In Industry 4.0., robots are regarded as one of the key components. In recent years, collaborative robots (cobots) have risen in relevance and have been included in the industry to perform tasks ...alongside humans. Robots have been used in many applications in manufacturing processes; for the scope of this paper, the emphasis on these applications is centered on welding and gluing. These applications need to be performed with specific speed, efficiency, and accuracy to attain optimal welding or bonding to the pieces. An operator cannot maintain such conditions consistently, with minimum variations, for an extended period; hence, robots are a more suitable option to perform those tasks. The robots used for these applications need to be instructed to follow a trajectory to either weld or apply the glue. This path must be programmed on the robot by an operator, and depending on the complexity of the trajectory, it can take up to extended periods of time to set all the required waypoints. There are specialized software environments that contribute to the automation of these tasks; however, the overall cost of the licenses is not affordable if the scale of the project only requires developing and programming trajectories a few times. This paper contains a proposal for an open-source Computer Aided Manufacturing (CAM) software to automatically generate the trajectories needed for the aforementioned welding and gluing applications. The procedure to develop the software starts by selecting the surface that will be welded or to which glue will be applied. The surface determines the model of the trajectory to be followed. Next, a processing system is fed with the individual points that make up the trajectory provided by their selection over the Computer Aided Drawing (CAD) model. This system then creates a program based on URScript® that can be directly uploaded to and executed on the robot. A set of tests is presented to validate the applications and to demonstrate the versatility of the developed trajectory generation system.
We describe and characterize an exceptionally large HIV-1 subtype B transmission cluster occurring in the Comunidad Valenciana (CV, Spain). A total of 1806 HIV-1 protease-reverse transcriptase ...(PR/RT) sequences from different patients were obtained in the CV between 2004 and 2014. After subtyping and generating a phylogenetic tree with additional HIV-1 subtype B sequences, a very large transmission cluster which included almost exclusively sequences from the CV was detected (n = 143 patients). This cluster was then validated and characterized with further maximum-likelihood phylogenetic analyses and Bayesian coalescent reconstructions. With these analyses, the CV cluster was delimited to 113 patients, predominately men who have sex with men (MSM). Although it was significantly located in the city of Valencia (n = 105), phylogenetic analyses suggested this cluster derives from a larger HIV lineage affecting other Spanish localities (n = 194). Coalescent analyses estimated its expansion in Valencia to have started between 1998 and 2004. From 2004 to 2009, members of this cluster represented only 1.46% of the HIV-1 subtype B samples studied in Valencia (n = 5/143), whereas from 2010 onwards its prevalence raised to 12.64% (n = 100/791). In conclusion, we have detected a very large transmission cluster in the CV where it has experienced a very fast growth in the recent years in the city of Valencia, thus contributing significantly to the HIV epidemic in this locality. Its transmission efficiency evidences shortcomings in HIV control measures in Spain and particularly in Valencia.
Staphylococcus epidermidis (SE) is a common cause of bacterial keratitis in certain geographic areas. A high percentage of resistance to methicillin is shown, which gives it cross resistance to ...beta-lactams and sometimes resistance to other antibacterial groups. We analyzed clinical and microbiological variables in patients with infectious keratitis due to SE.
Medical records of 43 patients with suspected infectious keratitis and microbiological confirmation for SE, between October 2017 and October 2020, were retrospectively studied. Clinical characteristics (risk factors, size of lesions, treatment, evolution) and microbiological (susceptibility to antibiotics) were analyzed, and groups of patients with methicillin-resistant (MRSE) and methicillin-susceptible (MSSE) infection were compared.
MRSE was present in 37.2% of infectious keratitis. All isolates were sensitive to vancomycin and linezolid. Rates of resistance to tetracyclines and ciprofloxacin were 50% and 56% in the MRSE group, and 11% and 7% in the MSSE group. The clinical characteristics, including size of lesion, visual axis involvement, inflammation of anterior chamber, presence of risk factors and follow-up time, did not show statistically significant differences between groups.
MRSE is a common cause of infectious keratitis caused by SE and shows a high rate of multidrug resistance. Clinically, it does not differ from MSSE keratitis. Additional work is needed to confirm these findings.
