The clinical and laboratory criteria for hemophagocytic lymphohistiocytosis should be taken into account during the juvenile myelomonocytic leukemia diagnosis, specifically in CBL syndrome, to reveal ...the presence of primary rather than secondary associated hemophagocytosis.
To compare the left ventricular function and the ventricular synchrony in patients with Chagas disease in latency stage respect to a control group.
We analyze a prospective, comparative, transversal ...and non randomized study of the left ventricular function (LVF) and the ventricular contraction synchronicity (VCS) in 36 subjects with positive serology for Chagas disease (18 males and 18 females), with mean of 15 +/- 5-years-old. The findings were compared with respect to 23 control volunteers (11 males and 12 females) with mean of 28 +/- 5-years-old. LVF and VCS were evaluated using equilibrium radionuclide angiography images (ERNA). The comparison of both Chagas and control populations was carried out by t Student test for independent samples, considering a statistically significant value of p < 0.05.
The parameters of the ventricular function and the ventricular synchronicity in subjects with positive serology for Chagas disease were not statistically different with respect to the parameters of the control group. However, although they have a homogeneous contraction, the mean time of contraction for the right and the left ventricle is statistically smaller with respect to the control group.
In clinically incipient stages of Chagas disease we do not found abnormalities in the ventricular function and the ventricular synchronicity. It's necessary to consider the follow up of the studied populations using indices for the identification of abnormalities of the autonomic nervous system.
México Francia Boadella, Montserrat Galí; Cházaro, Laura; Christlieb, Federico Fernández ...
1993
eBook, Book
Open access
"¿Ganaríamos los mexicanos, si la razón o el capricho nos condujese a un rápido desafrancesamiento?", fue la pregunta que en 1867, al triunfo de los juaristas, formuló Ignacio Ramírez a los ...intelectuales que desataron un revuelo antigalo. El Nigromante probó entonces la existencia de numerosas influencias galas -conservadoras, monárquicas, liberales, republicanas- en la historia y la cultura mexicanas. Y reveló así el arraigo de diversas sensibilidades afrancesadas. Unidos en un proyecto colectivo, investigadores de varias instituciones del país exploramos ese hallazgo. En cinco ejes analíticos este libro ilustra la construcción de sensibilidades afrancesadas en los siglos XIX y XX. El primer eje se consagra al estudio de los inmigrantes galos, sus miradas, prácticas e intereses, y prueba la articulación de un milieu afrancesado. El segundo examina la influencia en las urbes y, a través de ejemplos de comercios galos en Puebla y la ciudad de México "el París de América"-, revela su influencia en la creación de una cultura urbana moderna. La participación de galos en la educación de las élites -preceptores en Chihuahua, órdenes religiosas en el país- y en exploraciones científicas son objeto del tercer eje analítico. Mientras que las huellas del modelo médico francés en prácticas clínicas, terapias y en ideas higienistas se tratan en el cuatro eje. El libro cierra con estudios que revelan el afrancesamiento de gustos, estilos y posturas literarias, así como la influencia de representaciones de viajeros galos. Basado en fuentes novedosas, este libro -segundo fruto de un proyecto académico- confirma la pertinencia de una problemática -la construcción de sensibilidades-y la utilidad de una perspectiva -la mundialización-para el estudio de la presencia extranjera en México.
Urapidil, a postsynaptic alpha 1 -adrenergic antagonist, has been reported to improve intraoperative hemodynamic stability, although it has never been used to prevent the hemodynamic response of ...electroconvulsive therapy (ECT). This study was designed to evaluate the clinical effectiveness of urapidil, as an alternative to labetalol, in preventing the hemodynamic response of ECT. Twenty-seven patients undergoing a series of six consecutive ECT treatments were studied. Each patient received all three pretreatments twice: no drug, labetalol 0.2 mg/kg, or urapidil 25 mg. Systolic, diastolic, and mean blood pressure and heart rate (HR) were recorded during the awake state, after anesthesia induction, and 1, 2, 5, 10 and 30 minutes after electroencephalographic (EEG) seizure ended. The duration of the EEG convulsion was also recorded. After induction, the HR increased for no drug and urapidil pretreatments, whereas it decreased when labetalol was given. Labetalol and urapidil attenuated the peak increase of blood pressure and returned it to earlier baseline values. There were no differences in the duration of EEG convulsion between the three pretreatments. Urapidil seems to be a good alternative to labetalol for attenuating the hypertensive response to ECT in cases where there is a contraindication to beta-antagonists.
