SARS-CoV-2 infected pregnant women are at increased risk of severe COVID-19 than non-pregnant women and have a higher risk of adverse pregnancy outcomes like intrauterine/fetal distress and preterm ...birth. However, little is known about the impact of SARS-CoV-2 infection on maternal and neonatal immunological profiles. In this study, we investigated the inflammatory and humoral responses to SARS-CoV-2 in maternal and cord blood paired samples. Thirty-six pregnant women were recruited at delivery at Hospital Sant Joan de Déu, Barcelona, Spain, between April-August 2020, before having COVID-19 available vaccines. Maternal and pregnancy variables, as well as perinatal outcomes, were recorded in questionnaires. Nasopharyngeal swabs and maternal and cord blood samples were collected for SARS-CoV-2 detection by rRT-PCR and serology, respectively. We measured IgM, IgG and IgA levels to 6 SARS-CoV-2 antigens (spike S, S1, S2, receptor-binding domain RBD, nucleocapsid N full-length and C-terminus), IgG to N from 4 human coronaviruses (OC43, HKU1, 229E and NL63), and the concentrations of 30 cytokines, chemokines and growth factors by Luminex. Mothers were classified as infected or non-infected based on the rRT-PCR and serology results. Sixty-four % of pregnant women were infected with SARS-CoV-2 (positive by rRT-PCR during the third trimester and/or serology just after delivery). None of the newborns tested positive for rRT-PCR. SARS-CoV-2 infected mothers had increased levels of virus-specific antibodies and several cytokines. Those with symptoms had higher cytokine levels. IFN-α was increased in cord blood from infected mothers, and in cord blood of symptomatic mothers, EGF, FGF, IL-17 and IL-15 were increased, whereas RANTES was decreased. Maternal IgG and cytokine levels showed positive correlations with their counterparts in cord blood. rRT-PCR positive mothers showed lower transfer of SARS-CoV-2-specific IgGs, with a stronger effect when infection was closer to delivery. SARS-CoV-2 infected mothers carrying a male fetus had higher antibody levels and higher EGF, IL-15 and IL-7 concentrations. Our results show that SARS-CoV-2 infection during the third trimester of pregnancy induces a robust antibody and cytokine response at delivery and causes a significant reduction of the SARS-CoV-2-specific IgGs transplacental transfer, with a stronger negative effect when the infection is closer to delivery.
The effects of blanching and drying treatments on stability, physical properties, and antioxidant activity of apple pomace polyphenols were evaluated. Blanched and unblanched apples were extracted, ...and the pomace was dried in a cabinet dryer at a speed of 3 m/s at 50 °C, 60 °C, 70 °C, and 80 °C. The color, total phenolics, flavonoids, individual polyphenolic compounds, anthocyanins, and total antioxidant activity were analyzed. The blanching process caused a major retention in color, total polyphenolic content, and total flavonoid content for fresh apple pomace when compared with fresh unblanched pomace. Drying of either fresh blanched or fresh unblanched pomace caused a significant reduction (
P
< 0.05) in total polyphenol and flavonoid content leading to a reduction in the total antioxidant activity. When compared with the unblanched treatment, drying the blanched pomace at 80 °C resulted in a product with significant amounts of total phenolics, flavonoids, and antioxidant activity. The individual phenolic compounds were significantly increased (
P
< 0.05) in blanched pomace that was not dried when compared with unblanched samples. Drying blanched apple pomace did not cause a significant change in the concentration of individual polyphenolic compounds, but drying unblanched apple pomace caused a reduction in the concentrations of epicatechin and caffeic acid, with an important reduction in
p
-coumaric acid at temperatures higher than 60 °C. However, the drying process caused a significant reduction in the antioxidant capacity. Therefore, a combination of blanching and drying processes for apple pomace results in a product that maintains antioxidant capacity.
Background and Aims
Bacterial infections are common and severe in cirrhosis, but their pathogenesis is poorly understood. Dysfunction of liver macrophages may play a role, but information about their ...function in cirrhosis is limited. Our aims were to investigate the specific profile and function of liver macrophages in cirrhosis and their contribution to infections. Macrophages from human cirrhotic livers were characterized phenotypically by transcriptome analysis and flow cytometry; function was assessed in vivo by single photon emission computerized tomography in patients with cirrhosis. Serum levels of specific proteins and expression in peripheral monocytes were determined by ELISA and flow cytometry. In vivo phagocytic activity of liver macrophages was measured by spinning disk intravital microscopy in a mouse model of chronic liver injury.
