This paper first analyzes the K-mean algorithm from the core idea, algorithm process and advantages and disadvantages, then further improves the K-mean algorithm by using Gaussian mixture ...distribution and constructs the skill-based street dance movement recognition model based on the improved algorithm. Finally, the street dance teaching video is used as an example for dance movement acquisition and data pre-processing, and the recognition accuracy analysis of the street dance movement dataset is conducted based on the improved K-mean algorithm. The average recognition rates of the recognition model in the four data sets of the data set were 72.34%, 74.65%, 73.15% and 86.70%, respectively. This shows that analyzing the characteristics of street dance movements using the improved K-mean algorithm is beneficial for optimizing and improving existing street dance movements.
The plant aluminum (Al)-activated malate transporter ALMT1 mediates the efflux of malate to chelate the Al in acidic soils and underlies the plant Al resistance. Here we present cryo-electron ...microscopy (cryo-EM) structures of Arabidopsis thaliana ALMT1 (AtALMT1) in the apo, malate-bound, and Al-bound states at neutral and/or acidic pH at up to 3.0 Å resolution. The AtALMT1 dimer assembles an anion channel and each subunit contains six transmembrane helices (TMs) and six cytosolic α-helices. Two pairs of Arg residues are located in the center of the channel pore and contribute to malate recognition. Al binds at the extracellular side of AtALMT1 and induces conformational changes of the TM1-2 loop and the TM5-6 loop, resulting in the opening of the extracellular gate. These structures, along with electrophysiological measurements, molecular dynamic simulations, and mutagenesis study in Arabidopsis, elucidate the structural basis for Al-activated malate transport by ALMT1.
The effects of transgenically encoded human and mouse IL-18 on T cell proliferation and its application in boosting chimeric antigen receptor (CAR) T cells are presented. Robust enhancement of ...proliferation of IL-18-secreting human T cells occurred in a xenograft model, and this was dependent on TCR and IL-18R signaling. IL-18 augmented IFN-γ secretion and proliferation of T cells activated by the endogenous TCR. TCR-deficient, human IL-18-expressing CD19 CAR T cells exhibited enhanced proliferation and antitumor activity in the xenograft model. Antigen-propelled activation of cytokine helper ensemble (APACHE) CAR T cells displayed inducible expression of IL-18 and enhanced antitumor immunity. In an intact mouse tumor model, CD19-IL-18 CAR T cells induced deeper B cell aplasia, significantly enhanced CAR T cell proliferation, and effectively augmented antitumor effects in mice with B16F10 melanoma. These findings point to a strategy to develop universal CAR T cells for patients with solid tumors.
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•Augmented proliferation of synthetic IL-18-expressing human T cells•rIL-18 augments IFN-γ secretion and proliferation of anti-CD3 activated T cells•IL-18-secreting CD4+ T cells promote CD8+ T cells through a helper effect•IL-18 CAR T cells have superior proliferation and antitumor activity in mouse models
Hu et al. create IL-18-secreting chimeric antigen receptor T (IL-18-CAR T) cells to significantly boost CAR T cell proliferation and antitumor activity.
An actinomycete strain, CB-75, was isolated from the soil of a diseased banana plantation in Hainan, China. Based on phenotypic and molecular characteristics, and 99.93% sequence similarity with
NBRC ...13424 (AB184393), the strain was identified as
sp. This strain exhibited broad-spectrum antifungal activity against 11 plant pathogenic fungi. Type I polyketide synthase (PKS-I) and non-ribosomal peptide synthetase (NRPS) were detected, which were indicative of the antifungal compounds that
sp. CB-75 could produce. An ethyl acetate extract from the strain exhibited the lowest minimum inhibitory concentration (MIC) against
(ATCC 96167) (0.78 μg/ml) and yielded the highest antifungal activity against
(ATCC 16330) (50.0 μg/ml). Also, spore germination was significantly inhibited by the crude extract. After treatment with the crude extract of
sp. CB-75 at the concentration 2 × MIC, the pathogenic fungi showed deformation, shrinkage, collapse, and tortuosity when observed by scanning electron microscopy (SEM). By gas chromatography-mass spectrometry (GC-MS) of the crude extract, 18 chemical constituents were identified; (
)-13-docosenamide was the major constituent. Pot experiments showed that the incidence of banana seedlings was reduced after using
sp. CB-75 treatment. The disease index was 10.23, and the prevention and control effect was 83.12%. Furthermore,
sp. CB-75 had a growth-promoting effect on banana plants. The chlorophyll content showed 88.24% improvement, the leaf area, root length, root diameter, plant height, and stem showed 88.24, 90.49, 136.17, 61.78, and 50.98% improvement, respectively, and the shoot fresh weight, root fresh weight, shoot dry weight, and root dry weight showed 82.38, 72.01, 195.33, and 113.33% improvement, respectively, compared with treatment of fermentation broth without
sp. CB-75. Thus,
sp. CB-75 is an important microbial resource as a biological control against plant pathogenic fungi and for promoting banana growth.
