•Prepared composite nanofiber membrane possesses a good photocatalyst performance.•High degradation activity of organic dyes under visible light.•The composite nanofibers membrane possessed excellent ...recovery and reuse.•The free-standing membrane is flexible and easy to handle.•The flexibility of the composite nanofibers allows use in a continuous operation.
This study proposed an effective method by using photocatalytic composite nanofiber membranes in order to degrade malachite green (MG) and acid red 27 (AR 27) to harmless products. The photocatalytic composite nanofiber membrane, Polyacrylonitrile (PAN) was incorporated with catalyst SiO2 and TiO2 photocatalyst. The PAN/SiO2-TiO2-NH2 nanofiber membrane was synthesized by electrospinning technique followed by chemical crosslinking. The results of SEM, EDX, FTIR and XRD confirmed the successful preparation of nanofiber membrane. XRD analysis shows the confirmation of SiO2 and TiO2 peaks. The result shows that PAN/SiO2-TiO2-NH2 nanofiber membrane exhibit the highest degradation with a good distribution of SiO2 and TiO2 on the PAN nanofiber membrane surface. The results showed that the complete photodegradation of MG and AR 27 with artificial visible-light was achieved after 9 and 25 min, respectively. In addition, the nanofiber membrane exhibited good stability and reusability for MG and AR 27 photodegradation and has potential to be applied in industrial application.
The ineffectiveness of treatments for infections caused by biofilm-producing pathogens and human carcinoma presents considerable challenges for global public health organizations. To tackle this ...issue, our study focused on exploring the potential of synthesizing new complexes of Co(II), Cu(II), Ni(II), and Zn(II) with sorbic acid to enhance its antibacterial, antibiofilm, and anticancer properties. Four novel complexes were synthesized as solid phases by reacting sorbic acid with Co(II), Cu(II), Ni(II), and Zn(II). These complexes were characterized by various technique, including infrared spectra, UV-Visible spectroscopy, proton nuclear magnetic resonance (1H NMR), and thermal analysis techniques, including thermogravimetry (TG). The data acquired from all investigated chemical characterization methods confirmed the chemical structure of the sorbate metal complexes. These complexes exhibited antibacterial and antibiofilm properties against both Gram-positive and Gram-negative bacteria. Furthermore, these complexes enhanced the antibacterial effects of commonly used antibiotics, such as gentamicin and imipenem, with fractional inhibitory concentration (FIC) indices less than or equal to 0.5. Notably, the Cu(II) complex displayed the most potent antibacterial and antibiofilm activities, with minimum inhibitory concentration (MIC) values of 312.5 microg/mL and 625.0 microg/mL for Bacillus cereus and Escherichia coli, respectively. Additionally, in vitro assays using the methyl thiazolyl tetrazolium (MTT) method showed inhibitory effects on the growth of the human colon carcinoma cell line (HCT-116 cells) following treatment with the investigated metal complexes. The IC50 values for Co(II), Cu(II), Zn(II), and Ni(II) were 3230 microg/mL, 2110 microg/mL, 3730 microg/mL, and 2240 microg/mL, respectively. Our findings offer potential for pharmaceutical companies to explore the development of novel combinations involving traditional antibiotics or anticancer drugs with sorbate copper complex.
Although different products have been developed to prevent post-surgical adhesion, their efficacy remains unsatisfactory. This study aimed to evaluate the efficacy of the gelatin sponge in the ...prevention of post-surgical intra-abdominal adhesions in a rat model. Rats were randomly divided into sham, adhesion, and gelatin groups. All rats, except the sham group, underwent cecal abrasion to establish an adhesion model. After celiotomy, a sterile gelatin sponge was applied intra-abdominal on the abraded cecum in the gelatin group. Rats were sacrificed on day 14 post-surgery and intra-abdominal adhesions were evaluated and scored. Adhesion tissues were evaluated by histological, histochemical, and immunohistochemical analysis. Intra-abdominal adhesions were recorded in all rats of the adhesion group. Intra-abdominal application of gelatin sponge significantly (P < 0.001) reduced intra-abdominal adhesions by 91% in the gelatin group relative to the adhesion group. The histological analysis revealed a marked decrease (P < 0.001) in the inflammatory score and neovascularization in the gelatin group. The histochemical analysis found that gelatin sponge administration reduced adhesion formation and thickness of adhesion tissue. Moreover, gelatin sponge significantly (P < 0.0001) increased MMP-9 expression and decreased macrophage marker expression in adhesive tissue. This study revealed that the application of gelatin sponge markedly reduced the post-surgical intra-abdominal adhesions and suggests new guidance for using gelatin sponge as an anti-adhesive substance in clinical practice.
