Tomato is an agricultural product of great economic importance because it is one of the most consumed vegetables in the world. The most crucial chemical element for the growth and development of ...tomato is nitrogen (N). However, incorrect nitrogen usage can alter the quality of tomato fruit, rendering it undesirable to customers. Therefore, the goal of the current study is to investigate the early detection of excess nitrogen application in the leaves of the Royal tomato variety using a non-destructive hyperspectral imaging system. Hyperspectral information in the leaf images at different wavelengths of 400–1100 nm was studied; they were taken from different treatments with normal nitrogen application (A), and at the first (B), second (C) and third (D) day after the application of excess nitrogen. We investigated the performance of nine machine learning classifiers, including two classic supervised classifiers, i.e., linear discriminant analysis (LDA) and support vector machines (SVMs), three hybrid artificial neural network classifiers, namely, hybrid artificial neural networks and independent component analysis (ANN-ICA), harmony search (ANN-HS) and bees algorithm (ANN-BA) and four classifiers based on deep learning algorithms by convolutional neural networks (CNNs). The results showed that the best classifier was a CNN method, with a correct classification rate (CCR) of 91.6%, compared with an average of 85.5%, 68.5%, 90.8%, 88.8% and 89.2% for LDA, SVM, ANN-ICA, ANN-HS and ANN-BA, respectively. This shows that modern CNN methods should be preferred for spectral analysis over other classical techniques. These CNN architectures can be used in remote sensing for the precise detection of the excessive use of nitrogen fertilizers in large extensions.
This study focuses on the spectrochemical estimation of pH and titratable acidity (TA) of apples of Fuji variety at different stages of ripening. A novel approach is proposed for near-infrared (NIR) ...spectral analysis using hybrid machine learning methods that combine artificial neural networks (ANN) and metaheuristic algorithms. One hundred twenty samples were collected at three ripening stages and spectral data within two bands of NIR were extracted from each sample to predict the acidity properties. Alternatively, the 4 most effective wavelengths were also selected using a hybrid of ANN and the cultural algorithm. The experimental results prove that the models using spectral bands and the 4 most effective wavelengths are comparable, with a correlation coefficient, R, of 0.926 for the prediction of pH and 0.925 for TA using spectral bands, while for the second approach the R obtained were 0.924 and 0.920 for pH and TA, respectively. The models could not accurately predict extremely high or low pH and TA values, due to the clusters that formed after regression. However, for a classification problem in low/high acidity, both approaches were able to achieve a high accuracy of 100% for pH and 99.2% for TA.
NAD+ has emerged as a crucial element in both bioenergetic and signaling pathways since it acts as a key regulator of cellular and organismal homeostasis. Among the enzymes involved in its recycling, ...nicotinamide mononucleotide (NMN) deamidase is one of the key players in the bacterial pyridine nucleotide cycle, where it catalyzes the conversion of NMN into nicotinic acid mononucleotide (NaMN), which is later converted to NAD+ in the Preiss-Handler pathway. The biochemical characteristics of bacterial NMN deamidases have been poorly studied, although they have been investigated in some firmicutes, gamma-proteobacteria and actinobacteria. In this study, we present the first characterization of an NMN deamidase from an alphaproteobacterium, Agrobacterium tumefaciens (AtCinA). The enzyme was active over a broad pH range, with an optimum at pH 7.5. Moreover, the enzyme was quite stable at neutral pH, maintaining 55% of its activity after 14 days. Surprisingly, AtCinA showed the highest optimal (80°C) and melting (85°C) temperatures described for an NMN deamidase. The above described characteristics, together with its high catalytic efficiency, make AtCinA a promising biocatalyst for the production of pure NaMN. In addition, six mutants (C32A, S48A, Y58F, Y58A, T105A and R145A) were designed to study their involvement in substrate binding, and two (S31A and K63A) to determine their contribution to the catalysis. However, only four mutants (C32A, S48A Y58F and T105A) showed activity, although with reduced catalytic efficiency. These results, combined with a thermal and structural analysis, reinforce the Ser/Lys catalytic dyad mechanism as the most plausible among those proposed.
