Hepatic cancer is among the most recurrently detected malignancies worldwide and one of the main contributors to cancer-associated mortality. With few available therapeutic choices, there is an ...instant necessity to explore suitable options. In this aspect, Nanotechnology has been employed to explore prospective chemotherapeutic approaches, especially for cancer treatment. Nanotechnology is concerned with the biological and physical properties of nanoparticles in the therapeutic use of drugs. In the current work, formulation, and characterization of α-Fe2O3–Sodium Alginate-Eugenol nanocomposites (FSE NCs) using several approaches like SEM and TEM, UV–visible, FTIR, and PL spectroscopy, XRD, EDAX, and DLS studies have been performed. With an average size of 50 nm, the rhombohedral structure of NCs was identified. Further, their anticancer activity against Hep3B liver cancer cell lines has been performed by cell viability, dual staining, DCFH-DA, Annexin-V/-FITC/PI, cell cycle analysis methods, and PI3K/Akt/mTOR signaling proteins were studied to assess the anticancer effects of the NCs in Hep3B cells. Also, anti-cancer activity on animal modeling in-vivo using zebra fishes to hematological parameters, liver enzymes, and histopathology study effectiveness was noticed. Moreover, the NCs reduced the viability, elevated the ROS accumulation, diminished the membrane integrity, reduced the antioxidants, blocked the cell cycle, and triggered the PI3K/Akt/mTOR signaling axis that eventually resulted in cell death. As a result, FSE NCs possess huge potential for use as a possible anticancer candidate.
Currently, new advancements in the area of nanotechnology opened up new prospects in the field of medicine that could provide us with a solution for numerous medical complications. Although a several ...varieties of nanoparticles is being explored to be used as nanomedicines, cerium oxide nanoparticles (CeO2 NPs) are the most attractive due to their biocompatibility and their switchable oxidation state (+3 and +4) or in other words the ability to act as prooxidant and antioxidant depending on the pH condition. Green synthesis of nanoparticles is preferred to make it more economical, eco-friendly, and less toxic. The aim of our study here is to formulate the CeO2 NPs (CeO2 NPs) using Morinda citrifolia (Noni) leaf extract and study its optical, structural, antibacterial, and anticancer abilities. Their optical and structural characterization was accomplished by employing X-ray diffractography (XRD), TEM, EDAX, FTIR, UV-vis, and photoluminescence assays. Our CeO2 NPs expressed strong antibacterial effects against Gram-positive S. aureus and S. pneumonia in addition to Gram-negative E. coli and K. pneumonia when compared with amoxicillin. The anticancer properties of the green synthesized CeO2 NPs against human acute lymphoblastic leukemia (ALL) MOLT-4 cells were further explored by the meticulous study of their ability to diminish cancer cell viability (cytotoxicity), accelerate apoptosis, escalate intracellular reactive oxygen species (ROS) accumulation, decline the mitochondria membrane potential (MMP) level, modify the cell adhesion, and shoot up the activation of proapoptotic markers, caspase−3, −8, and −9, in the tumor cells. Altogether, the outcomes demonstrated that our green synthesized CeO2 NPs are an excellent candidate for alternative cancer therapy.
In this study, we evaluated the antiproliferative and apoptotic properties of Pluronic-F127-containing manganese oxide nanoparticles (PF-127-coated Mn
NPs) derived from the leaf extract of
(GU) on ...breast adenocarcinoma, MCF7, and MDA-MB-231 cell lines. The leaf extract of GU contains bioactive molecules that act as a reducing or capping agent to form Mn
NPs. Various analytical techniques were used to characterize the physiochemical properties of PF-127-coated Mn
NPs, including spectroscopy (ultralight-Vis, Fourier transform infrared, photoluminescence), electron microscopy (field emission scanning electron microscopy and transmission electron microscopy), X-ray diffraction (XRD), electron diffracted X-ray spectroscopy (EDAX), and dynamic light scattering. The average crystallite size of Mn
NPs was estimated to be 80 nm, and the NPs had a cubic crystalline structure. PF127-encapsulated Mn
NPs significantly reduce MDA-MB-231 and MCF-7 cell proliferation, while increasing endogenous ROS and lowering mitochondrial matrix protein levels. DAPI, EtBr/AO dual staining, and Annexin-V-FITC-based flow cytometry analysis revealed that PF127-coated Mn
NP-treated breast cancer cells exhibit nuclear damage and apoptotic cell death, resulting in cell cycle arrest in the S phase. Furthermore, PF127-encapsulated Mn
NPs show strong antimicrobial efficacy against various strains. As a result, we can conclude that PF127-coated Mn
NPs may be effective as future anticancer agents and treatment options for breast cancer.