•This study demonstrated the efficacy of the gelatin sponge in preventing post-surgical intra-abdominal adhesions in a rat model.•Intra-abdominal application of gelatin sponge significantly (P < 0.001) reduced adhesions by 91% relative to the adhesion group.•The histological analysis revealed a marked (P< 0.001) decrease in the inflammatory score and neovascularization in the gelatin group.•Gelatin sponge significantly (P < 0.0001) increased MMP-9 expression and decreased macrophage marker expression in adhesive tissue.•Gelatin sponge markedly reduces the post-surgical intra-abdominal adhesions and suggests as an anti-adhesive substance in clinical practice.
Chromium is a hazardous compound from industrial processes, known for its toxicity, mutagenicity, teratogenicity, and carcinogenicity. Chemical methods are efficient but cost-effective alternatives ...with reduced sludge are sought. Electro-coagulation, utilizing low-cost iron plate electrodes, was explored for factual tannery wastewater treatment in this manuscript. Operating parameters such as initial chromium concentration, voltage, electrode number, operating time, agitation speed and current density has been studied to evaluate the treatment effeciency. Under optimal conditions (15 V, 0.4 mA/cm2, 200 rpm, 330 ppm chromium, 8 iron electrodes with a total surface area of 0.1188 m2, 3 h), chromium elimination was 98.76%. Iron anode consumption, power use, and operating cost were 0.99 gm/L, 0.0143 kW-h/L, and 160 EGP/kg of chromium eliminated, respectively. Kinetics studies were pursued first-order reaction (97.99% correlation), and Langmuir isotherms exhibited strong conformity (Langmuir R2: 99.99%). A predictive correlation for chromium elimination (R2: 97.97%) was developed via statistical regression. At HARBY TANNERY factory in Egypt, industrial sewage treatment achieved a final chromium disposal rate of 98.8% under optimized conditions.
The condensation of
o
-benzoyl benzoic acid and 4-aminoantipyrine resulted in the formation of novel Schiff base ligand (HL) with the IUPAC name 2-(((1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1
H
...-pyrazol-4-yl)imino)(phenyl)methyl)benzoic acid. The synthesized Schiff base ligand and its complexes with M(II)/(III) transition elements (Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II)) were characterized by elemental, magnetic susceptibility, molar conductivity, spectroscopic (
1
H NMR, mass, UV–visible, FTIR, ESR), thermal and X-ray powder diffraction. The data showed that the complexes had composition of the MHL type. The diffused reflectance spectra, magnetic susceptibility and ESR spectral data of the complexes confirm an octahedral geometry around metal ions. The thermal analysis data revealed the decomposition of the complexes in three to five successive decomposition steps within the temperature range of 30–1000°C, and the activation thermodynamic parameters were reported. The molecular structures of the Schiff base ligand and its Mn(II) and Zn(II) metal complexes are optimized theoretically, and the quantum chemical parameters are calculated. In order to predict the binding between
o
-benzoyl benzoic acid, 4-aminoantipyrine and HL ligand with the
Escherichia coli
bacterial RNA (4p20) receptor, molecular docking was carried out. The in vitro antimicrobial screening of the newly synthesized compounds was tested against different bacterial and fungal organisms. The results showed that the metal complexes have biologically activity more than the new Schiff base ligand against the tested organisms. The Schiff base ligand and its complexes were also screened for their anticancer activity against breast cancer cell line (MCF7). The Mn(II), Cr(III) and Cd(II) complexes were found to have low IC
50
values which support the possibility of using them as cytotoxic agents and hence might become good anticancer agent in clinical trials.
