Introduction
Vocational rehabilitation (VR) emphasizes a need for medical support, rehabilitation and biopsychosocial approach to enable individuals to successfully participate in the workforce. ...Optimal rehabilitation management relies on an in-depth knowledge of the typical spectrum of problems encountered of patients in VR. The International Classification of Functioning, Disability and Health (ICF) is based on a universal conceptual model and provides a holistic view of functioning of the lived experience of people such as those undergoing VR. The objectives of this study are to describe the functioning and health of persons undergoing VR and to identify the most common problems around work and in VR using the ICF as the reference framework.
Methods
An empirical cross-sectional multicenter study was conducted using convenience sampling from March 2009 to March 2010. Data were collected using a Case Record Form rated by health professionals which was based on an extended version of the ICF Checklist containing 292 ICF categories and sociodemographic information.
Results
152 patients with various health conditions participated. We identified categories from all four ICF components: 24 for
body functions
, six for
body structures
, 45 for
activities and participation,
and 25 for
environmental factors
.
Conclusions
Our study identified a multitude of ICF categories that describe functioning domains and which represent the complexity of VR. Such a comprehensive approach in assessing patients in VR may help to understand and customize the process of VR in the clinical setting and to enhance multidisciplinary communication.
Objective
About 3% of newborns show malformations, with about 20% of the affected having genetic causes. Clarification of genetic diseases in postnatal diagnostics was significantly improved with ...high‐throughput sequencing, in particular through whole exome sequencing covering all protein‐coding regions. Here, we aim to extend the use of this technology to prenatal diagnostics.
Method
Between 07/2018 and 10/2020, 500 pregnancies with fetal ultrasound abnormalities were analyzed after genetic counseling as part of prenatal diagnostics using WES of the fetus and parents.
Results
Molecular genetic findings could explain ultrasound abnormalities in 38% of affected fetuses. In 47% of these, disease‐causing de novo variants were found. Pathogenic variants in genes with autosomal recessive or X‐linked inheritance were detected in more than one‐third (70/189 = 37%). The latter are associated with increased probability of recurrence, making their detection important for further pregnancies. Average time from sample receipt to report was 12 days in the recent cases.
Conclusion
Trio exome sequencing is a useful addition to prenatal diagnostics due to its high diagnostic yield and short processing time (comparable to chromosome analysis). It covers a wide spectrum of genetic changes. Comprehensive interdisciplinary counseling before and after diagnostics is indispensable.
Key points
What's already known about this topic?
It is known that about 20% of malformations in newborns can be associated with genetic causes.
Whole‐exome sequencing, and especially trio exome sequencing, is an established and successful method in postnatal genetic diagnostics. Diagnostic yield for trio exome sequencing is around 37%.
What does this study add?
We show that trio exome sequencing is a fast and comprehensive method in prenatal diagnostics with diagnostic yield similar to that of postnatal trio exome sequencing.
We provide case solution rates for different phenotypic observations from 19% for abnormalities of internal organs up to 52% for skeletal malformations.
Complex coacervates are liquid-liquid phase separated systems, typically containing oppositely charged polyelectrolytes. They are widely studied for their functional properties as well as their ...potential involvement in cellular compartmentalization as biomolecular condensates. Diffusion and partitioning of solutes into a coacervate phase are important to address because their highly dynamic nature is one of their most important functional characteristics in real-world systems, but are difficult to study experimentally or even theoretically without an explicit representation of every molecule in the system. Here, we present an explicit-solvent, molecular dynamics coarse-grain model of complex coacervates, based on the Martini 3.0 force field. We demonstrate the accuracy of the model by reproducing the salt dependent coacervation of poly-lysine and poly-glutamate systems, and show the potential of the model by simulating the partitioning of ions and small nucleotides between the condensate and surrounding solvent phase. Our model paves the way for simulating coacervates and biomolecular condensates in a wide range of conditions, with near-atomic resolution.
Martini 3 force field can capture the experimental trends of complex coacervates and can be extended to gain physical insight on the mechanisms that drive the formation of LLPS.