Approach and Results
Liver macrophages from patients with cirrhosis overexpressed proteins related to immune exhaustion, such as programmed death ligand 1 (PD‐L1), macrophage receptor with collagenous structure (MARCO), and CD163. In vivo phagocytic activity of liver macrophages in patients with cirrhosis was markedly impaired. Monocytes from patients with cirrhosis showed overexpression of PD‐L1 that paralleled disease severity, correlated with its serum levels, and was associated with increased risk of infections. Blockade of PD‐L1 with anti‐PD‐L1 antibody caused a shift in macrophage phenotype toward a less immunosuppressive profile, restored liver macrophage in vivo phagocytic activity, and reduced bacterial dissemination.
Conclusion
Liver cirrhosis is characterized by a remarkable impairment of phagocytic function of macrophages associated with an immunosuppressive transcriptome profile. The programmed cell death receptor 1/PD‐L1 axis plays a major role in the impaired activity of liver macrophages. PD‐L1 blockade reverses the immune suppressive profile and increases antimicrobial activity of liver macrophages in cirrhosis.
En la actualidad existen un sinfín de herramientas que son utilizadas como complemento de la tecnología educativa para asistir y favorecer el aprendizaje. En este artículo se describe el resultado de ...dos estudios exploratorios realizados durante un año escolar 2016/2017 entre estudiantes de la asignatura de matemática de la Escuela Secundaria Federal No. 2 “Julio Zarate” de la ciudad de Xalapa de Enríquez, Veracruz, México. El objetivo de los estudios fue descubrir cuales pares de estudiantes en una actividad colaborativa, obtenían mayores ganancias de aprendizaje haciendo uso de un Sistema Tutor Inteligente. La metodología consistió en trabajar con estudiantes de primero y segundo año de esta secundaria a los cuales se les aplicaron una batería de instrumentos como pre y post test para medir el conocimiento inicial y final, así como el test de colaboración y de estilos de aprendizaje utilizados como instrumentos de recogida de datos. Obteniendo resultados que muestran que los estudiantes con el mismo estilo de aprendizaje que trabajan en pares, consiguen significativamente mayores ganancias de aprendizaje (mayor puntaje en su evaluación del pre al post test) a diferencia de aquellos que trabajan estilos de aprendizaje diferentes. Por lo que se concluye que para formar equipos en parejas para realizar una actividad colaborativa, los estudiantes deben coincidir con el mismo estilo de aprendizaje.
Introduction: The World Health Organization (WHO) has called that climate change is a threat to public health, it is therefore necessary to know the perception of risk to climate change specifically ...on future health professionals. Materials and Methods: Descriptive quantitative research was undertaken with stratified random sampling. Chi2 analysis and logistic regression were performed to identify factors associated with sensitive risk perception of climate change and increased climate change to infectious diseases. Results: By Chi2 was found that about 69% of students perceive climate change as harmful and between 50 and 59% of them perceive that infectious diseases will increase as a result of climate change. By logistic regression statistical significance was observed that students who belong to the sixth semester of study or higher semesters are 60% more likely to recognize that they can get sick from climate change, 63% more likely to perceive climate change as harmful, were associated 2.2 times plus the increase in global temperature to increase in patients with yellow fever and dengue, also have 58% more likely to relate the increase in cases of cholera and malaria, with respect to students belonging to lower semesters. Discussion: Students of different health programs that belong to the sixth semester of study or higher perceived climate change as a threat to public health and associated with increased infectious diseases, thus, can be considered health students as they progress in academic semesters perceive a greater risk of climate change as a threat to public health and an increase in sick for climate-sensitive infectious diseases. Conclusions: It can be concluded that students in health sciences at the University of Magdalena as found in higher semesters acquire a greater perception of risk from the impact of climate change on public health and the increase of infectious diseases.
La práctica psicopedagógica se considera una actuación más para promocionar la transformación escolar. En este estudio de caso cualitativo, realizado en un centro rural agrupado (CRA), se pretende ...conocer las funciones de la psicopedagoga, comprobar su enfoque de intervención y constatar su grado de incidencia en el centro a favor de la inclusión. Los resultados muestran una clara orientación hacia el enfoque clínico debido a las demandas, de parte de la comunidad, al rol de experto que se le asigna y a la visión de déficit respecto a las NEE. No obstante, existen elementos en el centro que favorecen la escuela inclusiva.
