Molecular analysis of Limonium genus through RAPD markers [Random Amplified Polymorphic DNA] Bruna, S. (Consiglio per la Ricerca e la Sperimentazione in Agricoltura (CRA) - Istituto Sperimentale per la Floricoltura, Sanremo, Imperia (Italy)); Burchi, G. (Consiglio per la Ricerca e la Sperimentazione in Agricoltura (CRA) - Istituto Sperimentale per la Floricoltura, Sanremo, Imperia (Italy)); Benedetti, L. De (Consiglio per la Ricerca e la Sperimentazione in Agricoltura (CRA) - Istituto Sperimentale per la Floricoltura, Sanremo, Imperia (Italy)) ...
Agricoltura Mediterranea (Italy),
(2005), Volume:
135, Issue:
1
Journal Article
RAPD analysis was used to determine the genetic similarity in wild species and cultivated varieties of Limonium genus and for verification of hybridity in progenies of interspecific crosses. Eleven ...wild species and ten commercial varieties were tested with ten selected RAPD primers. A total of 254 bands were scored and used for calculating genetic distances among entries. Two cultivars were grouped together with L. sinensis. The species L. bellidifolium, L. otolepis and L. caspia were clustered together. The largest cluster grouped five varieties and L. latifolium. The remaining three cultivars, belonging to the Altaica group, resulted related to L. gmelinii. The wild species L. tataricum, L. perezii, L. sinuatum and L. longifolium were clearly separated from all of the other genotypes. Seven putative interspecific hybrids and their parents were characterised by means of nine selected RAPD primers. The putative parentage was confirmed for only two hybrids; the remaining five genotypes, on the basis of RAPD patterns, were identified as apomictic and/or self-pollinated. Our results demonstrated that RAPD analysis is a useful tool for the molecular characterization of Limonium genotypes. This simple technique may enable ornamental plant breeders to improve the efficiency of their breeding programs
E' stata utilizzata l'analisi RAPD per determinare la somiglianza genetica in specie spontanee e varietà coltivate del genere Limonium e per la verifica dell'ibridismo nelle progenie di incroci interspecifici. Sono state saggiate con dieci marcatori RAPD selezionati undici specie spontanee e dieci varietà commerciali. Sono state riscontrate 250 bande in totale, utilizzate per calcolare le distanze genetiche fra le accessioni. Due cultivar erano raggruppate con L. sinensis. Le specie L. bellidifolium, L. optolepis e L. caspia erano raggruppate assieme. Il cluster più ampio comprendeva cinque varietà e L. latifolium. Le tre cultivar rimanenti, appartenenti al gruppo Altaica, sono risultate correlate con L. gmelinii. Le specie spontanee L. tataricum, L. perezii, L. sinuatum e L. longifolium erano separate nettamente da tutti gli altri genotipi. Sette ibridi interspecifici putativi e i loro parentali sono stati caratterizzati per mezzo di nove primer RAPD selezionati. La loro ascendenza putativa è stata confermata solo per due ibridi; i cinque rimanenti genotipi, in base ai dati RAPD, sono stati identificati come apomittici e/o derivanti da autoimpollinazione. I nostri risultati hanno dimostrato che l'analisi RAPD è uno strumento utile per la caratterizzazione molecolare dei genotipi di Limonium. Questa tecnica semplice può consentire di migliorare l'efficienza dei programmi di miglioramento genetico.
It is generally accepted that Lp(a) is an independent risk factor of cardiovascular diseases. Since the apolipoprotein component of Lp(a) shows some homologies to plasminogen, it is, however, unclear ...as to whether the pathological effect is due to the role played by the lipoprotein in lipid metabolism or in the fibrinolytic system. We compared two groups of patients with myocardial infarction, with and without angiographically documented coronary artery disease. In the latter group, imbalances in the clotting system are very likely, while members of the former group may also display disturbances of lipid metabolism. The results show that the two groups display differences in lipid metabolism, whereas they have similar patterns of thrombogenicity indices and Lp(a) values. This study seems to support the hypothesis that Lp(a) does play a role in the fibrinolytic system, since even those myocardial infarctions without obstructive coronary artery disease have a high frequency of Lp(a) concentrations above 300 mg/l, i.e. similar to the situation found in the myocardial infarctions with angiographically documented coronary artery disease. Whether the high Lp(a) concentrations in the two groups are related to an impaired fibrinolysis will be the subject of further investigation.
Severe asthma (SA) is a heterogeneous disease with multiple molecular mechanisms. Gene expression studies of bronchial epithelial cells in individuals with asthma have provided biological insight and ...underscored possible mechanistic differences between individuals.
Identify networks of genes reflective of underlying biological processes that define SA.