The condensation of o-benzoyl benzoic acid and 4-aminoantipyrine resulted in the formation of novel Schiff base ligand (HL) with the IUPAC name ...2-(((1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)imino)(phenyl)methyl)benzoic acid. The synthesized Schiff base ligand and its complexes with M(II)/(III) transition elements (Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II)) were characterized by elemental, magnetic susceptibility, molar conductivity, spectroscopic (.sup.1H NMR, mass, UV-visible, FTIR, ESR), thermal and X-ray powder diffraction. The data showed that the complexes had composition of the MHL type. The diffused reflectance spectra, magnetic susceptibility and ESR spectral data of the complexes confirm an octahedral geometry around metal ions. The thermal analysis data revealed the decomposition of the complexes in three to five successive decomposition steps within the temperature range of 30-1000°C, and the activation thermodynamic parameters were reported. The molecular structures of the Schiff base ligand and its Mn(II) and Zn(II) metal complexes are optimized theoretically, and the quantum chemical parameters are calculated. In order to predict the binding between o-benzoyl benzoic acid, 4-aminoantipyrine and HL ligand with the Escherichia coli bacterial RNA (4p20) receptor, molecular docking was carried out. The in vitro antimicrobial screening of the newly synthesized compounds was tested against different bacterial and fungal organisms. The results showed that the metal complexes have biologically activity more than the new Schiff base ligand against the tested organisms. The Schiff base ligand and its complexes were also screened for their anticancer activity against breast cancer cell line (MCF7). The Mn(II), Cr(III) and Cd(II) complexes were found to have low IC.sub.50 values which support the possibility of using them as cytotoxic agents and hence might become good anticancer agent in clinical trials.
Genus Canna is used in folk medicine as demulcent, diaphoretic, antipyretic, mild laxative and in gastrointestinal upsets therapy. Canna x generalis (CG) L.H. Bailey is traditionally used as ...anti-inflammatory, analgesic and antipyretic. Besides, CG is used in Ayurvedic medicines’ preparations and in the treatment of boils, wounds, and abscess. Nevertheless, its anti-inflammatory effects against ulcerative colitis (UC) are not yet investigated.
This study aimed to investigate the phytoconstituents of CG rhizome ethanol extract (CGE). Additionally, we aimed to comparatively evaluate its therapeutic effects and underlying mechanisms against the reference drug “sulphasalazine (SAS)” in dextran sodium sulfate (DSS)-induced UC in mice.
Metabolic profiling of CG rhizomes was performed via UHPLC/qTOF-HRMS; the total phenolic, flavonoid and steroid contents were determined, and the main phytoconstituents were isolated and identified. Next, DSS-induced (4%) acute UC was established in C57BL/6 mice. DSS-induced mice were administered either CGE (100 and 200 mg/kg) or SAS (200 mg/kg) for 7 days. Body weight, colon length, disease activity index (DAI) and histopathological alterations in colon tissues were examined. Colon levels of oxidative stress (GSH, MDA, SOD and catalase) and pro-inflammatory Myeloperoxidase (MPO), nitric oxide (NO), IL-1β, IL-12, TNF-α, and INF-γ markers were colourimetrically determined. Serum levels of lipopolysaccharide (LPS) and relative mRNA expressions of occludin, TLR4 and ASC (Apoptosis-Associated Speck-Like Protein Containing CARD) using RT-PCR were measured. Protein levels of NLRP3 inflammasome and cleaved caspase-1 were determined by Western blot. Furthermore, immunohistochemical examinations of caspase-3, NF-ҡB and claudin-1 were performed.
