•We proposed the efficacy of DRIAs and dietary supplement on endometriosis.•DRIAs inhibit cell proliferation in human endometriotic stromal cells.•DRIAs reduce inflammatory cytokines and exhibit ...ERβ-mediated activity.•DRIAs reduce the extent of endometriosis-like lesions in a mouse model.•DRIAs might be a potential therapeutic option for management of endometriosis.
Endometriosis is an estrogen-dependent disease, and isoflavones interact with estrogen receptors. The purposes of this study are to investigate the in vitro and in vivo effects of daidzein-rich isoflavone aglycones (DRIAs), dietary supplements, on cellular proliferation in endometriosis. Stromal cells isolated from ovarian endometrioma (OESCs) and normal endometrium (NESCs) were cultured with DRIAs, i.e., each of the DRIA components (daidzein, genistein, or glycitein), or isoflavone glycosides (IG; DRIA precursors). A mouse model of endometriosis was established by transplanting donor-mouse uterine fragments into recipient mice. Our results showed that DRIAs (0.2–20 μM) inhibited the proliferation of OESCs (P < 0.05 for 0.2 μM; P < 0.01 for 2 and 20 μM) but not of NESCs. However, daidzein, genistein, glycitein, and IG did not inhibit their proliferation. DRIA-induced suppression was reversed by inhibition of the estrogen receptor (ER)β by an antagonist, PHTPP, or by ERβ siRNA (P < 0.05), but not by MPP, an ERα antagonist. In OESCs, DRIAs led to reduced expression of IL-6, IL-8, COX-2, and aromatase, as well as reduced aromatase activity, serum glucocorticoid-regulated kinase levels, and PGE2 levels (P < 0.05). Western blot and immunofluorescence assays revealed that DRIAs inhibited TNF-α-induced IκB phosphorylation and p65 uptake into the nuclei of OESCs. In the mouse model, a DRIA-containing feed significantly decreased the number, weight, and Ki-67 proliferative activity of endometriosis-like lesions compared to in mice fed with an IG-containing feed and the control feed (P < 0.01). In conclusion, DRIAs inhibit cellular proliferation in endometriosis, thus representing a potential therapeutic option for the management of endometriosis.
Follipsin, an enzyme that accumulates in the follicular fluid of porcine ovaries during follicular maturation, was purified to apparent homogeneity. The purified enzyme consists of two different ...polypeptide chains having Mr = 45,000 and 32,000 each, associated covalently. The enzyme activity was strongly inhibited by diisopropyl fluorophosphate, benzamidine, leupeptin, and antipain, indicating that follipsin is a serine proteinase. Using synthetic peptide substrates containing 4-methylcoumaryl-7-amide, follipsin was shown to preferentially hydrolyze Arg-X bonds but not Lys-X bonds. The NH2-terminal amino acid sequences of the 45- and 32-kDa polypeptides were highly homologous with those of the heavy and light chains, respectively, of human plasma kallikrein and human factor XIa. Immunological analyses and substrate specificity studies, together with other existing evidence, indicated that follipsin is distinct from kallikrein and factor XIa, thus being a novel type of serine proteinase. Follipsin is immunohistochemically localized in follicular fluid as well as in stroma cells of porcine ovaries. The results strongly suggest that follipsin originates from interstitial cells of the ovarian stroma
InSb thin films with excess antimony were prepared on mica substrates by vacuum evaporation, and their morphology and electrical properties were investigated. Thin films which were coevaporated with ...more than 4% antimony showed p-type conduction. The electrical properties of p-type films were investigated in relation to the amount of excess antimony. As the amount of excess antimony was increased, the impurity concentrations increased and the electron and hole mobilities decreased. From the X-ray diffraction patterns, the films were found to have the InSb-In crystalline structure owing to the segregated indium. A decrease in indium inclusions was observed as the amount of excess antimony was increased.
Necrotizing fasciitis is a potentially fatal clinical disease caused by infection with various bacteria in addition to streptococci, which are common causative agents. We report on a rare case of ...this disease in association with Pasteurella multocida infection. A 58-year-old man had systemic features of shock after a 15-hour history of a painful swelling on the right lower leg. The swelling led to skin blistering and necrosis from which P. multocida was isolated. Those lesions progressed rapidly. The patient also had a history of chronic liver injury as described in previous reports.
Cp*RuCl4 (Cp* = η5-C5Me5) reacts with 2 equiv of dilithium 2,3-naphthalenediamide to afford the dinuclear bridging amido complex (Cp*Ru)2{μ2-(NH)2C10H6} (1b) in moderate yield. Treatment of 1b with ...CO (1 atm) resulted in the incorporation of three molecules of CO into the diruthenium core to give the carbamoyl amido bis(carbonyl) complex Cp*Ru(μ2-CO){μ2-2,3-(CONH)(NH)C10H6}RuCp*(CO) (4).
A 58-year-old Japanese woman who had herpes zoster in association with colitis was successfully treated with intravenously administrated acyclovir. Vesicular lesions with red haloes ranged from the ...left side of her buttock to the left extremity, corresponding to the L4 to S2 dermatomes. Her colitis was considered to have been induced by varicella-zoster virus, based on the facts that the clinical courses were correlated and that the innervation of the affected site of the colon corresponded to an infected dermatome (S2).
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