Many organisms are capable of growing faster than they do. Restrained growth rate has functionally been explained by negative effects on lifespan of accelerated growth. However, the underlying ...mechanisms remain elusive. Telomere attrition has been proposed as a causal agent and has been mostly studied in endothermic vertebrates. We established that telomeres exist as chromosomal‐ends in a model insect, the field cricket Gryllus campestris, using terminal restriction fragment and Bal 31 methods. Telomeres comprised TTAGGn repeats of 38 kb on average, more than four times longer than the telomeres of human infants. Bal 31 assays confirmed that telomeric repeats were located at the chromosome‐ends. We tested whether rapid growth between day 1, day 65, day 85, and day 125 is achieved at the expense of telomere length by comparing nymphs reared at 23°C with their siblings reared at 28°C, which grew three times faster in the initial 65 days. Surprisingly, neither temperature treatment nor age affected average telomere length. Concomitantly, the broad sense heritability of telomere length was remarkably high at ~100%. Despite high heritability, the evolvability (a mean‐standardized measure of genetic variance) was low relative to that of body mass. We discuss our findings in the context of telomere evolution. Some important features of vertebrate telomere biology are evident in an insect species dating back to the Triassic. The apparent lack of an effect of growth rate on telomere length is puzzling, suggesting strong telomere length maintenance during the growth phase. Whether such maintenance of telomere length is adaptive remains elusive and requires further study investigating the links with fitness in the wild.
Abstract We test the impact of gravitational lensing on the lifetime estimates of seven high-redshift quasars at redshift z ≳ 6. The targeted quasars are identified by their small observed proximity ...zone sizes, which indicate extremely short quasar lifetimes ( t Q ≲ 10 5 yr). However, these estimates of quasar lifetimes rely on the assumption that the observed luminosities of the quasars are intrinsic and not magnified by gravitational lensing, which would bias the lifetime estimates toward younger ages. In order to test the possible effects of gravitational lensing, we obtain high-resolution images of the seven quasars with the Hubble Space Telescope and look for signs of strong lensing. We do not find any evidence of strong lensing, i.e., all quasars are well described by point sources, and no foreground lensing galaxy is detected. We estimate that the strong-lensing probabilities for these quasars are extremely small (∼1.4 × 10 −5 ) and show that weak lensing changes the estimated quasar lifetimes by only ≲0.2 dex. We thus confirm that the short lifetimes of these quasars are intrinsic. The existence of young quasars indicates a high obscured fraction, radiatively inefficient accretion, and/or flickering lightcurves for high-redshift quasars. We further discuss the impact of lensing magnification on measurements of black hole masses and Eddington ratios of quasars.
Currently there are no approved vaccines or specific therapies to prevent or treat Zika virus (ZIKV) infection. We interrogated a library of FDA-approved drugs for their ability to block infection of ...human HuH-7 cells by a newly isolated ZIKV strain (ZIKV MEX_I_7). More than 20 out of 774 tested compounds decreased ZIKV infection in our in vitro screening assay. Selected compounds were further validated for inhibition of ZIKV infection in human cervical, placental, and neural stem cell lines, as well as primary human amnion cells. Established anti-flaviviral drugs (e.g., bortezomib and mycophenolic acid) and others that had no previously known antiviral activity (e.g., daptomycin) were identified as inhibitors of ZIKV infection. Several drugs reduced ZIKV infection across multiple cell types. This study identifies drugs that could be tested in clinical studies of ZIKV infection and provides a resource of small molecules to study ZIKV pathogenesis.
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•774 FDA-approved drugs screened for anti-Zika virus activity in a human hepatoma cell line•Over 20 compounds showed anti-Zika virus activity•Selected compounds validated in human neural stem cells and primary amnion cells
Currently there is no approved therapy to treat Zika virus (ZIKV) infection. Barrows et al. present a screen of FDA-approved drugs for anti-ZIKV activity in a hepatoma cell line. Selected compounds from the more than 20 identified candidates were validated in human neural stem cells and primary amnion cells.
Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and ...bacterial behavior in complex environments. We designed a variety of plasmids, each bearing one of eight unique, constitutively expressed fluorescent protein genes in conjunction with one of four different antibiotic resistance combinations. The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green-yellow, yellow, orange, red, and far-red fluorescent proteins, respectively, were combined with selectable markers conferring tetracycline, gentamicin, kanamycin, and/or chloramphenicol resistance. These constructs were cloned into three different plasmid backbones: a broad host-range plasmid, a Tn
transposon delivery plasmid, and a Tn
transposon delivery plasmid. The utility of the plasmids and transposons was tested in bacteria from the phyla Actinobacteria, Proteobacteria, and Bacteroidetes. We were able to tag representatives from the phylum Proteobacteria at least via our Tn
transposon delivery system. The present study enables labeling bacteria with a set of plasmids available to the community. One potential application of fluorescently-tagged bacterial species is the study of bacteria-bacteria, bacteria-host, and bacteria-environment interactions.
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