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•First isoconversional-DAEM combined study on pyrolysis kinetics of peel wastes.•Fruit peel wastes showed more complex Ea profiles than agroindustrial byproducts.•All isoconversional ...methods showed a good agreement in the Ea distributions.•Ea profiles from DAEM and isoconversional methods showed the same trends.•Biomass wastes of different characteristics were successfully simulated by DAEM.
In this work, a thermogravimetric analyser was used to assess the pyrolysis kinetics of pineapple, orange and mango peel wastes and agro-industrial by-products, rice husk and pine wood. Five isoconversional methods (KAS, FWO, Starink, Vyazovkin and Friedman) and one model-fitting method (DAEM) accurately fitted the experimental data at three heating rates (5, 10 and 20 °C/min) between 10% and 90% conversion. These methods agree with the trends shown by the activation energy (Ea) distribution calculated, with fluctuations between 150 and 550 kJ/mol. The fluctuations of Ea in the whole range of conversion, in addition to a higher number of relevant reactions obtained by DAEM for fruit peel samples compared to agro-industrial samples, are associated with a higher extractive content in the peels. Kinetic parameters fitted by DAEM were successfully verified at the highest heating rate studied.
Regular mindfulness practice benefits people both mentally and physically, but many populations who could benefit do not practice mindfulness. Virtual Reality (VR) is a new technology that helps ...capture participants' attention and gives users the illusion of "being there" in the 3D computer generated environment, facilitating sense of presence. By limiting distractions from the real world, increasing sense of presence and giving people an interesting place to go to practice mindfulness, Virtual Reality may facilitate mindfulness practice. Traditional Dialectical Behavioral Therapy (DBT®) mindfulness skills training was specifically designed for clinical treatment of people who have trouble focusing attention, however severe patients often show difficulties or lack of motivation to practice mindfulness during the training. The present pilot study explored whether a sample of mindfulness experts would find useful and recommend a new VR Dialectical Behavioral Therapy (DBT®) mindfulness skills training technique and whether they would show any benefit. Forty four participants attending a mindfulness conference put on an Oculus Rift DK2 Virtual Reality helmet and floated down a calm 3D computer generated virtual river while listening to digitized DBT® mindfulness skills training instructions. On subjective questionnaires completed by the participants before and after the VR DBT® mindfulness skills training session, participants reported increases/improvements in state of mindfulness, and reductions in negative emotional states. After VR, participants reported significantly less sadness, anger, and anxiety, and reported being significantly more relaxed. Participants reported a moderate to strong illusion of going inside the 3D computer generated world (i.e., moderate to high "presence" in VR) and showed high acceptance of VR as a technique to practice mindfulness. These results show encouraging preliminary evidence of the feasibility and acceptability of using VR to practice mindfulness based on clinical expert feedback. VR is a technology with potential to increase computerized dissemination of DBT® skills training modules. Future research is warranted.
Radiotherapy-induced gut toxicity is among the most prevalent dose-limiting toxicities following radiotherapy. Prevention of radiation enteropathy requires protection of the small intestine. However, ...despite the prevalence and burden of this pathology, there are currently no effective treatments for radiotherapy-induced gut toxicity, and this pathology remains unclear. The present study aimed to investigate the changes induced in the rat small intestine after external irradiation of the tongue, and to explore the potential radio-protective effects of melatonin gel. Male Wistar rats were subjected to irradiation of their tongues with an X-Ray YXLON Y.Tu 320-D03 irradiator, receiving a dose of 7.5 Gy/day for 5 days. For 21 days post-irradiation, rats were treated with 45 mg/day melatonin gel or vehicle, by local application into their mouths. Our results showed that mitochondrial oxidative stress, bioenergetic impairment, and subsequent NLRP3 inflammasome activation were involved in the development of radiotherapy-induced gut toxicity. Oral treatment with melatonin gel had a protective effect in the small intestine, which was associated with mitochondrial protection and, consequently, with a reduced inflammatory response, blunting the NF-κB/NLRP3 inflammasome signaling activation. Thus, rats treated with melatonin gel showed reduced intestinal apoptosis, relieving mucosal dysfunction and facilitating intestinal mucosa recovery. Our findings suggest that oral treatment with melatonin gel may be a potential preventive therapy for radiotherapy-induced gut toxicity in cancer patients.