Metal oxide nanoparticles have been found to selectively target the tumor cells while non-toxic to the normal cells. Leukemia is one of the widespread and deadly cancers in adults, as well as the ...most common cancer in children. Recently, the nanoparticles have evolved as a simple, economic, effective, and ecologically sound strategy among the known nanoparticle synthesis techniques. In the present study, the structural, optical, and antibacterial effects of nickel cobalt-codoped Tin oxide nanoparticles (SnNiCoO2 NPs) formulated by the green process and the anticancer potential of SnNiCoO2 NPs in Molt-4 cells have been studied. The cytotoxic potential of the NPs against Molt-4 cells was estimated by MTT assay. The ROS and MMP levels were measured using fluorescent dyes and the changes in morphology and nuclei were noted using AO/EB staining. CAT, SOD, MDA, and GSH), and Proinflammatory Cytokines (TNF-α and IL1β) were also studied. The activity of caspase-3, −9, and −8 levels was examined to analyze the apoptotic mechanism. The XRD patterns of SnNiCoO2 NPs revealed a tetragonal structure. The SnNiCoO2 NPs was revealed a diameter of 126 nm by the DLS study. The morphology and elemental composition were studied using FESEM and EDAX spectra. In the FT-IR study, the O-sn-O stretching band was found to be 615 and 542 cm-1. The antimicrobial potential of the SnNiCoO2 NPs was examined against S. aureus, E. coli, and C. Albicans strains. A tremendous reduction in the viability of MOLT-4 cells at concentration-dependent mode witnessed the cytotoxic potential of the formulated NPs. The augmented ROS accumulation, depletion of MMP status, depleted antioxidants, and increased proinflammatory cytokines (TNF-α and IL1β) were noted on the NPs exposed cells. Furthermore, the increased expressions of caspase-3, −9, and −8 was also noted in the NPs treated MOLT-4 cells. Hence, the outcomes suggest that the formulated SnNiCoO2 NPs had remarkably potent antimicrobial and anticancer properties and could potentially prove beneficial in cancer treatment. Induces mitochondrial oxidative stress with nickel–cobalt-codoped tin oxide nanoparticles from Psidium guajava, which is a potential drug candidate for the antibiotic, antifungal, and anticancer activities of plant-based nanoparticles.
The present study aims to study the cytotoxicity of ZnO-TiO2-Chitosan-Amygdalin nanocomposites (ZnO-TiO2-Chitosan-Amygdalin) on T lymphoblast cancer cells (MOLT-4). In a study, nanocomposites ...containing 2.5 to 15 µg/ml MTT were screened for their anticancer activity. Its anticancer properties were significantly higher than those of other nanocomposites with an IC50 value of 10.34 µg/ml. We studied the mechanism of action for cytotoxic cell death by fluorescence microscopy using Acridine Orange/EtBr (AO/EtBr) and Rhodamine 123 staining procedures. Using DCFH-DA, ZnO-TiO2-Chitosan-Amygdalin nanocomposites were analyzed to determine ROS production. The change in apoptotic protein expression for the 24 h following treatment with MOLT-4 cells for Caspase-3, 8, and 9. Nanocomposites containing ZnO-TiO2-Chitosan-Amygdalin increased the number of early and late apoptotic cells in MOLT-4 cells. ZnO-TiO2-Chitosan-Amygdalin nanocomposites also enhanced mitochondrial apoptosis through Caspase cascade signaling. MOLT-4 cells phosphorylated Caspase cascade in response to ZnO-TiO2-Chitosan-Amygdalin nanocomposites. Compared to the control group, the cancer cells treated with ZnO-TiO2-Chitosan-Amygdalin nanocomposites significantly arrest the proliferation and induces cleavage of pro-apoptotic proteins which leads to apoptotic cell death. Accordingly, ZnO-TiO2-Chitosan-Amygdalin nanocomposites might be effective against T lymphoblast cancer.
Abstract
Background
The association between previous spontaneous abortion and preeclampsia is not yet fully understood. The current study was conducted to assess the association between previous ...spontaneous abortion and preeclampsia among pregnant women in Sudan.
Methods
A case–control study (involving 180 women in each study group) was conducted at Saad Abuelela Hospital, Khartoum, Sudan. The cases were pregnant women with preeclampsia, while the control group included healthy pregnant women. The participants’ sociodemographic, obstetric, and clinical characteristics were assessed via a questionnaire.
Results
There was no significant difference in the age, parity, education level, employment status, blood group, body mass index, and hemoglobin level between the patient and control groups. Forty (22.2%) women with preeclampsia and 68 (37.8%) women in the control group had a history of spontaneous abortion (
p
= 0.001).
Multivariate logistic regression analysis (adjusted) revealed that women with a history of spontaneous abortion had a lower risk of preeclampsia than those without a history of spontaneous abortion adjusted odds ratio (AOR) = 0.44, 95% confidence interval (CI) = 0.26‒0.73. However, women with a history of preeclampsia had a higher risk of recurrence of preeclampsia (AOR = 1.92, 95% CI = 1.11‒3.32).
Conclusion
The present study revealed that previous spontaneous abortion reduced the risk of preeclampsia by 59.0%.
