Glyphosate is a herbicide widely used to kill weeds both in agricultural and non-agricultural landscapes. Its reproductive toxicity is related to the inhibition of a StAR protein and an aromatase ...enzyme, which causes an in vitro reduction in testosterone and estradiol synthesis. Studies in vivo about this herbicide effects in prepubertal Wistar rats reproductive development were not performed at this moment. Evaluations included the progression of puberty, body development, the hormonal production of testosterone, estradiol and corticosterone, and the morphology of the testis. Results showed that the herbicide (1) significantly changed the progression of puberty in a dose-dependent manner; (2) reduced the testosterone production, in semineferous tubules’ morphology, decreased significantly the epithelium height (
P
< 0.001; control = 85.8 ± 2.8 μm; 5 mg/kg = 71.9 ± 5.3 μm; 50 mg/kg = 69.1 ± 1.7 μm; 250 mg/kg = 65.2 ± 1.3 μm) and increased the luminal diameter (
P
< 0.01; control = 94.0 ± 5.7 μm; 5 mg/kg = 116.6 ± 6.6 μm; 50 mg/kg = 114.3 ± 3.1 μm; 250 mg/kg = 130.3 ± 4.8 μm); (4) no difference in tubular diameter was observed; and (5) relative to the controls, no differences in serum corticosterone or estradiol levels were detected, but the concentrations of testosterone serum were lower in all treated groups (
P
< 0.001; control = 154.5 ± 12.9 ng/dL; 5 mg/kg = 108.6 ± 19.6 ng/dL; 50 mg/dL = 84.5 ± 12.2 ng/dL; 250 mg/kg = 76.9 ± 14.2 ng/dL). These results suggest that commercial formulation of glyphosate is a potent endocrine disruptor in vivo, causing disturbances in the reproductive development of rats when the exposure was performed during the puberty period.
To understand the complex relationship governing transcript abundance and the level of the encoded protein, we integrate genome-wide experimental data of ribosomal density on mRNAs with a novel ...stochastic model describing ribosome traffic dynamics during translation elongation. This analysis reveals that codon arrangement, rather than simply codon bias, has a key role in determining translational efficiency. It also reveals that translation output is governed both by initiation efficiency and elongation dynamics. By integrating genome-wide experimental data sets with simulation of ribosome traffic on all Saccharomyces cerevisiae ORFs, mRNA-specific translation initiation rates are for the first time estimated across the entire transcriptome. Our analysis identifies different classes of mRNAs characterised by their initiation rates, their ribosome traffic dynamics, and by their response to ribosome availability. Strikingly, this classification based on translational dynamics maps onto key gene ontological classifications, revealing evolutionary optimisation of translation responses to be strongly influenced by gene function.
Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the ...footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform acquisition and control software, our miniscope is a versatile addition to the expanding tool chest of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.
ABSTRACT
While the kinematics of galaxies up to z ∼ 3 have been characterized in detail, only a handful of galaxies at high redshift (z > 4) have been examined in such a way. The Atacama Large ...Millimeter/submillimeter Array (ALMA) Large Program to INvestigate C ii at Early times (ALPINE) survey observed a statistically significant sample of 118 star-forming main-sequence galaxies at z = 4.4–5.9 in C ii158 $\mu$m emission, increasing the number of such observations by nearly 10×. A preliminary qualitative classification of these sources revealed a diversity of kinematic types (i.e. rotators, mergers, and dispersion-dominated systems). In this work, we supplement the initial classification by applying quantitative analyses to the ALPINE data: a tilted ring model (TRM) fitting code (3Dbarolo), a morphological classification (Gini-M20), and a set of disc identification criteria. Of the 75 C ii-detected ALPINE galaxies, 29 are detected at sufficient significance and spatial resolution to allow for TRM fitting and the derivation of morphological and kinematic parameters. These 29 sources constitute a high-mass subset of the ALPINE sample ($M_*\gt 10^{9.5}\, \mathrm{M}_{\odot }$). We robustly classify 14 of these sources (six rotators, five mergers, and three dispersion-dominated systems); the remaining sources showing complex behaviour. By exploring the G-M20 of z > 4 rest-frame far-infrared and C ii data for the first time, we find that our 1 arcsec ∼ 6 kpc resolution data alone are insufficient to separate galaxy types. We compare the rotation curves and dynamical mass profiles of the six ALPINE rotators to the two previously detected z ∼ 4–6 unlensed main-sequence rotators, finding high rotational velocities (∼50–250 km s−1) and a diversity of rotation curve shapes.
