Periodontitis is an immuno-inflammatory disease, which can lead to tooth loss. This study aimed to investigate the efficacy of Platymiscium floribundum Vog., a Brazilian tree which has been used in ...folk medicine as an anti-inflammatory agent, in a pre-clinical trial of periodontitis in rats. Periodontitis was induced by placing a sterilized nylon (3.0) thread ligature around the cervix of the second left upper molar of the rats, which received (per os) P. floribundum extract (0.1, 1 or 10 mg/kg) or vehicle 1h before periodontitis-challenge and once daily during 11 days. Treatment with P. floribundum (10mg/kg) decreased alveolar bone loss, MPO activity nitrite/nitrate levels, oxidative stress, TNF-α, IL1-β, IL-8/CINC-1, and PGE2 gingival levels, and transcription of TNF-α, IL1-β, COX-2, iNOS, RANK, and RANKL genes, while elevated both BALP serum levels and IL-10 gingival levels. The animals did not show signs of toxicity throughout the experimental course. These findings show that P. floribundum has anti-inflammatory and anti-resorptive properties in a pre-clinical trial of periodontitis, representing an interesting biotechnological tool.
MS is an autoimmune demyelinating disease of the CNS, which causes neurologic deficits in young adults and leads to progressive disability. The aryl hydrocarbon receptor (AHR), a ligand-activated ...transcription factor, can drive anti-inflammatory functions in peripheral immune cells and also in CNS-resident cells. Laquinimod is a drug developed for the treatment of MS known to activate AHR, but the cellular targets of laquinimod are still not completely known. In this work, we analyzed the contribution of AHR activation in astrocytes to its beneficial effects in the experimental autoimmune encephalomyelitis (EAE) preclinical model of MS.
We used conditional knockout mice, in combination with genome-wide analysis of gene expression by RNA-seq and in vitro culture systems to investigate the effects of laquinimod on astrocytes.
We found that AHR activation in astrocytes by laquinimod ameliorates EAE, a preclinical model of MS. Genome-wide RNA-seq transcriptional analyses detected anti-inflammatory effects of laquinimod in glial cells during EAE. Moreover, we established that the Delaq metabolite of laquinimod dampens proinflammatory mediator production while activating tissue-protective mechanisms in glia.
Taken together, these findings suggest that AHR activation by clinically relevant AHR agonists may represent a novel therapeutic approach for the treatment of MS.
Abstract
Introduction
A good doctor-patient relationship is crucial to medical practice. A fundamental element that drives this relationship is the empathy of the treating physician, which has also ...been validated as a predictor of clinical competence and patient results.
Purpose
We conducted an original study to correlate empathy values of our physicians and patient satisfaction surveys. In this subanalysis, we evaluated whether age, sex or time since qualifying as specialist had an impact on empathy values.
Methods
In our originaly study we sought to correlate the empathy values of our physicians in our cardiovascular clinic with the results of outpatient satisfaction surveys, measured with HCAPS, using a linear regression model. The Jefferson Empathy scale is a self reported 20 question survey, answered through a 1-7 Likert scale, with a maximum score of 140 points. It is further subclassified into 3 categories: "Perspective", "Care with compassion" and "Stepping into patient's shoes". The HCAPS survey is a patient satisfaction query, with 5 items that inquire as to the patient's perception of being treated with respect by the physician, the interest shown in their problems, the time dedicated to the consult, and the quality of the explanation of their chief concern and treatment options. It is anwered through a Likert scale from "Totally diasgree" to "Totally agree". There is also a final question that asks for a global impression.
Results
65 doctors answered the Empathy survey, 30.8% of them women. Mean age was 48.8 years, and over half were under 50 years old. 81% had been practicing for over 5 years. 61671 HCAPS surveys were analyzed, corresponding to those 65 practitioners. Mean Empathy score was 120.7 (DS 11.1). We found that patients perceived greater respectful treatment (p=0.03) and had their treatment options better explained to them (p=0.02) by physicians with higher levels of empathy. The other HCAPS queries had a positive, though non statistically significant, relationship with Empathy scores. Doctors with higher scores in the subcategory "stepping into patient's shoes" had a significant increase in patient's perception of the interest shown in their concerns (p=0.01). There were no differences in physicians' empathy levels according to their age, gender, or time since qualifying as a specialist.
Discussion
These results validate empathy as a key skill in the doctor-patient relationship, and show that high levels of this personality attribute lead to better patient satisfaction results. Our results differ from other published work in that we found no difference in empathy levels according to gender or practice time, whereas most previous literature found that women and longer practice time were positively associated with empathy levels.
Lycopene is a natural pigment synthesized by plants and microorganisms, and it is mainly found in tomatoes. It is an acyclic isomer of β-carotene and one of the most potent antioxidants. Several ...studies have demonstrated the ability of lycopene to prevent chemically induced DNA damage; however, the mechanisms involved are still not clear. In the present study, we investigated the antigenotoxic/antimutagenic effects of lycopene in Chinese Hamster Ovary Cells (CHO) treated with hydrogen peroxide, methylmethanesulphonate (MMS), or 4-nitroquinoline-1-oxide (4-NQO). Lycopene (97%), at final concentrations of 10, 25, and 50
μM, was tested under three different protocols: before, simultaneously, and after the treatment with the mutagens. Comet and cytokinesis-block micronucleus assays were used to evaluate the level of DNA damage. Data showed that lycopene reduced the frequency of micronucleated cells induced by the three mutagens. However, this chemopreventive activity was dependent on the concentrations and treatment schedules used. Similar results were observed in the comet assay, although some enhancements of primary DNA damage were detected when the carotenoid was administered after the mutagens. In conclusion, our findings confirmed the chemopreventive activity of lycopene, and showed that this effect occurs under different mechanisms.