La provincia de Bocas del Toro es recorrida por numerosos ríos, la mayoría tiene sus nacimientos en las estribaciones de la cordillera de Talamanca y cordillera Central, desembocando todo en el mar ...Caribe. Los ríos constituyen un tipo principal de ecosistema acuático que se diferencia por el continuo y rápido flujo de sus aguas; esto crea condiciones especiales para la vida y para la organización de las estructuras y procesos ecológicos básicos 1. El objetivo de esta investigación es evaluar la calidad ecológica y fisicoquímica de cinco afluentes continentales de la Provincia de Bocas del Toro, siendo estas: Río San San, Río Negro, Río Changuinola, Río Oeste y Río Uyama. Se realizó una metodología estándar de muestreo de aguas en el mes de junio 2017, para evaluar aspectos descriptivos del área de estudio, análisis físico (temperatura, turbiedad, color, salinidad, conductividad y TDS) y químicos (pH, amonio, nitrito, nitrato y fosfato). Se concluye que los factores determinantes en la degradación de las propiedades del agua son la utilización de agroquímicos en la agricultura tanto en pequeña escala (finqueros) como a gran escala (industrias), y se indica que a pesar de las alteraciones en algunos parámetros como el amonio, nitrato, conductividad y TDS el estado de conservación de los ríos en Bocas del Toro es relativamente aceptable comparado con otras regiones tropicales.
Recent epidemiological surveillance studies have reported an increase in fungaemia caused by non-Candida albicans species, as well as a decrease in fluconazole susceptibility.
To evaluate changes in ...the epidemiology of fungaemia in Spain comparing data from a new surveillance epidemiological study conducted in 2009 with a previous study carried out from 1997 to 1999 (Pemán J, et al. Eur J Clin Microbiol Infect Dis. 2005).
From January 2009 to February 2010, 44 Spanish hospitals participated in a prospective multicentre fungaemia surveillance study to ascertain whether there have been changes in the epidemiology and fluconazole susceptibility. Susceptibility was determined by the colorimetric method Sensititre Yeast One. Demographic and clinical data and the first isolate of each episode were gathered.
A total of 1,377 isolates from 1,357 fungaemia episodes were collected, 46.7% from patients older than 64years and 8.6% from children less than 1 year old. C. albicans (44.7%), Candida parapsilosis (29.1%), Candida glabrata (11.5%), Candida tropicalis (8.2%), and Candida krusei (1.9%) were the most frequent species isolated. Distribution varied with the geographical area. C. albicans incidence has increased significantly in the last 10years in Cataluña (39.1 vs. 54.7%, P=0.03) and decreased in the Valencian Community (49.1 vs. 34.6%, P=0.002) and Extremadura (58.3 vs. 20%, P=0.01). Susceptibility to fluconazole was similar for all geographical areas, although resistance in C. albicans was ten times greater for patients aged more than 64years. The overall rate of fluconazole resistance (MIC > 32 mg/L) has decreased with respect to that obtained 10years ago (3.7 vs. 2.5%) mainly in C. albicans (3 vs. 1.6%).
In the last ten years, species distribution and fluconazole susceptibility have not significantly changed, although a lower rate of fluconazole resistance has been observed. Species distribution varies with hospital, hospitalization Unit and geographical area.
HIV infections are still a very serious concern for public heath worldwide. We have applied molecular evolution methods to study the HIV-1 epidemics in the Comunidad Valenciana (CV, Spain) from a ...public health surveillance perspective. For this, we analysed 1804 HIV-1 sequences comprising protease and reverse transcriptase (PR/RT) coding regions, sampled between 2004 and 2014. These sequences were subtyped and subjected to phylogenetic analyses in order to detect transmission clusters. In addition, univariate and multinomial comparisons were performed to detect epidemiological differences between HIV-1 subtypes, and risk groups. The HIV epidemic in the CV is dominated by subtype B infections among local men who have sex with men (MSM). 270 transmission clusters were identified (>57% of the dataset), 12 of which included ≥10 patients; 11 of subtype B (9 affecting MSMs) and one (n = 21) of CRF14, affecting predominately intravenous drug users (IDUs). Dated phylogenies revealed these large clusters to have originated from the mid-80s to the early 00 s. Subtype B is more likely to form transmission clusters than non-B variants and MSMs to cluster than other risk groups. Multinomial analyses revealed an association between non-B variants, which are not established in the local population yet, and different foreign groups.
Expansion of the CRF19_cpx Variant in Spain Patiño Galindo, Juan Angel; Torres-Puente, Manoli; Gimeno, Concepción ...
Journal of clinical virology,
08/2015, Volume:
69
Journal Article
Peer reviewed
Highlights • HIV-1CRF19_cpx is a more pathogenic HIV-1 variant mainly found in Cuba. • We checked for the presence of HIV-1CRF19_cpx in The Valencian Region (Spain). • We report for the first time ...HIV-1CRF19_cpx transmission groups outside Cuba. • Resistance mutations were present in most of the sequences from transmission groups. • CRF19_cpx is an emerging HIV variant in Spain, affecting native MSM.