Color segmentation is one of the most thoroughly studied problems in agricultural applications of remote image capture systems, since it is the key step in several different tasks, such as crop ...harvesting, site specific spraying, and targeted disease control under natural light. This paper studies and compares five methods to segment plum fruit images under ambient conditions at 12 different light intensities, and an ensemble method combining them. In these methods, several color features in different color spaces are first extracted for each pixel, and then the most effective features are selected using a hybrid approach of artificial neural networks and the cultural algorithm (ANN-CA). The features selected among the 38 defined channels were the b* channel of L*a*b*, and the color purity index, C*, from L*C*h. Next, fruit/background segmentation is performed using five classifiers: artificial neural network-imperialist competitive algorithm (ANN-ICA); hybrid artificial neural network-harmony search (ANN-HS); support vector machines (SVM); k nearest neighbors (kNN); and linear discriminant analysis (LDA). In the ensemble method, the final class for each pixel is determined using the majority voting method. The experiments showed that the correct classification rate for the majority voting method excluding LDA was 98.59%, outperforming the results of the constituent methods.
The area of remote sensing techniques in agriculture has reached a significant degree of development and maturity, with numerous journals, conferences, and organizations specialized in it. Moreover, ...many review papers are available in the literature. The present work describes a literature review that adopts the form of a systematic mapping study, following a formal methodology. Eight mapping questions were defined, analyzing the main types of research, techniques, platforms, topics, and spectral information. A predefined search string was applied in the Scopus database, obtaining 1590 candidate papers. Afterwards, the most relevant 106 papers were selected, considering those with more than six citations per year. These are analyzed in more detail, answering the mapping questions for each paper. In this way, the current trends and new opportunities are discovered. As a result, increasing interest in the area has been observed since 2000; the most frequently addressed problems are those related to parameter estimation, growth vigor, and water usage, using classification techniques, that are mostly applied on RGB and hyperspectral images, captured from drones and satellites. A general recommendation that emerges from this study is to build on existing resources, such as agricultural image datasets, public satellite imagery, and deep learning toolkits.
To achieve healthy and optimal yields of agricultural products, the principles of nutrition must be observed and appropriate fertilizers must be applied. Nutritional deficiencies or overabundance ...reduce the quality and yield of the products. Thus, their early detection prevents physiological disorders and associated diseases. Most research efforts have focused on spectroscopy, which extracts only spectral data from a single point of the product. The present study aims to detect early excess nitrogen in cucumber plants by using a new hyperspectral imaging technique based on a hybrid of artificial neural networks and the imperialist competitive algorithm (ANN-ICA), which can provide spectral and spatial information on the leaves at the same time. First, cucumber seeds were planted in 18 pots. The same inputs were applied to all the pots until the plants grew; after that, 30% excess nitrogen was applied to nine pots with irrigation water, while it remained constant in the other nine pots. Each day, six leaves were collected from each pot, and their images were captured using a hyperspectral camera (in the range of 400–1100 nm). The wavelengths of 715, 783 and 821 nm were determined as the most effective for early detection of excess nitrogen using a hybrid of artificial neural networks and the artificial bee colony algorithm (ANN-ABC). The parameter of days of treatment was classified using ANN-ICA. The performance of the classifier was evaluated using different criteria, namely recall, accuracy, specificity, precision and the F-measure. The results indicate that the differences between different days were statistically significant. This means that the hyperspectral imaging technique was able to detect plants with excess nitrogen in the near-infrared range (NIR), with a correct classification rate of 96.11%.
