Aim : To investigate the acute effects of the ingestion of a fructose-containing beverage combined with fat on postprandial lipoprotein metabolism. Methods : Twelve young healthy Japanese women with ...apolipoprotein E phenotype 3/3 were enrolled in this study. At each of four sessions, the subjects ingested one of four sugar beverages containing fructose and/or glucose (total : 0.5g/kg body weight) combined with OFTT cream (1g/kg, 0.35g/kg as fat) in a randomized crossover design. The four sugar beverages were as follows : 100% (w/w) fructose (F100) , 90% fructose+10% glucose (F90G10) , 55% fructose+45% glucose (F55G45) and 100% glucose (G100) . Venous blood samples were obtained at baseline and 0.5, one, two, four and six hours after ingestion. Results : The serum concentrations of TG in the F100, F90G10 and F55G45 trials were significantly higher than each fasting value at two and four hours, and returned to baseline at six hours, except in the F100 trial. The concentrations at four hours and the incremental areas under the curve for the hepatic triglyceride-rich lipoprotein-triglyceride (VLDL-TGTM) levels in the F100 and F90G10 trials were significantly higher and larger, respectively, than those observed in the G100 trial. Meanwhile, the concentrations of RLP-TG and apolipoprotein B-48 peaked at two hours in the G100 trial, versus four hours in the other trials, and did not return to baseline at six hours, except in the G100 trial. At four hours, the ΔapoB48 tended to be higher in the F100 trial than in the G100 trial. Conclusions : The ingestion of a high-fructose-containing beverage with fat cream delays the clearance of chylomicron and its remnant derived from the intestine and enhances the secretion of triglyceride-rich lipoprotein particles from the liver, thereby inducing postprandial lipidemia, even in young healthy women.
Objectives:
Screening for MYO15A mutations was carried out using a large cohort to clarify the frequency and clinical characteristics of patients with MYO15A (DFNB3) mutations in a hearing loss ...population.
Methods:
Genetic analysis of 63 previously reported deafness genes based on massively parallel DNA sequencing (MPS) in 1120 Japanese hearing loss patients from 53 otorhinolaryngology departments was performed. Detailed clinical features of the patients with MYO15A mutations were then collected and analyzed.
Results:
Eleven patients from 10 families were found to have compound heterozygosity for MYO15A. Audiograms showed profound or high frequency hearing loss, with some patients showing progressive hearing loss. Age at onset was found to vary from 0 to 14 years, which seemed to be associated with the mutation. Four children underwent bilateral cochlear implantation for congenital hearing loss, with all showing good results.
Conclusion:
Mutations in the MYO15A gene are a notable cause of nonsyndromic hearing loss. MPS technology successfully detected mutations in relatively rare deafness genes such as MYO15A.
Aim: To investigate the acute effects of the simultaneous ingestion of fructose and fat on postprandial lipoprotein metabolism in healthy young women. Methods: Nine young healthy Japanese women with ...a normal weight (body mass index: 18.5≤−< 25 kg/m2), a normal ovarian cycle and an apolipoprotein E 3/3 phenotype were enrolled as participants and studied on four occasions. At each session, the subjects ingested one of four beverages containing either glucose or fructose (0.5 g/kg body weight each) with or without OFTT cream (1 g/kg, 0.35 g/ kg as fat) in a randomized crossover design. Blood samples were collected at baseline and at 0.5, 1, 2, 4 and 6 hours after ingestion. Results: The ingestion of fructose combined with fat led to significantly higher rises in the serum triglyceride (TG), remnant-like particle (RLP)-TG, remnant lipoprotein-cholesterol (RemL-C) and apolipoprotein B-48 (apoB48) concentrations with delayed peaks compared with that observed following ingestion of the other three types of beverages. The incremental area under the curve (ΔAUC)-TG and ΔAUC-apoB48 were larger than those observed for the ingestion of fat only. The serum RLP-TG and apoB48 concentrations returned to the fasting levels (0 hours) at the end of the test (6 hours) following the ingestion of fat only; however, these concentrations did not return to the fasting levels following the intake of fructose combined with fat. Conclusions: These findings suggest a delay in the clearance of intestinal TG-rich lipoproteins, namely chylomicron and its remnant, following the ingestion of fructose combined with fat. The simultaneous ingestion of fructose and fat markedly enhances postprandial exogenous lipidemia in young healthy Japanese women.
Direct generation of a benzyl radical by C-H bond activation of toluenes and the addition reaction of the resulting radical to an electron deficient olefin were developed. The reaction of dimethyl ...fumarate with toluene in the presence of Et(3)B as a radical initiator at reflux afforded 2-benzylsuccinic acid dimethyl ester in good yield.
Essential thrombocythemia(ET)is a rare myeloproliferative disorder characterized by thrombocytosis and a risk of thrombotic and hemorrhagic events. ET rarely occurs simultaneously with colorectal ...cancer. Including our case, only 5 cases of c o l orectal cancer with ET have been reported in Japan. Herein, we report a case of colon cancer in an ET patient who underwent laparoscopic right hemicolectomy. Our perioperative management avoided complications such as thrombosis or bleeding. An 81-year-old woman developed bloody stools. She was previously diagnosed with ET 9 years ago. Aspirin, cilostazol, and hydroxyurea(HU)were prescribed. Colonoscopy revealed a tumor at the ascending colon. Histopathological examination showed a well-differentiated tubular adenocarcinoma. Since the patient had anemia, aspirin and cilostazol were discontinued after diagnosis. HU was discontinued from the day before surgery to 2 days after surgery. Enoxaparin was subcutaneously administered for 1 to 3 days after surgery. Aspirin and cilostazol were resumed on the fourth day post-surgery. The patient could be discharged when her condition stabilizes with no thrombosis and bleeding after 8 days.
