A highly hydrophobic protein with six transmembrane structure that is coded by the candidate tumor suppressor gene 101F6 located in the human chromosome 3p.21.3 and a possible member of the ...cytochrome b 561 protein family was expressed, purified, and characterized in its functional form for the first time. The protein was heterologously expressed in methylotrophic yeast Pichia pastoris as a fusion protein containing a C-terminal thrombin-specific sequence and an 8-His residue tag. Purification was achieved by ion exchange chromatography on DEAE-Sepharose and affinity chromatography on Ni-NTA-Sepharose. SDS-PAGE analysis revealed a single protein band with an estimated molecular weight of 26 kDa, while Western blot and MALDI-TOF-MS analysis confirmed the presence of the cytochrome b561 specific sequence in the protein. The 101F6 protein was found to be reducible by ascorbate efficiently and to have two midpoint potentials at +89.5 and +13.1 mV, slightly lower than the corresponding values of +155 and +62 mV, respectively, of bovine adrenal cytochrome b 561, despite a lower conservation of the putative ascorbate binding site sequence in the 101F6 protein. The "modified motif 1" sequence unique in 101F6 protein may be responsible for other molecular functions, such as protein-protein interactions, in the endoplasmic membranes.
Reservation of adequate food is a major concern for many developing countries worldwide. Governments of those countries also want to meet the demand of food in the long term, especially during the ...period of natural calamity. For low lying region such as Bangladesh, predicting the supply of food is critical. Besides, rice productivity of Bangladesh has also changed due to varying climatic over the last couple of decades. In this paper, a comprehensive analysis has been made to forecast the rice yield of Bangladesh.
A complete model is presented in this article for design optimization of hydraulic fracturing to enhance reservoir production. The model integrates reservoir properties, various design constraints to ...avoid induced formation damage, a hydraulically driven fracture geometry model, a hydrocarbon production model, and an economic model in deciding on the optimum values of various treatment parameters. The design constraints are intended to ensure that an optimum design obtained from the model can be executed in the field using the specified surface equipment and without causing uncontrolled fracture growth and/or initiation of multiple secondary fractures. A pseudo-3D fracture model is improved to establish better compatibility relationships between reservoir properties, treatment parameters, and fracture growth. Features of Genetic Algorithm and Evolutionary Operation are combined to develop a robust optimization algorithm that executes complex interactions between the above-mentioned modules to achieve an optimum design. The capability and robustness of the proposed model is demonstrated by applications to a tight-gas reservoir. By trade-off analysis between production/NPV and treatment cost, it is shown that only a 13% sacrifice of the maximum possible production/net present value (NPV) over ten years can save up to 52% of the fracturing treatment cost. Various other design issues are also investigated by sensitivity analyses.
ZigBee is a protocol for high-level communication under IEEE 802.15.4 standard. ZigBee network specification creates a personal area network for small, less-power consuming radio communication. The ...vast use in device to device communication of ZigBee turns its possibility in the use of the Internet of Things. A large amount of sensor nodes is interconnected through the Coordinator in ZigBee network. The end nodes send a huge amount of sensing data to the Coordinator via intermediary nodes like routers. These huge data creates overhead application traffic load in routers and Coordinator. A load balancing mechanism is necessary for a ZigBee network to contain multiple Personal Area Networks (PAN) and switch the threshold PAN loads with simplified network routing. This paper represents a novel ZigBee network model based on Stochastic and Widest Path process for proper traffic orientation, queuing and analyze load minimization issues. The two statistical models are put through in the ZigBee network using Riverbed to reduce and balance the overhead load. The result indicates that applying Stochastic process minimize the Medium Access Control (MAC) layer load as well as the overall ZigBee network load. This research will be helpful in large-scale integration of sensor nodes in a balanced ZigBee network for data load.
Clustered and smarter web mining using semantic web Galib, Syed Md; Rahman, Md Motiur; Shah, Ajay ...
2015 18th International Conference on Computer and Information Technology (ICCIT),
12/2015
Conference Proceeding
Semantic Web which is an extension to the current web 2.0 presents information more magnificently for humans and automated tools as well. It offers an intelligent web service that synchronizes and ...arranges précised data over web in a well-organized modus. The exactitude of fetching relevant data as per the user demand has been a real challenge for traditional web over years in the field of data mining. In this paper, we propose an approach for mapping data over web 3.0 through web ontology and retrieve the précised information through an intelligent agent. The agent provides all the searched data related to the user query from where desired information is found. If the user does not have enough search parameter, mining can be carried out by the information appended by the agent. Our proposed model shortlists the results fetched by traditional search engines such as Google which are semantically related to users' search parameter. A case study of Institutions of Barisal Division is considered to examine the helpfulness of the model.
ZigBee is a wireless standard based on IEEE 802.15.4 specification for a set of communication protocols. It is intended for remote control and sensor applications. ZigBee is designed to use low power ...digital radio signals for personal area networks which require secure networking. The use of the ZigBee protocol accommodates and facilitates for carrying out secure communications, ciphering frames and controlling devices, protecting establishment and transport of cryptographic keys. This paper presents a security enhancement technique for ZigBee by modifying the MAC layer. This work also serves enhanced security processing in Network and Application layer of ZigBee protocol. The improved security mechanism of Network and Application layer has been developed using Radio Frequency Identification (RFID) and proxy firewall techniques respectively. Riverbed v17.5 is used to simulate and scrutinize different ZigBee layers to enrich security. The result of this study provides reliable and stable ZigBee protocol scheme to end users. Furthermore, the paper shows security adoption in MAC, Network, and Application layers of ineligible nodes, packets, and data blocks. The benefits of the work include end-to-end security with confidentiality, data packet integrity, message and device authentication.
