stimulated bacterial growth. The brown alga
Lobophora variegata
produced hydrophilic compounds with broad-spectrum antibacterial effects, which inhibited 93% of the bacterial cultures. Furthermore, bacteria cultured from different locations (corals vs. macroalgae vs. coral surfaces exposed to macroalgal extracts) responded differently to algal extracts. These results reveal that extracts from macroalgae and cyanobacteria have species-specific effects on the composition of coral-microbial assemblages, which in turn may increase coral host susceptibility to disease and result in coral mortality.
Surface plasmon resonance (SPR) imaging is a surface-sensitive spectroscopic technique for measuring interactions between unlabeled biological molecules with arrays of surface-bound species. In this ...paper, SPR imaging is used to quantitatively detect the hybridization adsorption of short (18-base) unlabeled DNA oligonucleotides at low concentration, as well as, for the first time, the hybridization adsorption of unlabeled RNA oligonucleotides and larger 16S ribosomal RNA (rRNA) isolated from the microbe Escherichia coli onto a DNA array. For the hybridization adsorption of both DNA and RNA oligonucleotides, a detection limit of 10 nM is reported; for large (1500-base) 16S rRNA molecules, concentrations as low as 2 nM are detected. The covalent attachment of thiol-DNA probes to the gold surface leads to high surface probe density (1012 molecules/cm2) and excellent probe stability that enables more than 25 cycles of hybridization and denaturing without loss in signal or specificity. Fresnel calculations are used to show that changes in percent reflectivity as measured by SPR imaging are linear with respect to surface coverage of adsorbed DNA oligonucleotides. Data from SPR imaging is used to construct a quantitative adsorption isotherm of the hybridization adsorption on a surface. DNA and RNA 18-mer oligonucleotide hybridization adsorption is found to follow a Langmuir isotherm with an adsorption coefficient of 1.8 × 107 M-1.
Sea turtles are vulnerable to climate change since their reproductive output is influenced by incubating temperatures, with warmer temperatures causing lower hatching success and increased ...feminization of embryos. Their ability to cope with projected increases in ambient temperatures will depend on their capacity to adapt to shifts in climatic regimes. Here, we assessed the extent to which phenological shifts could mitigate impacts from increases in ambient temperatures (from 1.5 to 3°C in air temperatures and from 1.4 to 2.3°C in sea surface temperatures by 2100 at our sites) on four species of sea turtles, under a “middle of the road” scenario (SSP2‐4.5). Sand temperatures at sea turtle nesting sites are projected to increase from 0.58 to 4.17°C by 2100 and expected shifts in nesting of 26–43 days earlier will not be sufficient to maintain current incubation temperatures at 7 (29%) of our sites, hatching success rates at 10 (42%) of our sites, with current trends in hatchling sex ratio being able to be maintained at half of the sites. We also calculated the phenological shifts that would be required (both backward for an earlier shift in nesting and forward for a later shift) to keep up with present‐day incubation temperatures, hatching success rates, and sex ratios. The required shifts backward in nesting for incubation temperatures ranged from −20 to −191 days, whereas the required shifts forward ranged from +54 to +180 days. However, for half of the sites, no matter the shift the median incubation temperature will always be warmer than the 75th percentile of current ranges. Given that phenological shifts will not be able to ameliorate predicted changes in temperature, hatching success and sex ratio at most sites, turtles may need to use other adaptive responses and/or there is the need to enhance sea turtle resilience to climate warming.
Sea turtles are vulnerable to climate change and their ability to cope with projected increases in temperatures will depend on their capacity to adapt to shifts in climatic regimes. We assessed the extent to which phenological shifts could mitigate impacts from increases in temperatures on four species of sea turtles. Expected shifts in nesting will not be sufficient to maintain current incubation temperatures at 7 (29%) of our sites, hatching success rates at 10 (42%) of our sites, with current trends in hatchling sex ratio being able to be maintained at half of the sites.
Three groups of Aeromonas strains isolated from Finland lakes experiencing cyanobacterial blooms could not be assigned to any known species of this genus on the basis of 16S rRNA and rpoD gene ...sequences. The Multilocus Phylogenetic Analysis (MLPA) of the concatenated sequence of seven genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX and atpD; 4093bp) showed that the three groups of strains did not cluster with any known Aeromonas spp. and formed three independent lineages. This was confirmed by performing the analysis with their closest relatives using 15 genes (the latter 7 and cpn60, dnaK, gltA, mdh, radA, rpoB, tsf, zipA; 8751bp). Furthermore, ANI results between the genomes of the type strains of the three potential new species and those of their close relatives were all <96% which is the previously proposed cutoff value for differentiating species within this genus. The in silico DDH values of the three type strains of the new species also showed a similarity <70% with the most closely related species indicating they belong to different taxa. The three groups of strains could be differentiated from each other and from other known Aeromonas species on the basis of several phenotypic characters. This polyphasic study revealed that the 3 groups of strains represent 3 novel Aeromonas species for which the names Aeromonas aquatica sp. nov. (type strain AE235T=CECT 8025T=LMG 26712T), Aeromonas finlandiensis sp. nov. (type strain 4287DT=CECT 8028T=LMG 26709T) and Aeromonas lacus sp. nov. (type strain AE122T=CECT 8024T=LMG 26710T) are proposed.
Bruce A. Glasrud and Deborah M. Liles have gathered over thirty years of scholarship—articles, book excerpts, and new, original essays—to offer for the first time an overview of the ...history of African Americans in Central Texas. From slavery and agriculture in the nineteenth century to entrepreneurship and the struggle for civil rights in the twentieth century, African Americans in Central Texas History: From Slavery to Civil Rights fills in the critical missing pieces of an often-overlooked region in the state’s history. African Americans first entered Central Texas with Spanish explorers, but few remained. White slave holders later brought black residents—as slaves—to this region. With the end of the Civil War, slavery may have ended but the brutalities of racial prejudice persisted. During Reconstruction, new attempts to ensure civil and political rights were resisted through terror, racial violence, and systemic denial of justice. Well into the twentieth century, segregation persisted, but years of individual and mobilized protest finally led to significant reform. Organizations such as the NAACP provided vital support. Before efforts to disenfranchise the black vote became successful, some politicians even courted black voters to further their own political agendas. African Americans in Central Texas History is a rare source that sheds light on the African American experience in the heart of the state.
Abstract Four Aeromonas strains from clinical and environmental samples differed from known species on the basis of rpoD gene sequence. Multilocus phylogenetic analysis and in silico DNA-DNA ...hybridization confirmed them as four new species even though their 16S rRNA gene sequence similarity with their closest relatives was >98.7%, as occurred for other Aeromonas spp.
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