Aim: To investigate the acute effects of the ingestion of a fructose-containing beverage combinedwith fat on postprandial lipoprotein metabolism. Methods: Twelve young healthy Japanese women with ...apolipoprotein E phenotype 3/3 were enrolled in this study. At each of four sessions, the subjects ingested one of four sugar beverages containing fructose and/or glucose (total: 0.5g/kg body weight) combined with OFTT cream (1g/kg, 0.35g/kg as fat) in a randomized crossover design. The four sugar beverages were as follows: 100% (w/w) fructose (F100), 90% fructose+10% glucose (F90G10), 55% fructose+45% glucose (F55G45) and 100% glucose (G100). Venous blood samples were obtained at baseline and 0.5, one, two, four and six hours after ingestion. Results: The serum concentrations of TG in the F100, F90G10 and F55G45 trials were significantlyhigher than each fasting value at two and four hours, and returned to baseline at six hours, except inthe F100 trial. The concentrations at four hours and the incremental areas under the curve for thehepatic triglyceride-rich lipoprotein-triglyceride (VLDL-TGTM) levels in the F100 and F90G10 trialswere significantly higher and larger, respectively, than those observed in the G100 trial. Meanwhile,the concentrations of RLP-TG and apolipoprotein B-48 peaked at two hours in the G100 trial, versusfour hours in the other trials, and did not return to baseline at six hours, except in the G100 trial.At four hours, the ⊿apoB48 tended to be higher in the F100 trial than in the G100 trial. Conclusions: The ingestion of a high-fructose-containing beverage with fat cream delays the clearance of chylomicron and its remnant derived from the intestine and enhances the secretion of triglyceride-rich lipoprotein particles from the liver, thereby inducing postprandial lipidemia, even in young healthy women.
Objectives:
We sought to elucidate the gene expression profiles of the causative genes as well as the localization of the encoded proteins involved in hereditary hearing loss.
Methods:
Relevant ...articles (as of September 2014) were searched in PubMed databases, and the gene symbols of the genes reported to be associated with deafness were located on the Hereditary Hearing Loss Homepage using localization, expression, and distribution as keywords.
Results:
Our review of the literature allowed us to systematize the gene expression profiles for genetic deafness in the inner ear, clarifying the unique functions and specific expression patterns of these genes in the cochlea and vestibular endorgans.
Conclusions:
The coordinated actions of various encoded molecules are essential for the normal development and maintenance of auditory and vestibular function.
Objectives:
To evaluate the clinical features of Japanese DFNA9 families with mutations of the COCH gene.
Methods:
Mutation screening was performed using targeted next-generation sequencing (NGS) for ...63 previously reported deafness genes. The progression of hearing loss and vestibular dysfunction were evaluated by pure-tone audiometry, caloric testing, cVEMP, and computed dynamic posturography.
Results:
We detected 1 reported mutation of p.G88E and 2 novel mutations of p.I372T and p.C542R. The patients with the novel mutations of p.I372T and p.C542R within the vWFA2 domain showed early onset progressive hearing loss, and the patients with the p.G88E mutation showed late onset hearing loss and acute hearing deterioration over a short period. Vestibular symptoms were reported in the patients with p.G88E and p.C542R. Vestibular testing was performed for the family with the p.G88E mutation. Severe vestibular dysfunction was observed in the proband, and the proband’s son showed unilateral semicircular canal dysfunction with mild hearing loss.
Conclusions:
Targeted exon resequencing of selected genes using NGS successfully identified mutations in the relatively rare deafness gene, COCH, in the Japanese population. The phenotype is compatible with that described in previous reports. Additional supporting evidence concerning progressive hearing loss and deterioration of vestibular function was obtained from our study.
•We analyzed the data management system (DMS) appropriate for international collaboration.•We define the principal requirements for all components of the DMS.•We evaluated application of DMS ...requirements to the JT-60SA experiment.•We evaluated the role network bandwidth and time delay between EU and Japan.
Remote operation and data analysis are becoming key requirements of any fusion devices. In this framework a well-conceived data management system integrated with a suite of analysis and support tools are essential components for an efficient remote exploitation of any fusion device. The following components must be considered: data archiving data model architecture; remote data and computers access; pulse schedule, data analysis software and support tools; remote control room specifications and security issues. The definition of a device-generic data model plays also important role in improving the ability to share solution and reducing learning time. As for the remote control room, the implementation of an Operation Request Gateway has been identified as an answer to security issues meanwhile remotely proving all the required features to effectively operate a device.
Previous requirements have been analyzed for the new JT-60SA tokamak device. Remote exploitation is paramount in the JT-60SA case which is expected to be jointly operated between Japan and Europe. Due to the geographical distance of the two parties an optimal remote operation and remote data-analysis is considered as a key requirement in the development of JT-60SA. It this case the effects of network speed and delay have been also evaluated and tests have confirmed that the performance can vary significantly depending on the technology used.
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