Lynch syndrome (LS) is an autosomal dominant inherited cancer syndrome characterized by early onset cancers of the colorectum, endometrium and other tumours. A significant proportion of DNA variants ...in LS patients are unclassified. Reports on the pathogenicity of the c.1852_1853AA>GC (p.Lys618Ala) variant of the MLH1 gene are conflicting. In this study, we provide new evidence indicating that this variant has no significant implications for LS. The following approach was used to assess the clinical significance of the p.Lys618Ala variant: frequency in a control population, case-control comparison, co-occurrence of the p.Lys618Ala variant with a pathogenic mutation, co-segregation with the disease and microsatellite instability in tumours from carriers of the variant. We genotyped p.Lys618Ala in 1034 individuals (373 sporadic colorectal cancer CRC patients, 250 index subjects from families suspected of having LS revised Bethesda guidelines and 411 controls). Three well-characterized LS families that fulfilled the Amsterdam II Criteria and consisted of members with the p.Lys618Ala variant were included to assess co-occurrence and co-segregation. A subset of colorectal tumour DNA samples from 17 patients carrying the p.Lys618Ala variant was screened for microsatellite instability using five mononucleotide markers. Twenty-seven individuals were heterozygous for the p.Lys618Ala variant; nine had sporadic CRC (2.41%), seven were suspected of having hereditary CRC (2.8%) and 11 were controls (2.68%). There were no significant associations in the case-control and case-case studies. The p.Lys618Ala variant was co-existent with pathogenic mutations in two unrelated LS families. In one family, the allele distribution of the pathogenic and unclassified variant was in trans, in the other family the pathogenic variant was detected in the MSH6 gene and only the deleterious variant co-segregated with the disease in both families. Only two positive cases of microsatellite instability (2/17, 11.8%) were detected in tumours from p.Lys618Ala carriers, indicating that this variant does not play a role in functional inactivation of MLH1 in CRC patients. The p.Lys618Ala variant should be considered a neutral variant for LS. These findings have implications for the clinical management of CRC probands and their relatives.
Studies on family care giving within hospitals are not frequent. This situation contributes to make these people underconsidered by the health system. The aim of this study is to analyze the profile ...of family caregivers, the needs they attend and how they are considered by the health professionals in a traumatology and rehabilitation hospital.
Methodology: Descriptive and cross-sectional study, which included one hundred interviews performed between September and October 2002 in traumatology, maxillofacial surgery, neurology, and neurosurgery wards.
Results: The family caregiver profile corresponds to a female with a median age of 48 years who spends a mean of 20 hours per day in the hospital. Surveillance and company were the main contributions to the patient care according to caregivers’ answers.
Conclusions: Wives or mothers become the main family caregivers. Their contribution implies monetary and time costs as well as physic, psychological and social impact. The care offered by the family caregivers is scarcely supported by professional help. Increasing the professionals’ recognition of the family caregivers is a social requirement.
Los estudios de cuidadores familiares realizados dentro de las instituciones hospitalarias son escasos. Esta situación determina su invisibilidad y su no reconocimiento por parte del sistema institucional sanitario. Los objetivos de este estudio son estudiar el perfil de los cuidadores familiares en el Hospital Universitario de Traumatología y Rehabilitación de Granada, conocer el tipo de necesidades que cubren, las repercusiones y su valoración por los profesionales sanitarios.
Metodología:
Investigación de tipo descriptivo y transversal, mediante 100 encuestas realizadas entre septiembre y octubre de 2002, en las plantas de traumatología, maxilofacial, neurología, neurocirugía. Se efectuó un análisis descriptivo y bivariante a través de tablas de contingencia y diferencias de media. Las variables fueron edad, sexo, ingresos económicos, vivienda conjunta, trabajo y localidad del cuidador.
Resultados: El perfil del cuidador informal corresponde a una mujer, con una media de edad de 48 años y que permanece 20 horas de media al día en el hospital. Las necesidades que el cuidador percibe como más importantes para el paciente estuvieron relacionadas con la compañía y la vigilancia.
Conclusiones: El perfil del cuidador familiar es de cónyuges o madres. La realización de las tareas de cuidador supone un gasto de tiempo, dinero, desgaste físico, psicológico y social. Parte de las necesidades del paciente son cubiertas por los cuidadores informales, los cuales reciben poca información y asesoramiento del personal de enfermería sobre cómo realizarlas. Ampliar por tanto la mirada enfermera es una exigencia y demanda de nuestra sociedad.
