We analyzed the research data on antitumor effects of a wide range of ferrocene compounds and discussed possible mechanisms of their bioactivities. Current trends in the study of anticancer effects ...of ferrocene derivatives were considered. Promising ways in the design of low-toxicity anticancer ferrocene-based drugs of new generation were outlined.
—
Interferon-regulated factors play a central role in the activation of the innate immune response. The interferon-regulatory factor 7 (IRF7) is one of the factors that are quickly activated and are ...involved in a cellular response to a viral infection. In this work, monoclonal lines, based on HEK293FT cells defective in the
IRF7
gene, were obtained using a CRISPR-Cas9 genome-editing method. These lines differed in the viability, proliferation rate, and susceptibility to infection with influenza A virus. Transcriptomic analysis of the most susceptible cell clone revealed differential expression of IRF7 factor as well as the other interferon-regulated genes.
The results of studies in which the relationship of the self-organization, physicochemical properties, and bioeffects in dilute aqueous solutions of various biologically active substances sustained ...under natural and reduced levels of external physical fields (geomagnetic and low-frequency electromagnetic), as well as under exposure to ionizing radiation solutions studied by the complex of physicochemical methods, are considered. The established patterns can be used to create a scientific base related to the clarification of the mechanisms of the negative influence of hypomagnetic conditions on the functioning of living organisms necessary to ensure the normal operation of the ecosystem of spacecraft during flights.
The aim
of the study
is
to determine antioxidant, anti-inflammatory and hypolipidemic activities of the extracts from
Cosmos bipinnatus Cav.
three varieties,
obtained by the extraction with 70% ethyl ...alcohol.
Materials and methods.
The antioxidant effect was studied
in vitro
using a model of iron-induced lipid peroxidation in the lecithin liposome system. The study of the anti-inflammatory activity was carried out on 30 male rats of the “Wistar” line. Diclofenac was used as a reference drug (p. o., the dose of 13 mg/kg in terms of the weight of an adult). The substances were administered to animals in the form of an aqueous suspension stabilized with Tween-80. To simulate the inflammatory process, a model of “cotton granuloma” was created. A hypolipidemic activity of the extracts was studied on 36 white male rats of the “Wistar” line. To study the hypolipidemic activity of the studied extracts, a tween model was used to create a hyperlipidemic state in rats, the concentrations of total cholesterol and triglycerides was determined in the serum of the experimental animals.
Results.
The conducted model experiments made it possible to conclude that the alcohol extracts obtained from the dried inflorescences of the
“Dazzler”, “Rosea”
and “
Purity”
varieties
of
Cosmos bipinnatus Cav.
have antioxidant, anti-inflammatory and hypolipidemic kinds of activities. It has been established that the extracts from
the
“Dazzler”
and
“Rosea”
varieties (
Cosmos bipinnatus Cav.)
contribute to a better reduction in the accumulation of peroxide compounds, compared to the extract obtained from the
“Purity”
variety. The data analysis on the anti-inflammatory activity shows that all the studied objects significantly (p = 0.05) reduce the stage of exudation compared with the control group animals by 50% (the «
Purity»
variety), by 52% (the «
Rosea»
variety) and by 40% (the
“Dazzler
” variety).
An experiment on the study of a hypolipidemic activity in the control group of the animals revealed a significant, in relation to the values of the intact group, increase in the cholesterol level of the blood serum by 78%, and in the level of triglycerides (TGCs) – by 64%.
The administration of the extracts obtained from
Cosmos bipinnatus Cav.
“Purity”
,
“Rosea”
,
“Dazzler”
varieties to the animals, led to a decrease in cholesterol in blood serum by 44%, 47%, 50%, and triglycerides by 52%, 52% and 57%, respectively. Both indicators reached the normal level and did not differ significantly from the values in healthy (intact) animals.
Conclusion.
According to the conducted studies, it can be concluded that alcohol extracts obtained from
Cоsmos bipinnatus Cav
., have pronounced antioxidant, anti-inflammatory and hypolipidemic kinds of effect.
It is found that temperature in the range of 25–60 °C affects the size and ζ-potential of dispersed phase domains, as well as specific conductance (χ), pH, UV absorption spectra and fluorescence of ...the succinic acid (SA)—water system with an SA concentration of 1 · 10
−5
mol L
−1
. It was demonstrated that there is an interrelation between non-monotonic temperature dependences of the size and ζ-potential of domains, optical density (
A
260
), fluorescence intensity (λ
ex
= 260 nm, λ
em
= 425 nm), the χ and pH values of the system, which have extrema at 40 and 50 °C.
A set of physicochemical methods was used to establish that aqueous solutions of chloracetophos fungicide and bactericide (
1
) in the range of calculated concentrations from 1•10
–18
to 1•10
–4
mol ...L
–1
are disperse systems in which a dispersed phase hundreds of nanometers in size is formed above and below a threshold concentration of 1•10
–9
mol L
–1
. Upon dilution disperse systems
1
are capable of non-monotonic changing the physicochemical properties and changing the toxicity profile (inhibition–stimulation) when influencing the growth and development of unicellular algae
Chlorella vulgaris
. The formation of domains and nanoassociates is accompanied by the appearance in the UV spectrum of an absorption band at 210–300 nm with a maximum at ~220 nm (
A
220
). The interrelated concentration dependences of
A
220
, the size of the dispersed phase, and electrical conductivity indicates that the observed spectral features of systems
1
are caused by the properties of the domains and nanoassociates.
Data on the antitumor activities of ferrocene compounds and the results concerning the mechanisms of their action published between 1995 and 2010 are discussed. The biomedical aspects of the study of ...ferrocene derivatives are briefly analyzed.
It was shown for the first time using a complex of physicochemical methods (dynamic and electrophoretic light scattering, nanoparticle tracking analysis, transmission electron and atomic force ...microscopy, conductometry, pH-metry) that in a temperature range of 25—60 °С (
S
)-lysine aqueous solutions (1•10
–17
—1•10
–2
mol L
–1
) are disperse systems, where the nature and parameters of the disperse phase change with dilution, which affects the nonmonotonic dependences of the specific conductivity and pH of the system. Nanoassociates hundreds of nanometers in size were found to contain ordered water structures that are responsible for the size and ζ-potential of the disperse phase in the aqueous systems and also participate in the formation of spatially organized systems on the support.
It has been shown for the first time that aqueous solutions of metaphos pesticide (1×10
–4
–1×10
–17
mol/L) are disperse systems, the disperse phase undergoing rearrangement upon dilution. The ...relationship between nonmonotonic concentration dependences of the nanoassociate size, physico-chemical properties of the systems, and their action on higher plants and hydrocoles has been found.
PDF