Due to their short wavelength, X-rays can in principle be focused down to a few nanometres and below. At the same time, it is this short wavelength that puts stringent requirements on X-ray optics ...and their metrology. Both are limited by today's technology. In this work, we present accurate at wavelength measurements of residual aberrations of a refractive X-ray lens using ptychography to manufacture a corrective phase plate. Together with the fitted phase plate the optics shows diffraction-limited performance, generating a nearly Gaussian beam profile with a Strehl ratio above 0.8. This scheme can be applied to any other focusing optics, thus solving the X-ray optical problem at synchrotron radiation sources and X-ray free-electron lasers.
Background
Controlled donation after circulatory determination of death (cDCD) seems an effective way to mitigate the critical shortage of available organs for transplant worldwide. As a recently ...developed procedure for organ retrieval, some questions remain unsolved such as the uncertainty regarding the effect of functional warm ischemia time (FWIT) on organs´ viability.
Methods
We developed a multicenter prospective cohort study collecting all data from evaluated organs during cDCD from 2017 to 2020. All the procedures related to cDCD were performed with normothermic regional perfusion. The analysis included organ retrieval as endpoint and FWIT as exposure of interest. The effect of FWIT on the likelihood for organ retrieval was evaluated with Relative distribution analysis.
Results
A total amount of 507 organs´ related information was analyzed from 95 organ donors. Median donor age was 62 years, and 63% of donors were male. Stroke was the most common diagnosis before withdrawal of life‐sustaining therapy (61%), followed by anoxic encephalopathy (21%). This analysis showed that length of FWIT was inversely associated with organ retrieval rates for liver, kidneys, and pancreas. No statistically significant association was found for lungs.
Conclusions
Results showed an inverse association between functional warm ischemia time (FWIT) and retrieval rate. We also have postulated optimal FWIT's thresholds for organ retrieval. FWIT for liver retrieval remained between 6 and less than 11 min and in case of kidneys and pancreas, the optimal FWIT for retrieval was 6 to 12 min. These results could be valuable to improve organ utilization and for future analysis.
Multicenter prospective cohort study collecting all data from evaluated organs during controlled Donor Cardiac Death.
A total amount of 507 organs´ related information was analyzed from 95 organ donors.
Optimal Functional Warm Ischemia Time's (FWIT) thresholds for organ retrieval remained between 6 and less than 11 min for liver and in case of kidneys and pancreas, the optimal FWIT for retrieval was 6 to 12 min.
The human cytomegalovirus UL99-coded pp28 is a myristoylated phosphoprotein located in the virion tegument domain, which resides between the capsid and envelope. A previous study has demonstrated ...that BADsubUL99, a pp28-deficient mutant virus, fails to assemble enveloped virus particles. Capsids, coated with tegument proteins, accumulate in the cytoplasm of mutant virus-infected cells. This phenotype indicates that pp28 is required for the acquisition of an envelope; it presumably acts by directing tegument-associated capsids to bud through an intracellular membrane derived from the cell's secretory apparatus that has been modified to contain viral transmembrane glycoproteins. Here we demonstrate that BADsubUL99 can spread from cell to cell, even though highly sensitive assays fail to detect infectious virus progeny in cultures of infected fibroblasts. We propose that, in the absence of pp28, tegument-coated capsids might nevertheless bud through cellular membranes, including the plasma membrane. If this suggestion is correct, the enveloped particle could potentially infect an adjacent cell to mediate the cell-to-cell spread that is observed. This mode of spread might also occur after infection with wild-type virus, and it could facilitate immune evasion, assuming that the resulting particles do not have a normal complement of virus-coded envelope glycoproteins.
