Aim: To report the clinical merits and usefulness of minilaparotomy hysterectomy in comparison to the traditional abdominal approach.
Material and Methods: A comparative study to review ...perioperative and postoperative outcomes and complications was conducted in 98 patients undergoing abdominal hysterectomy through Pfannenstiel incision for benign gynecological disease or pre‐invasive pathology. Fifty of these patients (Group I) underwent hysterectomy by minilaparotomy approach (≤5 cm incision) and 48 patients (Group II) had standard/conventional methodology (>6 cm incision).
Results: Though there were no statistically significant differences between the patients' profile as well as the size and weight of the removed uterus in the two groups, the mean operating time was 41.3 min in Group I and 77.5 min in Group II (P < 0.01) and the mean postoperative hospital stay of 3.1 days in Group I was significantly lower than in Group II patients (5.4 days), P < 0.01. There was no major complication or mortality in either of the groups and the composite morbidity encountered was 4% versus 33.33% in Groups I and II, respectively. Two of the patients in Group II required blood transfusion whereas none of the patients in Group I had estimated blood loss over 500 mL.
Conclusion: Minilaparotomy hysterectomy through ≤5 cm Pfannenstiel incision provides an appealing, effective, expeditious, minimal access and less invasive cost‐effective option/alternative to the traditional abdominal approach obviating the need for any additional expensive equipment and, above all, improves upon the perioperative outcome, notwithstanding, whatsoever, on the quality of surgery.
Sequence analysis of Saccharomyces cerevisiae chromosome IX identified a 946 amino acid open reading frame (YIL002C), designated here as INP51 , that has carboxyl- and amino-terminal regions similar ...to mammalian inositol polyphosphate 5-phosphatases and to yeast SAC1 . This two-domain primary structure resembles the mammalian 5-phosphatase, synaptojanin. We report that Inp51p is associated
with a particulate fraction and that recombinant Inp51p exhibits intrinsic phosphatidylinositol 4,5-bisphosphate 5-phosphatase
activity. Deletion of INP51 ( inp51 ) results in a âcold-tolerantâ phenotype, enabling significantly faster growth at temperatures below 15â°C as compared with
a parental strain. Complementation analysis of an inp51 mutant strain demonstrates that the cold tolerance is strictly due to loss of 5-phosphatase catalytic activity. Furthermore,
deletion of PLC1 in an inp51 mutant does not abrogate cold tolerance, indicating that Plc1p-mediated production of soluble inositol phosphates is not
required. Cells lacking INP51 have a 2â4-fold increase in levels of phosphatidylinositol 4,5-bisphosphate and inositol 1,4,5-trisphosphate, whereas cells
overexpressing Inp51p exhibit a 35% decrease in levels of phosphatidylinositol 4,5-bisphosphate. We conclude that INP51 function is critical for proper phosphatidylinositol 4,5-bisphosphate homeostasis. In addition, we define a novel role for
a 5-phosphatase loss of function mutant that improves the growth of cells at colder temperatures without alteration of growth
at normal temperatures, which may have useful commercial applications.
This paper presents a research study examining the acculturation and identity of second-generation Indian Punjabis. It focuses on how they balance their lives between two cultures and reflects on the ...nature of second-generation integration in light of cultural context, situational factors, ethnicity and consumption, presenting the conflicts and tensions experienced by participants. The paper reviews existing propositions with regard to acculturation, ethnicity and consumption and presents an initial conceptualization of the many factors influencing the second generation's consumption process. This conceptualization is refined in light of two key outcomes which emerged from the empirical research. First, we recognize that participants' identities are in constant negotiation. This negotiation is dealt with by harnessing economic, social and cultural capital. This capital is self acquired as well as passed on by the first generation. Second, we suggest the strength of family ties and the strong Punjabi influence in the socialization of these participants causes tensions, leading them to span two, sometimes contradictory, cultures on a daily basis. In particular, we highlight the influence of izatt (family honor) on Punjabi decision making. A revised conceptualization aims to capture the complexities of the acculturative process for the participants. The paper aims to contribute to the current discussion regarding the complexities of acculturation in the twenty-first century.
