Delphinidin is a flavonoid belonging to dietary anthocyanidin family that has been reported to possess diverse anti-tumoral activities. However, the effects of delphinidin on colorectal cancer (CRC) ...cells and the underlying mechanisms are not fully understood. Thus, we aimed to investigate the anti-cancer activity of delphinidin in CRC cells and the underlying molecular mechanisms. The effects of delphinidin on the viability, metastatic characteristics, signaling, and microRNA (miR) profile of human CRC cell lines used were analyzed. In vivo metastasis was also evaluated using xenograft animal models. Our findings showed that delphinidin (<100 μM) inhibited the colony formation of DLD-1, SW480, and SW620 cells, but non-significantly affected cell viability. Delphinidin also suppressed the migratory ability and invasiveness of the tested CRC cell lines, downregulated integrin αV/β3 expression, inhibited focal adhesion kinase (FAK)/Src/paxillin signaling, and interfered with cytoskeletal construction. Analysis of the miR expression profile revealed a number of miRs, particularly miR-204-3p, that were significantly upregulated and downregulated by delphinidin. Abolishing the expression of one upregulated miR, miR-204-3p, with an antagomir restored delphinidin-mediated inhibition of cell migration and invasiveness in DLD-1 cells as well as the αV/β3-integrin/FAK/Src axis. Delphinidin also inhibited the lung metastasis of DLD-1 cells in the xenograft animal model. Collectively, these results indicate that the migration and invasion of CRC cells are inhibited by delphinidin, and the mechanism may involve the upregulation of miR-204-3p and consequent suppression of the αV/β3-integrin/FAK axis. These findings suggest that delphinidin exerts anti-metastatic effects in CRC cells by inhibiting integrin/FAK signaling and indicate that miR-204-3p may play an important role in CRC metastasis.
A mild, facile, and metal-free approach via the N-heterocyclic carbene-catalyzed SNAr reaction between aryl aldehydes with perfluoroarenes to obtain the coveted functional perfluorinated ...diarylmethanones is disclosed. This method accommodates a diverse substrate range and exhibits notable tolerance toward various functional groups. Our success in modifying biologically relevant molecules, crafting a fully fluorinated bioisosteric analogue of drug candidate D1, and highlighting the potential of these ketones as valuable electrolyte additives for lithium-ion batteries (LIBs) underscores the versatility of our methodology.
This paper details fronthaul optical links using sub-Nyquist sampling rate analog-to-digital converters (ADCs) for Beyond fifth generation (B5G) and 6G sub-THz massive multiple-input multiple-output ...(Ma-MIMO) beamforming. Unlike Common Public Radio Interface (CPRI) using high speed ADCs in current fronthaul link, the proposed scheme involves ADCs operating at sub-Nyquist sampling rate for each antenna element. Based on pre-allocated relative time delays, pre-processed orthogonal frequency-division multiplexing (OFDM) signals sent from a baseband unit (BBU) can be deaggregated to different Ma-MIMO OFDM signals by sub-Nyquist sampling rate ADCs. In experiments, we assume that each remote radio unit (RRU) is equipped with 32/64 antenna elements and 32/64 ADCs operating at 1/32 and 1/64 of the Nyquist sampling rate. Furthermore, the received Ma-MIMO OFDM signal is then up-converted to 100-GHz for wireless transmission and defined as Ma-MIMO RF OFDM signal. We simulate the 32/64 antenna elements transmission scenario by individually transmit and demodulate each Ma-MIMO RF OFDM signal with 32/64 times of point-to-point antenna transmission. The error vector magnitude (EVM) and signal-to-noise ratio (SNR) of each received Ma-MIMO RF OFDM signal are less than 8% and 26 dB, respectively. And the total received 64 Ma-MIMO RF OFDM signals will require line rate as high as 393.6374 Gb/s according to CPRI option-7. Notably, the proposed scheme reduces the requirement of sampling rate and enables all the Ma-MIMO OFDM signals at baseband without the insertion of guard band. Thus, the proposed scheme can reduce the complexity of signal deaggregation and power consumption in the demodulation process, leading to an improvement in cost efficiency.
Polarization-dependent hard X-ray excited optical luminescence (XEOL) was used to study not only the optical properties but also the crystallographic orientations of a non-polar a-plane ZnO wafer. In ...addition to a positive-edge jump and extra oscillations in the near-band-edge (NBE) XEOL yield, we observed a blue shift of the NBE emission peak that follows the polarization-dependent X-ray absorption near-edge structure (XANES) as the X-ray energy is tuned across the Zn K-edge. This NBE blue shift is caused by the larger X-ray absorption, generating higher free carriers to reduce the exciton-LO phonon coupling, which causes a decrease in the exciton activation energy. The extra oscillations in XANES and XEOL as the polarization is set parallel to the c-axis is attributed to simultaneous excitations of the Zn 4p - O 2pπ -bond along the c-axis and the bilayer σ-bond, whereas only the σ-bond is excited when the polarization is perpendicular to the c-axis. The polarization-dependent XEOL spectra can be used to determine the crystallographic orientations.