During clathrin-mediated endocytosis, a complex and dynamic network of protein-membrane interactions cooperate to achieve membrane invagination. Throughout this process in yeast, endocytic coat ...adaptors, Sla2 and Ent1, must remain attached to the plasma membrane to transmit force from the actin cytoskeleton required for successful membrane invagination. Here, we present a cryo-EM structure of a 16-mer complex of the ANTH and ENTH membrane-binding domains from Sla2 and Ent1 bound to PIP
that constitutes the anchor to the plasma membrane. Detailed in vitro and in vivo mutagenesis of the complex interfaces delineate the key interactions for complex formation and deficient cell growth phenotypes demonstrate its biological relevance. A hetero-tetrameric unit binds PIP
molecules at the ANTH-ENTH interfaces and can form larger assemblies to contribute to membrane remodeling. Finally, a time-resolved small-angle X-ray scattering study of the interaction of these adaptor domains in vitro suggests that ANTH and ENTH domains have evolved to achieve a fast subsecond timescale assembly in the presence of PIP
and do not require further proteins to form a stable complex. Together, these findings provide a molecular understanding of an essential piece in the molecular puzzle of clathrin-coated endocytic sites.
Ptychographic X‐ray imaging at the highest spatial resolution requires an optimal experimental environment, providing a high coherent flux, excellent mechanical stability and a low background in the ...measured data. This requires, for example, a stable performance of all optical components along the entire beam path, high temperature stability, a robust sample and optics tracking system, and a scatter‐free environment. This contribution summarizes the efforts along these lines to transform the nanoprobe station on beamline P06 (PETRA III) into the ptychographic nano‐analytical microscope (PtyNAMi).
The ptychographic nano‐analytical microscope (PtyNAMi) is designed for in situ/operando high‐resolution imaging in two and three dimensions with high sensitivity and structural, elemental, chemical and electronic contrast.
Inhaltsverzeichnis
Kalina Kupczyńska/ Jadwiga Kita-Huber
Vorwort
Paul Michael Lützeler
Erlebte transnationale Germanistik.
Erfahrungen und Möglichkeiten einer kosmopolitischen
Literaturwissenschaft
...I. Positionen zur Autobiografieforschung
Christian Moser
Körpergraphien.
Automedialität zwischen Schrift, Bild und Spur
Małgorzata Sugiera
(Auto)Biografien der (nicht-)menschlichen Anderen
Robert Walter-Jochum
Nach der Authentizität.
Affekte in autobiografischen Texten und ihre Nutzung
zur Authentizitätssimulation am Beispiel von Ruth Klüger
und Wolfgang Herrndorf
Mateusz Chaberski
Towards Sym(bio)poiesis.
Human-and-Nonhuman Life-Making Practices
in Contemporary Performing Arts
Gudrun Heidemann
Auf den Spuren der Vorfahren oder Nachkommen.
Autogeografik in Katja Petrowskajas Vielleicht Esther,
Tomasz Różyckis Dwanaście stacji (Zwölf Stationen)
und Sabrina Janeschs Katzenberge
Christoph Schmitt-Maaß
Chronik der Verwahrlosung.
Eine andere Geschichte der Moderne im Medium
der Auto-Ethnographie – von Hubert Fichte zu Clemens Meyer
Autobiografische Selbstinszenierungen in literaturgeschichtlicher
Perspektive
Joachim Jacob
Schrift und Bild in der pietistischen Autobiographie
Hermann Korte
Autobiographien um 1800.
Theaterleidenschaft bei Karl Philipp Moritz
und August Wilhelm Iffland
Urszula Bonter
Autobiographische Strategien im pornographischen Roman
des 18. Jahrhunderts
Jan Röhnert /Nils Reichert
„In Gedanken in die Regionen jenseits des Grabes versezt“.
Christian von Massenbachs Memoiren zur Geschichte
des preußischen Staates – ein vergessenes autobiographisches
Großprojekt der Goethezeit und Möglichkeiten seiner Erschließung
Wolfgang Hackl
Mein Leben.
Adalbert Stifters autobiografischer Text als poetischer Schreibakt
Lorella Bosco
Subjektkonstitution und theatralische Sendung.
Autobiographie und Bildungsroman
in Emmy Hennings’ Blume und Flamme und Das flüchtige Spiel
Maria Kłańska
Die „Erinnerungspassagen“ von Ilma Rakusa Mehr Meer (2009).
Ein Beispiel für eine mitteleuropäische Mehrfachidentität?