There are about 90 different varieties of chickpeas around the world. In Iran, where this study takes place, there are five species that are the most popular (Adel, Arman, Azad, Bevanij and Hashem), ...with different properties and prices. However, distinguishing them manually is difficult because they have very similar morphological characteristics. In this research, two different computer vision methods for the classification of the variety of chickpeas are proposed and compared. The images were captured with an industrial camera in Kermanshah, Iran. The first method is based on color and texture features extraction, followed by a selection of the most effective features, and classification with a hybrid of artificial neural networks and particle swarm optimization (ANN-PSO). The second method is not based on an explicit extraction of features; instead, image patches (RGB pixel values) are directly used as input for a three-layered backpropagation ANN. The first method achieved a correct classification rate (CCR) of 97.0%, while the second approach achieved a CCR of 99.3%. These results prove that visual classification of fruit varieties in agriculture can be done in a very precise way using a suitable method. Although both techniques are feasible, the second method is generic and more easily applicable to other types of crops, since it is not based on a set of given features.
One of the most important applications of remote imaging systems in agriculture, with the greatest impact on global sustainability, is the determination of optimal crop irrigation. The methodology ...proposed by the Food and Agriculture Organization (FAO) is based on estimating crop evapotranspiration (ETc), which is done by computing the reference crop evapotranspiration (ETo) multiplied by a crop coefficient (Kc). Some previous works proposed methods to compute Kc using remote crop images. The present research aims at complementing these systems, estimating ETo with the use of soil moisture sensors. A crop of kikuyu grass (Pennisetum clandestinum) was used as the reference crop. Four frequency-domain reflectometry sensors were installed, gathering moisture information during the study period from May 2015 to September 2016. Different machine learning regression algorithms were analyzed for the estimation of ETo using moisture and climatic data. The values were compared with respect to the ETo computed in an agroclimatic station using the Penman–Monteith method. The best method was the randomizable filtered classifier technique, based on the K* algorithm. This model achieved a correlation coefficient, R, of 0.9936, with a root-mean-squared error of 0.183 mm/day and 6.52% mean relative error; the second-best model used artificial neural networks, with an R of 0.9470 and 11% relative error. Thus, this new methodology allows obtaining accurate and cost-efficient prediction models for ETo, as well as for the water balance of the crops.
The estimation of the ripening state in orchards helps improve post-harvest processes. Picking fruits based on their stage of maturity can reduce the cost of storage and increase market outcomes. ...Moreover, aerial images and the estimated ripeness can be used as indicators for detecting water stress and determining the water applied during irrigation. Additionally, they can also be related to the crop coefficient (Kc) of seasonal water needs. The purpose of this research is to develop a new computer vision algorithm to detect the existing fruits in aerial images of an apple cultivar (of Red Delicious variety) and estimate their ripeness stage among four possible classes: unripe, half-ripe, ripe, and overripe. The proposed method is based on a combination of the most effective color features and a classifier based on artificial neural networks optimized with genetic algorithms. The obtained results indicate an average classification accuracy of 97.88%, over a dataset of 8390 images and 27,687 apples, and values of the area under the ROC (receiver operating characteristic) curve near or above 0.99 for all classes. We believe this is a remarkable performance that allows a proper non-intrusive estimation of ripening that will help to improve harvesting strategies.
Due to the limitations of drones and satellites to obtain aerial images of the crops in real time, the time to flight delay, the problems caused by adverse weather conditions and other issues, the ...use of fixed cameras placed on the regions of interest is essential to get closer, periodic and on-demand images. Water management in agriculture is one of the most important applications of these images. Top view images of a crop can be processed for determining the percentage of green cover (PGC), and 2D images from different viewing angles can be applied for obtaining 3D models of the crops. In both cases, the obtained data can be managed for calculating several parameters such as crop evapotranspiration, water demand, detection of water deficit and indicators about solute transport of fertilizers in the plant. For this purpose, a remote image capture system has been developed for an application in lettuce crops. The system consists of several capture nodes and a local processing base station which includes image processing algorithms to obtain key features for decision-making in irrigation and harvesting strategies. Placing multiple image capture nodes allows obtaining different observation zones that are representative of the entire crop. The nodes have been designed to have autonomous power supply and wireless connection with the base station. This station carries out irrigation and harvesting decisions using the results of the processing of the images captured by the nodes and the information of other local sensors. The wireless connection is made using the ZigBee communication architecture, supported by XBee hardware. The two main benefits of this choice are its low energy consumption and the long range of the connection.
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