In cases where mobility and joint function are impaired after implantation of a THA, weakening of hip movement in both extension/flexion and adduction/abduction may play a role due to shortening of ...the physiological lever arm of the hip muscles. Mechanical factors of influence include the lateral femoral offset, which affects the lever arm, and the antetorsion angle of the hip prosthesis, which affects the anterior femoral offset. This study aimed to investigate the effect of an altered antetorsion angle of the implant on the hip moments and gait patterns of the patient. For this study, 13 patients with a conventional stem on one side and a calcar-guided short stem implanted on the contralateral side were included. To determine the maximum hip moment, tests were performed on a dynamometer in extension/flexion and adduction/abduction in addition to gait analysis. As a control, a comparison was made with data from a reference group of 30 healthy subjects. Both implants showed similar symmetry indices. There was a significant difference between the implants for adduction moments (p < 0.001). The ratios between the directions of moments showed no significant differences. The joint function measured by isokinetic measurements and gait analysis remains comparable to the healthy control group after short stem arthroplasty, but shows slight changes after conventional stem arthroplasty.
Calcium sulfate (CS) can be used as an antibiotically impregnated bone substitute in a variety of clinical constellations. Antibiotically loaded bone substitutes find specific application in ...orthopedic and trauma surgery to prevent or treat bone infections especially in relation to open bone defects. However, its use as a structural bone graft reveals some concerns due to its fast biodegradation. The addition of calcium carbonate and tripalmitin makes CS formulations more resistant to resorption leaving bone time to form during a prolonged degradation process. The aim of the present study was the evaluation of biocompatibility and degradation properties of newly formulated antibiotically impregnated CS preparations. Three different types of CS bone substitute beads were implanted into the tibial metaphysis of rabbits (CS dihydrate with tripalmitin, containing gentamicin (Group A) or vancomycin (Group B); Group C: tobramycin-loaded CS hemihydrate). Examinations were performed by means of x-ray, micro-computed tomography (micro-CT) and histology after 4, 6, 8 and 12 weeks. Regarding biocompatibility of the formulations, no adverse reactions were observed. Histology showed formation of vital bone cells attached directly to the implanted materials, while no cytotoxic effect in the surrounding of the beads was detected. All CS preparations showed osteogenesis associated to implanted material. Osteoblasts attached directly to the implant surface and revealed osteoid production, osteocytes were found in newly mineralized bone. Group C implants (Osteoset
®
) were subject to quick degradation within 4 weeks, after 6–8 weeks there were only minor remnants with little osteogenesis demonstrated by histological investigations. Group A implants (Herafill
®
-G) revealed similar degradation within atleast 12 weeks. In contrast, group B implants (CaSO4-V) were still detectable after 12 weeks with the presence of implant-associated osteogenesis atlatest follow-up. In all of these preparations, giant cells were found during implant degradation on surface and inside of resorption lacunae. None of the analyzed CS preparations triggered contact activation. All implants demonstrated excellent biocompatibility, with implants of Group A and B showing excellent features as osteoconductive and -inductive scaffolds able to improve mechanical stability.
Graphical abstract
High landscape diversity is assumed to increase the number and level of ecosystem services. However, the interactions between ecosystem service provision, disturbance and landscape composition are ...poorly understood. Here we present a novel approach to include uncertainty in the optimization of land allocation for improving the provision of multiple ecosystem services. We refer to the rehabilitation of abandoned agricultural lands in Ecuador including two types of both afforestation and pasture rehabilitation, together with a succession option. Our results show that high compositional landscape diversity supports multiple ecosystem services (multifunction effect). This implicitly provides a buffer against uncertainty. Our work shows that active integration of uncertainty is only important when optimizing single or highly correlated ecosystem services and that the multifunction effect on landscape diversity is stronger than the uncertainty effect. This is an important insight to support a land-use planning based on ecosystem services.
The Transition Radiation Array for Cosmic Energetic Radiation (TRACER) cosmic-ray detector, first flown on long-duration balloon (LDB) in 2003 for observations of the major primary cosmic-ray nuclei ...from oxygen (Z = 8) to iron (Z = 26), has been upgraded to also measure the energies of the lighter nuclei, including the secondary species boron (Z = 5). The instrument was used in another LDB flight in 2006. The properties and performance of the modified detector system are described, and the analysis of the data from the 2006 flight is discussed. The energy spectra of the primary nuclei carbon (Z = 6), oxygen, and iron over the range from 1 GeV amu--1 to 2 TeV amu--1 are reported. The data for oxygen and iron are found to be in good agreement with the results of the previous TRACER flight. The measurement of the energy spectrum of boron also extends into the TeV amu--1 region. The relative abundances of the primary nuclei, such as carbon, oxygen, and iron, above ~10 GeV amu--1 are independent of energy, while the boron abundance, i.e., the B/C abundance ratio, decreases with energy as expected. However, there is an indication that the previously reported E --0.6 dependence of the B/C ratio does not continue to the highest energies.