The C-S activation and sulfur removal from native thiols is challenging, which limits their application as feedstock materials in organic synthesis despite their natural abundance. Herein, we ...introduce a per-/polyfluoroaryl moiety, which serves as a redox-active scaffold, into sp
-hybridized thiols to activate the C-S bond. Using a Ni catalyst with MgBr
as an additive, the S group can be removed to yield an aliphatic radical that can react with an aryl halide in a reductive cross-coupling.
The mortality-to-incidence ratio (MIR) is a marker that reflects the clinical outcome of cancer treatment. MIR as a prognostic marker is more accessible when compared with long-term follow-up ...survival surveys. Theoretically, countries with good health care systems would have favorable outcomes for cancer; however, no report has yet demonstrated an association between gallbladder cancer MIR and the World's Health System ranking.
We used linear regression to analyze the correlation of MIRs with the World Health Organization (WHO) rankings and total expenditures on health/gross domestic product (e/GDP) in 57 countries selected according to the data quality.
The results showed high crude rates of incidence/mortality but low MIR in more developed regions. Among continents, Europe had the highest crude rates of incidence/mortality, whereas the highest age-standardized rates (ASR) of incidence/mortality were in Asia. The MIR was lowest in North America and highest in Africa (0.40 and 1.00, respectively). Furthermore, favorable MIRs were correlated with good WHO rankings and high e/GDP (p = 0.01 and p = 0.030, respectively).
The MIR variation for gallbladder cancer is therefore associated with the ranking of the health system and the expenditure on health.
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•Roselle flower polyphenols suppressed motility of colon cancer cell DLD-1.•Roselle flower polyphenols inhibited focal adhesion kinase (FAK), CD44/c-MET and the associated ...signaling.•Roselle flower polyphenols in vivo lowered p-FAK, CD44, and c-MET.•Roselle flower polyphenols in vivo inhibited liver metastasis of DLD-1 cell.
Hibiscus sabdariffa extracts have multiple bioactivities. Here, we aimed to investigate the anti-metastatic effects of a polyphenol-enriched extract of Hibiscus sabdariffa (HPE) on colon cancer cells. We observed that HPE (≤3 mg/mL) significantly inhibited the cell migration and invasion of human colorectal cancer (CRC) cell DLD-1. In parallel, HPE altered cytoskeletal structure, downregulated the MMP-2, MMP-9, and uPA expression, increased TIMP2, and suppressed FAK and CD44/c-MET signaling in DLD-1 cells. Moreover, we also explored the in vivo anti-metastatic effect of HPE and found that HPE was able to inhibit the DLD-1 cells metastasizing to liver in xenograft animal model. Taken together, our findings indicate that HPE can both in vitro and in vivo inhibit the metastasis of DLD-1 cell, which may associate with the suppression of FAK and CD44/c-MET signaling, suggesting that HPE might be beneficial to the CRC patients receiving chemotherapy.
This study is aimed at estimating the unbiased effectiveness of population-based breast cancer service screening based on case survival information alone rather than large-scale individual screening ...data pursuant to the intention-to-treat principle of a randomized-controlled trial.
A novel time-dependent switched design with two modalities of cancer detection (screen-detected vs clinically detected) was proposed to evaluate the effectiveness of breast cancer screening. We used data on 767 patients from Kopparberg in the Swedish Two-County trial and on 78 587 patients in the Taiwan population-based service screening. We estimated the relative rate of the screen-detected vs the clinically detected with adjustment for both truncation and lead-time biases. The absolute effectiveness in terms of the number needed to screen (NNS) for averting one death from breast cancer was estimated.
The relative rate of effectiveness was estimated as 33%, which was consistent with the 37% reported from the original Swedish randomized-controlled trial. The corresponding estimate for the Taiwan screening programme was 42%, which was also very close to that estimated using individual screening history data (41%). Both relative estimates were further applied to yield 446 and 806 of NNS for averting one death from breast cancer for the corresponding two data sets.
The proposed time-dependent switched design and analysis with two modalities of case survival information provides a very efficient means for estimating the unbiased estimates of relative and absolute effectiveness of population-based breast cancer service screening dispensing with a large amount of individual screening history data.
Ovarian cancer treatment remains a challenge and targeting cancer stem cells presents a promising strategy. Niclosamide is an "old" antihelminthic drug that uncouples mitochondria of intestinal ...parasites. Although recent studies demonstrated that niclosamide could be a potential anticancer agent, its poor water solubility needs to be overcome before further preclinical and clinical investigations can be conducted. Therefore, we evaluated a novel nanosuspension of niclosamide (nano-NI) for its effect against ovarian cancer. Nano-NI effectively inhibited the growth of ovarian cancer cells in which it induced a metabolic shift to glycolysis at a concentration of less than 3 μM in vitro and suppressed tumor growth without obvious toxicity at an oral dose of 100 mg/kg in vivo. In a pharmacokinetic study after oral administration, nano-NI showed rapid absorption (reaching the maximum plasma concentration within 5 min) and improved the bioavailability (the estimated bioavailability for oral nano-NI was 25%). In conclusion, nano-NI has the potential to be a new treatment modality for ovarian cancer and, therefore, further clinical trials are warranted.
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