Melaleuca bracteata is a yellow-leaved tree belonging to the Melaleuca genus. Species from this genus are known to be good sources of natural antioxidants, for example, the "tea tree oil" derived ...from M. alternifolia is used in food processing to extend the shelf life of products. In order to determine whether M. bracteata contains novel natural antioxidants, the components of M. bracteata ethanol extracts were analyzed by gas chromatography-mass spectrometry. Total phenolic and flavonoid contents were extracted and the antioxidant activities of the extracts evaluated. Single-factor experiments, central composite rotatable design (CCRD) and response surface methodology (RSM) were used to optimize the extraction conditions for total phenolic content (TPC) and total flavonoid content (TFC). Ferric reducing power (FRP) and 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH·) scavenging capacity were used as the evaluation indices of antioxidant activity. The results showed that the main components of M. bracteata ethanol extracts are methyl eugenol (86.86%) and trans-cinnamic acid methyl ester (6.41%). The single-factor experiments revealed that the ethanol concentration is the key factor determining the TPC, TFC, FRP and DPPH·scavenging capacity. RSM results indicated that the optimal condition of all four evaluation indices was achieved by extracting for 3.65 days at 53.26°C in 34.81% ethanol. Under these conditions, the TPC, TFC, FRP and DPPH·scavenging capacity reached values of 88.6 ± 1.3 mg GAE/g DW, 19.4 ± 0.2 mg RE/g DW, 2.37 ± 0.01 mM Fe2+/g DW and 86.0 ± 0.3%, respectively, which were higher than those of the positive control, methyl eugenol (FRP 0.97 ± 0.02 mM, DPPH·scavenging capacity 58.6 ± 0.7%) at comparable concentrations. Therefore, the extracts of M. bracteata leaves have higher antioxidant activity, which did not only attributed to the methyl eugenol. Further research could lead to the development of a potent new natural antioxidant.
The study of the structure and properties of extractants is of great significance for developing new extractants. For uranium extraction and recovery requirements in spent fuel reprocessing, three ...kinds of extractants with different ether-oxygen chains,
N,N,N’N’
-tetraoctyl-3-oxapentanediamide (TODGA),
N,N,N’N’-
tetraoctyl-3,6-dioxaoctanediamide (TODOODA) and
N,N,N’N’
-tetraoctyl-3,6,9-trioxaundecanediamide (TOUDA) were synthesized to study the influence of the ether-oxygen chain skeleton on the extraction of uranium from nitrate media. TODOODA shows a good extraction ability for uranyl ions at high nitric acid concentrations. Equimolar series method, FT–IR spectrum and MALDI–TOF–MS spectra indicated that the extracted structure was formed as UO
2
NO
3
·TODGANO
3
, UO
2
NO
3
·TODOODANO
3
and UO
2
NO
3
·TOUDANO
3
.
In China, the spread and outbreak of OXA-48-producing Enterobacteriaceae remains largely unknown.
OXA-48-producing isolates were analyzed for genetic relatedness by pulsed-field gel electrophoresis ...(PFGE), antimicrobial susceptibility by E-test, and sequence type (ST) by multilocus sequence typing. S1-PFGE and southern blotting were used for plasmid profiling, and PCR and subsequent sequencing were performed to determine the genetic environment of blaOXA-48 gene.
In total, 37 non-duplicated OXA-48-producing K. pneumoniae (OXAKp) isolates were recovered. From December 2013 to August 2014, an outbreak was observed at a respiratory ICU. The 37 isolates of K. pneumoniae were categorized into four PFGE types (A, B, C, and D). The predominant strains associated with the outbreak were strains with PFGE type A and B, which belonged to ST383 and ST147, respectively. Plasmid sequencing revealed that the blaOXA-48-carrying plasmid is 69,069 bp in length and belongs to the IncL/M incompatibility group. Sequence analysis revealed that the IS1999 element was located upstream of the blaOXA-48 gene and was truncated by IS1R.
In this study, the dissemination and outbreak of OXAKp isolates were clonal, and ST147 and ST383 K. pneumoniae were the predominant clones that were associated with the outbreak. Meanwhile, the horizontal transfer of plasmids potentially mediate the spread of blaOXA-48 gene between different K. pneumoniae strains.
Over the past few years, electrocatalysis for the oxygen reduction reaction in alkaline solutions has undergone tremendous advances, and non-precious metal catalysts are of prime interest. In this ...study, we present a highly promising CoO@Co/N-C （where N-C represents a N-doped carbon material） catalyst, achieving an onset potential of 0.99 V （versus the reversible hydrogen electrode （RHE）） and a limiting current density of 7.07 mA-cm-2 （at 0.3 V versus RHE） at a rotation rate of 2,500 rpm in an O2-saturated 0.1 M KOH solution, comparable to a commercial Pt/C catalyst. The H2--O2 alkaline fuel cell test of CoO@Co/N-C as the cathode reveals a maximum power density of 237 mW.cm 2. Detailed investigation clarifies that a synergistic effect, induced by C-N, Co-N-C, and CoO/Co moieties, is responsible for the bulk of the gain in catalytic activity.
Smart sports teaching is regarded as a new type of education mode, aiming to improve the quality and effect of sports teaching through the use of artificial intelligence technology. The article first ...combined the opportunities for digital transformation of college sports teaching given by AI technology, combined the application scenarios of AI technology to innovative college sports teaching, and established a smart sports teaching platform with smart IoT architecture. The OpenPose algorithm is utilized to extract the key points of the skeletal movements of sports teaching, and the optimal matching planning is carried out by the DTW algorithm combined with the ICP algorithm for the movement postures of sports. For the effectiveness of the algorithm, corresponding identification verification experiments were designed, and teaching comparison experiments were created on the intelligent sports teaching platform. The OpenPose algorithm spent only 0.284s in acquiring the skeletal key points of sports, and the average success rate of matching the movement postures of sports was 89.75%. There was a maximum difference of 10.81 points in the stage movement assessment between students of class A and class B, and the negative interest of students of class A in physical education was reduced by 40.97%. Negative interest decreased by 40.97%. Relying on the intelligent sports teaching platform and combining artificial intelligence technology to technologically upgrade sports teaching in colleges and universities provides a new path to enhance the quality of sports teaching in colleges and universities.
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