Substitution of bones is a well-established, necessary procedure to treat bone defects in trauma and orthopedic surgeries. For prevention or treatment of perioperative infection, the implantation of ...resorbable bone substitute materials carrying antibiotics is a necessary treatment. In this study, we investigated the newly formulated calcium-based resorbable bone substitute materials containing either gentamicin (CaSO4-G Herafill-G), vancomycin (CaSO4-V), or tobramycin (Osteoset). We characterized the released antibiotic concentration per unit. Bone substitute materials were implanted in bones of rabbits via a standardized surgical procedure. Clinical parameters and levels of the antibiotic-releasing materials in serum were determined. Local concentrations of antibiotics were measured using antimicrobial tests of bone tissue. Aminoglycoside release kinetics in vitro per square millimeter of bead surface showed the most prolonged release for gentamicin, followed by vancomycin and, with the fastest release, tobramycin. In vivo level in serum detected over 28 days was highest for gentamicin at 0.42 μg/ml, followed by vancomycin at 0.11 μg/ml and tobramycin at 0.04 μg/ml. The clinical parameters indicated high biocompatibility for materials used. None of the rabbits subjected to the procedure showed any adverse reaction. The highest availability of antibiotics at 14.8 μg/g on day 1 in the cortical tibia ex vivo was demonstrated for gentamicin, decreasing within 14 days. In the medulla, vancomycin showed a high level at 444 μg/g on day 1, decreasing continuously over 14 days, whereas gentamicin decreased faster within the initial 3 days. The compared antibiotic formulations varied significantly in release kinetics in serum as well as locally in medulla and cortex.
In the megadiverse tropical mountain forest in the Andes of southern Ecuador, a global biodiversity hotspot, the use of fire to clear land for cattle ranching is leading to the invasion of an ...aggressive weed, the bracken fern, which is threatening diversity and the provisioning of ecosystem services. To find sustainable land use options adapted to the local situation, a profound knowledge of the long-term spatiotemporal patterns of land cover change and its drivers is necessary, but hitherto lacking. The complex topography and the high cloud frequency make the use of remote sensing in this area a challenge. To deal with these conditions, we pursued specific pre-processing steps before classifying five Landsat scenes from 1975 to 2001. Then, we quantified land cover changes and habitat fragmentation, and we investigated landscape changes in relation to key spatial elements (altitude, slope, and distance from roads). Good classification results were obtained with overall accuracies ranging from 94.5% to 98.5% and Kappa statistics between 0.75 and 0.98. Forest was strongly fragmented due to the rapid expansion of the arable frontier and the even more rapid invasion by bracken. Unexpectedly, more bracken-infested areas were converted to pastures than vice versa, a practice that could alleviate pressure on forests if promoted. Road proximity was the most important spatial element determining forest loss, while for bracken the altitudinal range conditioned the degree of invasion in deforested areas. The annual deforestation rate changed notably between periods: ~1.5% from 1975 to 1987, ~0.8% from 1987 to 2000, and finally a very high rate of ~7.5% between 2000 and 2001. We explained these inconstant rates through some specific interrelated local and national political and socioeconomic drivers, namely land use policies, credit and tenure incentives, demography, and in particular, a severe national economic and bank crisis.
Hyperspectral remote sensing is a promising tool for a variety of applications including ecology, geology, analytical chemistry and medical research. This article presents the new hsdar package for R ...statistical software, which performs a variety of analysis steps taken during a typical hyperspectral remote sensing approach. The package introduces a new class for efficiently storing large hyperspectral data sets such as hyperspectral cubes within R. The package includes several important hyperspectral analysis tools such as continuum removal, normalized ratio indices and integrates two widely used radiation transfer models. In addition, the package provides methods to directly use the functionality of the caret package for machine learning tasks. Two case studies demonstrate the package's range of functionality: First, plant leaf chlorophyll content is estimated and second, cancer in the human larynx is detected from hyperspectral data.
In two long-duration balloon flights in 2003 and 2006, the TRACER cosmic-ray detector has measured the energy spectra and the absolute intensities of the cosmic-ray nuclei from boron (Z = 5) to iron ...(Z = 26) up to very high energies. In particular, the second flight has led to results on the energy spectrum of the secondary boron nuclei, and on the boron abundance relative to that of the heavier primary parent nuclei, commonly quantified as the "B/C abundance ratio." The energy dependence of this ratio, now available up to about 2 TeV amu super(-1), provides a measure for the energy dependence of cosmic-ray propagation through the Galaxy, and for the shape of the cosmic-ray source energy spectrum. We use a Leaky-Box approximation of cosmic-ray propagation to obtain constraints on the relevant parameters on the basis of the results of TRACER and of other measurements. This analysis suggests that the source energy spectrum is a relatively soft power law in energy E super(- alpha ), with spectral exponent alpha = 2.37 + or - 0.12, and that the propagation path length Lambda(E) is described by a power law in energy with exponent delta = 0.53 + or - 0.06, but may assume a constant residual value Lambda sub(0) at high energy. The value of Lambda sub(0) is not well constrained but should be less than about 0.8 g cm super(-2). Finally, we compare the data with numerical solutions of a diffusive reacceleration model, which also indicates a soft source spectrum.