Manganese (Mn) deficiency has become a serious nutritional problem for wheat grown in alkaline coarse textured soil. The study aimed to investigate post-anthesis Mn partitioning in different wheat ...species. Cultivars of bread wheat (‘PBW509’, ‘DBW17’, ‘PBW550’ and ‘PBW636’); durum wheat (‘PDW291’) and triticale (‘TL2908’) were grown in 6.5 L pots with two treatments of Mn (0 and 50 mg Mn kg ⁻¹ soil) in screen house and harvested at anthesis, 18- days post-anthesis, and maturity to record Mn uptake. Durum cv. ‘PDW291’ retained highest proportion of Mn in its vegetative parts under Mn deficiency resulting into lowest partitioning to the grain and had the lowest grain yield. All bread wheat cv. facilitated superior Mn partitioning to the grain, lesser retention in vegetative organs and higher Mn utilization efficiency, than triticale and durum wheat species. Cultivars producing higher yield on Mn deficit soils are viable alternative to foliar application of Mn.
Renal ischemia is of great clinical interest because of its role in renal failure and renal graft rejection. The purpose of this study was to investigate the therapeutic effects of a combination ...therapy of: n-acetyl cysteine (NAC), a potent antioxidant, sodium nitroprusside (SNP), a nitric oxide donor and phosphormidon (P), an endothelin-1 converting enzyme inhibitor, on tissue protection against renal ischemia/reperfusion injury in the canine model.
In this study, 15-20 kg male dogs were subjected to 90 minutes of warm unilateral renal ischemia after removal of one kidney and then divided into control, ischemia alone and treatment groups. Blood samples were collected from these dogs for measurement of kidney function tests and the kidneys were harvested at different time intervals for morphological evaluation, immunostaining and Tunnel Assay.
Kidney function tests (serum creatinine and blood urea nitrogen BUN) showed a significant difference between untreated and treated groups. ** P <0.01, * P< 0.0001 for treated versus untreated. The protective effect of the combination therapy is also supported by light microscopic studies, immunostaining of renal tissue sections for pro-inflammatory cytokines (TNF-alpha and IFN-gamma), iNOS, and apoptosis by TUNEL assay.
Our results suggest that pre-administration of a combination of NAC, SNP, and P attenuates renal ischemia/reperfusion injury. This has potential application in preservation of donor kidney for transplantation by protecting cells against free radical damage.
Wheat genotypes display differential tolerance to manganese (Mn) deficiency. Growing Mn-efficient cultivars in Mn deficient soil could be effective in improving yields. A pot experiment was conducted ...with eight genotypes grown in Mn deficient soil treated with 0 (no Mn fertilizer) and 50 ppm (50 mg Mn kg
−1
soil applied as mangansese sulfate monohydrate (MnSO
4
.H
2
O) Mn. The genotypes were classified on the basis of grain yield and grain physiological efficiency as efficient and responsive (SAMNYT 410, GLUPRO 200, PBW 621, and BW 9178), efficient and nonresponsive, inefficient and responsive (HD 2967), and inefficient and nonresponsive (PDW 314, PDW 291, and PBW 636). The genotypes in different groups differed in morphophysiological characteristics; efficient and responsive genotypes recorded more leaf area, higher SPAD index, higher Fv/Fm ratio, and longer roots than inefficient and nonresponsive. Efficient and responsive genotypes are desirable by farmers, whereas inefficient and responsive genotypes in a breeding program for their Mn-responsive characteristics.
Consumer research was conducted to evaluate the acceptability of choice and select steaks from the
Longissimus lumborum that were cooked to varying degrees of doneness using demographic information, ...cluster analysis and descriptive analysis. On average, using data from approximately 155 panelists, no differences (
P
>
0.05) existed in consumer acceptability among select and choice steaks, and all treatment means ranged between like slightly and like moderately (6–7) on the hedonic scale. Individual consumers were highly variable in their perception of acceptability and consumers were grouped into clusters (eight for select and seven for choice) based on their preference and liking of steaks. The largest consumer groups liked steaks from all treatments, but other groups preferred (
P
<
0.05) steaks that were cooked to various end-point temperatures. Results revealed that consumers could be grouped together according to preference, liking and descriptive sensory attributes, (juiciness, tenderness, bloody, metallic, and roasted) to further understand consumer perception of steaks that were cooked to different end-point temperatures.
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