Background: The success of food products is significantly influenced by their development, aimed at achieving a new product characterized by excellent flavor, distinctive color, desirable texture, ...and widespread consumer acceptance. In line with these objectives, the product "al-Khawada" has been innovatively developed. This popular food item is crafted from millet flour and ghee to meet consumer preferences and expectations.Objective: The objective of our study was to enhance al-khawada by refining its flavor, color, and texture, ultimately improving overall liking degree. Acceptance is evaluated through hedonic tests to assess the overall liking and degree of liking for individual sensory attributes. This was achieved by incorporating either olive oil or Nigella sativa into the formulation.Methods: In this study, al-khawada was formulated with the addition of 1% and 2% olive oil, known for its richness in fat and vitamins. This variation is recognized for its ease of digestion. Additionally, black seed was incorporated at 1% and 2%, contributing seeds with high antioxidant content and numerous essential vitamins. The standard al-khawada was utilized as the control group in this comparative analysis.Results: The results of the panalists were analyzed using the SAS program and showed that the addition of olive oil by 1% and 2% in the manufacturing of al-khawada received the satisfaction of the panelists in terms of flavor, color, texture, and overall acceptance while the addition of black seed by 1% and 2% did not have the satisfaction of the panelists in terms of flavor, color, texture, and overall acceptance.Conclusion:The study recommended the need to develop an al-khawada product by adding olive oil at concentration of around 1% and 2% because of its good flavor, distinctive color, desirable texture, and good overall liking. Other studies are needed to increase the ratios of acceptance of al-khawada product to give good flavor, distinctive color, and desired texture.Keywords: Al-khwada, Millet, Ghee, Olive oil, Black cumin seed
Food security in Sudan: the case of Kassala state Samia Satti Osman Mohamed Nour; Eltayeb Mohamedain Abdalla
World journal of science, technology and sustainable development,
11/2021, Volume:
18, Issue:
4
Journal Article
Peer reviewed
PurposeDifferent from the previous studies in the Sudanese literature, this study aims to examine the incidence and of food security, the variation in households' food insecurity between localities ...and the adaptation and survival strategy in Kassala State as a case study of Eastern Sudan.Design/methodology/approachThis paper uses the measurement of Household Food Insecurity Access Scale (HFIAS) and uses new primary data from a Food Security Household Survey in Kassala State (2019) and uses the descriptive analysis to discuss the measurement of HFIAS, the incidence of food security, the variation in households' food insecurity between localities and the adaptation and survival strategy in Kassala State.FindingsThe authors find that the majority of household (77%) are food-insecure of various degrees, with 32.9% being severely food-insecure, while some households are food-secure (23%). The authors find support for their hypothesis that there will be variation in households' food insecurity between localities that most probably relate to variation in the distribution of monthly income between localities. In particular, the authors find that most households in rural areas are severely food-insecure.Originality/valueThis paper provides a significant contribution to the Sudanese and international literature because it discusses the incidence of food insecurity in Sudan. Different from the two other accompanying papers that focused on the determinants of food security in Kassala State using the measurement of HFIAS and determinants of production of food and consumption of food in Kassala State, this paper focuses on the incidence of food security in Kassala State using the measurement of HFIAS.
PurposeThis paper aims to discuss the determinants of food security in Kassala state using the measurement of Household Food Insecurity Access Scale (HFIAS). We use the measurement of HFIAS and use ...new primary data from a food security household survey in Kassala state (2019).Design/methodology/approachThis paper focuses on the determinants of food security in Kassala state using the measurement of Household Food Insecurity Access Scale (HFIAS), using new primary data from a food security household survey in Kassala state (2019) and using the multinomial logistic regression analysis and both ordered logit and ordered probit regression to examine the determinants of food security.FindingsOur results are in support of our hypothesis that the significant determinants of household food insecurity are family-owned production (that negatively affects the probabilities of household being food insecure), household income (that negatively affects HFIAS). We observe that the effects of family-owned production on household food insecurity are particularly significant in the case of mildly and moderately food insecurity. We explain that the other factors that affect the household food insecurity include improvement in the level of agricultural services, marketing, banking services and road characteristics that reduce HFIAS. We find a gender gap related to food security in the sense that male-headed households produce more food compared to female-headed households and also families headed by males are more likely food secure. Therefore, the major policy implication from our results is the importance of increasing households income and enhancing family own production of food to eliminate food insecurity.Originality/valueThis paper provides a significant contribution to the Sudanese and international literature because it discusses the determinants of food security in Kassala state. Different from the two other accompanying papers that focused on the incidence of food security in Kassala state using the measurement of Household Food Insecurity Access Scale (HFIAS) and the determinants of production of food and consumption of food in Kassala state, this paper focuses on the determinants of food security in Kassala state using the measurement of HFIAS and using new primary data from a food security household survey in Kassala state (2019). We fill the gap in the Sudanese literature because we provide a more interesting analysis of the determinants of food security in Kassala state. Our analysis is useful from policy perspective since we provide useful policy recommendations to enhance food security through agricultural development in Kassala state.