The increase in sexually transmitted infections (STI) caused by Neisseria gonorrhoeae (NG) worldwide, together with the decrease in antibiotic susceptibility, forced us to understand the epidemiology ...of gonococcal infection.
The GONOvig project analyzed, comparatively following CLSI and EUCAST criteria, the antibiotic susceptibility of 227 NG strains collected in thirteen representative hospitals of the Valencia Community (CV) between 2013 and 2018. Additionally, molecular typing of 175 strains using the NG multi-antigen sequence typing technique (NG-MAST) was performed.
High rates of resistance to tetracycline (38.2% by CLSI and 50.9% by EUCAST) and ciprofloxacin (49.1% CLSI and 54% EUCAST), and low percentages of resistance to spectinomycin (0%), cefixime (0.5% CLSI but 5.9% EUCAST), and ceftriaxone (1.5% CLSI and 2.4% EUCAST) were detected. Azithromycin resistance was 6% (both CLSI and EUCAST). Molecular analysis revealed the presence of 86 different sequence types (ST), highlighting ST2992 (7.4%), ST3378 (6.9%), ST2400 (4.6%) and ST13288 (6.9%), which was associated with resistance to cefixime (P=.031). The main genogroups (G) were G1407 (13.1%), G2992 (10.3%), G2400 (6.3%) and G387 (3.4%). G1407 and G2400 were associated with resistance to ciprofloxacin (P<.03).
Low resistance to ceftriaxone, a worrying resistance to azithromycin and a wide variety of circulating sequence types have been detected, some of which show correlation with certain resistance profiles.
El aumento de las infecciones de transmisión sexual producidas por Neisseria gonorrhoeae (NG) a nivel mundial, junto con la disminución de la susceptibilidad antibiótica, obliga a profundizar en la epidemiologia de la infección gonocócica.
El proyecto GONOvig analizó, comparativamente siguiendo criterios CLSI y EUCAST, la sensibilidad antibiótica de 227 cepas de NG recogidas en trece hospitales representativos de la Comunidad Valencia (CV) entre los años 2013 y 2018. Adicionalmente, se pudo realizar la tipificación molecular de 175 cepas mediante la técnica NG multi-antigen sequence typing (NG-MAST).
Se detectaron elevadas tasas de resistencia a tetraciclina (38,2% por CLSI y 50,9% por EUCAST) y ciprofloxacino (49,1% CLSI y 54% EUCAST), y bajos porcentajes de resistencia a espectinomicina (0%), cefixima (0,5% CLSI pero 5,9% EUCAST) y ceftriaxona (1,5% CLSI y 2,4% EUCAST). La resistencia a azitromicina fue del 6% (tanto CLSI como EUCAST). El análisis molecular reveló la presencia de 86 secuenciotipos (ST) distintos, destacando el ST2992 (7,4%), ST3378 (6,9%), ST2400 (4,6%) y ST13288 (6,9%) el cual presentaba asociación con resistencia a cefixima (P=,031). Los genogrupos (G) mayoritarios fueron el G1407 (13,1%), G2992 (10,3%), G2400 (6,3%) y G387 (3,4%); G1407 y G2400 mostraron asociación con resistencia a ciprofloxacino (P<,03).
Se ha detectado una baja resistencia a ceftriaxona, una preocupante resistencia a azitromicina y una gran variedad de secuenciotipos circulantes, algunos de los cuales presentan correlación con determinados perfiles de resistencia.
The objectives of this work were to know the prevalence of methicillin-resistant S. aureus (MRSA) infections in the paediatric population of our health department, to describe the risk factors for ...infection by MRSA compared to those produced by methicillin-susceptible S. aureus (MSSA) and to know the antibiotic sensitivity profile of MRSA and MSSA isolates.
A retrospective, descriptive and analytical study of infections produced by MRSA versus those produced by MSSA was carried out during the years 2014 to 2018. Risk factors for MRSA infection were studied using a binary logistic regression model.
162 patients with S. aureus infections were identified. Of these, 25 (15.4%) were MRSA. The highest percentages of MRSA infection occurred among children who required hospital admission (23.4%). In the univariate analysis the need of hospital admission, antibiotic treatment in the last 3 months, the kind of infection and past MRSA infection or colonisation reached statistical significance. However, only the need of hospital admission and antibiotic treatment in the last 3 months maintained statistical significance in the binary logistic regression model. Correct antibiotic treatment was only prescribed in 26.7% of the MRSA infection cases admitted to the hospital.
Our results suggest the need to review empirical local treatment regimen using drugs active against MRSA in infections of probable staphylococcal origin admitted to the hospital, especially if they have received antibiotic treatment in the last 3 months.
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