Karolina Matuszewska
„Ich kann nicht lustiger sein – als ich bin!“
Autobiographische Elemente im Werk Paul Scheerbarts
Mediale Sonderformen I: (Verschiftlichte) Selbstporträts
Monika Schmitz-Emans
Lexika und Wörterbücher als Selbstporträts
Oliver Ruf
Visual Storytelling und Digital Design.
Autobiographie-Theorie und medienästhetische Praxis
von Web-Comics
Katarzyna Norkowska
Selbstinszenierung an den Rändern des Buches?
Zum paratextuellen Aufbau ostdeutscher Autobiographik nach 1989
Monika Szczepaniak
Haas spricht.
Wolf Haas’ automediale Spiele
Piotr Bukowski
„It ain‘t me…“
Bob Dylans autobiographisches Maskenspiel
Carola Hilmes
Ich, Selbst, Jetzt.
Modi autobiographischen Erzählens
Jadwiga Kita-Huber
„Ich als Text“.
Autobiografische Verfahren in den Frankfurter Poetikvorlesungen
2005-2015
Mediale Sonderformen II: Comic-Autobiografie
Ole Frahm
Parodien des Selbst.
Zur Zäsur der Comicgeschichte 1972
Kalina Kupczyńska
Autobiografie, confessional writing und Graphic Memoir
Bernd Dolle-Weinkauff
Autofiktion als Geschichtsschreibung?
Historisches und autobiografisches Erzählen im Comic
Nina Heindl
„Jimmy Corrigan has my grandfather’s hair, Charlie Brown’s eyes
and my self-doubt“.
Autobiografische und autofiktionale Beziehungen
in Chris Wares Comics
Marie Schröer
Arty/fiziell authentisch?!
Autobiografische Performance im Comic
Véronique Sina
Constructing the Gendered Jewish Self.
Geschlecht und Identität in den autobiografischen Comics
von Aline Kominsky Crumb
Autobiografie als existenzieller Text
Katarzyna Jaśtal
Wolfgang Herrndorfs Arbeit und Struktur.
Autobiografisches Schreiben am Rande des Lebens
Joanna Jabłkowska
„Dir bleibt aber nur die Liebe. Die zu dir selbst.“
Autobiographie und Ironie in Martin Walsers Werk
Magdalena Sitarz /Andrzej Pawelec
Dos lid fun oysgehargetn yidishn folk von Yitskhok Katsenelson
als Egodokument
Joanna Drynda
Das autobiographische Ich in Silvia Bovenschens Notizen Älter werden
Dorota Sośnicka
Provokative Selbstinszenierung: Autobiographisches
im Werk Hermann Burgers
Martin A. Hainz
„Wär nicht das Auge sonnenhaft...“
Philologie bzw. Literary Criticism als Autobiographie,
u.a. anhand von Johann W. Goethe und Ralph W. Emerson
Zu den Beiträger_innen
Medien spielen im Kontext der Autobiografie eine tragende Rolle. Zum einen begleiten sie menschliche Biografien, entscheiden über die Intensität der Erinnerung und formen ihre Speicherung. Zum anderen prägen sie die Praxis des autobiografischen Schreibens, indem sie die Kulturtechniken des Erinnerns mitbestimmen. Besonders aufschlussreich sind hierbei intermediale Formen der Autobiografie, etwa Comics oder virtuelle Autobiografien, welche die literarische Produktion nachhaltig verändern. Ist die wachsende Verbreitung der intermedialen Autobiografie ein Symptom für die Veränderung des kulturellen Gedächtnisses überhaupt?
Der Band versammelt diverse Sichtweisen auf die autobiografische Praxis und versteht sich als Versuch, Autobiografie- und Comicforschung einander anzunähern. Das Spektrum reicht von einem Aufriss aktueller Positionen zur Autobiografieforschung über die Darstellung unterschiedlicher Aspekte des Phänomens (experimentelle Tendenzen der historischen Autobiografie sowie neuere mediale Formen der Autobiografie) bis hin zur Frage der existentiellen Dimension autobiografischer Erzählung.
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