In recent decades, many forest die‐off events have been reported in relation to climate‐change‐induced episodes, such as droughts and heat waves. To understand how these extreme climatic events ...induce forest die‐off, it is important to find a tool to standardize the climatic conditions experienced by different populations during a specific climatic event, taking into account the historic climatic conditions of the site where these populations live (bioclimatic niche). In this study, we used estimates of climatic suitability calculated from species distribution models (SDMs) for such purpose. We studied forest die‐off across France during the 2003 heatwave that affected Western Europe, using 2,943 forest inventory plots dominated by 14 single tree species. Die‐off severity was estimated by Normalized Difference Vegetation Index (NDVI) loss using Moderate‐resolution Imaging Spectroradiometer remote sensor imagery. Climatic suitability at the local level during the historical 1979–2002 period (HCS), the episode time (2003; ECS) and suitability deviance during the historical period (HCS‐SD) were calculated for each species by means of boosted regression tree models using the CHELSA climate database and occurrences extracted from European forest inventories. Low HCS‐SD and high mean annual temperature explained the overall regional pattern of vulnerability to die‐off across different monospecific forests. The combination of high historical and low episode climatic suitability also contributed significantly to overall forest die‐off. Furthermore, we observed different species‐specific relationships between die‐off vulnerability and climatic suitability: Sub‐Mediterranean and Mediterranean species tended to be vulnerable in historically more suitable localities (high HCS), whereas Euro‐Siberian species presented greater vulnerability when the hot drought episode was more intense. We demonstrated that at regional scale, past climatic legacy plays an important role in explaining NDVI loss during the episode. Moreover, we demonstrated that SDMs‐derived indexes, such as HCS, ECS and HCS‐SD, could constitute a tool for standardizing the ways that populations and species experience climatic variability across time and space.
Climatic suitability estimated from species distribution models (SDMs) explains monospecific forest die‐off across France during the 2003 extreme hot drought. At this regional scale, past climatic legacy (both historical climatic conditions and historical climatic variability) plays an important role in explaining species die‐off induced by extreme climatic events. Therefore, SDM‐derived indexes constitute a useful tool for standardizing the ways that populations and species experience climatic variability across time and space.
T lymphocyte proliferation and differentiation are controlled by signaling pathways initiated by the T cell antigen receptor. Here we explore how key serine-threonine kinases and their substrates ...mediate T cell signaling and coordinate T cell metabolism to meet the metabolic demands of participating in an immune response.
A plasmonic core–shell gold nanostar/zeolitic‐imidazolate‐framework‐8 (ZIF‐8) nanocomposite was developed for the thermoplasmonic‐driven release of encapsulated active molecules inside living cells. ...The nanocomposites were loaded, as a proof of concept, with bisbenzimide molecules as functional cargo and wrapped with an amphiphilic polymer that prevents ZIF‐8 degradation and bisbenzimide leaking in aqueous media or inside living cells. The demonstrated molecule‐release mechanism relies on the use of near‐IR light coupled to the plasmonic absorption of the core gold nanostars, which creates local temperature gradients and thus, bisbenzimide thermodiffusion. Confocal microscopy and surface‐enhanced Raman spectroscopy (SERS) were used to demonstrate bisbenzimide loading/leaking and near‐IR‐triggered cargo release inside cells, thereby leading to DNA staining.
Triggered release: A thermoresponsive carrier comprising gold nanostars coated with ZIF‐8 stabilized with an amphiphilic polymer was developed for the light‐triggered release of encapsulated cargo inside cells. This nanocomposite, which is stable in aqueous solution (even when stored in cells), combines thermoplasmonic and high drug‐loading capabilities.
Climate change induces changes in plant communities according to species' climatic requirements. These changes can be assessed by community climatic disequilibrium (CD), which corresponds to the ...difference between the climate inferred from the climatic requirements of the species in a community (community‐inferred climate, CIC) and the local observed climate.
We assessed changes in CIC and CD during a long‐term climatic manipulation (warming and drought treatments), embedded within the ongoing trends of climate change, in a Mediterranean shrubland (NE Iberian Peninsula) during 1999–2014. We used plant censuses, species distribution and climate layers since 1979 to create a multivariate environmental space where CIC and CD trends were estimated for 1999–2014.
CD consistently decreased, concomitant with an overall climate change‐derived increase in aridity (higher temperature and lower precipitation). CIC significantly changed during 1999–2014, reflecting the reshuffling of the community composition due to an increase in the abundance of species distributed in warmer, drier and more seasonal localities. Overall, treatments simulating greater climate change did not accelerate the decrease in CD. However, a trend of steeper diminution of CD was observed under warming treatment. In turn, under drought treatment the species less adapted to seasonality and aridity became less abundant. This community tracking of climate did not follow yearly climatic variability; instead, it was detected by the CD trend at the decadal scale.
Synthesis. The procedure developed to measure CD reflects demographic behaviours, thus providing a reliable method to assess the impact of climate change on species and communities. The study demonstrates the current, rapid tracking of Mediterranean woody plant communities to climate change. This tracking results from changes in the abundance of species according to their climatic requirements, inferred from their distribution. However, inertia in demographic processes implies that plant communities do not immediately fit current climate change at local level, as supported by the minor effect of experimentally accentuated climate change.