We present a novel robotic grasp controller that allows a sensorized parallel jaw gripper to gently pick up and set down unknown objects once a grasp location has been selected. Our approach is ...inspired by the control scheme that humans employ for such actions, which is known to centrally depend on tactile sensation rather than vision or proprioception. Our controller processes measurements from the gripper's fingertip pressure arrays and hand-mounted accelerometer in real time to generate robotic tactile signals that are designed to mimic human SA-I, FA-I, and FA-II channels. These signals are combined into tactile event cues that drive the transitions between six discrete states in the grasp controller: Close, Load, Lift and Hold, Replace, Unload, and Open. The controller selects an appropriate initial grasping force, detects when an object is slipping from the grasp, increases the grasp force as needed, and judges when to release an object to set it down. We demonstrate the promise of our approach through implementation on the PR2 robotic platform, including grasp testing on a large number of real-world objects.
This paper presents a new class of thin, dexterous continuum robots, which we call active cannulas due to their potential medical applications. An active cannula is composed of telescoping, ...concentric, precurved superelastic tubes that can be axially translated and rotated at the base relative to one another. Active cannulas derive bending not from tendon wires or other external mechanisms but from elastic tube interaction in the backbone itself, permitting high dexterity and small size, and dexterity improves with miniaturization. They are designed to traverse narrow and winding environments without relying on ldquoguidingrdquo environmental reaction forces. These features seem ideal for a variety of applications where a very thin robot with tentacle-like dexterity is needed. In this paper, we apply beam mechanics to obtain a kinematic model of active cannula shape and describe design tools that result from the modeling process. After deriving general equations, we apply them to a simple three-link active cannula. Experimental results illustrate the importance of including torsional effects and the ability of our model to predict energy bifurcation and active cannula shape.
Key points
Purkinje cells in the cerebellum integrate input from sensory organs with that from premotor centres.
Purkinje cells use a variety of sensory inputs relaying information from the ...environment to modify motor control.
Here we investigated to what extent the climbing fibre inputs to Purkinje cells signal mono‐ or multi‐sensory information, and to what extent this signalling is subject to recent history of activity.
We show that individual climbing fibres convey multiple types of sensory information, together providing a rich mosaic projection pattern of sensory signals across the cerebellar cortex.
Moreover, firing probability of climbing fibres following sensory stimulation depends strongly on the recent history of activity, showing a tendency to homeostatic dampening.
Cerebellar Purkinje cells integrate sensory information with motor efference copies to adapt movements to behavioural and environmental requirements. They produce complex spikes that are triggered by the activity of climbing fibres originating in neurons of the inferior olive. These complex spikes can shape the onset, amplitude and direction of movements and the adaptation of such movements to sensory feedback. Clusters of nearby inferior olive neurons project to parasagittally aligned stripes of Purkinje cells, referred to as ‘microzones’. It is currently unclear to what extent individual Purkinje cells within a single microzone integrate climbing fibre inputs from multiple sources of different sensory origins, and to what extent sensory‐evoked climbing fibre responses depend on the strength and recent history of activation. Here we imaged complex spike responses in cerebellar lobule crus 1 to various types of sensory stimulation in awake mice. We find that different sensory modalities and receptive fields have a mild, but consistent, tendency to converge on individual Purkinje cells, with climbing fibres showing some degree of input‐specificity. Purkinje cells encoding the same stimulus show increased events with coherent complex spike firing and tend to lie close together. Moreover, whereas complex spike firing is only mildly affected by variations in stimulus strength, it depends strongly on the recent history of climbing fibre activity. Our data point towards a mechanism in the olivo‐cerebellar system that regulates complex spike firing during mono‐ or multi‐sensory stimulation around a relatively low set‐point, highlighting an integrative coding scheme of complex spike firing under homeostatic control.
Key points
Purkinje cells in the cerebellum integrate input from sensory organs with that from premotor centres.
Purkinje cells use a variety of sensory inputs relaying information from the environment to modify motor control.
