Inverted papilloma is an epithelial neoplasm of the lateral nasal wall and adjacent sinuses characterized by a marked propensity for recurrence and a significant association with carcinoma. In this ...retrospective study we present 31 cases treated by our departments between 1982 and 1999. The aim was to compare our results to those of other authors especially regarding surgical management. The male to female ratio of these patients was 2:1 and most patients were in the 6th and 7th decades of life. Conservative surgery was used in most cases as the initial treatment. The overall recurrence rate was low and there were 3 cases associated with carcinoma. We conclude that the results of conservative surgery in selected cases are comparable to those using radical methods. A review of the literature is presented and particular attention is dedicated to the literature concerning analysis of p53 expression, HPV and Epstein-Barr infection and apoptosis in inverted papilloma.
To isolate and genotype Borrelia burgdorferi genospecies in serum samples of Croatian patients with erythema migrans.
DNA isolates from sera of patients with erythema migrans were analyzed by nested ...polymerase chain reaction (PCR), amplifying a segment of flagellin gene with primers encompassing the conserved region of the gene. To screen PCR products for heterogeneity, we performed single-stranded conformation polymorphism (SSCP) analysis. The samples showing differences in SSCP patterns were sequenced, and the sequence compared in the GeneBank for sequence homology with known Borrelia burgdorferi genospecies. We also constructed phylogenetic tree of all known borrelial sequences.
The nested PCR method using specially designed flagellin gene primers, achieved the sensitivity of 10 genome copies (0.01 pg of purified Borrelia burgdorferi DNA from culture) by dilution analysis. The assay specificity was confirmed by amplification of a part of the flagellin gene from different bacterial species. The primer pairs successfully amplified only Borrelia burgdorferi flagellin gene. The genome of Borrelia burgdorferi sensu lato was detected in the sera of all 10 tested patients with erythema migrans. Sequence data and phylogenetic analysis confirmed that all amplified samples belonged to Borrelia afzelii genospecies.
Phylogenetic tree analysis placed the borrelial isolates together with Borrelia afzelii sequences into a single group. This finding was additionally supported by sequence homology analysis, which produced a homology score of 99%. In patients with erythema migrans who come from the northwest Croatia, an endemic area for Lyme borreliosis, Borrelia afzelii was the cause of skin manifestations of Lyme borreliosis.
Inverted papilloma is an epithelial neoplasm of the lateral nasal wall and adjacent
sinuses characterized by a marked propensity for recurrence and a significant association
with carcinoma In this ...retrospective study we present 31 cases treated by our departments
between 1982 and 1999. The aim was to compare our results to those of other
authors especially regarding surgical management. The male to female ratio of these
patients was 2:1 and most patients were in the 6th and 7th decades of life. Conservative
surgery was used in most cases as the initial treatment. The overall recurrence rate was
low and there were 3 cases associated with carcinoma. We conclude that the results of
conservative surgery in selected cases are comparable to those using radical methods. A
review of the literature is presented and particular attention is dedicated to the literature
concerning analysis of p53 expression, HPV and Epstein-Barr infection and apoptosis
in inverted papilloma.