This study conducts a comparative electrochemical evaluation of three types of pearlitic steels used in flexible pipelines for oil transport in marine environments. The steels have been manufactured ...with chemical composition and geometry variations to optimize operation performance under adverse conditions. Electrochemical tests were conducted using solutions simulating marine environments with NaCl and CO2, and at high temperatures. The results indicated that spheroidized (SC) steel demonstrated the best corrosion resistance under these specific conditions. Additionally, the Raman spectroscopy characterization technique was used to analyze the layers of corrosion products formed during the tests, identifying the presence of FeCO3 (siderite) and other corrosive oxides. These discoveries are valuable for selecting and improving materials in flexible pipelines used in oil production in marine waters. The study highlights the importance of the cementite morphology present in pearlite as a relevant factor in the corrosive behavior of steels, contributing to the development of more efficient and durable solutions for the offshore oil and gas industry.
Nitazoxanide (NTZ) is a broad-spectrum anti-parasitic drug used against a wide variety of protozoans and helminthes. Albendazole, its active metabolite albendazole sulfoxide (ABZSO), is one of the ...drugs of choice to treat both intestinal and tissue helminth and protozoan infections. However little is known regarding their impact on the metabolism of parasites. The aim of this study was to compare the in vitro effect of NTZ and ABZSO in the glycolysis of Taenia crassiceps cysticerci. The cysticerci were treated with 1.2; 0.6; 0.3 or 0.15 μg/mL of NTZ or ABZSO. Chromatographic and spectrophotometric analyses were performed in the culture medium and in the cysticerci extract. Regarding the glucose concentrations was possible to observe two responses: impair of the uptake and gluconeogenesis. The pyruvate concentrations were increased in the ABZSO treated group. Lactate concentrations were increased in the culture medium of NTZ treated groups. Therefore it was possible to infer that the metabolic acidosis was greater in the group treated with NTZ than in the ABZSO treated group indicating that this is one of the modes of action used by this drug to induce the parasite death.
Lactate concentrations in the culture medium and in the cysticerci extract of cysticerci exposed to different concentrations of albendazole and nitazoxanide. CM: concentrations detected in the culture medium analysis; CYST: concentrations detected in the cysticerci extract; CTL CM: RPMI culture medium without cysticerci; CTL CYST: cysticerci without drugs exposure; NTZ 1.2: cysticerci exposed to 1.2 μg/mL of nitazoxanide; NTZ 0.6: cysticerci exposed to 0.6 μg/mL of nitazoxanide; NTZ 0.3 cysticerci exposed to 0.3 μg/mL of nitazoxanide; NTZ 0.15: cysticerci exposed to 0.15 μg/mL of nitazoxanide; ABZSO 1.2: cysticerci exposed to 1.2 μg/mL of albendazole sulfoxide; ABZSO 0.6: cysticerci exposed to 0.6 μg/mL of albendazole sulfoxide; ABZSO 0.3: cysticerci exposed to 0.3 μg/mL of albendazole sulfoxide; ABZSO 0.15: cysticerci exposed to 0.15 μg/mL of albendazole sulfoxide. * statistical difference when compared to the control group. Display omitted
•NTZ induces impair in glucose uptake in in vitro cysticerci.•NTZ did not influence the pyruvate concentrations in in vitro cysticerci.•NTZ induces metabolic acidosis in in vitro cysticerci.•The metabolic acidosis in vitro induced by NTZ is greater than ABZ.
The goal of the this study was to evaluate the mutagenic/antimutagenic effects of conventional (BRS133) and transgenic (BRS 245 RR) soybeans (CS and TS, respectively) in vivo using the bone marrow ...micronucleus (MN) test, histopathological analysis, chromosome aberration test (CAT), and mitotic index (MI) determination. Six-week-old male Swiss mice were fed with pelleted commercial diet mixed with CS or TS at 10% or 20%. Two experimental designs (MN and CAT) were conducted simultaneously with 10 groups each during a 15-day period. Animals were treated with pelleted commercial diet, CS (10% or 20%), or TS (10% or 20%), and on day 14 they also received cyclophosphamide (CP) (50 mg/kg i.p.). The 10% and 20% CS and TS diets did not significantly decrease the frequencies of micronucleated polychromatic erythrocytes in bone marrow induced by CP. However, the CAT indicated that the 10% and 20% CS diets significantly (P < .05) protected nucleated bone marrow cells against chemical-induced mutagenesis and also produced a significant (P < .05) decrease in the total percentage of spontaneous aberrations. Among the treatments with TS, only the 10% TS diet reduced the percentage of total aberrations induced by CP. The results also indicated that the treatment with 20% TS alone significantly (P < .05) decreased the MI, indicating cytotoxic effects related to the treatment. Taken together, our results suggest that, under the tested conditions, TS and CS have antimutagenic properties and are not toxic.
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