Major identified constituents of CGE were flavonoids, phenolic acids, phytosterols, beside five isolated phytoconstituents (β-sitosterol, triacontanol fatty alcohol, β-sitosterol-3-O-β-glucoside, rosmarinic acid, 6-O-p-coumaroyl-β-D-fructofuranosyl α-D-glucopyranoside). The percentage of the phenolic, flavonoid and steroid contents in CGE were 20.55, 6.74 and 98.09 μg of gallic acid, quercetin and β-sitosterol equivalents/mg extract, respectively. In DSS-induced mice, CGE treatment ameliorated DAI, body weight loss and colon shortening. CGE attenuated the DSS-induced colonic histopathological alternations, inflammatory cell infiltration and histological scores. CGE elevated GSH, SOD and catalase levels, and suppressed MDA, pro-inflammatory mediators (MPO and NO) as well as cytokines levels in colonic tissues. Moreover, CGE downregulated LPS/TLR4 signaling, caspase-3 and NF-ҡB expressions. CGE treatment inhibited NLRP3 signaling pathway as indicated by the suppression of the protein expression of NLRP3 and cleaved caspase-1, and the ASC mRNA expression in colonic tissues. Additionally, CGE restored tight junction proteins’ (occludin and claudin-1) expressions.
Our findings provided evidence for the therapeutic potential of CGE against UC. CGE restored intestinal mucosal barrier's integrity, mitigated oxidative stress, inflammatory cascade, as well as NF-ҡB/TLR4 and NLRP3 pathways activation in colonic tissues. Notably, CGE in a dose of 200 mg/kg was more effective in ameliorating DSS-induced UC as compared to SAS at the same dose.
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•DSS induces ulcerative colitis (UC) that resembles the pathological alternations in human UC.•Canna x generalis rhizome extract (CGE) is rich in flavonoids, phenolic acids and phytosterols.•CGE reduced pro-inflammatory cytokines levels, modulated tight junction proteins and apoptotic damage of the colonic tissue.•CGE inhibited the activation of LPS/TLR4/NF-ҡB and NLRP3 signaling pathways.•CGE in a dose of 200 mg/kg was more efficacious in attenuating UC when compared to sulphasalazine “Reference drug”.
Three metal complexes of Gd (III), Pr (III) and Ru (III) metal ions with Schiff base ligand (H2L) (prepared through l:2 condensation of dibenzoyl methane and anthranilic acid) were prepared and ...characterized using various physio‐chemical methods like: elemental analyses, IR, mass spectrometry, magnetic moment, 1H NMR, SEM and TG/DTG thermal analysis. The analytical and spectroscopic tools showed that the complexes had composition of ML type with octahedral geometry. The mass spectra gave the possible molecular ion peaks of the Schiff base ligand and three metal chelates. The 1H NMR data supported the IR finding that the ligand coordinated to the metal ions via carboxylate proton displacement. Thermal analysis (TG/DTG) was utilized to differentiate between coordinated and hydrated water molecules. The Schiff base (H2L) and its metal complexes have been screened for their antibacterial activity against Gram (+) bacteria (Streptococcus aureus and Bacillis subtilis), Gram (−) bacteria (Salmonella typhimurium and Escherichia coli) and two fungi (Aspergillus fumigatu and Candida albicans) organisms by agar diffusion method. The anticancer activity was screened against human breast cancer cell line (MCF‐7). The H2L ligand and its metal chelates were docked using MOE 2008 software with crystal structure of Gram (+) bacteria: Staphylococcus aureus (PDB ID: 3Q8U) and Gram (−) bacteria: Salmonella typhimurium (PDB ID: lDZR) to identify the binding orientation or conformation of the complex in the active site of the protein.
• Schiff base ligand, H2L and its three new lanthanide metal complexes were prepared and characterized using different tools.• Molecular docking studies of the free Schiff base ligand and Ru (III) complex with receptors of different proteins reveal Ru (III) complex show highest binding ability and the minimum biding energy.• The results of anticancer assay of ligand and the complexes suggested the Ru (III) complex exhibited the lowest IC50 was more effective against breast cancer cell line.