Sutures colonized by bacteria represent a challenge in surgery due to their potential to cause surgical site infections. In order to reduce these type of infections antimicrobially coated surgical ...sutures are currently under development. In this study, we investigated the antimicrobial drug octenidine as a coating agent for surgical sutures. To achieve high antimicrobial efficacy and required biocompatibility for medical devices, we focused on optimizing octenidine coatings based on fatty acids. For this purpose, antimicrobial sutures were prepared with either octenidine-laurate or octenidine-palmitate at 11, 22, and 33 μg/cm drug concentration normalized per length of sutures. Octenidine containing sutures were compared to the commercial triclosan-coated suture Vicryl® Plus. The release of octenidine into aqueous solution was analyzed and long-term antimicrobial efficacy was assessed via agar diffusion tests using Staphylococcus aureus. For determining biocompatibility, cytotoxicity assays (WST-1) were performed using L-929 mouse fibroblasts.
In a 7 days elution experiment, octenidine-palmitate coated sutures demonstrated much slower drug release (11 μg/cm: 7%; 22 μg/cm: 5%; 33 μg/cm: 33%) than octenidine-laurate sutures (11 μg/cm: 82%; 22 μg/cm: 88%; 33 μg/cm: 87%). Furthermore sutures at 11 μg/cm drug content were associated with acceptable cytotoxicity according to ISO 10993-5 standard and showed, similar to Vicryl® Plus, relevant efficacy to inhibit surrounding bacterial growth for up to 9 days.
Octenidine coated sutures with a concentration of 11 μg/cm revealed high antimicrobial efficacy and biocompatibility. Due to their delayed release, palmitate carriers should be preferred. Such coatings are candidates for clinical testing in regard to their safety and efficacy.
Quantifying the net carbon flux from land use and land cover changes (fLULCC) is critical for understanding the global carbon cycle and, hence, to support climate change mitigation. However, ...large-scale fLULCC is not directly measurable and has to be inferred from models instead, such as semi-empirical bookkeeping models and process-based dynamic global vegetation models (DGVMs). By definition, fLULCC estimates are not directly comparable between these two different model types. As an important example, DGVM-based fLULCC in the annual global carbon budgets is estimated under transient environmental forcing and includes the so-called loss of additional sink capacity (LASC). The LASC results from the impact of environmental changes on land carbon storage potential of managed land compared to potential vegetation and accumulates over time, which is not captured in bookkeeping models. The fLULCC from transient DGVM simulations, thus, strongly depends on the timing of land use and land cover changes mainly because LASC accumulation is cut off at the end of the simulated period. To estimate the LASC, the fLULCC from pre-industrial DGVM simulations, which is independent of changing environmental conditions, can be used. Additionally, DGVMs using constant present-day environmental forcing enable an approximation of bookkeeping estimates. Here, we analyse these three DGVM-derived fLULCC estimations (under transient, pre-industrial, and present-day forcing) for 12 models within 18 regions and quantify their differences as well as climate- and CO2-induced components and compare them to bookkeeping estimates. Averaged across the models, we find a global fLULCC (under transient conditions) of 2.0±0.6 PgC/yr for 2009–2018, of which ∼40 % are attributable to the LASC (0.8±0.3 PgC/yr). From 1850 onward, the fLULCC accumulated to 189±56 PgC with 40±15 PgC from the LASC. Around 1960, the accumulating nature of the LASC causes global transient fLULCC estimates to exceed estimates under present-day conditions, despite generally increased carbon stocks in the latter. Regional hotspots of high cumulative and annual LASC values are found in the USA, China, Brazil, equatorial Africa, and Southeast Asia, mainly due to deforestation for cropland. Distinct negative LASC estimates in Europe (early reforestation) and from 2000 onward in the Ukraine (recultivation of post-Soviet abandoned agricultural land), indicate that fLULCC estimates in these regions are lower in transient DGVM compared to bookkeeping approaches. Our study unravels the strong dependence of fLULCC estimates on the time a certain land use and land cover change event happened to occur and on the chosen time period for the forcing of environmental conditions in the underlying simulations. We argue for an approach that provides an accounting of the fLULCC that is more robust against these choices, for example by estimating a mean DGVM ensemble fLULCC and LASC for a defined reference period and homogeneous environmental changes (CO2 only).
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