Blood drainage of the graft into the recipient portal vein reestablishes the physiological venous outflow after small bowel transplantation (SBT). However, although this approach is likely beneficial ...for the host, it may be technically more demanding making portocaval venous drainage the preferred arrangement during human SBT. The aim of this study was to examine in a syngeneic model of SBT the possible benefits of portoportal anastomosis (PPA) vs portocaval anastomosis (PCA) in terms of body and organ weights and bacterial translocation.
Syngeneic SBT was carried out in 25 Brown-Norway male rats weighing 249 ± 17.5 g using either PPA (n = 13) or PCA (n = 12). Half the animals in each group were killed, respectively, on postoperative day 2 or 7. Liver, spleen, and lungs were weighed and under sterile conditions the regional lymph nodes were excised. The nodes and venous samples from the cava and portal veins were cultured for aerobes and anaerobes. Bacterial components were detected in blood by polymerase chain reaction. The findings in both groups were compared by
χ
2 or Mann-Whitney
U tests.
Mean postoperative body weight change was −3.6% ± 1.5% in PPA and −6.0% ± 1.2% in PCA animals (ns) on day 2 and −6.5% ± 2.6% and −8.0% ± 5.0% (ns) on day 7. Liver, spleen, and lung weights were not significantly different between both groups on either end point. Gram-negative enteric bacteria were found in 3 of 7 PCA animals and 2 of 6 PPA animals at day 2 (ns) and in 1 of 6 and 4 of 6 on day 7 (ns). Aerobic gram-positive bacteria were found in 1 of 7 and 1 of 6 (ns), 3 of 6 and 3 of 6 (ns), respectively, in the 4 groups. Most positive cultures corresponded to portal blood and lymph node samples. There were no anaerobic growths.
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No body or organ weight change suggesting significant functional advantages of one technical alternative over the other could be demonstrated.
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Bacterial translocation in the absence of rejection was frequent after SBT independently of the variety of venous outflow used. No difference in bacterial translocation between both anastomosis could be demonstrated.
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Orthotopic venous drainage did not seem to be advantageous in the present experimental setting.
We report the case of a 3-year-old girl, the only child of a nonconsanguineous couple without relevant antecedents, who was born with hydranencephalic-hydrocephalic syndrome diagnosed by ...ultrasonography at gestation week 28, and who was treated during the neonatal period by implantation of a ventriculoperitoneal shunt. She showed severe mental retardation, and died at age 4 years following an acute respiratory infection. Due to persistently high lactic acid levels in blood, muscle and skin biopsies were taken. Analysis of muscle biopsies revealed microscopic and ultrastructural alterations typical of mitochondrial disorders, and low levels of complexes III and IV of the mitochondrial respiratory chain. The enzymes of the pyruvate dehydrogenase complex showed normal activities in cultured skin fibroblasts. These findings raise the possibility that at least some cases of congenital hydranencephalic-hydrocephalic syndrome may be due to alterations in the mitochondrial respiratory chain. (J Child
Neurol 1999; 14: 131-135).
Phosphorylation of maize RAB-17 protein by casein kinase 2 Plana, M. (Universitat Autonoma de Barcelona, Barcelona, Spain); Itarte, E; Eritja, R ...
Journal of biological chemistry/The Journal of biological chemistry,
11/1991, Volume:
266, Issue:
33
Journal Article
Peer reviewed
Open access
The maize gene RAB-17, which is responsive to abscisic acid, encodes a basic glycine-rich protein containing, in the middle part of its sequence, a cluster of 8 serine residues followed by a putative ...casein kinase 2-type substrate consensus sequence. This protein was found to be highly phosphorylated in vivo. Here, we show that RAB-17 protein is a real substrate for casein kinase 2. RAB-17 protein is phosphorylated in vitro by casein kinase 2 isolated from rat liver cytosol and from maize embryos. A maximum of 4 mol of phosphate were incorporated per mol of RAB-17 protein following incubation with casein kinase 2. Phosphopeptide mapping experiments show that the peptide phosphorylated by casein kinase 2 in vitro is identical to that derived from the protein phosphorylated in vivo. Purification by high performance liquid chromatography and partial sequencing of the phosphopeptide indicate that it corresponds to the region of the protein (residues 56-89) containing the cluster of serine residues. Our results indicate that RAB-17 is phosphorylated by casein kinase 2 or a kinase with a similar specificity and that phosphorylation takes place in the serine cluster region of the protein both in vitro and in vivo
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