Here we investigated to what extent the climbing fibre inputs to Purkinje cells signal mono‐ or multi‐sensory information, and to what extent this signalling is subject to recent history of activity.
We show that individual climbing fibres convey multiple types of sensory information, together providing a rich mosaic projection pattern of sensory signals across the cerebellar cortex.
Moreover, firing probability of climbing fibres following sensory stimulation depends strongly on the recent history of activity, showing a tendency to homeostatic dampening.
A detailed and comprehensive simulation model of a H2 production plant based on the Sorption Enhanced Reforming (SER) process of natural gas has been developed in this work. Besides thermodynamic ...advantages related to the shift of reforming equilibrium, SER technology features an intrinsic CO2 capture that can be of interest in environmentally constrained economies. The model comprises natural gas treatment, H2 and CO2 compression, as well as H2 purification with an adsorption unit that has been integrated within the SER process by using the off-gas for sorbent regeneration. A complete thermal integration has been also performed between the available hot gas streams in the plant, so that high pressure steam is generated and used to generate power in a steam cycle.
A comprehensive comparison with conventional H2 production technologies based on fired tubular reforming (FTR) has been made by defining a proper set of performance indexes. The investigation allowed to set the optimal design parameters and operating conditions of the SER plant and conclude that a better hydrogen production efficiency can be attained when reactors are designed for atmospheric pressure operations rather than pressurized (3 bar) conditions. The SER plant with optimized design parameters (reformer operating temperature at 923 K and a steam-to-carbon ratio in the initial charge around 2) shows considerable improvements on the carbon capture ratio (around 99% for the SER case vs. 85% for the FTR case) and on the natural gas to hydrogen conversion efficiency (by around 15 percentage points) in comparison to the FTR based process featuring CO2 capture. On the whole, a higher natural gas to hydrogen conversion efficiency by 9–15 percentage points, or by 3–6 percentage points when considering credits for steam and electricity import/export, have resulted for the SER plant operating at atmospheric pressure in comparison to the conventional hydrogen production process.
•Detailed process simulation of an SER-based plant for H2 production is presented.•The effect of the SER operating conditions on plant performance indexes is analysed.•Almost 100% of carbon capture ratio is obtained in every case analysed.•9–15 % points of difference in the H2 efficiency compared to conventional reforming.•3–6 % points of equivalent H2 efficiency improvement on conventional reforming.
ABSTRACT
In this work, we analyse the connection between gas availability and the position of a region with respect to the spatially resolved main-sequence (MS) relation. Following the procedure ...presented in Enia et al. (2020), for a sample of five face-on, grand design spiral galaxies located on the MS we obtain estimates of stellar mass and star formation rate surface densities (Σ⋆ and ΣSFR) within cells of 500 pc size. Thanks to H i 21cm and 12CO(2–1) maps of comparable resolution, within the same cells we estimate the surface densities of the atomic (ΣH i) and molecular ($\Sigma _{\rm {H_2}}$) gas and explore the correlations among all these quantities. Σ⋆, ΣSFR, and $\Sigma _{\rm {H_2}}$ define a 3D relation whose projections are the spatially resolved MS, the Kennicutt–Schmidt law and the molecular gas MS. We find that $\Sigma _{\rm {H_2}}$ steadily increases along the MS relation and is almost constant perpendicular to it. ΣH i is nearly constant along the MS and increases in its upper envelope. As a result, ΣSFR can be expressed as a function of Σ⋆ and ΣH i, following the relation log ΣSFR = 0.97log Σ⋆ + 1.99log ΣH i − 11.11. We show that the total gas fraction significantly increases towards the starburst regions, accompanied by a weak increase in star formation efficiency. Finally, we find that H2/H i varies strongly with the distance from the MS, dropping dramatically in regions of intense star formation, where the UV radiation from newly formed stars dissociates the H2 molecule, illustrating the self-regulating nature of the star formation process.
PDF
![The ALPINE-ALMA [C ii] Surv...](http://api.summon.serialssolutions.com/2.0.0/image/issn/XR4PS5YY4K/0035-8711_1365-8711_1365-2966/small "The ALPINE-ALMA [C ii] Survey: kinematic diversity and rotation in massive star-forming galaxies at z ~ 4.4–5.9")