The procedure developed to measure climatic disequilibrium reflects demographic behaviours, thus providing a reliable method to assess the impact of climate change on species and communities. The study demonstrates the current, rapid tracking of Mediterranean woody plant communities to climate change. This tracking results from changes in the abundance of species according to their climatic requirements, inferred from their distribution, as seen in the trend of the community climatic characterization ((b), (c) panels). However, inertia in demographic processes implies that plant communities do not immediately fit current climate change at local level, as supported by the minor effect of experimentally accentuated climate change.
The current study provides a comprehensive look of the adsorption process of Candida rugosa lipase (CRL) on Ca2Fe2O5 iron oxide nanoparticles (NPs). Protein-support interactions were identified ...across a broad range of pH and ionic strengths (mM) through a response surface methodology, surface charge determination, and spectroscopic and in silico analyses. The maximum quantity of immobilized protein was achieved at an ionic strength of 50 mM and pH 4. However, this condition did not allow for the greatest hydrolytic activity to be obtained. Indeed, it was recorded at acidic pH, but at 150 mM, where evaluation of the recovered activity revealed hyperactivation of the enzyme. These findings were supported by adsorption isotherms performed under different conditions. Based on zeta potential measurements, electrostatic interactions contributed differently to protein-support binding under the conditions tested, showing a strong correlation with experimentally determined immobilization parameters. Raman spectra revealed an increase in hydrophobicity around tryptophan residues, whereas the enzyme immobilization significantly reduced the phenylalanine signal in CRL. This suggests that this residue was involved in the interaction with Ca2Fe2O2 and molecular docking analysis confirmed these findings. Fluorescence spectroscopy showed distinct behaviors in the CRL emission patterns with the addition of Ca2Fe2O5 at pH 4 and 7. The calculated thermodynamic parameters indicated that the contact would be mediated by hydrophobic interactions at both pHs, as well as by ionic ones at pH 4. In this approach, this work adds to our understanding of the design of biocatalysts immobilized in iron oxide NPs.
The study analyzes sensory processing sensitivity and the compassion satisfaction as risk/protective factors against burnout and compassion fatigue, during the first period of the COVID-19 health ...emergency. A sample of 1566 Spanish adult healthcare (n = 694) and education (n = 872) professionals was evaluated. An ad hoc questionnaire for sociodemographic data, and the highly sensitive person scale (HSPS), Maslach burnout inventory (MBI) and professional quality of life scale (ProQOL-vIV) were administered. Burnout and compassion fatigue were observed in the healthcare and education professionals, where personal realization and depersonalization were higher in healthcare and compassion fatigue in education. The protective role of compassion satisfaction was confirmed, as was sensory processing sensitivity as a risk factor, except for its low sensory threshold dimension, which positively influenced personal realization. The findings of this study demonstrate the presence of burnout and compassion fatigue in healthcare and education professionals, displaying compassion fatigue as an emerging psychosocial risk in education, which was made more severe under the conditions of study, which is at the beginning of the COVID-19 pandemic. The importance of incorporating adequate management strategies for high sensitivity, empathy and compassion satisfaction in prevention programs is emphasized.
Generalized Anxiety Disorder (GAD) is a very prevalent disorder in primary care (PC). Most patients with GAD never seek treatment, and those who do seek treatment often drop out before completing ...treatment. Although it is an understudied treatment, Mindfulness-Based Interventions (MBIs) indicate preliminary efficacy for the treatment of GAD symptoms, but many patients with GAD present other associated symptoms (e.g., attention deficits) that complicate the treatment. Virtual Reality DBT
Mindfulness Skills learning has recently been developed to make learning mindfulness easier for patients with emotion dysregulation who have trouble concentrating. Virtual Reality (VR) might serve as a visual guide for practicing mindfulness as it gives patients the illusion of "being there" in the 3D computer generated world. The main goal of this study was to evaluate the effect of two MBIs (a MBI in a group setting alone and the same MBI plus 10 min VR DBT
Mindfulness skills training) to reduce GAD symptoms. A secondary aim was to explore the effect in depression, emotion regulation, mindfulness, and interoceptive awareness. Other exploratory aims regarding the use of VR DBT
Mindfulness skills were also carried out. The sample was composed of 42 patients (roughly half in each group) with GAD attending PC visits. After treatment, both groups of patients showed significant improvements in General Anxiety Disorder measured by the GAD-7 using mixed regression models MBI alone (
= -5.70;
< 0.001;
= -1.36), MBI+VR DBT
Mindfulness skills (
= -4.38;
< 0.001;
= -1.33). Both groups also showed significant improvements in anxiety, depression, difficulties of emotion regulation and several aspects of mindfulness and interoceptive awareness. Patients in the group that received additional 10 min VR DBT Mindfulness Skills training were significantly more adherent to the treatment than those receiving only standard MBI (100% completion rate in MBI + VR vs. 70% completion rate in MBI alone;
= 0.020). Although randomized controlled studies with larger samples are needed, this pilot study shows preliminary effectiveness of MBI to treat GAD, and preliminary evidence that adjunctive VR DBT
Mindfulness Skills may reduce dropouts.
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