Airway epithelial cell gene expression from 155 subjects with asthma and healthy control subjects in the Severe Asthma Research Program was analyzed by weighted gene coexpression network analysis to identify gene networks and profiles associated with SA and its specific characteristics (i.e., pulmonary function tests, quality of life scores, urgent healthcare use, and steroid use), which potentially identified underlying biological processes. A linear model analysis confirmed these findings while adjusting for potential confounders.
Weighted gene coexpression network analysis constructed 64 gene network modules, including modules corresponding to T1 and T2 inflammation, neuronal function, cilia, epithelial growth, and repair mechanisms. Although no network selectively identified SA, genes in modules linked to epithelial growth and repair and neuronal function were markedly decreased in SA. Several hub genes of the epithelial growth and repair module were found located at the 17q12-21 locus, near a well-known asthma susceptibility locus. T2 genes increased with severity in those treated with corticosteroids but were also elevated in untreated, mild-to-moderate disease compared with healthy control subjects. T1 inflammation, especially when associated with increased T2 gene expression, was elevated in a subgroup of younger patients with SA.
In this hypothesis-generating analysis, gene expression networks in relation to asthma severity provided potentially new insight into biological mechanisms associated with the development of SA and its phenotypes.
c. 1r Modena, Giacomo <n. 1766>
09/1802
Web Resource
1802-09-03 (c. 1r) · cart. · 1 bifolio · cc. 2 · mm 226×190 (c. 1). Possessore: Obizzi, Tommaso <1750-1803> (Biondi, 27-35; Favaretto, 243-248). c. 1r Lettera, originale, con firma autografa (Bianche ...cc. 1v-2r). Mittente (autografo): Modena, Giacomo <n. 1766> (Regli, 334-337). Destinatario: Obizzi, Tommaso <1750-1803> (Biondi, 27-35; Favaretto, 243-248). Padova, 1802-09-03 (c. 1r). Osservazioni: indicazione del destinatario a c. 2v. Fonti: F. Regli, Dizionario biografico dei più celebri poeti ed artisti melodrammatici, tragici e comici, maestri, concertisti, coreografi, mimi, ballerini, scenografi, giornalisti, impresarii, ecc. ecc. che fiorirono in Italia dal 1800 al 1860, Torino 1860. I. Favaretto, Arte antica e cultura antiquaria nelle collezioni venete al tempo della Serenissima, Roma 1990. G. Biondi, Tommaso Obizzi e gli ultimi Estensi: momenti di un singolare rapporto, in "Gli Estensi e il Cataio: aspetti del collezionismo tra Sette e Ottocento", a cura di E. Corradini, Modena 2007, 27-35. Fondo: C.A. Lingue: Italiano. Soggetto: Epistolari. Catalogazione: Daniela Camanzi. Data creazione scheda: 25 ottobre 2011. Data ultima modifica: 23 novembre 2011.
Although asthma is recognized as a heterogeneous disease associated with clinical phenotypes, the molecular basis of these phenotypes remains poorly understood. Although genomic studies have ...successfully broadened our understanding in diseases such as cancer, they have not been widely used in asthma studies.
To link gene expression patterns to clinical asthma phenotypes.
We used a microarray platform to analyze bronchial airway epithelial cell gene expression in relation to the asthma biomarker fractional exhaled nitric oxide (FeNO) in 155 subjects with asthma and healthy control subjects from the Severe Asthma Research Program (SARP).
We first identified a diverse set of 549 genes whose expression correlated with FeNO. We used k-means to cluster the patient samples according to the expression of these genes, identifying five asthma clusters/phenotypes with distinct clinical, physiological, cellular, and gene transcription characteristics-termed "subject clusters" (SCs). To then investigate differences in gene expression between SCs, a total of 1,384 genes were identified that highly differentiated the SCs at an unadjusted P value < 10(-6). Hierarchical clustering of these 1,384 genes identified nine gene clusters or "biclusters," whose coexpression suggested biological characteristics unique to each SC. Although genes related to type 2 inflammation were present, novel pathways, including those related to neuronal function, WNT pathways, and actin cytoskeleton, were noted.
These findings show that bronchial epithelial cell gene expression, as related to the asthma biomarker FeNO, can identify distinct asthma phenotypes, while also suggesting the presence of underlying novel gene pathways relevant to these phenotypes.
Mass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a ...low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10−20 g/mL to 10−14 g/mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C–H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources.
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•Low-cost immunosensors to detect the spike protein of SARS-CoV-2 virus.•Limit of detection of 0.179 fg/mL for SARS-CoV-2 spike protein using electrical impedance spectroscopy.•Tests can be made with portable instruments for point-of-care diagnostics.•The immunosensors were selective for the spike protein, without interference from other biomarkers.
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